Method Development for Preparative Purification of Long Oligonucleotides

Application Note Reliable Purification of Full-Length Product and Enhanced Isolation and Detection of Impurities With Ready to Use XBridge Premier Oligonucleotide BEH™ C18 OBD™ Preparative Columns Michael Miller1, Paul Lefebvre1, Makda Araya2, Balasubrahmanyam Addepalli2, Mandana Fasth3, Christian Reidy2, Matthew Lauber2…

Application Note Increasing the Productivity of Oligonucleotide Purification through Column Scaling and Method Optimization Michael Miller, Paul Lefebvre, Andrew Leightner, Matthew A. Lauber, Mandana Fasth, Paul D. Rainville, Darcy Shave Neopharm Boston Inc, Milford MA, USA, Waters Corporation For research…

Application Note Optimizing IP-RP CRISPR sgRNA Purification Passes With High Efficiency Oligonucleotide Certified BEH 300 Å C18 5 µm Preparative Sorbent Amy Howie, Mandana Fasth, Chris Boggess, Matthew A. Lauber Oligo Factory, Waters Corporation Abstract CRISPR technology, which utilizes single-guide…

[ APPLICATION NOTEBOOK ] APPLICATION SOLUTIONS FOR OLIGONUCLEOTIDES Table of Contents Introduction – UPLC Analysis of Synthetic Oligonucleotides......................................................................................................................... 4 [ HPLC & UPLC Separations ] Real-Time Analysis of RNAi Duplexes ....................................................................................................................................................................... 8 Semi-Preparative Scale Single-Stranded RNA Purification .......................................................................................................................... 11 Oligonucleotide…