Recombinant SARS-CoV-2 Receptor Binding Domain: Comprehensive Top-Down Sequence Confirmation, Curation and O-Glycosylation Site Determination

Next-generation MALDI top-down sequencing of protein biotherapeutics – expanding the scope of timsTOF technology Next-generation MALDI top-down sequencing (MALDI-TDS) improves top-down sequence analysis of biopharmaceutical proteins, as demonstrated in this note for adalimumab subunits, recombinant SARS-CoV-2 S-glycoprotein RBD expression products,…

Extensive LC-Top-Down MS Sequence Confirmation of the NISTmAb Reference Material 8671 Characterization of therapeutic antibodies aims on primary sequence validation, localization of modifications such as glycan profiles and the determination of disulfide bond status of the target molecule. Introduction Typically,…

Rapid-Turnaround Top-Down Sequence Verification and Characterization of Modified Proteins and Oligonucleotides Using a Benchtop MALDI-TOF/TOF Instrument Sinduri Vuppala1, Savannah Snyder1, Arndt Asperger2 1 Bruker Scientific LLC, Billerica, MA, USA 2 Bruker Daltonics GmbH & Co. KG, Bremen, Germany Results i)…

How to Determine Glycan Profiles of Biopharmaceuticals from Peptide Mapping Data A new and sensitive approach combining BioPharma Compass, PASEF and VIP-HESI Abstract Glycosylation is a common critical quality attribute (CQA) of therapeutic proteins and needs to be characterized during…