CRISPR Single Guide RNA Characterization by IP- RP-LC-MS with a Premier Oligonucleotide BEH 300 Å C18 Column

Application Note RNA Digestion Product Mapping Using an Integrated UPLC-MS and Informatics Workflow Catalin E. Doneanu, Chris Preston, Matt Gorton, Tatiana Johnson, Bala Addepalli, Ying Qing Yu Waters Corporation Abstract LC-MS analysis is an indispensable tool for single-guide RNA (sgRNA)…

Application Note Oligo Mapping of sgRNA Digests: Leveraging Xevo MRT Mass Spectrometer Performance and Streamlining Data Analysis Catalin Doneanu, Alexandre F Gomes, Tatiana Johnston, Chris Preston, Matt Gorton, Bala Addepalli, Scott Berger, Ying Qing Yu Waters Corporation, Milford, MA, United…

Application Note Optimizing IP-RP CRISPR sgRNA Purification Passes With High Efficiency Oligonucleotide Certified BEH 300 Å C18 5 µm Preparative Sorbent Amy Howie, Mandana Fasth, Chris Boggess, Matthew A. Lauber Oligo Factory, Waters Corporation Abstract CRISPR technology, which utilizes single-guide…

Application Note Sequence Mapping of sgRNA Digests: Leveraging Xevo™ MRT Mass Spectrometer Performance and Streamlining Data Analysis with MAP Sequence 2.0 Catalin Doneanu, Alexandre F Gomes, Tatiana Johnston, Chris Preston, Matt Gorton, Bala Addepalli, Scott Berger, Ying Qing Yu Waters…