Optimizing IP-RP CRISPR sgRNA Purification Passes With High Efficiency Oligonucleotide Certified BEH 300 Å C18 5 μm Preparative Sorbent

Application Note CRISPR Single Guide RNA Characterization by IP- RP-LC-MS with a Premier Oligonucleotide BEH 300 Å C18 Column Maissa M. Gaye, Chris Knowles, Balasubrahmanyam Addepalli, Matthew A. Lauber Waters Corporation Abstract The high speed, low cost, and reduced need…

Application Note Reliable Purification of Full-Length Product and Enhanced Isolation and Detection of Impurities With Ready to Use XBridge Premier Oligonucleotide BEH™ C18 OBD™ Preparative Columns Michael Miller1, Paul Lefebvre1, Makda Araya2, Balasubrahmanyam Addepalli2, Mandana Fasth3, Christian Reidy2, Matthew Lauber2…

Application Note Method Development for Preparative Purification of Long Oligonucleotides Alexander Todorova, Stella VerkhnyatSkayaa, Imke Nijdama, Martin Gilarb, Mandana Fasthb a ProQR Therapeutics, Leiden, The Netherlands b Waters Corporation, Milford MA, United States b Waters Corporation, Stockholm, Sweden Published on…

Application Note Studying Mobile Phases for HILIC UV/MS Analysis of Oligonucleotide Therapeutics including an siRNA Duplex, a Lipid Conjugated ASO, and a CRISPR sgRNA Szabolcs Fekete, Makda Araya, Balasubrahmanyam Addepalli, Matthew A. Lauber Waters Corporation Abstract Hydrophilic interaction liquid chromatography…