diaPASEF: label-free quantification of highly complex proteomes

Parallel accumulation – serial fragmentation combined with data-independent acquisition (diaPASEF) The timsTOF Pro with diaPASEF enables deeper proteome coverage in a single 4D shotgun proteomics experiment, with highly reproducible qualitative and quantitative results – making it a near-ideal mass analyzer…

dia-PASEF® applied on different gradient lengths Data Independent Acquisition (DIA) workflows have gained in popularity as they overcome the issue of stochastic selection of peptide precursors encountered in typical data-dependent approaches (DDA) and thereby promise reproducible and accurate protein identification…

Accurate Label-Free Protein Quantitation on the timsTOF Pro with 4D-Proteomics™ The timsTOF Pro platform brings together two unique technologies, namely Trapped Ion Mobility Spectrometry (TIMS) mode operation at the front-end and Parallel Accumulation Serial Fragmentation (PASEF [1]) for data acquisition.…

US HUPO 2024 Deep proteome coverage at scale combined with reproducible quantitation on the timsTOF HT Stephanie Kaspar-Schoenefeld1, Andreas Schmidt1, Markus Lubeck1, Pierre-Olivier Schmit2, Torsten Mueller1 and Narayanaganesh Balasubramanian3 (A) (B) Fig. 1: Reproducible and in-depth protein identification from yeast…