<p><strong>Post-translational modifications (PTMs) are where so much of biology’s complexity lives, but they are also some of the hardest molecular features to analyze with confidence. Phosphorylation, asymmetric dimethylarginine (ADMA), and O-GlcNAcylation (O-GlcNAc) often occur at low abundance, can be highly labile, and frequently require precise site localization to understand their biological meaning. This</strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_004377_ov_phosphorylation_ptm_etd_excedion_pro_tn004377_na_en_71f681655f.pdf"><strong> technical note </strong></a><strong>shows how the </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1958"><strong>Thermo Scientific Orbitrap Excedion Pro Hybrid Mass Spectrometer</strong></a><strong> helps address those challenges by combining fast, sensitive performance with complementary </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7217"><strong>HCD and EThcD fragmentation</strong></a><strong> workflows.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Phosphorylation_ADMA_and_O_Glc_N_Ac_Analysis_on_the_Orbitrap_Excedion_Pro_Mass_Spectrometer_d24a5de98e.jpg" alt="Thermo Fisher Scientific: Phosphorylation, ADMA, and O-GlcNAc Analysis on the Orbitrap Excedion Pro Mass Spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Phosphorylation_ADMA_and_O_Glc_N_Ac_Analysis_on_the_Orbitrap_Excedion_Pro_Mass_Spectrometer_d24a5de98e.jpg 245w," sizes="100vw" width="1024" height="512"></p><p>Using enriched PTM samples and automated data-dependent acquisition, the study demonstrates that the <strong>Orbitrap Excedion Pro MS</strong> can deliver both depth and confidence across multiple PTM classes. For phosphorylation analysis, the platform identified 5,892 unique phosphosites from IMAC-enriched samples and <strong>2,941 </strong>unique phosphotyrosine sites after phosphotyrosine enrichment, highlighting strong performance for both broad and highly targeted signaling studies. Just as importantly, the data show that HCD and EThcD provide complementary value, with each fragmentation method contributing unique site identifications and helping resolve ambiguous assignments.</p><p>The note also highlights the value of EThcD for more analytically demanding PTMs. In ADMA-enriched samples, EThcD delivered more ADMA peptide-spectrum matches and more unique modified peptides than HCD, supporting improved confidence in site characterization. For O-glycopeptides, the Orbitrap Excedion Pro MS uses an intelligent HCD-product-dependent-EThcD strategy that triggers EThcD only when oxonium ions are detected, improving productivity while preserving the fragmentation detail needed for confident glycosite localization.</p><p>Overall, this technical note shows how <strong>Orbitrap Excedion Pro MS </strong>enables researchers to go deeper into PTM biology with greater sensitivity, better site confidence, and smarter acquisition. For scientists studying signaling, regulation, and disease mechanisms, it presents a powerful approach for expanding PTM coverage without compromising analytical rigor.</p><blockquote><h5><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_004377_ov_phosphorylation_ptm_etd_excedion_pro_tn004377_na_en_71f681655f.pdf"><span><strong>Download the technical note</strong></span> "<strong>Advancing phosphorylation, ADMA, and O-GlcNAc PTM analysis with HCD and EThcD on the Orbitrap Excedion Pro mass spectrometer</strong>"</a></h5></blockquote><p><strong>Visit us on&nbsp;</strong><a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/"><strong><u>LinkedIn:</u></strong></a>&nbsp;#PTM #OrbitrapExcedionPro #glycosylation #phosphorylation</p>

Phosphorylation, ADMA, and O-GlcNAc Analysis on the Orbitrap Excedion Pro Mass Spectrometer

<h3><strong>Refractive Index (RI) Detection in HPLC – Making Invisible Compounds Visible</strong></h3><p><strong>When HPLC detectors are discussed, UV-Vis absorbance detection is often the first technique that comes to mind. Its popularity is easy to understand: it is sensitive, selective, and suitable for a wide range of compounds. But what happens when the analyte does not absorb ultraviolet or visible light?</strong></p><p>This is where the <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2429"><strong>Refractive Index Detector (RID)</strong></a> becomes particularly useful. Reliable, versatile, and often described as a “universal detector” for HPLC, RI detection can reveal compounds that remain invisible to many optical detectors. For substances such as sugars, polymers, lipids, or alcohols, it is often an important analytical option.</p><p>Let’s look at how RI detection works, where it performs best, what its main limitations are, and how to achieve stable, reproducible results.</p><h4><strong>How RI Detection Works</strong></h4><p>When light passes from one medium into another, its path changes direction, or refracts, depending on the phase velocity and angle of incidence. This phenomenon is described by <i>Snell’s law</i>, with the extent of refraction determined by the refractive index (RI) of the medium (Figure 1).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_Refraction_of_light_at_the_interface_between_two_media_with_different_refractive_indices_baa4aad28c.webp" alt="KNAUER: Figure 1: Refraction of light at the interface between two media with different refractive indices, where n₁ &gt; n₂. Since the velocity is lower in the second medium (v₂ &lt; v₁), the angle of refraction α₂ is less than the angle of incidence α₁." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_Refraction_of_light_at_the_interface_between_two_media_with_different_refractive_indices_baa4aad28c.webp 238w," sizes="100vw" width="1024" height="670"></p><p>In HPLC, an RI detector monitors <strong>small changes in the refractive index of the mobile phase as compounds elute from the column</strong>. Because each substance has its own refractive index, even relatively small changes in the composition of the column effluent can generate a measurable response.</p><p>Modern RI detectors generally use a <strong>differential optical design</strong> (Figure 2). Two optical paths are continuously compared: one passes through a <strong>reference cell</strong> containing pure mobile phase, while the other passes through a <strong>sample cell</strong> containing the column effluent, i.e. mobile phase plus analyte.</p><p>A light source, commonly an LED or tungsten lamp, illuminates both cells. Changes in refractive index alter the path of the light, and a photodiode detects the resulting deviation.</p><p>When only mobile phase is present, the two optical paths are effectively identical and no significant detector response is produced. As an analyte enters the sample cell, however, the refractive index changes. The light beam is slightly deflected, creating an <strong>optical imbalance</strong> and consequently a change in light intensity.</p><p>The detector converts this change into an electrical signal, which appears as a chromatographic peak. Because the response is related to analyte concentration, RI detection can also be used for quantitative analysis.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Schematic_of_a_differential_RI_detector_with_dual_flow_cell_6299f55b17.webp" alt="KNAUER: Figure 2: Schematic of a differential RI detector with dual flow cell. The sample cell carries column effluent; the reference cell contains pure mobile phase. A light beam passes through both and forms a slit image on a photodiode. Any analyte-induced change in refractive index alters the beam’s path, shifting the image on the photodiode and generating a signal peak." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Schematic_of_a_differential_RI_detector_with_dual_flow_cell_6299f55b17.webp 245w," sizes="100vw" width="1024" height="524"></p><p>For reliable measurements, the reference cell must always contain the same mobile phase as the sample cell, but without analyte. It therefore needs to be refreshed regularly with clean mobile phase, commonly using a switching valve and/or T-piece (Figure 3).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Flow_path_in_the_AZURA_RID_2_1_L_during_analysis_left_and_flushing_right_e6bc30acef.webp" alt="KNAUER: Figure 3: Flow path in the AZURA® RID 2.1L during analysis (left) and flushing (right). During analysis, solvent flows from the IN port through the heat exchanger and sample cell, then continues through the T piece and flush valve to the OUT port. During flushing, the valve redirects the flow through the T piece so the solvent passes a second heat exchanger and reference cell before exiting via the OUT port." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Flow_path_in_the_AZURA_RID_2_1_L_during_analysis_left_and_flushing_right_e6bc30acef.webp 245w," sizes="100vw" width="1024" height="410"></p><p>It is important to remember that refractive index depends on both <strong>light wavelength</strong> and <strong>medium density</strong>. The wavelength is fixed by the optical design of the detector, but density—and therefore refractive index—changes with <strong>temperature, pressure, and composition</strong>.</p><p>For this reason, RI detection is particularly sensitive to changes in operating conditions. Stable measurements depend heavily on maintaining a tightly controlled analytical environment.</p><h4><strong>Why Choose RI Detection?</strong></h4><p>RI detectors become especially valuable when other detector types cannot provide a useful response. They may be less sophisticated than some modern detection technologies, but several characteristics make them indispensable for particular applications.</p><h5><strong>Broad Applicability</strong></h5><p>An RID can detect virtually any compound that produces a change in the refractive index of the mobile phase. No UV-absorbing chromophore is required.</p><p>Because almost every dissolved substance changes refractive index to some degree, RI detection is commonly regarded as a <strong>universal detection technique</strong>. Typical analytes include sugars, alcohols, lipids, fatty acids, and polymers.</p><h5><strong>Non-Destructive Detection</strong></h5><p>RI measurement does not destroy the analyte. This makes it useful in workflows where the separated compound needs to be collected or analyzed further, including preparative chromatography.</p><p>KNAUER’s <a target="_blank" rel="noopener noreferrer" href="https://store.knauer.net/shop/add38-azura-r-rid-2-1l-highflow-preparative-refractive-index-detector-62927?category=75">AZURA® RID 2.1L for preparative applications</a>, for example, supports flow rates up to 100 mL/min.</p><h5><strong>Simple and Reliable Operation</strong></h5><p>RI detectors are suitable for aqueous and many organic mobile phases as long as the solvent composition remains constant.</p><p>Under stable isocratic conditions, they are relatively straightforward to use, robust, and require comparatively little maintenance.</p><h5><strong>Direct Detection Without Derivatization</strong></h5><p>Analytes do not need to be derivatized or labelled before RI detection. If a compound is soluble in the mobile phase and produces a sufficient difference in refractive index, it can be detected directly.</p><p>This simplifies sample preparation and reduces additional handling steps that could introduce variability or error.</p><p>These characteristics make RI detection particularly attractive for routine applications such as lactose determination in milk or polymer purity testing.</p><h5><strong>Limitations of RI Detection</strong></h5><p>Universal detection comes with several practical compromises. RI detectors require highly stable conditions and can be considerably more sensitive to environmental and system changes than other common HPLC detectors.</p><h5><strong>High Temperature Sensitivity</strong></h5><p>Refractive index is strongly influenced by temperature. For aqueous solvents, the change in refractive index with temperature is approximately dn/dT ≈ 10⁻⁴/°C.</p><p>Even small temperature fluctuations can therefore produce baseline drift that may be larger than the analytical signal itself.</p><p>Modern RI detectors use thermostated compartments and heat exchangers to stabilize the temperature of the flow cell. This improves accuracy and reproducibility, although the heat exchangers introduce additional system volume that can contribute to peak broadening compared with UV detection.</p><h5><strong>Limited Compatibility With Gradient Elution</strong></h5><p>RI detection is essentially restricted to <strong>isocratic chromatography</strong>.</p><p>Changing the composition of the mobile phase during a gradient produces a much larger change in refractive index than the analyte itself. The resulting baseline shift can easily overwhelm chromatographic peaks.</p><p>A stable RI baseline therefore requires the sample and reference cells to contain the same mobile-phase composition throughout the analysis.</p><h5><strong>Lower Sensitivity</strong></h5><p>Compared with UV detection, RI detection is generally about one to two orders of magnitude less sensitive.</p><p>Typical detection limits are in the µg–mg/mL range, making RID less suitable for trace-level analysis or impurity profiling.</p><h5><strong>Limited Pressure Tolerance</strong></h5><p>RI flow cells can tolerate only moderate backpressure. When several detectors are connected in series, the RI detector should therefore normally be positioned last.</p><h5><strong>Longer Equilibration</strong></h5><p>RID systems require time to reach thermal and optical stability. Warm-up and equilibration can take hours before a sufficiently stable baseline is obtained.</p><h5><strong>Sensitivity to Baseline Disturbances</strong></h5><p>Because the detector responds to any change in refractive index, many factors can affect the baseline.</p><p>Temperature fluctuations, unstable flow or pressure, pump pulsation, air bubbles, inadequate solvent mixing, and impurities in the mobile phase can all lead to noise, drift, or false signals.</p><p>Clean operation and stable system conditions are therefore essential.</p><h4><strong>How to Get the Best Performance From an RI Detector</strong></h4><p>Successful RI detection can largely be summarized in one word: <strong>stability</strong>.</p><p>Small variations in temperature, flow, solvent composition, or pressure can quickly become visible in the chromatographic baseline. Careful system preparation and handling can therefore make a major difference.</p><h5><strong>Maintain a Stable Temperature</strong></h5><p>Thermal equilibration should be one of the first priorities.</p><p>Allow the detector, column, and mobile phase to equilibrate fully before beginning analysis, ideally for at least 60 minutes. A column oven can help maintain stable conditions, and the column and detector should be operated at the same temperature whenever possible.</p><p>Environmental influences such as direct sunlight, air currents, or day-to-night laboratory temperature fluctuations should also be minimized.</p><h5><strong>Use Isocratic Methods</strong></h5><p>Keep the mobile-phase composition constant throughout the run.</p><p>If the separation requires gradient elution, an RI detector is generally not the appropriate choice.</p><h5><strong>Degas the Mobile Phase and Keep the System Clean</strong></h5><p>Use well-degassed, particle-free mobile phases and make sure the LC system is properly primed.</p><p>An in-line degasser can help prevent bubble formation. Tubing, solvent reservoirs, and the entire flow path should also be kept clean because even small bubbles or contaminants can generate spikes, noise, or other baseline disturbances.</p><h5><strong>Match the Sample Solvent Carefully</strong></h5><p>Whenever possible, prepare samples directly in the mobile phase or in a solvent with very similar composition.</p><p>Large differences between the injection solvent and mobile phase can produce strong refractive-index disturbances around the void volume.</p><p>Filtering samples also helps reduce the risk of blockages and protects the detector flow cell.</p><h5><strong>Keep Flow and Pressure Stable</strong></h5><p>Maintain a constant flow rate and avoid abrupt pressure changes, excessive pump pulsation, or leaks.</p><p>A restriction capillary or back-pressure regulator positioned after the flow cell may help stabilize pressure, although these measures cannot compensate for noise caused by a poorly performing pump.</p><h5><strong>Maintenance and Practical Considerations</strong></h5><p>Flush the reference cell regularly with fresh mobile phase. The mobile phase itself should also be replaced periodically to reduce microbial growth or compositional changes caused by evaporation.</p><p>When changing solvent systems, flush the instrument with mutually miscible solvents and allow sufficient time for the new system to equilibrate fully.</p><p>Where appropriate, mobile-phase recycling or a low-flow standby mode can help reduce solvent consumption and shorten restart times between analytical sequences.</p><blockquote><p>👉 <i>Pro Tip: Keep an eye on the baseline. A stable baseline is one of the best signs that the RID has equilibrated properly. Negative peaks are not necessarily an error—they simply indicate that the analyte has a lower refractive index than the mobile phase.</i></p></blockquote><h5><strong>The Bottom Line</strong></h5><p>With careful control of temperature, mobile-phase composition, pressure, and cleanliness, an RI detector can provide stable baselines and consistent quantitative data.</p><p>Keep the system clean, maintain constant operating conditions, and allow sufficient equilibration time, and even this demanding HPLC “diva” can deliver highly reliable results.</p><h4><strong>Typical Applications: Where RI Detection Performs Best</strong></h4><p>RI detection is particularly useful for substances that do not absorb UV light strongly and therefore cannot be detected effectively using conventional UV/VIS detectors.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AI_Figure_4_Common_uses_of_RI_detection_in_HPLC_d84d8458dc.webp" alt="KNAUER / AI: Figure 4: Common uses of RI detection in HPLC" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AI_Figure_4_Common_uses_of_RI_detection_in_HPLC_d84d8458dc.webp 245w," sizes="100vw" width="1024" height="433"></p><p>Typical analytes include sugars, alcohols, fatty acids, polymers, lipids, and other small organic molecules without strong chromophores.</p><h5><strong>Some common application areas include:</strong></h5><ul><li><strong>Carbohydrate and sugar analysis</strong>, including determination of glucose, fructose, sucrose, or lactose in food and beverages. HPLC-RI has been used for <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/VFD_0195_sec_for_fingerprint_analysis_of_gummy_candies_ce5349cd95.pdf"><strong>product quality control of gummy candies</strong></a>, with further discussion available in this <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/article/7186"><strong>related blog post</strong></a>.</li><li>KNAUER’s AZURA® RID 2.1L has also been used for the <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/VFD_0194_d497a35278.pdf"><strong>simultaneous determination of sugars and organic acids in wine</strong></a>, an application explored further in the article <a target="_blank" rel="noopener noreferrer" href="https://www.knauer.net/blog/blog-9/the-hidden-science-in-your-glass-147">“The hidden science in your glass”</a>.</li><li><strong>Polymer and macromolecule characterization</strong> in GPC/SEC applications, for example when determining molecular-weight distributions. Examples include <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/4czsecf32d70a6d2yugpojtljg8rvp4vd4ur_05660bfad3.pdf"><strong>optimization of molecular-weight determination with the KNAUER AZURA® GPC/SEC system</strong></a> and <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/qpwsk0igeqca8b5bqunbjjqnwum09tucb83s_cc4dbbcec9.pdf"><strong>analysis of mucin proteins using an AZURA® SEC System</strong></a>.</li><li><strong>Bioprocessing</strong>, including monitoring fermentation products such as <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/VBS_0079_Characterization_of_fructans_in_fermentation_products_with_sec_bc8f294f0e.pdf"><strong>fructans</strong></a>.</li><li><strong>Pharmaceutical applications</strong>, for example analysis of excipients such as sugars and polyols, as well as identity and purity testing of APIs. Dextranes, which are used as carrier materials in pharmaceuticals and as thickening or moisturizing agents in cosmetics, can be <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/h2oikq51kfvn6ebxij6hs88pxupstfgdqfyd_3e4f29d933.pdf"><strong>characterized by SEC</strong></a>, as their properties depend strongly on molecular-weight distribution.</li><li><strong>Lipid and surfactant analysis</strong> for formulation development and quality control.</li><li><strong>Environmental analysis</strong>, including determination of selected organic and inorganic impurities in water samples.</li></ul><p>Overall, RI detection works best for analytes that are soluble, stable in solution, and compatible with straightforward isocratic separation conditions.</p><p>It is not the preferred solution for trace analysis, gradient chromatography, or workflows requiring direct MS coupling. Nevertheless, it remains a practical and dependable option for compounds that are difficult or impossible to monitor using UV or fluorescence detection.</p><h4><strong>Final Thoughts: A Detector That Still Deserves Attention</strong></h4><p>As analytical laboratories adopt increasingly sophisticated detection technologies, refractive index detection can appear relatively simple. Yet for many applications, that simplicity is exactly its strength.</p><p>For analytes such as sugars, alcohols, polymers, and other compounds without strong UV absorption, RID offers a direct, reliable, and non-destructive method of detection.</p><p>Its greatest challenges are its sensitivity to temperature and its incompatibility with gradient elution. Under carefully controlled isocratic conditions, however, RI detection remains a dependable workhorse for routine HPLC and GPC/SEC applications.</p><blockquote><p><i><strong>Pro tip: If your analyte seems invisible to conventional optical detection, a change of perspective may be all that is needed. RI detection could make it visible.</strong></i></p></blockquote><p>In the next article in the <strong>“Seeing is Believing – Detection Made Easy”</strong> series, we will focus on <strong>Fluorescence Detection (FLD)</strong>, a highly selective and sensitive technique for compounds that fluoresce naturally or can be derivatized to do so.</p>

Seeing is Believing – Detection Made Easy Part 4: Refractive Index Detector

News from LabRulezLCMS Library - Week 36, 2026

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Revolutionizing how you process, review, and report data, your compliance-enabled solution will take command of your data and empower you to spend less time on difficult manual processes and more time making fast, informed decisions to advance your science.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_waters_connect_for_Quantitation_The_MS_Quan_app_is_integrated_with_the_waters_connect_workflow_and_utility_apps_to_provide_a_seamless_efficient_workflow_for_quantitation_5a8779a387.jpg" alt="Waters: waters_connect for Quantitation: The MS Quan app is integrated with the waters_connect workflow and utility apps to provide a seamless, efficient workflow for quantitation." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_waters_connect_for_Quantitation_The_MS_Quan_app_is_integrated_with_the_waters_connect_workflow_and_utility_apps_to_provide_a_seamless_efficient_workflow_for_quantitation_5a8779a387.jpg 234w," sizes="100vw" width="1024" height="682"></p><h4><strong>Unmatched benefits for your lab</strong></h4><div><div data-locator="layoutcontainer-"><div><div id="text-ac6a0745c1" data-locator="text"><ul><li><strong>50% faster data review:</strong> Spend more time on science, less on manual review with the Exception Focused Review functionality</li><li><strong>Efficient batch processing:</strong> Seamlessly manage high sample volumes</li><li><strong>Report results effortlessly:</strong> On your <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?manufacturerItems=5&amp;search=xevo"><strong>Xevo tandem quadrupole family</strong></a></li><li><strong>Ease of use: </strong>Intuitive system interface minimizes training requirements</li><li><strong>Compliance-enabled:</strong> Meet industry regulations such as 21 CFR Part 11 and ISO standards</li><li><strong>Integrated workflows:</strong> Enhance connectivity with streamlined LIMS and mixed-system networking for streamlined operations</li></ul></div></div><div><div id="text-939762e6f1" data-locator="text"><h4><strong>Recommended Use</strong></h4><p>For laboratories performing quantitative analysis that require efficient processing of large sample batches, reduced data review time, and adherence to compliance standards.</p><h3><strong>Advanced visualization</strong></h3><p>Simplify complex data interpretation with advanced visualization tools. Exception Focused Review functionality, lets you set customizable rulesets, quickly spot trends, and instantly identify outliers, enabling faster, data-driven decisions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_waters_connect_for_Quantitation_Advanced_visualization_4f34501146.jpg" alt="Waters: waters_connect for Quantitation: Advanced visualization." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_waters_connect_for_Quantitation_Advanced_visualization_4f34501146.jpg 245w," sizes="100vw" width="1460" height="819"></p><div><div id="text-e5c015d2f3" data-locator="text"><h3 data-locator="modal-title"><strong>Take control of your data</strong></h3><p>Dominate your data with waters_connect for Quantitation. Designed to simplify your lab operations, the dynamic, user-friendly interface ensures effortless navigation, while one-click processing handles even the largest datasets with ease. With centralized access to reports, methods, and sample lists via a secure Oracle Database, you can quickly uncover critical insights and make confident, data-driven decisions faster than ever.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_waters_connect_for_Quantitation_Take_control_of_your_data_96563b6d5a.jpg" alt="Waters: waters_connect for Quantitation: Take control of your data." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_waters_connect_for_Quantitation_Take_control_of_your_data_96563b6d5a.jpg 234w," sizes="100vw" width="1024" height="682"></p><h3><strong>Accelerated analytical workflows, zero compromises</strong></h3><p>Analyzing large sample batches? No problem. The Acquisition Method Editor is an automatic optimization and processing tool designed to save time and reduce transcription error when creating your acquisition and processing method and processing method.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_waters_connect_for_Quantitation_Accelerated_analytical_workflows_zero_compromises_c8ff142bf3.jpg" alt="Waters: waters_connect for Quantitation: Accelerated analytical workflows, zero compromises." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_waters_connect_for_Quantitation_Accelerated_analytical_workflows_zero_compromises_c8ff142bf3.jpg 234w," sizes="100vw" width="1024" height="682"></p><h3><strong>Confidence in compliance</strong></h3><p>Confidently align LC-MS/MS workflows to global regulations and standards, such as 21 CFR part 11 and ISO, using the compliance-enabled waters_connect for Quantitation software. Enhance data integrity through the robust Oracle relational database backbone, which provides robust method version control, traceability, and comprehensive audit trails.&nbsp;Bolster regulatory-driven LC-MS/Ms workflows by combining data integrity with productivity through mixed-system data networks and LIMS connectivity.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_waters_connect_for_Quantitation_Reduce_the_burden_of_data_integrity_with_software_that_facilitates_accurate_traceable_and_secure_records_fb809b433f.jpg" alt="Waters: waters_connect for Quantitation: Reduce the burden of data integrity with software that facilitates accurate, traceable, and secure records." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_waters_connect_for_Quantitation_Reduce_the_burden_of_data_integrity_with_software_that_facilitates_accurate_traceable_and_secure_records_fb809b433f.jpg 234w," sizes="100vw" width="1024" height="682"></p><h3><strong>Advancing PFAS, Pharmaceutical, and Food Safety Analysis Applications</strong></h3><h4><strong>PFAS analysis</strong></h4><p>Achieve precision and productivity in <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=5&amp;search=PFAS"><strong>PFAS testing</strong></a> with software designed for ease of use and compliance. waters_connect for Quantitation helps laboratories meet stringent regulatory requirements while managing complex sample matrices with ease.</p><h4><strong>Pharmaceutical analysis</strong></h4><p>Confidently quantify pharmaceutical analytes from discovery to manufacturing QC with waters_connect for Quantitation. Streamline LC-MS/MS analysis, enhance productivity, and ensure data integrity with compliance-enabled analytical workflows generating the results that support drug development, regulatory submissions and audits.</p><h4><strong>Food and environmental safety</strong></h4><p>Streamline testing for multiresidue <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=5&amp;search=pesticides"><strong>pesticides</strong></a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=5&amp;search=mycotoxin"><strong>mycotoxins</strong></a>, and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=5&amp;search=veterinary+drugs"><strong>veterinary drugs</strong></a> with efficient workflows that maximize throughput without compromising quality. Analyze complex samples quickly and confidently</p><h3><strong>Your Scalable, Compliance-Enabled Solution</strong></h3><p>Built on the waters_connect Informatics Platform, waters_connect for Quantitation empowers analytical labs to achieve faster, more robust outcomes. Its scalable, secure design supports seamless integration with existing Waters software and partner solutions, ensuring future-ready performance.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_waters_connect_for_Quantitation_Core_capabilities_of_the_waters_connect_Platform_22c5991e08.jpg" alt="Waters: waters_connect for Quantitation: Core capabilities of the waters_connect Platform." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_waters_connect_for_Quantitation_Core_capabilities_of_the_waters_connect_Platform_22c5991e08.jpg 153w," sizes="100vw" width="1059" height="1082"><br>&nbsp;</p></div></div></div></div></div></div></div></div>

Waters Empower Chromatography Data System (CDS)

Empower Chromatography Data System (CDS) has been intentionally designed to help you address your most rigorous chromatographic, regulatory, and networking challenges.

<div><div id="title-230ef9ecce" data-locator="title-with-text"><h3><strong>Elevate your chromatography with the universal language of the lab</strong></h3></div></div><div><div id="text-3b75bcd8fb" data-locator="text"><p><strong>Simplifying the way you collect, manage, and report chromatography test results is critical to your lab efficiency. With a suite of flexible functionalities that grows to meet your changing quality, business, and laboratory needs, Empower Chromatography Data System (CDS) has been intentionally designed to help you address your most rigorous chromatographic, regulatory, and networking challenges.</strong></p><p>Empower CDS<strong> </strong>helps your entire laboratory operate more efficiently and securely with the industry's most advanced data acquisition, management, processing, analytics, and reporting capabilities. Easily scalable from a single workstation to an enterprise-wide network, where you can work at the bench, across sites, and even remotely with on-premises or cloud options, Empower CDS provides an environment where your records are traceable, so you always have full control of your most critical asset - your data.</p><div><div id="text-8f4122209c" data-locator="text"><h3><strong>Overview</strong></h3><div data-locator="section-container-body"><div><div><div data-locator="layoutcontainer-"><div><div id="text-07744ba23e" data-locator="text"><ul><li><strong>Store and retrieve chromatography data using an embedded, relational database</strong>; simplifying data retrieval by searching with criteria that is meaningful to you</li><li><strong>Save time, reduce manual integration, and calculate more precise peak integrations</strong> with the ApexTrack algorithm in Empower CDS</li><li><strong>Easily control</strong> Waters high-performance liquid chromatography (HPLC), UHPLC, and UPLC systems, as well as non-Waters HPLC, gas chromatography (GC), photodiode array detector (PDA), and mass spectrometry (MS) systems</li><li><strong>Achieve more enhanced detection capabilities</strong> when using Waters mass detectors or the Waters ACQUITY UPLC PDA Detector</li><li><strong>Grow seamlessly from a single workstation to an enterprise-wide system</strong> with the scalable, modular architecture in Empower CDS, which fits into the most modern and secure high-availability corporate architecture</li><li><strong>Deploy applications</strong> such as dissolution, method validation, integrated chemical structures, and polymer or size exclusion analysis</li></ul></div></div><div><div id="text-a7790dd22b" data-locator="text"><h3><strong>Recommended Use</strong></h3><p>For advanced data acquisition, management, processing, reporting, and distribution of chromatography analysis data.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Empower_Chromatography_Data_System_CDS_Operate_more_efficiently_and_securely_with_the_flexible_capabilities_of_Empower_Chromatography_Data_System_CDS_9625ca12d5.jpg" alt="Waters Empower Chromatography Data System (CDS): Operate more efficiently and securely with the flexible capabilities of Empower Chromatography Data System (CDS)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Empower_Chromatography_Data_System_CDS_Operate_more_efficiently_and_securely_with_the_flexible_capabilities_of_Empower_Chromatography_Data_System_CDS_9625ca12d5.jpg 234w," sizes="100vw" width="1024" height="682"></p><div><div id="text-f39d8a00c0" data-locator="text"><h3 data-locator="modal-title"><strong>Interface options unique to your users</strong></h3><p>Empower Software represents a fundamental shift in <strong>UPLC/UHPLC, HPLC, and LC-MS</strong> system usability. It is equipped with unique interface options designed for your lab, regardless of the task or user's skill level. When users log in, they are presented with the application that provides the appropriate workflow for them. If authorized, users can select another software interface that is suitable for their current task.</p><h5><strong>The three Empower Software applications are:</strong></h5><ul><li><strong>QuickStart</strong>&nbsp;- Collect, process, and report data from a single-window access. Use a wizard to set up methods and run assays. Navigate the entire Empower environment from one screen, including instrument control, view plotted data in real time, view raw data, and access tools to customize and print reports.</li><li><strong>Pro</strong>&nbsp;- Access to all software functions for advanced users, system administrators, or anyone who needs total control.</li><li><strong>Open Access</strong>&nbsp;- Select your method and the number of samples you need to run and get started with the click of a mouse. No chromatography experience is necessary.</li></ul><figure class="image"><img style="aspect-ratio:1024/594;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Empower_Chromatography_Data_System_CDS_The_Empower_Quick_Start_interface_uses_a_navigation_bar_to_guide_you_through_the_software_and_all_tasks_are_carried_out_in_one_window_dfe3ec8bb7.jpg" alt="Waters Empower Chromatography Data System (CDS): The Empower QuickStart interface uses a navigation bar to guide you through the software and all tasks are carried out in one window." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Empower_Chromatography_Data_System_CDS_The_Empower_Quick_Start_interface_uses_a_navigation_bar_to_guide_you_through_the_software_and_all_tasks_are_carried_out_in_one_window_dfe3ec8bb7.jpg 245w," sizes="100vw" width="1024" height="594"></figure><div><div id="text-b65d021cbc" data-locator="text"><h3 data-locator="modal-title"><strong>Ideal for data analysis</strong></h3><h5><strong>Improve data analysis workflows and information sharing across your lab with Empower Software, which helps you achieve:</strong></h5><ul><li><strong>Reliable shoulder peak detection</strong> - ApexTrack effectively detects and integrates shouldered peaks, providing more reliable detection of low-level peaks on noisy or sloping baselines for less manual manipulation and more accurate results.</li><li><strong>Automated custom calculations</strong> - Simply use a wizard to set up your parameters, like averages or % relative standard deviation (RSD). After configuring the calculation once, Empower Software automatically determines the answer when data is processed - no more data transfer, spreadsheet manipulation, or transfer errors.&nbsp;</li><li><strong>Better information sharing </strong>- Easily share information with colleagues worldwide with results and reports stored within the same database. Create combined summary reports across laboratories, instruments, and projects, regardless of where data was acquired or processed. Create PDFs or email reports, distribute methods and information electronically, access information between remote sites, and review and electronically sign off results.</li><li><strong>Fast access to information </strong>- Empower CDS has an embedded relational database, enabling you to store chromatographic results, automatically linking all metadata without manual information tracking or documentation, and better preparing you for review questions or audits. Find data fast and easily with relevant search terms and automatic time stamps of user activity - without a flat file environment, awkward naming conventions, or manual method and result versioning.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Empower_Chromatography_Data_System_CDS_9a791332d9.jpg" alt="Waters Empower Chromatography Data System (CDS): As an example of the breadth of technology that Empower offers, the software can control mass spectrometry (MS) instruments and acquire and process MS data. Spectral information from multiple detection modes can be viewed simultaneously. This enables scientists to easily perform complex analyses with minimal training, increasing lab productivity." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Empower_Chromatography_Data_System_CDS_9a791332d9.jpg 245w," sizes="100vw" width="1024" height="553"></p><div><div id="text-a10f03a0ca" data-locator="text"><h3 data-locator="modal-title"><strong>At-a-glance key chromatography data views to maximize lab resources</strong></h3><p>Empower Laboratory Analytics assembles and displays critical chromatography performance data in easy-to-view, graphical dashboards so you can focus on what’s most important―the interpretation of key information, not its collection.&nbsp;</p><p>Empower Laboratory Analytics offers a range of prebuilt dashboard types, including system summary, system usage, project usage analysis, and methods analysis. These at-a-glance dashboards let you quickly and accurately determine how laboratory resources are being used, without significant manual effort. With these dashboards, you can make evidence-based decisions on which improvements are needed to increase laboratory efficiency.&nbsp;</p><p>With Empower Laboratory Analytics, you can maximize system utilization and better manage resources. You can quickly identify training needs, error messages affecting workflows, and non-robust processing methods. With Empower Software, your lab can better plan for capital expenditures and identify opportunities to shorten run times with UPLC/UHPLC technology.</p></div></div><div><div id="text-497ef11ba3" data-locator="text"><h3 data-locator="modal-title"><strong>Monitor your chromatography system anytime, anywhere</strong></h3><h5><strong>Empower Mobile gives you wireless access to Empower Software from a tablet or smartphone, making it easier to know what’s happening in your laboratory no matter where you are to:</strong></h5><ul><li>Improve laboratory efficiency</li><li>Easily find and share results remotely</li><li>Expedite workflow for signoff on reports</li><li>Lower operating costs for system administration tasks</li><li>Reduce sample and mobile phase waste</li></ul><p>Whether you are an analyst, manager, or system administrator, this easy-to-use application, specially designed to run on mobile devices, gives you the flexibility to work on other priorities, knowing that you can use your mobile device at any time to monitor system status, perform routine system administration tasks, and review and sign off reports.</p><p>Empower Mobile is versatile and has been optimized to work with Apple iPad and iPhone and Android tablet and smartphone devices. Empower Mobile can be installed on your Empower Software network in either workgroup or enterprise configurations, all at no additional cost to you.&nbsp;</p><div><div id="text-8f9e5e7bd5" data-locator="text"><blockquote><h3><strong>Empower 3.9.0 is here</strong></h3><h5><strong>Keep your lab current with the latest Empower Software release built to enhance efficiency, productivity, and compliance readiness so you can drive better scientific and business outcomes across your organization.</strong></h5><h4><strong>With Empower 3.9.0 you can:</strong></h4><ul><li><strong>Reduce risk: </strong>Full Windows® 11 support to keep your data safe with the latest Microsoft® updates.</li><li><strong>Increase efficiency: </strong>Updates in the Review window simplify work for users when workflows require technical controls to reduce the potential for human bias.</li><li><strong>Reduce cost and effort: </strong>New Empower RapidDeploy Software option for LAC/E devices reduces the effort spent planning, preparing, and coordinating LAC/E device upgrades and significantly reduces resource constraints often encountered during upgrades — reducing upgrade costs and minimizing downtime during upgrades so you can&nbsp;keep your operations current.</li></ul><h4><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/nextgen/us/en/services/software-services/software-upgrades/empower-software-upgrade-request.html"><strong>Upgrade to the latest version!</strong></a></h4></blockquote></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div>

Waters MassLynx Mass Spectrometry Software

With development largely driven by input from our extensive user base, MassLynx provides the fundamental platform to acquire, analyze, manage, and share mass spectrometry information.

<h3><strong>Our well-established MassLynx software package delivers market-leading versatility and flexibility for all application areas.</strong>&nbsp;</h3><div><div id="text-f93532e53f" data-locator="text"><p><strong>MassLynx was first pioneered to simplify interaction with mass spectrometry (MS) and has continually improved to meet changing customer needs. The result: a market-leading software solution that allows users to best leverage their MS system and harness real-time intelligence, ensure MS system performance, and more quickly convert sample data into valuable knowledge. With development largely driven by input from our extensive user base, MassLynx provides the fundamental platform to acquire, analyze, manage, and share mass spectrometry information.</strong></p><p>MassLynx Software features a <strong>21 CFR Part 11-compatible security</strong> including <strong>file encryption, audit log</strong>, and <strong>secure archival/retrieval technology</strong> with flexible security policies to meet your lab requirements. Waters also offers excellent <a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/nav.htm?cid=1000349" data-di-id="di-id-5aca7e0f-b105e917"><strong>service and suppor</strong>t</a> to get the system up and running – for on-time results without the stress.</p><h2><strong>MassLynx Quantitation Applications</strong></h2><div><div id="title-eb2a5ffa29" data-locator="title-with-text"><h4><strong>Streamline quantitative data acquisition and processing</strong></h4></div></div><div><div id="text-baefaeaec6" data-locator="text"><p>The rate determining step in many analyses is not data acquisition, but the speed your data can be converted into useful information.</p><p>To assist you with both application-specific data acquisition and the transformation of data into usable results, MassLynx for quantitation offers a range of <strong>application managers</strong> with specialized data processing functionalities, which eliminates the need for complex time-consuming manual data review, increasing your productivity.</p><p>The functionality of <strong>MassLynx application managers</strong> has been guided by our customers and optimized for your data analysis and processing requirements.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Mass_Lynx_Quantitation_Applications_b5a284165b.jpg" alt="Waters: MassLynx Quantitation Applications." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Mass_Lynx_Quantitation_Applications_b5a284165b.jpg 245w," sizes="100vw" width="1428" height="625"></p><h3><strong>Overview</strong></h3><div data-locator="section-container-body"><div><div><div data-locator="layoutcontainer-"><div><div id="text-4b29255c4a" data-locator="text"><ul><li>A tried and well-established software package, MassLynx delivers the versatility and flexibility you require for all of your quantitation applications</li><li>MassLynx security system provides compliance ready tools to meet GxP and 21 CFR Part 11 regulations and TargetLynx XS delivers the information necessary for regulatory compliance</li><li>Inlet method editor and MS method editor allow the user to intuitively build LC-MS/MS methods for quantitative data acquisition</li><li>Auto-dwell for MRM dwell times makes it easy to optimize MRM methods to maximize points per peak and get the best quantitative performance</li><li>IntelliStart guarantees maximum system performance and usability for reproducible results</li></ul></div></div><div><div id="text-adc477d45b" data-locator="text"><h3><strong>Recommended Use</strong></h3><p>MassLynx quantitation applications streamline quantitative data acquisition and processing.</p><div><div id="title-be02784995" data-locator="title-with-text"><h2><strong>MassLynx Mass Detection Applications</strong></h2></div></div><div><div id="title-d5b484d548" data-locator="title-with-text"><h4><strong>Intuitively interact with your mass detection system with MassLynx</strong></h4></div></div><div><div id="text-0fc50dc8ea" data-locator="text"><p>The challenges of mass spectrometry (MS) analyses are often compounded by the use of several types of analytical instruments and high personnel turnover. To maintain and even increase laboratory productivity and efficiency, MassLynx Software simplifies interaction with your mass detection system. MassLynx enables users with a wide range of expertise to easily acquire high-quality mass spectral data with minimal training.</p><p>MassLynx application managers enable you to focus the power of MS on your laboratory's tasks.</p><h3><strong>Overview</strong></h3><div data-locator="section-container-body"><div><div><div data-locator="layoutcontainer-"><div><div id="text-fb3c291dd5" data-locator="text"><ul><li>MassLynx delivers the versatility and flexibility you require for all of your mass detection applications</li><li>Easy-to-use application managers enable you to simply transform mass spectral data into usable results</li><li>Intuitive setup and interaction with the mass detector guarantees optimal performance and reproducible results</li><li>Routinely using the same intuitive software provides familiarity and simplicity for more efficient workflows</li><li>Inlet method editor and MS method editor allow the user to intuitively build LC-MS methods for qualitative and quantitative data acquisition</li></ul></div></div><div><div id="text-4b89cfd965" data-locator="text"><h3><strong>Recommended Use</strong></h3><p>Masslynx Application Managers enable laboratories to confidently produce high quality results without complexity.</p><p><img style="aspect-ratio:1086/319;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Mass_Lynx_Mass_Detection_Applications_f261c2afd6.jpg" alt="Waters: MassLynx Mass Detection Applications." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Mass_Lynx_Mass_Detection_Applications_f261c2afd6.jpg 245w," sizes="100vw" width="1086" height="319"></p><div><div id="title-67851c4cd4" data-locator="title-with-text"><h2><strong>MassLynx High-Resolution Applications&nbsp;</strong></h2></div></div><div><div id="title-1688118c0d" data-locator="title-with-text"><h4><strong>Fully characterize your samples with Waters high-resolution mass spectrometry</strong></h4></div></div><div><div id="text-fc7cb897e2" data-locator="text"><p>As the power of high-resolution mass spectrometry (HRMS) allows today’s labs to obtain high quality data, the challenge is then to confidently identify and characterize components of interest.</p><p>Waters MassLynx Applications enable your lab to efficiently process and interpret HRMS data sets – whether your samples are biological, chemical, environmental, or pharmaceutical, we have a solution that meet your needs, whether it's based on gas chromatography (GC-MS) or liquid chromatography mass spectrometry (LC-MS).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Mass_Lynx_High_Resolution_Applications_9811f2e6b9.jpg" alt="Waters: MassLynx High-Resolution Applications." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Mass_Lynx_High_Resolution_Applications_9811f2e6b9.jpg 245w," sizes="100vw" width="1422" height="594"></p><h3><strong>Overview</strong></h3><div data-locator="section-container-body"><div><div><div data-locator="layoutcontainer-"><div><div id="text-b16ee61766" data-locator="text"><ul><li>Rapidly identify metabolites with MetaboLynx XS</li><li>Achieve confident structural elucidation with MassFragment</li><li>Automate the processing of LC-MS, GC-MS, LC-MS/MS, or GC-MS/MS data with ChromaLynx</li><li>Meet quantitative and qualitative proteomics research requirements with ProteinLynx Global Server (PLGS)</li></ul></div></div><div><div id="text-1dee9a4274" data-locator="text"><h3><strong>Recommended Use&nbsp;</strong></h3><p>For identifying and characterizing data acquired using high-resolution mass spectrometry.</p></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div>

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