<p><strong>Ion exchange (IEX) chromatography is an important separation technique that allows the separation of various ionizable molecules on the basis of the charge. With its unique combination of features, ion exchange chromatography stands out as an efficient chromatographic method because it:</strong></p><ul><li>Handles large sample volumes with ease.</li><li>Offers broad applications, particularly for enzymes and proteins.</li><li>Provides powerful resolving capabilities.</li><li>Supports automation for enhanced efficiency.</li><li>Features a simple scale-up mechanism.</li><li>Balances performance and affordability with moderate costs.</li></ul><h3><strong>Ion Exchange Chromatography’s Mechanism of Action</strong></h3><p>The core principle of ion exchange chromatography is based on the reversible exchange of ions between a mobile phase (the liquid solution containing the sample) and a stationary phase (the solid support with charged groups). The stationary phase can be either:</p><ul><li><strong>Cation Exchange:</strong> Contains negatively charged groups that attract and bind positively charged ions (cations).</li><li><strong>Anion Exchange:</strong> Contains positively charged groups that attract and bind negatively charged ions (anions).</li></ul><p>When a sample is introduced into the column, ions in the sample compete with counterions present on the stationary phase for binding sites. The strength of this interaction depends on several factors, including the charge density and size of the ions involved. Ions with higher charge densities typically bind more strongly to the stationary phase than those with lower charge densities.</p><p>This technique is used in various pharmaceutical industries due to its ability to separate various types of desired charged molecules. It is widely used for the purification of enzymes, amino acids, proteins, antibodies, simpler carbohydrates, organic compounds, and nucleic acids.</p><blockquote><p>Explore the <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/hplc-ion-exchange"><strong>ion exchange technique</strong></a> to achieve effective separation in chromatography applications.</p></blockquote><h3><strong>Ion Exchange Methodology – How does it work?</strong></h3><p>The process of ion exchange chromatography can be broken down into several stages:</p><ul><li><strong>Equilibration:</strong> The column is equilibrated with a buffer solution that matches the pH required for optimal binding of the target analytes.</li><li><strong>Sample Loading:</strong> The sample is introduced into the column, where its charged components interact with the oppositely charged sites on the stationary phase. This interaction influences the extent to which each molecule is retained.</li><li><strong>Washing:</strong> A washing buffer is used to remove unbound or weakly bound impurities from the column.</li><li><strong>Elution:</strong> Bound molecules are eluted using a gradient of increasing salt concentration or by adjusting the pH, which alters the charge on the analytes and facilitates their release from the stationary phase.</li><li><strong>Collection and Analysis:</strong> Eluted fractions are collected and analyzed using various methods, such as UV spectroscopy or mass spectrometry, to identify and quantify the separated components.</li></ul><h3><strong>Applications of Ion Exchange Chromatography</strong></h3><ul><li><strong>Protein and Enzyme Purification:</strong> Widely used to isolate and purify proteins, enzymes, and other biomolecules based on their charge.</li><li><strong>Nucleic Acid Separation:</strong> Essential for separating and purifying DNA, RNA, and oligonucleotides.</li><li><strong>Amino Acid Analysis:</strong> Utilized for the analysis and quantification of amino acids in complex samples.</li><li><strong>Pharmaceutical Industry:</strong> Applied in drug formulation, quality control, and purification of biopharmaceuticals.</li><li><strong>Water Treatment:</strong> Used to remove unwanted ions, such as hardness-causing calcium and magnesium, from water.</li><li><strong>Food and Beverage Industry:</strong> Assists in purifying food additives, removing contaminants, and enhancing product quality.</li><li><strong>Metabolite Analysis:</strong> Facilitates the identification and separation of small charged metabolites.</li></ul><h3><strong>Key Advantages of Ion Exchange Chromatography</strong></h3><ul><li>One of the primary benefits is its ability to separate molecules based on their charge, which is a fundamental property of many biomolecules such as proteins, peptides, and nucleic acids.</li><li>Another significant advantage is its versatility. Ion exchange chromatography can operate under various conditions, including changes in pH, salt concentration, and temperature, allowing for the separation of a broad range of molecules. This flexibility enhances its applicability in different research fields, from pharmaceuticals to environmental analysis.</li><li>Additionally, ion exchange chromatography offers high resolution and reproducibility, ensuring that desired molecules are purified with excellent yield. The technique is also scalable, making it suitable for both small-scale laboratory work and large-scale industrial applications.</li></ul><h3><strong>Limitations of Ion Exchange Chromatography</strong></h3><ul><li>One significant limitation is its sensitivity to pH and ionic strength. The pH of the mobile phase must be carefully controlled, as changes in pH can affect the ionization state of both the analytes and the stationary phase, leading to incomplete separation or poor resolution. Similarly, the ionic strength of the elution buffer must be optimized; high salt concentrations are often necessary for eluting strongly bound analytes, which can be challenging for sensitive compounds that may precipitate or lose activity under such conditions.</li><li>Another limitation is the potential for sample degradation. High salt concentrations and extreme pH values required for elution can denature proteins or destabilize sensitive biomolecules. Additionally, the capacity of ion exchange columns is finite, meaning that the sample load must be carefully managed. Overloading the column can lead to poor separation and reduced resolution.</li><li>Ion exchange chromatography is also relatively time-consuming, as the elution process often involves gradual gradients of salt or pH, which can extend run times. Moreover, the technique is not suitable for all types of analytes, particularly non-ionic compounds or those that do not exhibit significant charge differences under the conditions used.</li></ul>

Principles of Ion Exchange Chromatography: A Comprehensive Guide

<h3><strong>What Gives Ginger Its Characteristic Kick?</strong></h3><p><strong>Fresh ginger is bright, aromatic, and sharply spicy, while dried or heated ginger develops a warmer and more intense character. Behind these sensory differences lies a group of bioactive compounds known as gingerols and shogaols. In this study, HPLC was used to compare their presence in fresh ginger, ginger tea, and several commercially available ginger shots.</strong></p><h4><strong>Gingerols and Shogaols: The Chemistry Behind Ginger</strong></h4><p>Ginger (<i>Zingiber officinale</i>) is widely valued both as a food ingredient and for its bioactive constituents. Among these, <strong>[6]-gingerol</strong> is the predominant and most extensively studied pungent compound in fresh ginger. Smaller amounts of <strong>[8]-gingerol</strong> and <strong>[10]-gingerol</strong> are also naturally present.</p><p>Processing changes this chemical profile. When ginger is heated or dried, gingerols can undergo dehydration and form a related group of compounds known as <strong>shogaols</strong>. One of these, <strong>[6]-shogaol</strong>, is associated with strong antioxidant activity and has also been investigated for its anti-inflammatory properties.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_Structure_of_6_gingerol_a737949416.png" alt="KNAUER: Figure 1: Structure of [6]-gingerol (drawn with MarvinSketch 6.0.1)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_Structure_of_6_gingerol_a737949416.png 245w," sizes="100vw" width="525" height="170"></p><h4><strong>Why Is Ginger So Popular?</strong></h4><p>Ginger is commonly associated with digestive support and is frequently used to help alleviate nausea, including motion sickness and mild gastrointestinal discomfort. Its constituents have also been studied in connection with inflammatory processes, muscle and joint discomfort, and broader physiological effects.</p><p>The chemical composition of ginger products, however, can vary considerably depending on the raw material, formulation, processing conditions, and extraction method. This makes instrumental analysis useful for examining what different ginger products actually contain.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Ginger_picture_generated_by_Open_AI_50c689581b.png" alt="KNAUER: Figure 2: Ginger picture generated with DALL-E (OpenAI; GPT-4o)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Ginger_picture_generated_by_Open_AI_50c689581b.png 156w," sizes="100vw" width="454" height="454"></p><blockquote><p><i><strong>“And I had but one penny in the world, thou shouldst have it to buy gingerbread.”</strong></i><br><i>– William Shakespeare, Love’s Labour’s Lost</i></p></blockquote><h4><strong>Taking a Closer Look at I.N.G.W.E.R.</strong></h4><p>The study compared several commercial ginger shots with <strong>fresh ginger root and ginger tea</strong>.</p><p>The liquid ginger products were first centrifuged and the supernatant removed. The remaining solid material was extracted with <strong>methanol/water 50:50 (v/v)</strong> and centrifuged again. The resulting extract was passed through a <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2380"><strong>0.22 µm nylon syringe filter</strong></a> before injection into the <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/products?category=39&amp;manufacturerItems=503"><strong>HPLC system</strong></a>.</p><p>Chromatographic separation was performed under reversed-phase conditions using a <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1635"><strong>KNAUER Eurospher II C18H column</strong></a>:</p><ul><li>Column dimensions: <strong>150 × 3 mm</strong></li><li>Particle size: <strong>3 µm</strong></li><li>Order number: <strong>15CE185E2G</strong></li><li>Flow rate: <strong>0.8 mL/min</strong></li></ul><p>The gingerol concentration was <strong>not determined quantitatively</strong>. Instead, chromatographic signal heights were compared with those obtained from freshly extracted ginger root. Direct comparison between commercial products also needs to take into account that the individual shots contained different proportions of ginger.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Table_1_Percentage_of_ginger_in_shot_samples_according_to_their_list_of_ingredients_1a064c0399.jpg" alt="KNAUER: Table 1 Percentage of ginger in shot samples according to their list of ingredients" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Table_1_Percentage_of_ginger_in_shot_samples_according_to_their_list_of_ingredients_1a064c0399.jpg 210w," sizes="100vw" width="1107" height="820"></p><h4><strong>Ginger Content of the Tested Shots</strong></h4><h5><strong>Fresh Ginger and Ginger Tea</strong></h5><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Left_Chromatogram_of_fresh_ginger_root_4d6339e54d.jpg" alt="KNAUER: Left: Chromatogram of fresh ginger root, 5 g sample was extracted with MeOH:H₂O; 1 – [6]-gingerol,  2 – [8]-gingerol, 3 – [10]-gingerol. Right: Chromatogram of YogiTea ginger lemon, tea bag (1.8 g) was extracted with 200 ml hot water;  1 – [6]-gingerol" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Left_Chromatogram_of_fresh_ginger_root_4d6339e54d.jpg 245w," sizes="100vw" width="1537" height="485"></p><p>Fresh ginger provided the clearest representation of the characteristic gingerols. In contrast, only a small [6]-gingerol signal was observed in the tea extract. One important difference is that the tea was prepared using hot water rather than the methanol/water extraction applied to the other samples.</p><h5><strong>Classic and Pomegranate Ginger Shots</strong></h5><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Left_Chromatogram_of_classic_ginger_shot_b9de81bb02.jpg" alt="KNAUER: Left: Chromatogram of classic ginger shot, 1 -  [6]-gingerol, 2 - [8]-gingerol, 3 - [6]-shogaol,  4 - [10]-gingerol. Right: Chromatogram of  pomegranate ginger shot, 1 -  [6]-gingerol, 2 - [8]-gingerol, 3 - [6]-shogaol,  4 - [10]-gingerol" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Left_Chromatogram_of_classic_ginger_shot_b9de81bb02.jpg 245w," sizes="100vw" width="1522" height="475"></p><p>Both products showed a comparatively rich ginger-derived profile. In addition to <strong>[6]-gingerol</strong>, the chromatograms contained <strong>[8]-gingerol, [10]-gingerol, and [6]-shogaol</strong>.</p><p>Among the commercial products examined, the <strong>classic and pomegranate shots showed the strongest [6]-gingerol signals</strong>.</p><h5><strong>Turmeric and Fruit Shots</strong></h5><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Left_Chromatogram_of_Kurkuma_ginger_shot_b8887746f8.jpg" alt="KNAUER: Left: Chromatogram of Kurkuma ginger shot, 1 -  [6]-gingerol. Right: Chromatogram of  fruit shot, 1 -  [6]-gingerol" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Left_Chromatogram_of_Kurkuma_ginger_shot_b8887746f8.jpg 245w," sizes="100vw" width="1527" height="477"></p><p>In these two samples, <strong>[6]-gingerol</strong> was the principal ginger-related compound identified. The fruit shot produced the lowest [6]-gingerol signal of all the tested ginger shots, which is consistent with its comparatively low declared ginger content of <strong>2%</strong>.</p><h5><strong>Vitamin Acai and Pineapple Ginger Shots</strong></h5><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Left_Chromatogram_of_Vitamin_Acai_shot_459cc69719.jpg" alt="KNAUER: Left: Chromatogram of Vitamin Acai shot, 1 -  [6]-gingerol,  2 - [8]-gingerol, 3 - [6]-shogaol,  4 - [10]-gingerol. Right: Chromatogram of  pineapple ginger shot, 1 -  [6]-gingerol, 2 - [8]-gingerol, 3 - [6]-shogaol,  4 - [10]-gingerol" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Left_Chromatogram_of_Vitamin_Acai_shot_459cc69719.jpg 245w," sizes="100vw" width="1510" height="465"></p><p>The Vitamin Acai and pineapple products showed a broader profile similar to the classic and pomegranate shots. <strong>[6]-gingerol, [8]-gingerol, [10]-gingerol, and [6]-shogaol</strong> were detected in both samples.</p><h4><strong>What Did the HPLC Analysis Reveal?</strong></h4><p><strong>[6]-Gingerol was detected in every sample analyzed.</strong> The freshly extracted ginger root gave the strongest reference response, while among the commercial ginger shots, the classic and pomegranate variants showed the highest [6]-gingerol signals.</p><p>The <strong>fruit shot contained the lowest amount based on relative signal height</strong>, while the hot-water ginger tea extract produced only a small gingerol response.</p><p>A broader range of ginger-derived compounds was observed in the <strong>classic, pomegranate, pineapple, and Vitamin Acai shots</strong>, where [8]-gingerol, [10]-gingerol, and [6]-shogaol were detected alongside [6]-gingerol.</p><p>Because the experiment was designed as a comparative rather than quantitative analysis, the results should be interpreted as differences in chromatographic response rather than absolute gingerol concentrations. Nevertheless, the experiment demonstrates how straightforward reversed-phase HPLC can be used to compare the characteristic bioactive compounds present in fresh ginger and processed ginger products.</p><p>Whether fresh, brewed, or packed into a concentrated shot, ginger clearly offers more analytical complexity than its distinctive spicy taste might suggest.</p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/I_N_G_W_E_R_Analytica_254b7c26e3.pdf"><strong>Download application note I.N.G.W.E.R. – Instrumental Analysis of Nutritional Ginger Wellness Extracts here</strong></a></p></blockquote>

I.N.G.W.E.R. – Instrumental Analysis of Nutritional Ginger Wellness Extracts & Residues

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 14th September 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you application notes by <strong>Agilent Technologies </strong>and <strong>Waters Corporation </strong>and posters by <strong>Shimadzu / AOAC </strong>and<strong> Thermo Fisher Scientific / Metabolomics!</strong></p><h3><strong>1. Agilent Technologies: Improved Analysis of Intact Proteins and Peptides by Reversed Phase HPLC Using the Altura Ultra Inert PLRP-S 1000 Å Column</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_altura_ultrainert_column_HPLC_analysis_5994_9322en_agilent_2b3a542647.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Intact protein analysis by reversed-phase HPLC (RP-LC) plays a vital role in quality control for determining the purity of biotherapeutic proteins and improving the identification and quantification of critical quality attributes (CQAs). When coupled to mass spectrometric detection, it allows several things. First, an accurate molecular weight to be determined; second, the identification of variants containing post‑translational modifications (PTMs) and proteoforms; and third, the antibody-to-drug ratio in the case of antibody drug conjugates (ADCs).<sup>1</sup>&nbsp;</p><p>While many RP columns have a silica-based stationary phase to enable broader functionalization, residual acidic silanol groups can interact with basic residues in peptides, proteins, and other biomolecules, resulting in significant peak tailing and loss of chromatographic performance. Polystyrene/divinyl benzene (PS/DVB)-based stationary phases do not contain unintentional adsorption sites. Additionally, larger pore sizes have demonstrated superior chromatographic efficiency through less diffusion restriction into the pore volume and reduction of size exclusion mechanisms, degrading the RP-based selectivity.<sup>2</sup>&nbsp;</p><p>Wide-pore columns like the PLRP-S 1000Å provide an exceptional performance with biomolecules, particularly when combined with MS-compatible mobile phase conditions such as formic acid.&nbsp;</p><p>Even so, some undesirable interactions may be observed when using stainless-steel column hardware. Molecules that possess multiple negative charges (including oligonucleotides, phosphorylated peptides and proteins, and proteins with low isoelectric point in general) may interact with exposed metal ions in stainless-steel column hardware. This can result in the need to "deactivate" new columns by making multiple injections of sample until reproducible results are obtained.&nbsp;</p><p>This application note demonstrates how the new <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=10&amp;search=Altura+Ultra+Inert"><strong>Altura Ultra Inert </strong></a>PLRP-S 1000 Å column serves as an effective high-performance workhorse in the analysis of large, intact biomolecules. Developed to effectively block nonspecific adsorption during separations, Ultra Inert technology reduced passivation time 15-fold and delivered ~ 10% greater recovery for mAbs and ADCs and up to 100% for smaller biomolecules like GLP-1s and small globular proteins.</p><h4><strong>Experimental</strong></h4><h5><strong>Instrumentation&nbsp;</strong></h5><ul><li>Agilent 1290 Multicolumn thermostat (G7116B)&nbsp;</li><li>Agilent 1290 Bio multisampler (G7137A)&nbsp;</li><li>Agilent 1290 Bio high-speed pump (G7132A)&nbsp;</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=10&amp;search=6530"><strong>Agilent 6530 Q-TOF MS system</strong></a>&nbsp;</li></ul><h5><strong>Software and data processing&nbsp;</strong></h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=10&amp;search=MassHunter+Workstation"><strong>Agilent MassHunter Workstation, version 10.1&nbsp;</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=10&amp;search=MassHunter+Qualitative+Analysis"><strong>Agilent MassHunter Qualitative Analysis software, version&nbsp;B.07&nbsp;</strong></a></li><li>Agilent DAR calculator, version B01.01</li></ul><h4><strong>Conclusion&nbsp;</strong></h4><p>Intact mass analysis is a critical methodology to determine the purity of biotherapeutics, but due to the chemical complexity of these analytes, nonspecific adsorption from both silica stationary phase and metal column housing can be highly deleterious to separation performance. The Agilent Altura PLRP-S 1000 Å provides a highly robust solution combining the chemical inertness of polystyrene/divinyl benzene stationary phase with the ultra‑inert Altura hardware to dramatically reduce deleterious peak tailing and increase recovery for biotherapeutics. Herein, we see that analyte recovery for both mAbs, ADCs, GLP-1s, and globular proteins are improved versus stainless-steel, while the Altura PLRP-S column is capable of monitoring both quality critical PTMs such as loss of drug payload as well as accurate quantification of ADC DAR.</p><h3><strong>2. Shimadzu / AOAC: Ambient Ionization Mass Spectrometric Detection of Aroma Released During Gummy Chewing</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/aoac_2026_47_2_34c41f762f.pdf"><strong>Full PDF for download</strong></a></li></ul><p>In recent years, there has been growing interest in evaluating flavor using analytical instrumentation, and retronasal aroma during eating is important. In this study, we proposed an analytical method for measuring retronasal aroma during eating using a single quadrupole mass spectrometer (<a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=2050"><strong>LCMS-2050</strong></a>) equipped with an ambient ion source (Dark Current Discharge Ionization : DCDI) without pretreatment.</p><h4><strong>Conclusion</strong></h4><p>We developed a simple method for evaluating retronasal aroma using a single quadrupole mass spectrometer with an ambient ionization source. Ambient ionization mass spectrometry (DCDI LCMS-2050) can rapidly measure retronasal aroma during eating. Time-resolved visualization reveals texture-dependent differences in aroma release dynamics among grape-flavored gummies. It is also expected to lead to the visualization of the marriage between food and alcohol. This approach may facilitate objective evaluation of flavor release during consumption and support product formulation and sensory science.</p><h3><strong>3. Thermo Fisher Scientific / Metabolomics: Orbitrap Tribrid Real-Time similarity searching of mzCloud library for guided unknown characterization of tea metabolites</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/po_004849_lsms_rtls_tribrid_apex_small_molecule_metabolomics_po004849_na_en_d0d3156065.pdf"><strong>Full PDF for download</strong></a></li></ul><p>In metabolomics LC-MS data is processed to generate a list of detected compounds with a level of confidence in the identity of each compound based on reference data. This reference data may be spectral data and/or retention time information stored in a library or in silico information generated based on fragmentation rules. These annotated compounds are then used to evaluate biological changes between normal and disease states. One of the major hurdles between collected data and coming to reliable conclusions about the biology is the limited number of standards available to build these libraries. It is common to have hundreds to thousands of compounds detected in a data set that have no library match because there is no commercial standard for that unknown. Instead, there is just an elemental formula and matches from a structure database. These structures can be compared using in silico fragmentation techniques to determine which isomers plausibly match the data. MSn data can help reduce the list of possible matches, but without guidance, ddMS3 may not consistently collect data on the most relevant MS2 fragments to accomplish this.&nbsp;</p><p>In this work we use Real-Time Library Search (RTLS) in a ddMS3 method to help guide the instrument in real time to collect the most relevant data for confirming structural matches.</p><h4><strong>Materials and methods</strong></h4><h5><strong>Test method(s)&nbsp;</strong></h5><p>1 μL aliquots were analyzed using a <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/products?category=48&amp;manufacturerItems=3&amp;search=gold"><strong>Thermo Scientific Hypersil GOLD column</strong></a> on a <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/744"><strong>Thermo Scientific Vanquish Horizon UHPLC</strong></a><strong> </strong>coupled with a <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/561"><strong>Thermo Scientific Orbitrap IQ-X Tribrid mass spectrometer</strong></a> and <strong>Thermo Scientific Orbitrap Tribrid Apex Small Molecule mass spectrometer</strong>. RTLS was run in similarity mode using the offline version of <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1764"><strong>Thermo Scientific mzCloud library</strong></a>.&nbsp;</p><h5><strong>Data analysis&nbsp;</strong></h5><p>Data was processed using <strong>Thermo Scientific Freestyle and </strong><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1759"><strong>Compound Discoverer software</strong></a><strong>.</strong></p><h4><strong>Conclusions&nbsp;</strong></h4><p>Tea extracts were analyzed using RTLS to find the most similar compound in the offline version of the mzCloud library and trigger MS3 on the highest m/z matching and non-matching fragments. This provided additional information to confirm similarity and provide insight on shared sub-structures to the library compound as well as additional information on the portions of the structure that were unique to the experimental compound.</p><h3><strong>4. Waters Corporation: The Quantification of Glucagon-like Peptide Receptor Agonists (GLP-1 RAs) in Plasma Extracts Using Capillary UHPLC-MS/MS</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/720009537_en_0068921516.pdf"><strong>Full PDF for download</strong></a></li></ul><div><div><section><div><div><div><div><div><div><h5><strong>Benefits&nbsp;</strong></h5><ul><li>10-fold improvement in sensitivity&nbsp;</li><li>Robust methodology for &gt;500 injections&nbsp;</li><li>Reduced solvent usage&nbsp;</li><li>No need for complex nanospray MS source</li></ul><p>Obesity represents, perhaps, the largest healthcare challenge, affecting approximately 1.3 billion people globally, contributing to a diverse range of chronic diseases e.g., metabolic, cardiovascular, muscular skeletal, and multiple cancers.1 The rapid growth of obesity and associated global healthcare costs means there is a critical need for safe effective treatments.2 GLP-1 RA initially developed for type 2 diabetes have shown clinical efficacy as weight loss medicines.2 These drugs mimic the action of gut hormones which regulate energy metabolism and appetite by interacting with the hypothalamus, brain stem etc., resulting in weight loss. The success of these GLP-1 RAs is associated with their on-target efficacy and long duration of action,3 e.g., half-life of semaglutide t½ = 160–180 hours3 cf. 1–2 minutes for human GLP-1.4 The long half-life of these GLP-1 RAs results in low, sub ng/mL plasma levels 48–72 hours after dosing, which requires a high sensitivity bioanalytical assay to accurately quantify the plasma concentrations5 and define the PK elimination phase.&nbsp;</p><p>Tandem quadrupole electrospray ionization (ESI) mass spectrometry, operated in multiple reaction monitoring (MRM) mode, coupled with 2.1 mm ID ultra-high performance liquid chromatography (UHPLC) is the dominant technology platform for quantitative bioanalysis. As next generation GIP/GLP-1 RAs promise to be more potent, resulting in lower dosing levels and plasma levels, there is a need for more sensitive analytical methodologies to support drug metabolism and pharmacokinetics (DMPK) studies. Capillary/nano LC-MS/MS have been extensively employed in proteomics to increase analytical sensitivity and enable small volume analysis.5 This sensitivity increase is derived from a combination of the superior ionization efficiency at low flow rates and analyte concentration due to the smaller column geometry.6 This application note illustrates the use of capillary (300 µm) UHPLC-MS/MS (MRM) to affect 10-fold improvement in GLP-1-RAs peak response in human plasma extracts, and detection limits as low as 20 pg/mL for tirzepatide.</p><h4><strong>Experimental</strong></h4><p>Capillary chromatography was performed on an <strong>ACQUITY™ M-class Chromatography System</strong> connected to a <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/195"><strong>Xevo™ TQ-XS Mass Spectrometer</strong></a> (Waters Corporation, Wilmslow, UK) equipped with a microflow probe. Capillary UHPLC separations were performed on an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1952"><strong>ACQUITY Premier Peptide CSH C18</strong></a>, 300 Å, 1.7 µm, 300 µm x 50 mm Chromatography Column.</p><p>MS analysis was performed at unit mass resolution using positive ESI at a capillary voltage of 3.0 kV, with a cone voltage of 32 V. The cone gas flow, desolvation gas flow, and desolvation gas temperature were 50 L/h, 300 L/h and 600 °C, respectively. The data were analyzed using <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1544"><strong>waters_connect™ Software</strong></a><strong>,</strong> and MRM transitions used to monitored for the MS/MS detection of liraglutide, exenatide, semaglutide and tirzepatide were as follows: liraglutide 938.7 → 1128.4, exenatide 838 → 396, semaglutide 1029.2 → 1238.1, and tirzepatide 1204.2 → 396.2. Data analysis was performed using <strong>MS Quan Software.</strong></p><h4><strong>Conclusion</strong></h4><p>Bioanalysis plays a critical role in the drug discovery and development processes providing data to support DMPK, safety assessment, pharmacokinetics/pharmacodynamics (PK/PD), and clinical development studies. The development of new, more potent medicines, innovating delivery platforms, micro sampling, and new approach methodologies (e.g., organ on a chip) all require greater levels of analytical sensitivity. Capillary scale UHPLCMS/MS offers the potential to improve bioanalytical detection limits up to 10-fold, without the need for long analysis times and complex operation associated with nano spray LC-MS/MS. Here, the application of capillary (300 µm ID) scale chromatography for the detection of GLP-1 RAs in human plasma extracts at levels as low as 20 pg/mL with robustness over 500 injections was demonstrated.</p></div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 38, 2026

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

KNAUER AZURA 1240 bar UHPLC System

High-end solution for most challenging tasks. Ideally suited for quality control tasks with high sample throughput.

<p><strong>High-end solution for most challenging tasks.&nbsp;Ideally suited for quality control tasks with high sample throughput. This solution is recommended whenever lowest detection limits, highest analysis speed and resolution or most accurate flow delivery is needed.</strong></p><div><div><ul><li>Developed with innovative technology and decades of continuous improvements <strong>the AZURA®&nbsp;UHPLC 1240 bar systems enter&nbsp;a new level of performance and durability</strong></li><li><strong>Covers ultra high pressure applications</strong> due&nbsp;to&nbsp;working conditions in the range of&nbsp;<strong>0.1–5.0&nbsp;ml/min at up to 1240&nbsp;bar&nbsp;</strong></li><li>This system is made for&nbsp;high resolution <strong>UHPLC columns packed with smallest particles &lt; 2 µm</strong> or <strong>core shell materials</strong>, while any other HPLC or UHPLC column can also be used&nbsp;</li><li><strong>Perfect partner for MS/MS coupling</strong> due to very precise and accurate delivery also in the low flow rate range</li><li>Typical applications include food, pharmaceutical and environmental analyses with high throughput</li></ul></div></div><div><div><p>Choose this system whenever highest performance and speed is needed!</p><p><strong>If your applications do not require such a high back pressure and resolution, then you should take a look at the KNAUER AZURA HPLC systems. These can be operated at up to 862 bar.</strong></p></div></div><h3><strong>Specifications</strong></h3><ul><li><strong>Back pressure range:</strong> 1240 bar max.</li><li><strong>Flow rate range:</strong> 0.001–5 ml/min</li><li><strong>Available pump systems:</strong> Binary HPG</li><li><strong>Special pump features:</strong> Active pulsation compensation, RFID for automatic pump head detection, Integrated pressure sensor, Leak management, Piston seal wash, Purge valve (automatic)</li><li><strong>Injection options:</strong> Autosampler, Liquid handler</li><li><strong>Injection volume range:</strong> 0.1 µl–5000 µl depending on sample loop</li><li><strong>Sample capacity:</strong> 108 standard HPLC vials (1.5 ml) or up to 768 positions when equipped with microtiter plates, with liquid handler up to 390 HPLC vials</li><li><strong>Column thermostatting range: </strong>5–85 °C</li><li><strong>Eluent preheating:</strong> Optional cartridge for eluent pre-heating</li><li><strong>Column switching options:</strong> Optional switching between up to 8 columns</li><li><strong>Choice of detectors:</strong> DAD 190–700 nm, DAD 190–1000 nm, RID, UVD, MWD, FLD, ELSD, MS</li><li><strong>Choice of software packages:</strong> ClarityChrom® CDS, OpenLAB® CDS EZChrom Edition, Mobile Control, Chromeleon™ 7.2</li></ul><h3><strong>System components</strong></h3><div><div><p>An AZURA UHPLC system adapts to your application requirements. The highly modular system design allows you to choose the best suited components for your specific task.</p></div></div><h4><strong>Eluent delivery</strong></h4><h5><strong>Binary high-pressure gradient (HPG) pump system</strong></h5><p>The new <strong>AZURA®&nbsp;P 8.1L UHPLC pump</strong> is specially designed for ultra-fast and high-resolution applications.&nbsp;It features a 2-channel internal degassing unit, a 2x2 channel solvent selection valve, and a low dwell-volume eluent mixer.</p><p>The adaptive pulsation compensation of the pump is independent of flow rate, backpressure, and eluent type through real-time eluent compressibility monitoring and variable piston stroke. Together with the ultra-precise piston movement thanks to KNAUER’s proprietary advanced piston drive technology, this results in outstanding flow reproducibility at any working conditions.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_KNAUER_AZURA_1240_bar_UHPLC_System_Binary_high_pressure_gradient_HPG_pump_system_b94a8018c7.jpg" alt="Knauer: KNAUER AZURA 1240 bar UHPLC System - Binary high-pressure gradient (HPG) pump system" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_KNAUER_AZURA_1240_bar_UHPLC_System_Binary_high_pressure_gradient_HPG_pump_system_b94a8018c7.jpg 245w," sizes="100vw" width="1280" height="550"></p><h4><strong>Sample Injection</strong></h4><h5><strong>Autosampler 1240 bar</strong></h5><p>The&nbsp;<strong>autosampler AZURA AS 6.1L 1240&nbsp;bar</strong> can inject from up to 768 positions when equipped with microtiter plates or from up to 108 standard 2 ml sample vials. It&nbsp;is equipped with an ILD<sup>TM</sup> valve, consisting of a rotor-stator combination with a central port for depressurizing the sample loop before receiving the sample. This way, the sample is not diluted with solvent. A Cooling/heating option (4–40 °C) is optionally available.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_KNAUER_AZURA_1240_bar_UHPLC_System_autosampler_AZURA_AS_6_1_L_1240_bar_ff1ac03e70.jpg" alt="Knauer: KNAUER AZURA 1240 bar UHPLC System - autosampler AZURA AS 6.1L 1240&nbsp;bar" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_KNAUER_AZURA_1240_bar_UHPLC_System_autosampler_AZURA_AS_6_1_L_1240_bar_ff1ac03e70.jpg 245w," sizes="100vw" width="1280" height="780"></p><h5><strong>Liquid handler&nbsp;</strong></h5><p>The <strong>Liquid handler LH 8.1 </strong>offers automatic sample preparation to speed up your workflow. No sample loss and high precision injection of small and large sample volumes are provided. High-throughput with up to 4 robotic coolers with&nbsp;max. of 24 racks for up to 390 HPLC vials or well plates. Washing station with two different wash solvents minimizes carry-over. Flexibility of modules and functions according to your needs.</p><h4><strong>Column selection and thermostat</strong></h4><h5><strong>Column thermostat</strong></h5><p>The <strong>forced air column thermostat</strong><i> </i><strong>AZURA CT 2.1</strong> is capable of heating or cooling from 5 to 85 °C. The powerful fan and robust peltier element keep the column at a very stable temperature, thus allowing reproducible analysis results.&nbsp;Optional eluent preheating cartridges can be installed.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_KNAUER_AZURA_Educational_HPLC_System_Column_selection_and_thermostat_567c35ab61.jpg" alt="Knauer: KNAUER AZURA Educational HPLC System - Column selection and thermostat" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_KNAUER_AZURA_Educational_HPLC_System_Column_selection_and_thermostat_567c35ab61.jpg 245w," sizes="100vw" width="1700" height="881"></p><h5><strong>Column switching valves</strong></h5><p><strong>KNAUER valves V&nbsp;4.1</strong>&nbsp;and the <strong>KNAUER valve drive VU 4.1&nbsp;</strong>are designed for a wide range of chromatographic applications. Flexibility is provided by choice of different materials and sizes as well as drivers for various software packages.</p><h4><strong>Detection</strong></h4><h5><strong>Diode array detectors (DAD or PDA)</strong></h5><p>The AZURA Diode array detectors (DAD)&nbsp;are high-end diode array detectors with a wavelength range from 190–1000 nm for the <strong>AZURA DAD 6.1L</strong> and 190–700 nm for the <strong>AZURA DAD 2.1L</strong>. The intelligent temperature management of both devices enables fast operational readiness after startup. It also minimizes baseline drift. Due to the frontally accessible deuterium and halogen lamp, the device is easy to service.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_KNAUER_AZURA_1240_bar_UHPLC_System_AZURA_DAD_6_1_L_Diode_array_detectors_DAD_or_PDA_919e55c936.jpg" alt="Knauer: KNAUER AZURA 1240 bar UHPLC System - AZURA DAD 6.1L Diode array detectors (DAD or PDA)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_KNAUER_AZURA_1240_bar_UHPLC_System_AZURA_DAD_6_1_L_Diode_array_detectors_DAD_or_PDA_919e55c936.jpg 245w," sizes="100vw" width="1280" height="387"></p><h5><strong>Mass Detector for MS/MS</strong></h5><p>The <strong>SCIEX Triple Quad™ 4500 MS/MS</strong> system offers superior quantitative performance with high sensitivity combined with excellent robustness and long-term stability. Designed for food, environmental, forensic and clinical research, this MS/MS system enables test development for even the most demanding applications.</p><ul><li>Workhorse triple quad mass spectrometer for dependable everyday use&nbsp;</li><li>High sensitivity for food and environmental contaminant screening and target quantitation</li><li>High scan speed for reliable quantitation of sharp UHPLC peaks</li><li>Simple and fast data processing powered by SCIEX OS software</li></ul><h5><strong>Variable or multiple wavelength detectors (MWD or VWD)</strong></h5><p>The <strong>AZURA MWD 2.1L</strong> is an 8 channel, sensitive multiwavelength detector combining high performance with easy handling at an affordable price. The newly developed optical unit and intelligent temperature management ensure for maximum sensitivity combined with minimal baseline drift.&nbsp;</p><h5><strong>UV/VIS detectors with single variable wavelength</strong></h5><p>The UV/VIS Detector <strong>AZURA UVD 2.1L</strong> is a competitively priced HPLC spectrophotometer for routine HPLC applications including fast LC methods.&nbsp;It comes with an installed deuterium lamp which covers a wavelength range from 190 to 750 nm.&nbsp;Due to a smart design the flow cell is easily accessible and can be changed very quickly.&nbsp;</p><h5><strong>Refractive index detection (RI)</strong></h5><p>The <strong>AZURA RID 2.1L</strong> is a sensitive and competitively priced differential refractometer. It is suitable for detecting compounds with little or no UV activity such as alcohols, sugars, lipids or polymers.&nbsp;The intelligently designed optical unit with advanced temperature control ensures high sensitivity, fast baseline stabilization, and excellent reproducibility. The wide linear dynamic range, its low internal volume of 43 µl from the inlet to the flow cell and 10 ml/min maximum flow rate make the AZURA RID 2.1L the perfect choice for most laboratory tasks.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_KNAUER_AZURA_1240_bar_UHPLC_System_AZURA_RID_2_1_L_Refractive_index_detection_RI_8cd15bf26d.jpg" alt="Knauer: KNAUER AZURA 1240 bar UHPLC System - AZURA RID 2.1L Refractive index detection (RI)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_KNAUER_AZURA_1240_bar_UHPLC_System_AZURA_RID_2_1_L_Refractive_index_detection_RI_8cd15bf26d.jpg 245w," sizes="100vw" width="1280" height="387"></p><h5><strong>Fluorescence detection (FLD)</strong></h5><p>The fluorescence detectors <strong>RF-20A </strong>and <strong>RF-20Axs</strong> provide&nbsp;world-class sensitivity, excellent maintainability and diverse validation / support functions. They&nbsp;support&nbsp;a wide range of applications in the wavelength range of 200 to 750 nm for the RF-20Axs and 200 to 650 nm for the RF-20A from conventional analysis to high-performance analysis.</p><h5><strong>Evaporative light scattering detection (ELSD)&nbsp;</strong></h5><p>As an universal detector, an <strong>ELSD </strong>offers numerous possibilities for detecting substances that have few or no chromophores. Since the eluents are evaporated, the use of non-UV-compatible solvents poses no problems and the ELSD is gradient compatible. KNAUER offers different models for different application tasks.</p><h4><strong>Software solutions</strong></h4><h5><strong>Mobile Control</strong></h5><p>The Mobile Control is an intuitive and cost-effective software solution for controlling and monitoring your AZURA devices and systems. The clear system overview provides instant information on key parameters and system status. Adjust and operate single devices or even whole systems with a few fingertips.&nbsp;The well-structured block programming assists you to clearly and quickly automate device control. A smart user management makes the Mobile Control safe. Device parameters can only be changed by authorized operators.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_KNAUER_AZURA_Educational_HPLC_System_Mobile_Control_software_2052b3ed77.jpg" alt="Knauer: KNAUER AZURA Educational HPLC System - Mobile Control software" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_KNAUER_AZURA_Educational_HPLC_System_Mobile_Control_software_2052b3ed77.jpg 241w," sizes="100vw" width="1280" height="830"></p><h5><strong>ClarityChrom® CDS</strong></h5><p>ClarityChrom is an easy-to-use chromatography data system for workstations. The optional control extensions for GC, PDA and GPC allow to use the software for a wide range of applications.</p><h5><strong>OpenLAB® CDS EZChrom Edition</strong></h5><p>Basic Workstation license, includes System Suitability, Fraction Collector Control and Software Maintenance Agreement</p><h5><strong>Chromeleon™ 7.2</strong></h5><p>Thermo Scientific™ Dionex™ Chromeleon™ is one of the most wide-spread chromatography data systems. Its intuitive handling benefits laboratory workflow and the highly developed algorithms simplify data processing. It offers a broad range of third-party drivers and can be easily used with existing HPLC systems. KNAUER offers drivers for a lot of its devices.&nbsp;</p>

KNAUER AZURA HTQC UHPLC System

KNAUER AZURA HTQC UHPLC (1240 bar) enables high-throughput QC with parallel analysis and column re-equilibration for faster workflows.

<p><strong>The KNAUER AZURA HTQC UHPLC (1240 bar) is a high-performance UHPLC system designed for high-throughput quality control (QC) where speed, robustness, and reproducibility are essential.</strong></p><p>The system features a unique concept of <strong>parallel analysis and offline column re-equilibration</strong>, significantly increasing productivity. With <strong>two gradient pumps</strong>, one column performs the analytical run while the second is <strong>re-equilibrated in backflush mode</strong>.</p><p>This architecture enables:</p><ul><li><strong>≥30% reduction in cycle time</strong></li><li><strong>increased sample throughput</strong></li><li><strong>column protection from matrix contamination</strong></li><li><strong>consistent retention times</strong></li></ul><p>It is ideal for <strong>pharmaceutical QC laboratories</strong> requiring <strong>automation and validated workflows</strong>.</p><h4><span><strong>Key Features</strong></span></h4><ul><li>UHPLC system <strong>up to 1240 bar (124 MPa)</strong></li><li><strong>Dual gradient pumps</strong> (HPG + LPG)</li><li><strong>Parallel analysis</strong> and column re-equilibration</li><li>Backflush mode for column protection</li><li><strong>≥30% faster analysis</strong> cycles</li><li>High flow reproducibility</li><li>Autosampler with cooling</li><li>Column thermostat (Peltier)</li><li><strong>Fully automated modular system</strong></li><li><strong>MS detector compatibility</strong></li></ul><h4><span><strong>System Configuration</strong></span></h4><p>Included components:</p><ul><li>Eluent tray E 2.1L</li><li>Pump P 8.1L (HPG)</li><li>Pump P 6.1L (LPG)</li><li>Liquid handler LH 8.1 + Robotic Cooler</li><li>Column thermostat CT 2.1</li><li>Column switching &amp; backflush valve</li><li>Accessories and tools</li></ul><h3><span><strong>Technical Specifications</strong></span></h3><h4><span><strong>Pumps</strong></span></h4><h5><strong>P 8.1L (HPG – analytical pump):</strong></h5><ul><li><strong>Max pressure:</strong> <strong>1240 bar</strong></li><li><strong>Flow rate:</strong> up to <strong>5 mL/min</strong></li><li>Adaptive pulsation compensation</li><li>Degasser + binary solvent selection</li><li>Low baseline noise</li></ul><h5><strong>P 6.1L (LPG – re-equilibration):</strong></h5><ul><li>Quaternary gradient</li><li>Integrated degasser</li><li>Low dwell volume</li><li>Inline filter</li></ul><h4><span><strong>Autosampler</strong></span></h4><h5><strong>LH 8.1:</strong></h5><ul><li>XYZ autosampler</li><li>Capacity: up to 390 samples</li><li>Temperature: 4–40 °C</li><li>Low carryover</li></ul><h4><span><strong>Column Thermostat</strong></span></h4><h5><strong>CT 2.1:</strong></h5><ul><li>Peltier controlled</li><li>Above and below ambient operation</li><li>High precision</li></ul><h4><span><strong>Valve System</strong></span></h4><ul><li>Column switching + backflush</li><li>Up to 1200 bar</li><li>Parallel column operation</li></ul><h4><span><strong>Detection</strong></span></h4><ul><li>Compatible with all HPLC detectors</li><li>Ideal for LC-MS coupling</li></ul><h4><span><strong>HTQC Concept</strong></span></h4><ul><li>Independent flow paths (analysis vs re-equilibration)</li><li>Backflush re-equilibration</li><li>Parallel operation</li><li>Increased throughput without compromise</li></ul><h4><span><strong>Typical Applications</strong></span></h4><ul><li>Pharmaceutical QC</li><li>Routine validated workflows</li><li>High sample throughput environments</li><li>Reversed-phase HPLC</li></ul>

Products from category UHPLC from KNAUER - page 1

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