Benchmarking diagonal-PASEF data acquisition for high-throughput proteomics applications

ASMS2025, TP202 Optimizing Selectivity and Ion Utilization for Trapped Ion Mobility Spectrometry for Enhanced DIA Performance Markus Lubeck1, Andreas Schmidt1, Stephanie Kaspar-Schoenefeld1, Matt Albano2, Cory Lytle2, Dijana Vitko2, Matthew Willetts2, Daniel Hornburg3 1Bruker Daltonics GmbH & Co. KG, Bremen, Germany,…

diaPASEF: label-free quantification of highly complex proteomes The timsTOF Pro with diaPASEF provides reproducible and accurate qualitative and quantitative results in complex proteomics samples – making it perfectly suited for data-independent acquisition approaches. Abstract Data-independent acquisition (DIA) promises reproducible and…

ASMS 2025, THP 663 Scaling-up low input spatial proteomics using Evosep Eno on the timsUltra AIP 1,000 30,000 µm2 regions: Epithelial (CDH1), B-cell (CD19) T-cell (CD3), Mixed (B / T-cell) Evosep Eno timsUltra AIP diaPASEF Spectronaut 19 1 2 3…

Parallel accumulation – serial fragmentation combined with data-independent acquisition (diaPASEF) The timsTOF Pro with diaPASEF enables deeper proteome coverage in a single 4D shotgun proteomics experiment, with highly reproducible qualitative and quantitative results – making it a near-ideal mass analyzer…