Deep proteome coverage at scale combined with reproducible quantitation on the timsTOF HT

ASMS2025, TP197 Assessment of a narrow-window dia-PASEF method for high-throughput proteomics Stephanie Kaspar-Schoenefeld1, Jonathan Krieger2, Savannah Snyder3, Markus Lubeck1, 1Bruker Daltonics GmbH & Co KG, Bremen, Germany Fig. 1 Overview narrow-window 2Bruker Ltd., Milton, ON dia-PASEF method. To optimize deep…

dia-PASEF® applied on different gradient lengths Data Independent Acquisition (DIA) workflows have gained in popularity as they overcome the issue of stochastic selection of peptide precursors encountered in typical data-dependent approaches (DDA) and thereby promise reproducible and accurate protein identification…

ASMS2025, TP202 Optimizing Selectivity and Ion Utilization for Trapped Ion Mobility Spectrometry for Enhanced DIA Performance Markus Lubeck1, Andreas Schmidt1, Stephanie Kaspar-Schoenefeld1, Matt Albano2, Cory Lytle2, Dijana Vitko2, Matthew Willetts2, Daniel Hornburg3 1Bruker Daltonics GmbH & Co. KG, Bremen, Germany,…

diaPASEF: label-free quantification of highly complex proteomes The timsTOF Pro with diaPASEF provides reproducible and accurate qualitative and quantitative results in complex proteomics samples – making it perfectly suited for data-independent acquisition approaches. Abstract Data-independent acquisition (DIA) promises reproducible and…