<div><p><strong>Understanding how to read the resulting data of high-performance liquid chromatography (HPLC) helps turn raw detector output into confident decisions about purity, identity, and assay results. When you know how to read HPLC peaks and baselines, you can quickly spot problems, verify method performance, and support regulatory‑ready reports.</strong></p></div><div><div><div><div><h3><strong>What is an HPLC Chromatogram?</strong></h3></div></div></div></div><div><p>An HPLC chromatogram is a plot of detector signal versus time that shows how each compound in your sample moves through the column and reaches the detector. Every peak corresponds to a different component, with the position on the time axis giving its retention time and the peak size relating to concentration.</p><p>In routine work, you read retention time to confirm identity and rely on peak area or height for concentration, so learning how to read an HPLC chromatogram is essential for everyday assay workflows.</p></div><div><div><div><div><h3><strong>Why Is It Important to Read HPLC Chromatograms Correctly?</strong></h3><p>Reading HPLC results correctly matters because retention time and peak response are key parameters for separating, identifying, and quantifying compounds in complex mixtures. Misreading the baseline or peak shape can distort area integration, which affects assay accuracy and impurity quantitation.</p><p>Good <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/knowledge-center/hplc-knowledge-center/what-are-chromatograms"><strong>chromatogram</strong></a> interpretation also helps you detect method drift early, keep system suitability within limits, and avoid costly repeat injections or failed batches.</p></div></div></div></div><div><div><div><div><h3><strong>Key Components of an HPLC Chromatogram</strong></h3></div></div></div></div><div><p>Before you interpret a run, you must recognize the fundamental parts of a chromatogram. Each visual element gives you specific data about the separation and the analytes present in your sample.</p><h4><strong>Baseline</strong></h4><p>The baseline is the detector signal when no analyte peak is eluting, and a stable, flat baseline makes integration and quantification reliable. More detailed descriptions of baseline noise and drift are covered in the troubleshooting section below. In a nutshell, it is enough to note that any instability can affect how confidently you integrate and compare peaks.</p><h4><strong>Peaks</strong></h4><p>Peaks represent eluting analytes and appear as Gaussian‑like shapes rising above the baseline. Symmetric peaks with clear separation are easier to integrate and give more reliable responses for quantitative work.</p><h4><strong>Retention Time</strong></h4><p>Retention time, <i>t</i><sub>R</sub>, is the time from injection until the apex of a peak, reflecting how long an analyte spends in the column compared with the mobile phase. The solvent front or void peak corresponds to the dead time, <i>t</i><sub>0</sub>, which is the passage of unretained material through the system.</p><h4><strong>Peak Area and Height</strong></h4><p>Peak area is generally more reliable than peak height for quantitative analysis, because it better reflects the total amount of analyte passing the detector. In validated methods, you relate peak area or, in some cases, peak height to concentration through calibration curves and internal standards.</p><h4><strong>Resolution</strong></h4><p>Resolution describes how well two peaks are separated and depends on efficiency, selectivity, and retention. Adequate resolution means peaks do not overlap significantly, which improves identification confidence and quantification for closely eluting impurities.</p><h4><strong>Dead Time (t₀)/Void Time</strong></h4><p>Dead time, <i>t</i><sub>0</sub>, or hold‑up time, <i>t</i><sub>M</sub> (or void time), is the time taken by unretained species or the mobile phase front to pass through the column and reach the detector. You use <i>t</i><sub>0</sub> as the reference for calculating capacity factor and for assessing how strongly analytes interact with the stationary phase.</p></div><div><div><div><div><h3><strong>Step‑by‑Step Guide to Reading an HPLC Chromatogram</strong></h3></div></div></div></div><div><p>Interpreting your data requires a systematic approach to guarantee you capture all critical details. Follow this sequential process to evaluate your chromatographic results confidently and accurately.</p><h4><strong>Identify the Baseline Stability</strong></h4><p>Start by checking if the baseline is flat or if you see random noise and slow drift across the run. Significant drift or high noise suggests issues with temperature control, solvent quality, or detector stability, which must be addressed before trusting quantitation.</p><h4><strong>Locate and Count Peaks</strong></h4><p>Next, scan the chromatogram to locate all distinct peaks above the baseline and note their relative sizes. Counting peaks versus expectations from standards or sample composition helps you detect co‑elution, extra peaks, or missing analytes.</p><h4><strong>Determine Retention Time</strong></h4><p>Measure retention time, <i>t</i><sub>R</sub>, for each peak and record these values alongside known standards or system-suitability data. Consistent <i>t</i><sub>R</sub> values within defined windows support correct identification, while larger shifts can indicate changes in temperature, mobile phase composition, or flow rate.</p><h4><strong>Calculate Capacity Factor (k') Using t<sub>M</sub> and t<sub>R</sub></strong></h4><p>Capacity factor, <i>K'</i> (or retention factor), describes how much longer an analyte is retained compared with unretained material and is calculated from the chromatogram. Using the dead time, <i>t</i><sub>M</sub>, (void peak) and analyte retention time, <i>t</i><sub>R</sub>, you apply<strong> </strong><i><strong>k</strong></i><strong> = (</strong><i><strong>t</strong></i><strong><sub>R</sub> – </strong><i><strong>t</strong></i><strong><sub>M</sub>)/</strong><i><strong>t</strong></i><strong><sub>M</sub></strong> to assess whether the analyte is sufficiently retained under the chosen conditions.</p><p>Knowing how to analyze HPLC results with capacity factor helps you judge if peaks elute too close to <i>t</i><sub>0</sub>, or spend an appropriate time interacting with the column, which guides method refinement.</p><h4><strong>Analyze Peak Area for Quantification (H3)</strong></h4><p>For quantification, integrate each peak to obtain the area and compare it with calibration standards or internal standards to calculate concentration. Reliable peak area measurements depend on good baseline definition and stable detector response, so always verify baseline quality before finalizing your measurements.</p><h4><strong>Evaluate Peak Shape and Resolution</strong></h4><p>Assess whether peaks are symmetric or show tailing, fronting, or broadening, and examine spacing between adjacent peaks for resolution. Changes in peak shape across the chromatogram can reveal extra‑column effects, packed bed issues, or interaction with active sites on the stationary phase.</p><p>If you see persistent problems, review your <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/products?category=48&amp;manufacturerItems=50"><strong><u>HPLC column</u></strong></a> choice, sample preparation, and gradient or isocratic settings to refine separation.</p></div><div><div><div><div><h3><strong>Common Problems in HPLC Chromatograms and How to Identify Them</strong></h3><p>Even well-developed methods can degrade over time and produce visual cues that signal system or chemistry faults. Recognizing these characteristic peak and baseline distortions is your first step in troubleshooting.</p><h4><strong>Peak Tailing</strong></h4><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/knowledge-center/hplc-knowledge-center/how-to-reduce-peak-tailing-in-hplc"><strong><u>Peak tailing</u></strong></a> appears when the right side of a peak stretches out into a long tail instead of dropping back to the baseline symmetrically. On the chromatogram, the trailing edge slopes slowly and the peak looks skewed to the left, which makes integration boundaries less obvious and can hint at dispersion or secondary interactions. You can reduce tailing by minimizing extra‑column volume and checking column packing and active sites.</p><h4><strong>Peak Fronting</strong></h4><p><a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/knowledge-center/hplc-knowledge-center/peak-fronting"><strong><u>Peak fronting</u></strong></a> shows a steep leading edge and a compressed front portion of the peak, so the apex seems pulled forward relative to the rest of the peak profile. Visually, the peak looks like it is leaning in the direction of flow and the front overlaps more strongly with neighboring peaks, which can distort apparent purity for fast‑eluting components.</p><p>Consider lowering sample load or injection volume and matching diluent strength to the mobile phase, and when fronting persists, consult Phenomenex resources on <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/knowledge-center/gc-knowledge-center/peak-shape-problems-tailing-peaks"><strong><u>peak-shape problems</u></strong></a>.</p><h4><strong>Noise and Drift</strong></h4><p>Baseline noise appears as small, rapid fluctuations around the baseline that make it harder to see low‑level peaks. Baseline drift shows up as a slow rise or fall of the baseline across the run, so the line no longer stays flat; this can cause apparent changes in peak height and shifts in where you draw integration baselines. Simple checks like improving solvent degassing, stabilizing temperature, and cleaning detector flow cells help reduce noise and drift.</p><h4><strong>Co‑Elution</strong></h4><p>Co‑elution is easy to spot when two peaks do not fully separate and instead form a shoulder or merged profile on the chromatogram.You may see one peak with a plateau or a visible kink where two components overlap, and the valley between them does not return to the baseline, which complicates assignments for closely eluting impurities. To resolve co‑elution, adjust selectivity by changing mobile phase composition, gradient profile, or column chemistry.</p><h4><strong>Broad Peaks</strong></h4><p>Broad peaks have a noticeably wider base and lower height compared with well‑focused peaks of similar are. They often span more time units on the x‑axis and show gentle slopes on both sides, indicating band spreading that reduces resolution and makes small shoulders harder to detect. Narrowing broad peaks usually involves reducing extra‑column volumes and confirming column performance and particle size suitability.</p><h4><strong>Retention-Time Shifting</strong></h4><p>Retention-time shifting is seen when all peaks move earlier or later in the chromatogram compared with previous runs, while their shapes remain similar. When you overlay chromatograms, consistent shifts in peak positions along the time axis, often on the order of a few percent, indicate changes in conditions such as temperature or mobile phase that must be tracked carefully. Fixing retention time shifts starts with stabilizing temperature and verifying mobile phase composition and flow rate settings across runs.</p></div></div></div></div><div><div><div><div><h3><strong>Tips for Accurate HPLC Chromatogram Interpretation</strong></h3></div></div></div></div><div><p>To read HPLC chromatograms accurately, maintain a stable temperature, use well‑degassed mobile phases, and keep detector flow cells and optics clean. Regularly check capacity factor and selectivity values against method expectations, and verify that resolution is adequate for critical pairs before accepting results.</p><p>When retention times drift or peak shapes degrade, review your <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/resources/knowledge-center/gc-knowledge-center/understanding-retention-time"><strong><u>retention-time</u></strong></a> trends alongside system maintenance logs so you can correct problems early.</p></div><div><div><div><div><h3><strong>FAQs on Reading HPLC Chromatograms</strong></h3></div></div></div></div><div><h4><strong>How Do You Calculate Capacity Factor (k') from a Chromatogram?</strong></h4><p>Capacity factor, <i>k</i>, is calculated using the analyte retention time, [Equation], and the dead time, <i>t</i><sub>R</sub>, measured from the unretained peak. Apply <i>k</i> = (<i>t</i><sub>R</sub> – <i>t</i><sub>M</sub>)/<i>t</i><sub>M</sub>, so higher k values indicate stronger interactions with the stationary phase and longer retention.</p><h4><strong>How Is Selectivity (α) Calculated Using Chromatogram Data?</strong></h4><p>Selectivity (α) describes how well two components are separated and is defined as the ratio of their retention factors, α = <i>k</i><sub>2</sub>/<i>k</i><sub>1</sub>, for peaks, where <i>k</i><sub>2</sub> &gt; <i>k</i><sub>1</sub>. Values higher than 1.2 show separation, and larger α values correspond to better selectivity between the peaks.</p><h4><strong>What Does Peak Area Indicate in HPLC?</strong></h4><p>Peak area reflects the total amount of analyte that reaches the detector and is generally more reliable than peak height for quantitative work. Build calibration curves that relate peak area to concentration, then apply them only when the baseline and detector response remain stable across all injections.</p><h4><strong>How Is Retention Time Used in HPLC Analysis?</strong></h4><p>Retention time is used as a qualitative index because each compound elutes at a characteristic time when conditions are held constant. Compare sample retention times with those of standards and then pair that information with peak area or height to confirm identity and calculate concentration in routine analyses.</p></div>

How to Read an HPLC Chromatogram: Step‑by‑Step Guide to Interpreting Results

<p><i><span><strong>Are you currently using single-column batch chromatography to isolate and purify target compounds, but struggling with high solvent demand, low product concentration, or limited throughput? Simulated Moving Bed chromatography offers an alternative approach that can address many of these limitations through continuous multi-column separation.</strong></span></i></p><h4><span><strong>What is Simulated Moving Bed Chromatography (SMB)?</strong></span></h4><p><span>Simulated Moving Bed (SMB) chromatography is a continuous multi-column separation technique designed to make more efficient use of the stationary phase while minimizing solvent consumption. It is particularly well suited to the purification of binary and pseudo-binary mixtures, including enantiomers, sugars, petrochemical products, and fine chemicals.</span></p><h4><span><strong>Conventional Batch Chromatography</strong></span></h4><p><span>In traditional batch chromatography, the sample is introduced onto a single chromatographic column and transported through the stationary phase by the mobile phase. Individual compounds interact with the stationary phase to different extents and therefore travel through the column at different rates:</span></p><ul><li><span>Weakly retained compounds migrate more rapidly.</span></li><li><span>Strongly retained compounds move through the column more slowly.</span></li></ul><p><span>The separated substances consequently leave the column at different retention times and can be collected as individual fractions (Fig. 1). Before a new sample is normally introduced, the previous sample must be completely eluted and the column prepared for the next separation.</span></p><p><span>Although single-column batch chromatography is versatile and comparatively easy to operate, several disadvantages become increasingly important at production scale. The stationary phase is not continuously involved in useful separation, which can limit productivity. Other drawbacks include substantial solvent consumption, diluted product fractions, repeated column regeneration, and potential loss of valuable product.</span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_Single_column_separation_A_fast_eluting_component_B_retaining_component_cda8ef66ef.jpg" alt="KNAUER: Figure 1: Single column separation; A – fast eluting component, B – retaining component" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_Single_column_separation_A_fast_eluting_component_B_retaining_component_cda8ef66ef.jpg 76w," sizes="100vw" width="394" height="800"></p><h4><span><strong>From True Moving Bed to SMB</strong></span></h4><p><span>The principle behind SMB originates from the theoretical <strong>True Moving Bed (TMB)</strong> process. In an ideal TMB system, the liquid mobile phase and the solid stationary phase continuously travel in opposite directions. This counter-current arrangement takes advantage of differences in the affinity of individual compounds toward the two phases.</span></p><p><span>Consider a binary mixture containing components A and B. Component A interacts more strongly with the stationary phase, whereas component B is less strongly retained and preferentially follows the liquid mobile phase. Under appropriate operating conditions, the two substances effectively migrate in opposite directions and can be collected continuously through separate outlets (Fig. 2).</span></p><p><span>While this concept is highly efficient from a theoretical perspective, continuously moving a packed solid chromatographic adsorbent is technically difficult to realize.</span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_True_Moving_Bed_concept_e1eac66028.jpg" alt="KNAUER: Figure 2: True Moving Bed concept" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_True_Moving_Bed_concept_e1eac66028.jpg 186w," sizes="100vw" width="954" height="800"></p><p><span>Four process ports divide the system into four functional zones (Zone I–IV):</span></p><ol><li><span>Eluent (desorbent) inlet</span></li><li><span>Extract outlet</span></li><li><span>Feed inlet</span></li><li><span>Raffinate outlet</span></li></ol><p><span>Feed and eluent are introduced continuously, while two product streams are simultaneously withdrawn:</span></p><ul><li><span><strong>Extract</strong> – the strongly retained, later-eluting component</span></li><li><span><strong>Raffinate</strong> – the less strongly retained, earlier-eluting component</span></li></ul><h4><span><strong>The Four Functional Zones</strong></span></h4><p><span>Each of the four zones has a defined purpose and operates at its own internal flow rate (VI–VIV):</span></p><ul><li><span><strong>Zone I</strong> regenerates the stationary phase.</span></li><li><span><strong>Zone II</strong> prevents the weakly retained component from entering the extract stream.</span></li><li><span><strong>Zone III</strong> prevents the strongly retained component from contaminating the raffinate.</span></li><li><span><strong>Zone IV</strong> enables recovery and internal reuse of the remaining solvent.</span></li></ul><h4><span><strong>How is the TMB Principle Implemented in SMB?</strong></span></h4><p><a target="_blank" rel="noopener noreferrer" href="https://www.knauer.net/smb"><span>SMB</span></a><span> eliminates the need to physically move the stationary phase. Instead, the adsorbent remains fixed inside the chromatographic columns, while the positions of the liquid inlet and outlet ports are periodically shifted.</span></p><p><span>From the perspective of these moving connection points, the stationary phase appears to travel in the opposite direction to the liquid phase. SMB therefore reproduces the counter-current behavior of a TMB system without physically transporting the packed adsorbent (Fig. 3).</span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Transfer_TMB_concept_to_SMB_concept_e4b8905269.jpg" alt="KNAUER: Figure 3: Transfer TMB concept to SMB concept" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Transfer_TMB_concept_to_SMB_concept_e4b8905269.jpg 245w," sizes="100vw" width="1372" height="800"></p><p><span>A typical SMB setup consists of several chromatographic columns arranged in a closed loop. A standard configuration often uses eight columns, although the number can be adapted according to the application.</span></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_SMB_concept_fb005cdfa1.jpg" alt="KNAUER: Figure 4: SMB concept" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_SMB_concept_fb005cdfa1.jpg 195w," sizes="100vw" width="1001" height="800"></p><p><span>After a predefined switching time, all inlet and outlet positions move forward by one column in the direction of the mobile-phase flow. The columns themselves stay in place, but their functional roles within the process change.</span></p><p><span>By repeatedly shifting the ports, the system creates the effect of a stationary phase moving counter-currently to the mobile phase. With eight columns, eight switching steps are required to complete one full SMB cycle.</span></p><p><span>Following an initial transition period, the process reaches a cyclic steady state. Concentrations continue to fluctuate during individual switching intervals, but the overall concentration profile repeats after every complete cycle.</span></p><p><span>Increasing the number of columns and reducing the size of individual switching steps allows the SMB system to reproduce ideal TMB behavior more closely. Systems containing fewer columns exhibit more pronounced discrete switching effects and can require more sophisticated dynamic modeling during process development.</span></p><h3><span><strong>What are the Main Advantages of SMB?</strong></span></h3><h4><span><strong>1. Continuous Operation and Higher Productivity</strong></span></h4><p><span>The key benefit of SMB chromatography is continuous operation. Feed and eluent enter the system continuously, while purified extract and raffinate streams are removed at the same time.</span></p><p><span>As a result, SMB systems can operate for prolonged periods—from several days to even several weeks—with very limited interruption.</span></p><p><span>Because several chromatographic columns perform different separation functions simultaneously, the stationary phase is utilized much more effectively than in conventional batch chromatography. This can translate into higher productivity, improved process efficiency, and a continuous supply of purified product.</span></p><h4><span><strong>2. Reduced Solvent Consumption</strong></span></h4><p><span>Conventional batch chromatography can require substantial volumes of mobile phase to achieve separation and product recovery. The collected fractions are often highly diluted, creating an additional need for downstream concentration.</span></p><p><span>SMB maintains concentration profiles within the circulating system and uses the mobile phase more efficiently. Partial internal recycling of solvent is an inherent part of the process.</span></p><p><span>Lower solvent consumption can lead to:</span></p><ul><li><span>Reduced raw-material costs</span></li><li><span>Lower volumes of liquid waste</span></li><li><span>Reduced energy requirements for solvent evaporation</span></li><li><span>More concentrated product streams and less downstream concentration</span></li></ul><h4><span><strong>3. High Recovery and Reproducible Product Quality</strong></span></h4><p><span>Once the process reaches cyclic steady state, SMB can continuously generate product streams with stable and reproducible composition.</span></p><p><span>Additional advantages can include high product recovery, lower product dilution, more efficient use of the stationary phase, and straightforward integration into continuous manufacturing strategies.</span></p><h4><span><strong>What are the Differences Between Batch LC and SMB?</strong></span></h4><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_differences_between_batch_LC_and_SMB_eba3897f65.jpg" alt="KNAUER: Differences between batch LC and SMB" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_differences_between_batch_LC_and_SMB_eba3897f65.jpg 245w," sizes="100vw" width="1061" height="638"></p><h4><span><strong>Where is SMB Used?</strong></span></h4><p><span>SMB technology is established across several industries and can be applied to purification processes ranging from gram-scale production to several tons per day (Fig. 5).</span></p><p><span>Typical and emerging applications include:</span></p><ul><li><span>Sugar purification</span></li><li><span>Petrochemical separations</span></li><li><span>Chiral separations</span></li><li><span>Fine chemical production</span></li><li><span>Pharmaceutical intermediates</span></li><li><span>Selected biomolecule separations</span></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_SMB_application_areas_7109ff703f.jpg" alt="KNAUER: Figure 5: SMB application areas; author's illustration" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_SMB_application_areas_7109ff703f.jpg 245w," sizes="100vw" width="1500" height="512"></p><h4><span><strong>Limitations of SMB</strong></span></h4><p><span>Although SMB can provide substantial advantages, it is not necessarily the ideal solution for every chromatographic separation.</span></p><p><span>The instrumentation and process configuration are more complex than conventional single-column chromatography. Several pumps, valves, columns, and liquid pathways must be controlled precisely. Successful method development therefore requires a solid understanding of adsorption equilibria, mass-transfer effects, and fluid dynamics.</span></p><p><span>Classical four-zone SMB is primarily intended for binary and pseudo-binary separations. More complicated mixtures may require modified SMB configurations, additional purification steps, or multiple coupled SMB systems.</span></p><p><span>SMB is generally most economically attractive when relatively large quantities of a consistent feed material are processed continuously over extended periods.</span></p><p><span>Batch chromatography can remain the better option for small-scale production, frequent changes between products, complex multicomponent mixtures, or applications that depend on flexible gradient separations.</span></p><h4><span><strong>Conclusion</strong></span></h4><p><span>Simulated Moving Bed chromatography converts the theoretical counter-current principle of True Moving Bed separation into a practical continuous chromatographic process. Instead of physically transporting the stationary phase, SMB periodically changes the positions of the feed, eluent, extract, and raffinate connections. This reproduces the effect of counter-current movement between stationary and mobile phases.</span></p><p><span>Compared with conventional batch chromatography, SMB can provide higher productivity, more efficient stationary-phase utilization, substantially lower solvent consumption, and more concentrated product streams. Continuous operation makes the technology particularly attractive for demanding large-scale binary separations, including the purification of pharmaceutical enantiomers.</span></p><p><span>Although successful SMB implementation requires accurate process data, careful method development, and precise control of operating conditions, the technology demonstrates how an idealized chromatographic principle can be transformed into a robust and efficient industrial separation strategy.</span></p>

Simulated Moving Bed Chromatography: Efficient Continuous Separation

News from LabRulezLCMS Library - Week 44, 2026

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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Waters ACQUITY Arc LC System

The ACQUITY™ Arc™ System is an all-in-one platform for HPLC and UHPLC, designed for robust method development and seamless transfer between modern LC systems, addressing a wide range of analytical challenges.

<p class="cke-user_style-important"><strong>Built upon a foundation of reliability</strong></p><p data-pm-slice="1 1 []"><strong>The method development process is changing. New regulations, software and analytical technologies are rapidly changing how methods are designed, transferred, and monitored over their lifecycle. An all-in-one platform for HPLC and UHPLC separations, the ACQUITY™ Arc™ System is designed to meet the spectrum of challenges you face from robust method development to seamless transfer between any modern LC systems.</strong></p><p data-pm-slice="1 1 []">With the <strong>ACQUITY Arc System</strong>, you can experience true plug-and-play method compatibility for HPLC and UHPLC separations. Significant productivity gains can be realized by deploying a single LC platform that allows for the efficient transfer, adjustment, and improvement of methods from any LC platform without compromise in method integrity.</p><figure class="image"><img style="aspect-ratio:768/886;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Arc_System_63246061ea.jpg" alt="Waters ACQUITY Arc System" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Arc_System_63246061ea.jpg 136w," sizes="100vw" width="768" height="886"></figure><p data-pm-slice="1 1 []">Laboratory-driven organizations have relied on Waters for over 60 years to deliver reliable, robust, and reproducible solutions to ensure confidence in their analytical results. With over 100,000 LC systems installed, we understand the significant impact the right technology has on your business, and the implications it has on characterizing the quality and safety of your products.</p><p data-pm-slice="1 1 []">Methods with greater agility, robustness, and capacity for change can help you reduce regulatory burden, risk of failure and costs. Visit <a disabled="true">www.methods.waters.com</a> to learn more about how method life cycle management (MLCM) connects your method today to your method tomorrow.</p><h3><strong>Designed for discovery to routine QC applications</strong></h3><p>The <strong>ACQUITY Arc System</strong> is the only available LC system with regulatory compliant-ready hardware and software for both HPLC and UHPLC separations in one instrument. With unrivaled column capacity and Arc Multi-flow path™ technology, the<strong> ACQUITY Arc System</strong> allows you to generate new methods and modernize legacy HPLC methods faster than ever before.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Arc_System_2_c06ba42baa.jpg" alt="Waters ACQUITY Arc System: The ACQUITY Arc System can replicate your established HPLC methods by compensating for these differences with unique features such as the Arc Multi-Flow Path technology and Gradient SmartStart. These innovative attributes enable seamless replication of your existing chromatographic results to meet your acceptance criteria without changes to validated gradient tables." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Arc_System_2_c06ba42baa.jpg 234w," sizes="100vw" width="1024" height="682"></p><h4><strong>Comprehensive detector portfolio</strong></h4><p>High-performance analytical detectors designed to maximize HPLC and UHPLC performance, delivering exceptional sensitivity and linearity for your assays.</p><p>Photodiode array, UV/Vis, fluorescence, refractive index, evaporative light scattering, and mass detection, including the ACQUITY QDa™ Detector.</p><h4><strong>Gradient SmartStart</strong></h4><p>Automatically manage gradient start time and pre-injection steps to maximize sample throughput. Automatically counteract differences in system dwell volume without the need to alter gradient table inputs.</p><h4><strong>Quaternary solvent management</strong></h4><p>Precise and accurate blending of up to four solvents with automated solvent compressibility compensation. Increase solvent flexibility with an optional, integrated solvent select valve, providing access to six additional solvents.</p><h4><strong>Negligible carryover</strong></h4><p>Advanced flow-through needle design minimizes carryover by continuously cleansing the needle during run. User-configurable wash settings provide flexibility to address even the most complex sample matrices.</p><h4><strong>Thermal management options</strong></h4><p>Column manager options available for up to 15 columns ranging in length from 50 mm to 300 mm, with a temperature range of 4 °C to 90 °C. Integrated column selection valve options provide unattended, fast column changeover.</p><h4><strong>Auto•Blend™ Plus Technology</strong></h4><p>Program gradients directly in terms of pH and ionic strength to minimize manual mobile phase preparation, reduce potential for human error, and accelerate method robustness testing for chromatographic methods.</p><h4><strong>Arc Multi-flow path technology</strong></h4><p>Delivers plug-and-play method compatibility with HPLC or UHPLC methods with the flip of a switch.</p><p>Easily replicate or improve established methods by simply selecting between Path 1 (HPLC) or Path 2 (UHPLC) without any manual user intervention.</p><h4><strong>Column Technology</strong></h4><p>Industry-leading selectivity choices to meet the needs of virtually every LC application.</p><h4><strong>Laboratory Informatics</strong></h4><p>Software control within your existing Empower™ or MassLynx™ infrastructure.</p><h3><strong>Replicate your established methods</strong></h3><p data-pm-slice="1 1 []">The <strong>ACQUITY Arc System</strong>, enabled by unique Arc Multi-flow path technology, easily accepts and replicates methods from any HPLC platform, without compromise.&nbsp;</p><p data-pm-slice="1 1 []">Whether you are trying to replicate an out-of-date HPLC method, transfer methods across the organization, or to contract partners, the <strong>ACQUITY Arc System</strong> gives you ultimate flexibility to reproduce, modernize, and transfer existing methods with ease and greater cost efficiency, while preserving the integrity of your methods.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Arc_System_3_c8c7901de3.jpg" alt="Waters ACQUITY Arc System: Analysis run on a Vendor X LC system and replicated on the ACQUITY Arc System. Conditions: 15 to 35% B in 3 min, 35 to 95% B in 2 min; Mobile phase A: 0.1% HCOOH in H 2 O, mobile phase B: 0.1% HCOOH in ACN; Flow rate = 2.0 mL/min; Column: XSelect™ CSH C 18 , 4.6 x 150 mm, 5 μm; Temp. = 30 °C; UV@ 260 nm; 10.0 μL injection volume." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Arc_System_3_c8c7901de3.jpg 245w," sizes="100vw" width="1099" height="596"></p><p>Upgrade your laboratory with a versatile, modern LC system that delivers HPLC and UHPLC method compatibility at the flip of a switch.</p><h3><strong>Modernize your methods</strong></h3><p>The United States Pharmacopeia (USP) is championing pharmacopeial harmonization and modernization efforts, and international organizations are following suit. As a result, the USP is encouraging the modernization of older compendial methods to ensure they are fit for purpose.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Arc_System_4_a1aaab8d02.jpg" alt="Waters ACQUITY Arc System: Quetiapine fumarate system suitability solution analyzed on the ACQUITY Arc System using HPLC, UHPLC, and UPLC™ column/method conditions for the quetiapine impurity method. Peak identification: (1) quetiapine related compound G, (2) quetiapine related compound B, (3) quetiapine desethoxy, and (4) quetiapine." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Arc_System_4_a1aaab8d02.jpg 245w," sizes="100vw" width="1092" height="678"></p><p>Revisions to these guidelines give you more flexibility to modify your existing pharmacopeial methods with modern techniques and technologies, and in some cases avoid revalidation. The <strong>ACQUITY Arc System</strong>, featuring the Waters Columns Calculator, allows you to confidently scale your gradient methods from a 3.5 μm to a 2.5 μm particle, decreasing run times, lowering solvent consumption, and increasing sample throughput.</p><h3><span><strong>Save time. Reduce cost.</strong></span></h3><p data-pm-slice="1 1 []">With the right column, the <strong>ACQUITY Arc System</strong> is the ideal solution to accommodate methods from any LC platform.&nbsp;</p><p data-pm-slice="1 1 []">From concept to production, the transition your product takes through the product development cycle often requires rigorous LC assays to properly characterize and assess its readiness to advance to the next stage. These LC assays are often adapted to accommodate the technology available from one stage to the next, in some cases requiring an adjustment from a smaller particle size (sub-2-μm) to a larger particle size (2.5 μm–5 μm).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Arc_System_5_d9dd95135c.jpg" alt="Waters ACQUITY Arc System: The ACQUITY Arc System enables the support of HPLC and UHPLC methods on a single platform." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Arc_System_5_d9dd95135c.jpg 173w," sizes="100vw" width="959" height="860"></p><p>The <strong>ACQUITY Arc System</strong> provides the flexibility to maximize productivity through efficient and rapid 2.5 μm–2.7 μm UHPLC separations, or to accommodate larger 3.0 μm–5 μm HPLC particles.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Arc_System_6_1ebc2c67e7.jpg" alt="Waters ACQUITY Arc System: Save time. Reduce cost." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Arc_System_6_1ebc2c67e7.jpg 245w," sizes="100vw" width="1683" height="469"></p><h3><span><strong>Mitigate your risk</strong></span></h3><p data-pm-slice="1 1 []">With an expanded range of column manager options enabling up to 15-column switching from 4 °C to 90 °C, the <strong>ACQUITY Arc System</strong> will help you significantly reduce the time spent generating robust methods. Equipped with the plug-and-play ACQUITY QDa Mass Detector, the <strong>ACQUITY Arc System</strong> tracks peaks in your method and reveals hidden coelutions of similar analytes, ensuring you have complete confidence in the reliability and reproducibility of your method now and far into the future.</p><h4><strong>Thermal management options</strong></h4><p data-pm-slice="1 1 []">Active and passive pre-heating options available, with up to 15-column switching from 4 °C to 90 °C. Automated monitoring and tracking of column usage with eCord™ technology available with active pre-heating options.</p><h4><strong>Quaternary solvent management</strong></h4><p data-pm-slice="1 1 []">Accelerate your method development with precise and accurate blending of up to four solvents. Increase solvent flexibility with an optional solvent select valve, providing access to six additional solvents.</p><h4><strong>Auto•Blend Plus Technology</strong></h4><p data-pm-slice="1 1 []">Reduce human error and accelerate method robustness testing for chromatographic methods by programming gradients to control mobile phase pH and ionic strength.</p><h4><strong>Comprehensive detector portfolio</strong></h4><p>Diverse portfolio of detectors for method development, method transfer, and routine use.</p><h4><strong>ACQUITY QDa Detector</strong></h4><p>Mass detection built around the needs of analytical scientists for chromatographic analysis. Robust, reliable and requiring no adjustments, it easily integrates with your<strong> ACQUITY Arc System.</strong></p><h5><strong>Support</strong></h5><p data-start="0" data-end="175">Empower eLearning modules to help train your lab on Empower 3 and Method Validation. Applications support that’s customized based on your method development needs.</p><h5><strong>Consumables</strong></h5><p data-start="177" data-end="410" data-is-last-node="">Industry-leading selectivity choices to meet the needs of virtually every LC application. Quality Control (QC) Reference Material benchmarks your <strong>ACQUITY Arc System </strong>and enables perfect resolution. Certified clean vials.</p><h5><strong>Laboratory Informatics</strong></h5><p>Software control within your existing Empower or MassLynx infrastructure. Empower 3 Method Validation Manager allows you to perform chromatographic method validation in one application. Optional S-Matrix Fusion Software automates your method development according to AQbD guidelines.</p><h3><span><strong>Develop methods that stand the test of time</strong></span></h3><p>Need an orthogonal detection approach to UV in your method development? Streamline your method development with an LC-MS solution that’s as easy to use as photodiode array detection.</p><p>The <strong>ACQUITY Arc System</strong> is fully compatible with the ACQUITY Arc, supporting efficient and robust method development by expanding the information you can get from your system using the same Empower Software. The system automatically pairs qualitative mass spectral data to the peaks in your chromatogram, leaving nothing to chance.</p><ul><li>Confirm the identity of peaks in a separation without injecting standards.</li><li>Identify new peaks in a separation with molecular weight information.&nbsp;</li><li>Detect unresolved peaks and determine if a peak is homogeneously pure.</li><li>Easily track peaks as method conditions are changed.</li></ul><p data-start="272" data-end="600">Using Empower 3 Software and its Custom Calculations and Custom Reporting functions enables you to quickly and automatically evaluate chromatographic data. Configurable reports allow you to choose optimal conditions based on metrics rather than judgment, ensuring a comprehensive assessment of data and best method conditions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Arc_System_7_e7ef959203.jpg" alt="Waters ACQUITY Arc System: Method transfer of USP Assay for Fluconazole using the ACQUITY Arc System. Here the peak has same m/z of a related compound. Conditions: Mobile phase: 20:80 acetonitrile/ water; Flow rate = 0.5 mL/min; Column: XSelect HSS T3, 4.6 x 150 mm, 3.5 μm; UV@ 260 nm; 20.0 μL injection volume." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Arc_System_7_e7ef959203.jpg 224w," sizes="100vw" width="939" height="654"></p><h3><strong>Streamline your workflow</strong></h3><div><div data-message-author-role="assistant" data-message-id="7b732be9-0a45-45fb-b585-70a90c1a74d6" dir="auto" data-message-model-slug="gpt-4o"><div><div><p data-start="602" data-end="827">How would you benefit from increased assay consistency between analysts and from lab to lab? What would you gain by accelerating your robustness testing prior to transferring methods to QA/QC?</p><p data-start="829" data-end="1392" data-is-last-node="">Auto•Blend Plus Technology is a novel software tool that extends traditional quaternary solvent blending to permit gradient programming directly in units of pH and ionic strength, providing the ability to vary composition in even the smallest increments of pH (even those differing by 0.1 pH units or less).&nbsp;</p><p data-start="829" data-end="1392" data-is-last-node="">With Auto•Blend Plus, the cumbersome and error-prone approach of manually preparing several pre-mixed mobile phases can now be eliminated with on-demand mobile phase creation, providing significant time savings and improved productivity to the laboratory.</p></div></div></div></div>

Waters Alliance HPLC System

 With its proven performance history, the Alliance HPLC is the industry’s analytical HPLC system of choice, ideally suited for methods development and routine analysis.

<div><div><h4><strong>Reliably achieve quality, compliant data. Every time, in every lab.</strong></h4></div></div><div><div><p><strong>Liquid chromatography (LC) systems must deliver unequivocal results that give you confidence in your scientific and business decisions. Waters LC commitment continues to succeed with the latest Alliance HPLC System – trusted by scientists worldwide for the data to drive informed decisions.</strong></p><div><div><div><div><p>Building on <strong>industry-standard performance and reliability</strong>, the <strong>Alliance HPLC System</strong> provides the <strong>capabilities to meet today’s business challenges</strong>. With its <strong>proven performance history</strong>, the Alliance HPLC is the <strong>industry’s analytical HPLC system of choice</strong>, ideally suited for <strong>methods development and routine analysis</strong>.</p><ul><li>Time-tested, dependable, and robust workhorse for your HPLC separations</li><li>Integrated solvent and sample management with consistent system-to-system performance and high reproducibility</li><li>Simplify your daily LC system start-up with advanced software functionality</li></ul><h5><strong>Recommended Use</strong></h5><p>For standard HPLC workflows where <strong>consistency and compliance</strong> are the highest priorities.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_HPLC_System_1_6b63ab6d62/Waters-Alliance-HPLC-System-1.jpg" alt="Waters Alliance HPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_HPLC_System_1_6b63ab6d62/thumbnail_Waters-Alliance-HPLC-System-1.jpg 147w," sizes="100vw" width="908" height="961"></p><div><div><h3><strong>Excellence through the years</strong></h3><p>The consistency of the Alliance HPLC System gives you <strong>exceptional confidence in your analytical results</strong>, day-to-day and year-to-year. Get <strong>accurate and reproducible results</strong> with <strong>Waters flagship HPLC technology</strong>, which provides <strong>consistent and reliable performance</strong>. Whether your Alliance HPLC System is newly manufactured, or you're working on one that is several years old, the Alliance HPLC System is a <strong>time-tested, dependable,</strong> and <strong>robust </strong>workhorse for your HPLC separations.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_HPLC_System_dfafc334c1.jpg" alt="Waters Alliance HPLC System: Comparison of the separation of carbamates using two different Alliance HPLC Systems, manufactured 7 years apart. The results are equivalent." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_HPLC_System_dfafc334c1.jpg 245w," sizes="100vw" width="1024" height="532"></p></div></div><div><div><h3><strong>Performance that exceeds your method requirements</strong></h3><p>The <strong>Alliance HPLC System</strong> ensures the <strong>reliability of your results</strong> via <strong>high injection precision</strong> and <strong>repeatable and accurate flow rates</strong>, resulting in <strong>easy peak identification</strong>, <strong>precise quantification</strong>, and <strong>exceptional retention time repeatability</strong>.</p><p>The integrated solvent delivery system features <strong>quaternary blending with a fixed delay volume</strong> for <strong>consistent and predictable results</strong>, regardless of system back pressure. The sample managers’ <strong>flexible injection capabilities</strong> cover a wide injection volume range (up to 2 mL) with <strong>excellent carryover performance</strong>, typically <strong>below 0.005%</strong>.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_HPLC_System_2_6dab43c365.jpg" alt="Waters Alliance HPLC System: USP method for Budesonide on the Alliance HPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_HPLC_System_2_6dab43c365.jpg 245w," sizes="100vw" width="1024" height="580"></p></div></div><div><div><h3><strong>Advancements in column chemistry and mass detection</strong></h3><p>The <strong>Alliance HPLC</strong> can leverage <strong>smaller particle size column chemistry</strong> to improve your laboratory's efficiency. With <strong>CORTECS 2.7 µm particle size columns</strong>, your lab will benefit from <strong>improvements in resolution</strong> and <strong>faster run times</strong> when transferring from a 5 µm size particle chemistry.</p><p>Further <strong>increase confidence in your data</strong> by adding <strong>mass confirmation with the ACQUITY QDa Detector</strong>, which is <strong>simple to use</strong> and can easily integrate into your existing workflow. <strong>Analysis in Empower 3 Software</strong> combines data from <strong>UV/Vis and mass spectrometry (MS)</strong> peaks to give <strong>mass information to each peak</strong> in the separation.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_HPLC_System_3_3711671b2e.jpg" alt="Waters Alliance HPLC System: Mass detection with the ACQUITY QDa Detector with UV detection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_HPLC_System_3_3711671b2e.jpg 245w," sizes="100vw" width="1024" height="550"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_HPLC_System_4_bf40981882.jpg" alt="Waters Alliance HPLC System: Mass detection with the ACQUITY QDa Detector with UV detection." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_HPLC_System_4_bf40981882.jpg 240w," sizes="100vw" width="1024" height="666"></p></div></div><div><div><h3><strong>Features and benefits</strong></h3><ul><li><strong>Quaternary solvent blending. </strong>Support isocratic and gradient applications with 11 unique gradient profile curves and blend up to 4 solvents in any proportion with Auto-Blend Technology. Compatible with solvent types such as reversed-phase, normal-phase, GPC, SEC, and IEX.</li><li><strong>Advanced solvent delivery capabilities. </strong>Serial flow path with pulse-free solvent delivery without the use of dampeners. Online solvent degassing and automated solvent compressibility compensation for accurate flow. Integrated seal wash for robust operation with all solvent types is standard.</li><li><strong>Optional, temperature-controlled environment.</strong> House up to 120 vials in an optional, temperature-controlled environment. Achieve low carryover with active and user programmable needle wash. Variable injection volumes from 0.1 to 100 μL standard, and up to 2 mL optional. Support STAT samples and auto-addition.</li><li><strong>Detection and column options. </strong>Get optional column heating, heating/cooling, or column switching capabilities. Detection capabilities include photodiode array, UV/Vis, fluorescence (FLR), refractive index (RI), evaporative light scattering (ELS), electrochemical, conductivity, and mass detection.</li><li><strong>Intuitive interface and software. </strong>Get instant access to system settings through the large LCD screen with simplified daily set-up with SystemPREP. Supports Empower, MassLynx, and 3<sup>rd</sup> party software packages.</li><li><strong>Compliance-ready. </strong>Supports Waters Empower-based automated system qualification tool (SQT), minimizing the time and cost of annual qualification with standards like GMP, GLP, ISO 17025, and USP &lt;1058&gt;.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_HPLC_System_2_47fe8951d2/Waters-Alliance-HPLC-System-2.jpg" alt="Waters Alliance HPLC System: Alliance HPLC System provides powerful functionality for all your application needs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_HPLC_System_2_47fe8951d2/thumbnail_Waters-Alliance-HPLC-System-2.jpg 161w," sizes="100vw" width="903" height="874"></p><h3><strong>Specifications</strong></h3><ul><li><strong>Maximum operating pressure</strong>: 5,000 psi</li><li><strong>Front panel display</strong>: Easy to use control panel</li><li><strong>Mobile phases</strong>: Up to 4 with Auto-Blend Technology</li><li><strong>Flow rate range</strong>: Up to 10 mL/min</li><li><strong>Sample carryover</strong>: ≤0.0025% (Caffeine) under specified conditions</li><li><strong>Sample compartment</strong>: 4–40 °C</li><li><strong>Pump options</strong>: Quaternary</li><li><strong>Solvent conditioning</strong>: Vacuum Degas</li><li><strong>Column capacity</strong>: Standard up to 3 columns</li><li><strong>Maximum sample capacity</strong>: 120</li><li><strong>Detector options</strong>:&nbsp;<ul><li>Tunable UV</li><li>Photo Diode Array</li><li>Fluorescence</li><li>Refractive Index</li><li>Evaporative Light Scattering</li><li>Electrochemical</li><li>Conductivity</li><li>Mass Spectrometry</li></ul></li><li><strong>External control</strong>:&nbsp;<ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/627">Empower Software</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/635">MassLynx Software</a></li></ul></li><li><strong>Physical specifications</strong>:<ul><li><strong>Width:</strong> 45.7 cm (18.0 in.) or 58.4 cm (23.0 in.) with column heater</li><li><strong>Height:</strong> 57.1 cm (22.5 in.)</li><li><strong>Depth:</strong> 57.1 cm (22.5 in.) or 64.8 cm (25.5 in.) with sample heater/cooler</li></ul></li></ul></div></div></div></div></div></div></div></div>

Waters Alliance iS Bio HPLC System

Alliance iS Bio HPLC combines MaxPeak HPS Technology, bio-inert separations, reduced errors, and enhanced reproducibility for biopharma QC workflows.

<p><strong>The Waters Alliance iS Bio HPLC System is an advanced liquid chromatography platform specifically designed for biopharmaceutical quality control laboratories. Combining exceptional bio-inertness, intuitive operation, and intelligent automation tools, the system helps reduce human error, improve productivity, and ensure consistent analytical performance.</strong></p><p>Featuring <strong>MaxPeak™ High Performance Surfaces (HPS) Technology</strong>, the Alliance iS Bio minimizes unwanted interactions between analytes and metallic flow path surfaces. This improves analyte recovery, sensitivity, reproducibility, and retention time consistency while reducing system conditioning requirements. The technology is particularly beneficial for oligonucleotides, phosphopeptides, monoclonal antibodies, and other metal-sensitive biomolecules.</p><p>The system integrates intuitive touchscreen operation, guided workflows, RFID-enabled column tracking, and advanced compliance-support tools. Combined with corrosion-resistant construction materials and robust performance under challenging operating conditions, the Alliance iS Bio HPLC System is an ideal platform for modern biopharma QC laboratories.</p><h3><strong>Key Features</strong></h3><ul><li>MaxPeak™ High Performance Surfaces (<strong>HPS) Technology</strong></li><li>Highly <strong>bio-inert sample flow path</strong></li><li>Reduced analyte-surface interactions</li><li>Improved analyte recovery and sensitivity</li><li>Enhanced reproducibility and retention time consistency</li><li>Up to 40% reduction in human errors</li><li>Intuitive touchscreen operation</li><li>Guided workflows</li><li><strong>RFID-enabled eConnect column</strong> tracking</li><li><strong>Intelligent Method Translator App</strong> (iMTA)</li><li>Automated column history tracking</li><li>Empower Audit Trail functionality</li><li><strong>waters_connect™</strong> System Monitoring</li><li>Integrated maintenance and troubleshooting tools</li><li><strong>Carryover ≤ 0.002%</strong></li><li>Robust operation across extreme temperature conditions</li><li>Tool-free finger-tight fittings</li></ul><h3><strong>Technical Specifications</strong></h3><h4><strong>Chromatography System</strong></h4><ul><li><strong>System type: </strong>HPLC</li><li><strong>Pump configuration: </strong>Quaternary</li><li><strong>Injection type:</strong> Flow-through needle</li><li><strong>Solvent conditioning:</strong> Passive pre-heating</li></ul><h4><strong>Operating Parameters</strong></h4><ul><li><strong>Maximum operating pressure:</strong> 12,000 psi</li><li><strong>Flow rate range:</strong> 0.001–10.000 mL/min</li><li><strong>pH range:</strong> 1–13</li><li><strong>Carryover:</strong> ≤ 0.002% (caffeine, UV conditions)</li></ul><h4><strong>Sample Management</strong></h4><ul><li><strong>Sample compartment temperature:</strong> 4–40 °C</li><li><strong>Maximum sample capacity:</strong><ul><li>48-position 2.0 mL vial holder</li><li>54-position 2.0 mL vial holder</li><li>96-well plate</li><li>384-well plate</li><li>48-position 0.65 mL microcentrifuge tube plate</li><li>24-position 1.5 mL microcentrifuge tube plate</li></ul></li></ul><h4><strong>Column Management</strong></h4><ul><li>RFID column tracking</li><li>eConnect Waters Column support</li><li><strong>Capacity:</strong><ul><li>One column up to 300 mm length</li><li>Up to 8 mm internal diameter</li></ul></li></ul><h4><strong>Detector Options</strong></h4><ul><li>PDA Detector</li><li>TUV Detector</li></ul><h4><strong>Software and Informatics</strong></h4><ul><li>Empower™ Chromatography Data System (CDS)</li><li>Intelligent Method Translator App (iMTA)</li><li>waters_connect™ Monitoring</li><li>Audit Trail functionality</li><li>Automated column history tracking</li></ul><h4><strong>Construction</strong></h4><ul><li>Inert sample flow path with MaxPeak HPS Technology</li><li>Corrosion-resistant bio-compatible materials</li><li>High-salt tolerant design</li></ul><h4><strong>Dimensions and Weight</strong></h4><ul><li><strong>Width: </strong>49.9 cm</li><li><strong>Height: </strong>74.2 cm</li><li><strong>Depth:</strong> 63.5 cm</li><li><strong>Weight:</strong> 72.5 kg</li></ul><h4><strong>Typical Applications</strong></h4><ul><li>Biopharmaceutical QC</li><li>Monoclonal antibodies (mAbs)</li><li>Oligonucleotide analysis</li><li>Phosphopeptide analysis</li><li>Peptide mapping</li><li>Size exclusion chromatography (SEC)</li><li>Bioseparations</li><li>Pharmaceutical QC</li><li>Regulatory testing laboratories</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SEC_separation_of_a_USP_m_Ab_System_Suitability_Solution_on_a_legacy_HPLC_system_and_the_Alliance_i_S_Bio_HPLC_System_with_a_Bio_Suite_Diol_SEC_Column_851dc3a90f.jpg" alt="Waters: SEC separation of a USP mAb System Suitability Solution on a legacy HPLC system and the Alliance iS Bio HPLC System with a BioSuite Diol SEC Column." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SEC_separation_of_a_USP_m_Ab_System_Suitability_Solution_on_a_legacy_HPLC_system_and_the_Alliance_i_S_Bio_HPLC_System_with_a_Bio_Suite_Diol_SEC_Column_851dc3a90f.jpg 234w," sizes="100vw" width="1024" height="682"></p>

Waters Alliance iS HPLC System

Waters™ delivers a reliable partner for QC labs. The Alliance™ iS HPLC System enhances efficiency, reduces errors, and simplifies workflows - your new essential lab ally.

<h3><strong>The Alliance iS HPLC System didn’t start in our lab.</strong></h3><p class="cke-user_style-important"><strong>It started in yours.</strong></p><p><strong>Pharmaceutical Quality Control (QC)</strong> labs face relentless demand to <strong>do more with less</strong> amid higher staff turnover, growing levels of sample complexity, and the drive to reduce errors.</p><p>With regulatory scrutiny and faster time to market fueling these intense demands, the need for <strong>simplicity has never been greater</strong>.</p><p>Waters™ understands the challenges you face and is ready to deliver a true ally for your laboratory. With capabilities developed specifically for the QC laboratory, the <strong>Alliance™ iS HPLC System</strong> will quickly become your favorite ally.</p><ul style="list-style-type:disc;"><li>Intuitively prevents up to 40% of common errors*</li><li>Provides fast, simple guidance at the point of need</li><li>Boosts productivity and capacity through effective resource utilization and error-free operation</li><li>Drives workflow efficiencies and quality improvements</li><li>Improves data integrity</li></ul><p>*Based on Waters’ market research in 2022, surveying 56 global Pharma QC labs running &gt;25 systems</p><h3><strong>Common challenges facing the QC lab</strong></h3><p class="cke-user_style-important"><strong>What you are telling us</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_i_S_HPLC_System_cc30c5f925.jpg" alt="Waters Alliance iS HPLC System - What you are telling us" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_i_S_HPLC_System_cc30c5f925.jpg 245w," sizes="100vw" width="2038" height="1132"></p><h3><strong>Meet your new lab ally</strong></h3><p>Intuitive and reliable, the <strong>Alliance iS HPLC System</strong> is designed to work seamlessly with <strong>Empower™ Software</strong> and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720007883en_41b7055d98/720007883en.pdf"><strong>eConnect™ Column Tag Technology</strong></a>, unlocking unique features and functionality that eliminate the need to choose between simplicity, efficiency, and quality.</p><h3><strong>Step-change the performance of your lab</strong></h3><p class="cke-user_style-important"><strong>Achieve First Time Quality (FTQ) with the Alliance iS HPLC System</strong></p><p>The <strong>Alliance iS HPLC System</strong> supports FTQ by reducing the risk of common errors and improving the reliability of analytical measurements. Your lab can <strong>reduce reagents and consumables waste</strong>, and automated system checks help to meet critical error reduction KPIs. The increase in operational efficiency and productivity will save time and money and boost the capacity of your lab.**</p><p>With ease of use at the heart of the user experience, the <strong>Alliance iS HPLC System</strong> identifies potential errors earlier, reducing the number of retests and failure investigations. This supports a Quality culture resulting not only in safer products critical to human health and well-being, but also an improved working environment, directly impacting employee retention and motivation.**</p><p>**vs the Alliance HPLC System</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_i_S_HPLC_System_2_44577b6737.jpg" alt="Waters Alliance iS HPLC System with bottles connected." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_i_S_HPLC_System_2_44577b6737.jpg 234w," sizes="100vw" width="1024" height="682"></p><h3><strong>The System: intuitively simple</strong></h3><h5><strong>System Status Lighting and Solvent Tubing Clips&nbsp;</strong></h5><p>Status lighting provides you with a clear visual indication of the system status (in use, idle etc), while the color-coded solvent tubing clips ensure the tubing remains organized to enable tracing from instrument to the solvent bottle flags.</p><h5><strong>Made for Empower</strong></h5><p><strong>Waters Empower Software Suite</strong> helps your entire laboratory operate better, with the industry’s most advanced data acquisition, management, processing, analytics, and reporting capabilities. Empower Software provides you an environment where your records are traceable, so you always have full control of your most critical asset - your data.</p><h5><strong>waters_connect™ System Monitoring&nbsp;</strong></h5><p>Maximize lab productivity - utilize resources more effectively with real-time lab management from anywhere, anytime. System Monitoring provides intuitive and centralized access to detailed Empower Software instrument information via the waters_connect Cloud Platform, without compromising security or compliance.</p><h5><strong>Intelligent Method Translator App&nbsp;</strong></h5><p>Working with Empower Software, the iMTA enables you to create an Alliance iS HPLC System Instrument Method directly from a Waters or other third-party LC system. Basic chromatographic conditions are automatically transferred for you, and the new Empower instrument method can be reported, providing traceability to the original method for compliance.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_i_S_HPLC_System_with_bottles_connected_and_the_doors_open_ff4184918a.jpg" alt="Waters Alliance iS HPLC System with bottles connected and the doors open." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_i_S_HPLC_System_with_bottles_connected_and_the_doors_open_ff4184918a.jpg 234w," sizes="100vw" width="1024" height="682"></p><h3><strong>Say ‘hi’ to your lab ally</strong></h3><h5><strong>Intuitive touchscreen&nbsp;</strong></h5><p>An easy-to-use, advanced touchscreen integrates with Empower Software and provides easy-to-follow steps. Pre-run checks for common errors such as missing vials, incorrect column, and qualification/preventative maintenance requirements enable you to catch these mistakes before they result in a sample analysis failure, preventing re-runs.</p><h5><strong>Help at your fingertips&nbsp;</strong></h5><p>The Alliance iS HPLC System support journey delivers smart on-board technology that digitally brings you straight to the information you need at the new Waters Help Center. Train and troubleshoot with ease, knowing that Waters will support you at every stage.</p><h5><strong>Smart column capabilities&nbsp;</strong></h5><p>Ensure complete column traceability and facilitate post-run troubleshooting with the eConnect HPLC Columns. Ready-to-use from the box, eConnect columns are securely fitted with an NFC-enabled eConnect device tag, ensuring they are always automatically identified, verified, and tracked by the system. These second-generation smart columns propel today’s QC laboratory toward an entirely digital platform and away from manual logbooks.</p><h3><strong>Enjoy stress-free analysis</strong></h3><p>The Alliance iS HPLC System keeps you and your lab stress-free and running smoothly with an intuitive touchscreen that supports the productivity and efficiency of your lab.</p><ul><li>Start your day easily with a system guided start-up.</li><li>Pre-run checks to minimize sample analysis failures due to human error.</li><li>When issues arise, Alliance iS HPLC System provides you with information about what needs attention.</li><li>Guided workflows provide step-by-step guidance on how to resolve common issues.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_i_S_HPLC_System_2_0e64835283.jpg" alt="Waters Alliance iS HPLC System - Enjoy stress-free analysis." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_i_S_HPLC_System_2_0e64835283.jpg 204w," sizes="100vw" width="1388" height="1063"></p><h3><strong>Built to perform, time after time</strong></h3><h4><strong>Intuitive innovation</strong></h4><p>Developed with the singular goal of providing innovation that solves the problems that matter in the QC lab, the Alliance iS HPLC System includes a range of new and unique features.&nbsp;</p><ul><li>New electronics to enable the Alliance iS HPLC System smart features (including advanced touchscreen, eConnect Column Tag Technology, and unparalleled interactivity with Empower Software)</li><li>Redesigned check valves and mixer for increased performance and robustness</li><li>Faster detector warm-up time and reduced drift to ensure you’re up and running quickly, for as long as your analysis requires</li><li>Robust performance when operating at the extremes of the Column Heater Cooler and Sample Manager temperature ranges</li><li>Class leading carryover performance and injection precision</li></ul><figure class="image"><img style="aspect-ratio:1561/740;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_i_S_HPLC_System_3_9aab20fd13.jpg" alt="Waters Alliance iS HPLC System - Overlay of 42 injections of gradient performance mix over 44 hours demonstrated RT repeatability of 0.5% RSD on the Alliance iS HPLC System" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_i_S_HPLC_System_3_9aab20fd13.jpg 245w," sizes="100vw" width="1561" height="740"></figure><h3><strong>Alliance iS HPLC System gives you so much more</strong></h3><p class="cke-user_style-important"><strong>Unlock new potential with Empower Software</strong></p><p><strong>The Alliance iS HPLC System works exclusively with our </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720008057en_af76145fe7/720008057en.pdf"><strong>Empower Software</strong></a><strong> version 3 Feature Release 4 </strong>or later, with fully enabled and differentiated features, starting with eConnect Technology. Empower Software elevates your quality, your business, and your lab.</p><ul style="list-style-type:disc;"><li>Intuitive method matching</li><li>Bi-directional touchscreen interactivity</li><li>Column tracking and history</li><li>Enhanced instrument traceability</li><li>Pre-run checks</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_i_S_HPLC_System_4_430a084194.jpg" alt="Waters Alliance iS HPLC System gives you so much more." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_i_S_HPLC_System_4_430a084194.jpg 245w," sizes="100vw" width="1385" height="674"></p><h3><strong>Help at your fingertips</strong></h3><p class="cke-user_style-important"><strong>From onscreen to onsite, we’re there for you.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Alliance_i_S_HPLC_System_5_df4f1467ed.jpg" alt="Waters Alliance iS HPLC System - From onscreen to onsite, we’re there for you." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Alliance_i_S_HPLC_System_5_df4f1467ed.jpg 245w," sizes="100vw" width="1550" height="910"></p><p>Maintain peak performance, minimize unexpected downtime, and ensure regulatory compliance of your AllianceTM iS HPLC System by partnering with Waters Service. Waters FlexCHOICETM Full-Service Plan for Regulated Laboratories includes:</p><ul style="list-style-type:disc;"><li>Yearly Waters Performance Maintenance</li><li>Priority* remote and onsite service including all parts, labor and travel</li><li>Full suite of compliance services</li><li>Discounts on future hardware upgrades and user training</li><li>Optional coverage available:<ul><li>Additional Performance Maintenance visits</li><li>Two business day on-site response time**</li></ul></li></ul><p>Priority over non-contract customers **May not be available in all areas</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_The_first_system_designed_for_Empower_the_Alliance_i_S_HPLC_System_features_intuitive_simplicity_built_specifically_for_QC_laboratories_112259fad6.jpg" alt="Waters Alliance iS HPLC System - The first system designed for Empower, the Alliance iS HPLC System features intuitive simplicity built specifically for QC laboratories." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_The_first_system_designed_for_Empower_the_Alliance_i_S_HPLC_System_features_intuitive_simplicity_built_specifically_for_QC_laboratories_112259fad6.jpg 234w," sizes="100vw" width="1024" height="682"></p>

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