Optimizing Selectivity and Ion Utilization for Trapped Ion Mobility Spectrometry for Enhanced DIA Performance

ASMS2025, TP192 Benchmarking diagonal-PASEF data acquisition for high-throughput proteomics applications Christopher Below1, Oliver M. Bernhardt1, Stephanie Kaspar-Schoenefeld2, Roland Bruderer1, Shourjo Ghose3, Tejas Gandhi1, Jonathan Krieger4, Markus Lubeck2, Lukas Reiter1 More details: 1Biognosys AG, Zurich, Switzerland, 2Bruker Daltonics GmbH & Co…

US HUPO 2024 Deep proteome coverage at scale combined with reproducible quantitation on the timsTOF HT Stephanie Kaspar-Schoenefeld1, Andreas Schmidt1, Markus Lubeck1, Pierre-Olivier Schmit2, Torsten Mueller1 and Narayanaganesh Balasubramanian3 (A) (B) Fig. 1: Reproducible and in-depth protein identification from yeast…

dia-PASEF® applied on different gradient lengths Data Independent Acquisition (DIA) workflows have gained in popularity as they overcome the issue of stochastic selection of peptide precursors encountered in typical data-dependent approaches (DDA) and thereby promise reproducible and accurate protein identification…

diaPASEF: label-free quantification of highly complex proteomes The timsTOF Pro with diaPASEF provides reproducible and accurate qualitative and quantitative results in complex proteomics samples – making it perfectly suited for data-independent acquisition approaches. Abstract Data-independent acquisition (DIA) promises reproducible and…