In Depth Characterization of mAb Charge Variants with iCIEF Fractionation Followed by Peptide Mapping Analysis

Characterization of monoclonal antibodies and antibody drug conjugates using iCIEF-MS online coupling platform Xiaoxi Zhang1, Tiemin Huang2, Tony Chen2, Tao Bo3, Min Du4. 1 Thermo Fisher Scientific, Shanghai, China, 2 Advanced Electrophoresis Solutions Ltd., Cambridge, Ontario, Canada, 3 Thermo Fisher…

Imaged capillary isoelectric focusing – Mass Spectrometry (iCIEF- MS) online coupling for polatuzumab vedotin charge heterogeneity analysis using native MS Xiaoxi Zhang1, Tony Chen2, Tao Bo2, Tiemin Huang2, Min Du3. 1 Thermo Fisher Scientific, Shanghai, China, 2 Advanced Electrophoresis Solutions…

MauriceFlex Fractionation of charge variants accompanied by LC-MS and Digital SPR Analysis correlates Structure to the Function of a mAb Srinivasa Rao1, Samantha Ippoliti2, Ying Qing Yu2, Michael Piazza3, Chris Heger1 Bio-Techne1, San Jose, CA; Waters Corporation2, Milford, MA; Nicoya…

Application Note Charge Variant Characterization of Innovator and Biosimilar Drugs with MauriceFlex™ and BioAccord™ LC-MS System Samantha Ippoliti, Srinivasa Rao, Manisha Pratap, Ying Qing Yu, Chris Heger Waters Corporation, Bio-Techne Abstract This application note showcases an in-depth comparative analysis of…