Increasing sensitivity of high throughput host cell protein analysis on a novel high- resolution accurate mass platform

APPLICATION NOTE 73412 Residual host cell protein analysis of NISTmAb: From simplified sample preparation to reliable results Authors: Amy Claydon, Phil Widdowson, and Andrew Williamson Thermo Fisher Scientific, Hemel Hempstead, UK Keywords: Host cell protein assay, HCP, monoclonal antibody, mAb,…

High-sensitivity Host Cell Protein (HCP) analysis on a new Orbitrap Excedion Pro BioPharma hybrid mass spectrometer with optimized sample preparation Xiaoxi Zhang1, Reiko Kiyonami2, Jae Choi3, Bhavin Patel3, Cong Wang4, Peter Krueger4, Heiner Koch4, Min Du2. 1 Thermo Fisher Scientific,…

Confident LC-MS Identification of Low ppm Host Cell Proteins (HCPs) in Biotherapeutic Monoclonal Antibodies Amy J Claydon1, Phil Widdowson1, Andrew Williamson2, and Min Du3, Thermo Fisher Scientific, 1Runcorn, UK, 2Hemel Hempstead, UK, 3Cambridge, MA, USA Label-free quantification of NISTmAb HCPs…

Applying UHPLC HRAM MS-MS/MS method to assess host cell protein clearance during the purification process development of therapeutic mAbs Reiko Kiyonami1, Ying Chen2, AI De Leon2, Min Du1, 1Thermo Fisher Scientific, Lexington, MA; 2Thermo Fisher Scientific, Bedford, MA Introduction Mass…