Applying UHPLC HRAM MS-MS/MS method to assess host cell protein clearance during the purification process development of therapeutic mAbs

APPLICATION NOTE 74162 LC-MS-based host cell protein (HCP) identification and monitoring during biopharmaceutical downstream process development Authors: Xiaoxi Zhang1, Zijuan Chen2, Bingnan Li2, Jennifer Sutton3, Min Du4 Thermo Fisher Scientific 1 Shanghai, China; 2 Bioprocess Design Center, Shanghai, China; 3…

High-sensitivity Host Cell Protein (HCP) analysis on a new Orbitrap Excedion Pro BioPharma hybrid mass spectrometer with optimized sample preparation Xiaoxi Zhang1, Reiko Kiyonami2, Jae Choi3, Bhavin Patel3, Cong Wang4, Peter Krueger4, Heiner Koch4, Min Du2. 1 Thermo Fisher Scientific,…

Rapid monitoring of mAb aggregates during the purification process development of therapeutic mAbs using a modified Orbitrap hybrid MS Reiko Kiyonami1, Ying Chen2, Nicole Zehethofer3, Catharina Crone3, AI De Leon2, Heiner Koch3, Min Du1 1Thermo Fisher Scientific, Lexington, MA; 2Thermo…

APPLICATION NOTE 73412 Residual host cell protein analysis of NISTmAb: From simplified sample preparation to reliable results Authors: Amy Claydon, Phil Widdowson, and Andrew Williamson Thermo Fisher Scientific, Hemel Hempstead, UK Keywords: Host cell protein assay, HCP, monoclonal antibody, mAb,…