Imaged capillary isoelectric focusing – Mass Spectrometry (iCIEF- MS) online coupling for polatuzumab vedotin charge heterogeneity analysis using native MS

Application note | 004167 Biopharma Charge variant analysis of cysteine-linked antibody-drug conjugates using an online multiple heart-cut 2DSCX-SEC-LC HRAM MS approach Authors Application benefits Xuepu Li¹, Xiaoxi Zhang¹, Maria Grübner², • The online multiple heart-cut two-dimensional liquid chromatography (2D-LC) system…

Characterization of monoclonal antibodies and antibody drug conjugates using iCIEF-MS online coupling platform Xiaoxi Zhang1, Tiemin Huang2, Tony Chen2, Tao Bo3, Min Du4. 1 Thermo Fisher Scientific, Shanghai, China, 2 Advanced Electrophoresis Solutions Ltd., Cambridge, Ontario, Canada, 3 Thermo Fisher…

Biopharma High-sensitive multi-attribute analysis of ADCs under native conditions by using an online multiple heart-cut 2D-LC-HRAM mass spectrometry system Frank Steiner1, Maria Gruebner1, Xuepu Li2, Xiaoxi Zhang2, Min Du3 1Thermo Fisher Scientific, Germering, Germany, 2Thermo Fisher Scientific, Shanghai, China, 3Thermo…

Increased sensitivity and throughput for native intact mass analysis of mAb and ADCs using an online buffer exchange column Xiaoxi Zhang1, Weijing Liu2, Xuepu Li1, Min Du3 1 Thermo Fisher Scientific, Shanghai, China, 2 Thermo Fisher Scientific, San Jose, CA,…