In-Depth Protein Sequence Verification by TIMS-enabled MALDI Top-Down Sequencing

Extensive LC-Top-Down MS Sequence Confirmation of the NISTmAb Reference Material 8671 Characterization of therapeutic antibodies aims on primary sequence validation, localization of modifications such as glycan profiles and the determination of disulfide bond status of the target molecule. Introduction Typically,…

Next-generation MALDI top-down sequencing of protein biotherapeutics – expanding the scope of timsTOF technology Next-generation MALDI top-down sequencing (MALDI-TDS) improves top-down sequence analysis of biopharmaceutical proteins, as demonstrated in this note for adalimumab subunits, recombinant SARS-CoV-2 S-glycoprotein RBD expression products,…

Complete de novo sequencing of a human immunoglobulin G using multiple enzyme digests and LC-ESI-QTOF-MS/MS analysis Detailed method for antibody de novo sequencing Abstract The development of monoclonal antibody-based biotherapeutics requires the determination of the full protein sequence. The cloning…

AS M S 2 0 2 6 – TH P 6 4 9 Filling the gaps: multi-experiment de novo tag integration for confident top-down protein characterization in OmniScape Learn more George Alevizos1; Mariangela Kosmopoulou1; Georgia Orfanoudaki1; Detlev Suckau2; Athanasios Smyrnakis1;…