Next-generation MALDI top-down sequencing of protein biotherapeutics – expanding the scope of timsTOF technology

Recombinant SARS-CoV-2 Receptor Binding Domain: Comprehensive Top-Down Sequence Confirmation, Curation and O-Glycosylation Site Determination Know your SARS-CoV-2 antigen structure before it´s use in research and diagnostics Abstract The COVID pandemic dramatically influences our life and the demand remains very high…

AS M S 2 0 2 5 W P - 5 7 8 In-Depth Protein Sequence Verification by TIMS-enabled MALDI Top-Down Sequencing Detlev Suckau1, George Alevizos2, Georgia Orfanoudaki2, Guillaume NH3 Evers1, Arndt Asperger1 1Bruker Daltonics GmbH & Co. KG, Bremen,…

Extensive LC-Top-Down MS Sequence Confirmation of the NISTmAb Reference Material 8671 Characterization of therapeutic antibodies aims on primary sequence validation, localization of modifications such as glycan profiles and the determination of disulfide bond status of the target molecule. Introduction Typically,…

Rapid-Turnaround Top-Down Sequence Verification and Characterization of Modified Proteins and Oligonucleotides Using a Benchtop MALDI-TOF/TOF Instrument Sinduri Vuppala1, Savannah Snyder1, Arndt Asperger2 1 Bruker Scientific LLC, Billerica, MA, USA 2 Bruker Daltonics GmbH & Co. KG, Bremen, Germany Results i)…