LC-MS-based host cell protein (HCP) identification and monitoring during biopharmaceutical downstream process development

APPLICATION NOTE 74109 Comprehensive identification and label-free quantitation of host cell protein contaminants using BioPharma Finder 4.1 software Authors: Silvia Millán-Martín,1 Sara Carillo,1 Jonathan Bones1, Jennifer Sutton2 National Institute for Bioprocessing Research and Training (NIBRT), Dublin, Ireland 1 2 Thermo…

Applying UHPLC HRAM MS-MS/MS method to assess host cell protein clearance during the purification process development of therapeutic mAbs Reiko Kiyonami1, Ying Chen2, AI De Leon2, Min Du1, 1Thermo Fisher Scientific, Lexington, MA; 2Thermo Fisher Scientific, Bedford, MA Introduction Mass…

APPLICATION NOTE 73412 Residual host cell protein analysis of NISTmAb: From simplified sample preparation to reliable results Authors: Amy Claydon, Phil Widdowson, and Andrew Williamson Thermo Fisher Scientific, Hemel Hempstead, UK Keywords: Host cell protein assay, HCP, monoclonal antibody, mAb,…

Increasing sensitivity of high throughput host cell protein analysis on a novel highresolution accurate mass platform Eugen Damoc1; Tabiwang N. Arrey1; Anna Pashkova1; Eduard Denisov1; Kai Scheffler2; Kristina Srzentić3 1Thermo Fisher Scientific (Bremen) GmbH, Bremen, Germany; 2Thermo Fisher Scientific, Germering,…