<p><strong>Per- and polyfluoroalkyl substances (PFAS) have earned the moniker "forever chemicals" due to their extreme persistence in the environment. Their detection is crucial for protecting public health and the environment as these synthetic compounds continue to pose significant challenges worldwide. This comprehensive exploration examines why PFAS detection matters, current detection methods, and future directions in addressing PFAS contamination.</strong></p><h3><strong>Understanding PFAS and Their Health Impacts</strong></h3><p>PFAS are a class of synthetic organic compounds with diverse structures, properties, and uses. Despite their diversity, all PFAS contain perfluoroalkyl moieties that are extremely resistant to environmental and metabolic degradation [1]. This high persistence is a defining characteristic that makes PFAS management particularly challenging.</p><h3><strong>Documented Health Effects</strong></h3><p>Current scientific research has linked PFAS exposure to numerous adverse health outcomes:</p><ul><li>Reproductive effects including decreased fertility and increased high blood pressure in pregnant women [8]</li><li>Developmental effects in children such as low birth weight, accelerated puberty, and behavioral changes [8]</li><li>Reduced immune system function, including decreased vaccine response</li><li>Interference with natural hormones and endocrine disruption</li><li>Increased cholesterol levels and obesity risk</li><li>Increased risk of several cancers, including prostate, kidney, testicular, oral cavity/pharynx, brain, and endocrine cancers [3]</li></ul><p>A 2023 study linked PFAS-contaminated water with up to a <strong>33% increase in cancer incidence</strong> in affected areas [3]. The study compared regions with elevated PFAS levels to cancer data from the National Cancer Institute, finding higher incidences of various cancers after controlling for factors like smoking and obesity.</p><h3><strong>Environmental Persistence and Contamination</strong></h3><p>The extreme persistence of PFAS represents a fundamental concern for environmental management. PFAS are considered the most environmentally persistent substances among organic chemicals [7]. This persistence means that even if all PFAS uses were immediately phased out, existing contamination would continue to affect ecosystems and water supplies for generations.</p><p>A 2023 study at a military fire training site estimated that PFAS contamination will persist for centuries without remediation [7]. The research found that terminal PFAS still exceed regulatory limits by 2000-fold decades after aqueous film-forming foam (AFFF) use ceased, with precursors accounting for around half of the extractable organofluorine across years.</p><h3><strong>Current Detection Methods</strong></h3><h4><strong>EPA Standardized Methods</strong></h4><p>The<strong> Environmental Protection Agency (EPA</strong>) has developed several standardized methods for PFAS detection, each with specific applications and capabilities:</p><h5><strong>EPA Method 533</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/epa-533-drying-down-pfas-samples-after-spe"><strong>EPA Method 533</strong></a> focuses on "short chain" PFAS with carbon chain lengths of<strong> C4 to C12</strong>. Released in December 2019, this method complements Method 537.1 and includes several key features:</p><ul><li>Uses isotope dilution quantification approach with extracted internal standards, which reduces overall analytical uncertainty</li><li>Employs weak anion exchange solid phase extraction cartridges as opposed to polystyrene divinylbenzene used in 537.1</li><li>Can analyze 25 PFAS compounds, including additional short-chain PFAS that were not apart of Method 537.1</li><li>Allows for longer holding times: 28 days to extraction and 28 days to analyze (compared to 14 days to extraction in Method 537.1)</li><li>Uses ammonium acetate as a preservative (versus Trisma in Method 537.1) [1]</li></ul><h5><strong>EPA Method 537/537.1</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/sample-concentration-epa-method-537-pfas-determination"><strong>EPA Method 537 </strong></a>was first published in 2009 for the determination of 14 PFAS in drinking water [6]. It was later updated to Method 537.1 in 2018 (with editorial updates in 2020) to expand its capabilities:</p><ul><li>Uses<a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/what-is-solid-phase-extraction-spe"><strong> solid phase extraction (SPE) </strong></a>and<a target="_blank" rel="noopener noreferrer" href="https://www.organomation.com/preparing-samples-for-lc-ms/ms-analysis"><strong> liquid chromatography/tandem mass spectrometry (LC-MS/MS)</strong></a></li><li>Method 537.1 expanded detection to 18 PFAS compounds, including HFPO-DA (one component of GenX technology)</li><li>Utilizes styrene-divinylbenzene (SDVB) media for extraction</li><li>Requires samples to be extracted within 14 days and analyzed within 28 days</li><li>Methanol is used to elute the extracts. This should then be reconstituted with a 96:4 methanol:water mixture [1]</li></ul><p>Together, Methods 533 and 537.1 can detect a total of <strong>29 unique PFAS compounds in drinking water </strong>and are approved for monitoring under the fifth Unregulated Contaminant Monitoring Rule (UCMR 5) and the PFAS National Primary Drinking Water Regulation [1] [3].</p><h5><strong>EPA Method 1633</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/preparing-diverse-sample-matrices-for-epa-method-1633"><strong>EPA Method 1633</strong></a>, released in 2024, represents a significant advancement in PFAS analysis:</p><ul><li>Developed for the analysis of <strong>40 PFAS compounds </strong>in various matrices, including surface water, groundwater, wastewater, soil, biosolids, sediment, landfill leachate, and fish tissue</li><li>Expands beyond the drinking water focus of Methods 533 and 537.1</li><li>Provides a standardized approach that may replace modified drinking water methods (i.e., modified EPA 537) that were previously used for non-drinking water matrices [1]</li></ul><h4><strong>Conventional Analytical Techniques</strong></h4><p>The gold standard for PFAS analysis involves chromatographic techniques coupled with mass spectrometry:</p><ul><li>Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) is the most widely used method for PFAS analysis, offering high sensitivity and specificity [1]</li><li>High-Resolution Mass Spectrometry (HRMS) allows for rapid detection and identification of PFAS species at very low concentrations [5]</li></ul><p>These techniques can detect PFAS at parts per trillion (ppt) levels, which is essential given the low concentrations at which PFAS can cause health effects [1]. However, these methods require sophisticated equipment, specialized training, and significant financial resources, making them inaccessible in many regions, particularly in developing countries.</p><h4><strong>Emerging Detection Technologies</strong></h4><p>Several innovative approaches are being developed to address the limitations of conventional methods:</p><ul><li>Electrochemical biosensors using modified electrodes have shown promise for selective PFAS detection with low detection limits [2]</li><li>Surface-Enhanced Raman Spectroscopy (SERS) uses metallic nanoparticles and can be made into a portable method for on-site testing [1]</li><li>Molecular imprinted polymers (MIPs) offer thermal and physical stability, reusability, and low-cost synthesis for PFAS detection [6]</li></ul><p>These emerging technologies aim to provide more accessible, field-deployable, and cost-effective alternatives to traditional laboratory-based methods.</p><h3><strong>Challenges in PFAS Detection</strong></h3><h4><strong>Analytical Challenges</strong></h4><p>Detecting PFAS presents several technical challenges:</p><ul><li>The ubiquity of PFAS in laboratory equipment and accessories can lead to background contamination when measuring at parts-per-trillion levels [1]</li><li>Many components in liquid chromatographs, mass spectrometers, and solid-phase extraction systems contain materials that can leach PFAS compounds [9]</li><li>The vast number of PFAS (thousands of compounds) makes comprehensive detection difficult, as analytical standards are only available for a small subset [1]</li><li>Complex matrices like food and environmental samples require sophisticated sample preparation techniques</li></ul><h4><strong>Global Disparities in Detection Capabilities</strong></h4><p>Access to PFAS detection technology varies significantly worldwide:</p><ul><li>Many developing regions, particularly in Africa, lack adequately equipped laboratories with the necessary instrumentation to detect and measure PFAS</li><li>There is often a scarcity of qualified professionals with experience in PFAS analysis in these regions</li><li>The high cost of acquiring and maintaining sophisticated analytical instruments presents a substantial obstacle to conducting extensive PFAS investigations in resource-limited settings [1]</li><li>Competing priorities such as infectious diseases, poverty, and food security often divert attention and resources from environmental pollutants like PFAS</li></ul><h4><strong>Regulatory Landscape and Compliance</strong></h4><p>The regulatory framework for PFAS is evolving rapidly worldwide:</p><ul><li>The EPA finalized Section 8(a)(7) of the Toxic Substances Control Act in September 2024, requiring manufacturers and importers to report on their use of PFAS since 2011 [10]</li><li>Electronic reporting for PFAS will open on July 11, 2025, with final reporting deadlines in 2026</li><li>Companies must report detailed information including chemical identity, production volumes, industrial and consumer products, worker exposure, and disposal methods [10]</li></ul><p>These regulations are driving demand for more effective and accessible PFAS detection methods to ensure compliance and protect public health.</p><h3><strong>Future Directions in PFAS Detection</strong></h3><h4><strong>Technological Advancements</strong></h4><p>The future of PFAS detection lies in developing more accessible, sensitive, and comprehensive methods:</p><ul><li>Non-targeted screening methods that can identify unknown PFAS compounds without requiring analytical standards for each specific compound</li><li>Total fluorine analysis techniques like Adsorbable Organic Fluorine and Total Oxidizable Precursor Assay that can quantify the presence of fluorine atoms within a sample [1]</li><li>Portable sensors and field-deployable technologies that enable on-site monitoring and rapid response to contamination events</li><li>AI-driven solutions that can improve data analysis and interpretation for complex PFAS mixtures</li></ul><h4><strong>Collaborative Approaches</strong></h4><p>Addressing the global challenge of PFAS detection requires coordinated efforts:</p><ul><li>International partnerships between researchers in developed and developing regions to enhance expertise and capacity building</li><li>Standardization of analytical methods to ensure consistency and comparability of results across different laboratories and regions</li><li>Open data sharing and collaboration across countries to leverage collective knowledge and resources</li><li>Integration of PFAS monitoring into broader environmental and public health surveillance systems</li></ul><h3><strong>Conclusion</strong></h3><p>The detection of PFAS is not merely a technical challenge but a critical public health and environmental imperative [1]. As these "forever chemicals" continue to persist in our environment, developing effective, accessible, and comprehensive detection methods becomes increasingly important. By advancing detection technologies, strengthening regulatory frameworks, and fostering global collaboration, we can better understand, monitor, and ultimately mitigate the impacts of PFAS contamination.</p><p>The EPA's standardized methods (533, 537.1, and 1633) provide crucial tools for monitoring PFAS in various environmental matrices, but continued innovation is needed to address the full spectrum of PFAS compounds and to make detection more accessible globally [1] [6]. The path forward requires sustained investment in research and development, capacity building in resource-limited settings, and a commitment to environmental justice that ensures all communities have access to the tools and information needed to protect themselves from PFAS exposure. Only through such comprehensive approaches can we hope to address the complex challenges posed by these persistent pollutants.</p><p><span>Be sure to check out our other installments of our&nbsp;</span><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/introducing-our-environmental-contaminants-series-making-sense-of-the-alphabet-soup"><span>Environmental Contaminant Series</span></a><span>&nbsp;covering&nbsp;</span><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/the-importance-of-detecting-persistent-organic-pollutants-pops"><span>Persistent Organic Pollutants (POPs)</span></a><span>,&nbsp;</span><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/the-importance-of-detecting-polycyclic-aromatic-hydrocarbons-pahs"><span>Polycyclic Aromatic Hydrocarbons (PAHs)</span></a><span>,&nbsp;</span><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/the-importance-of-detecting-semi-volatile-organic-compounds-svocs"><span>Semi-Volatile Organic Compounds (SVOCs)</span></a><span>, and </span><a target="_blank" rel="noopener noreferrer" href="https://blog.organomation.com/blog/the-importance-of-detecting-polychlorinated-biphenyls-pcbs"><span>Polychlorinated Biphenyls (PCBs)</span></a><span>.</span></p>

The Importance of Detecting Per- and Polyfluoroalkyl Substances (PFAS)

<p><strong>Scientists at CEITEC Brno University of Technology have developed microscopic robots that could one day accelerate beer production and simplify food quality control. The magnetically controlled biobots can actively mix liquids during fermentation, speed up the process, and separate themselves from the finished product once fermentation is complete. The results of the research, carried out by Professor Martin Pumera’s team, were published in the journal </strong><i><strong>ACS Nano</strong></i><strong>.</strong></p><p><i>“When people hear the word ‘robot’ today, they often imagine an electronic device. In reality, however, a robot can be any system capable of autonomously performing a specific task,” </i>explains <strong>Martin Pumera of CEITEC Brno University of Technology</strong>.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Mikroroboti_z_CEITEC_mohou_urychlit_vyrobu_piva_i_kontrolu_kvality_potravin_7d9ff82875.jpeg" alt="CEITEC BUT/Jakub Rozboud: CEITEC Microrobots Could Speed Up Beer Production and Food Quality Control: Martin Pumera in his lab at CEITEC BUT." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Mikroroboti_z_CEITEC_mohou_urychlit_vyrobu_piva_i_kontrolu_kvality_potravin_7d9ff82875.jpeg 235w," sizes="100vw" width="1600" height="1067"></p><p>The research team created so-called biobots—biohybrid microrobotic capsules containing brewer’s yeast and magnetic nanoparticles. When the yeast produces carbon dioxide during fermentation, the resulting bubbles cause the biobots to move up and down through the liquid. This naturally mixes the contents of the vessel and makes fermentation more efficient. Compared with conventional static particles, the biobots can therefore accelerate chemical processes that are important in food production.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Mikroroboti_z_CEITEC_mohou_urychlit_vyrobu_piva_i_kontrolu_kvality_potravin_4_af5fd4611f.jpg" alt="CEITEC BUT/Martin Pumera's archive: CEITEC Microrobots Could Speed Up Beer Production and Food Quality Control: Hematite microrobot (image for illustrative purposes)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Mikroroboti_z_CEITEC_mohou_urychlit_vyrobu_piva_i_kontrolu_kvality_potravin_4_af5fd4611f.jpg 209w," sizes="100vw" width="1600" height="1192"></p><p>Another major advantage of the technology is that the biobots can be easily separated from the finished beverage. Thanks to the magnetic particles they contain, they can be controlled with a magnetic field and removed once fermentation is complete, eliminating the need for additional filtration. The researchers also demonstrated that the biobots can be reused over several production cycles.</p><p><i>“Unlike conventional static particles, microrobots actively move throughout the entire volume of the liquid, allowing them to cover a larger area and make the whole process more efficient. At the same time, they also facilitate the automation of production,”</i> adds Martin Pumera. The team is currently discussing potential applications of the technology with a major European brewery.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Mikroroboti_z_CEITEC_mohou_urychlit_vyrobu_piva_i_kontrolu_kvality_potravin_3_e8e5a3a55a.jpg" alt="CEITEC BUT/Martin Pumera's archive: CEITEC Microrobots Could Speed Up Beer Production and Food Quality Control: g-C₃N₄ microrobots (image for illustrative purposes)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Mikroroboti_z_CEITEC_mohou_urychlit_vyrobu_piva_i_kontrolu_kvality_potravin_3_e8e5a3a55a.jpg 158w," sizes="100vw" width="1476" height="1460"></p><p>Martin Pumera’s group has long been exploring the use of microrobots and advanced materials in the food industry. In the past, for example, the team developed a flexible sensor for smart packaging capable of detecting spoiled fish. The new biobots therefore represent another step towards more advanced methods of food quality and safety control.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Mikroroboti_z_CEITEC_mohou_urychlit_vyrobu_piva_i_kontrolu_kvality_potravin_2_75c7fed958.jpeg" alt="CEITEC BUT/ACS Nano: CEITEC Microrobots Could Speed Up Beer Production and Food Quality Control: ACS Nano - cover featuring article by Martin Pumera et al." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Mikroroboti_z_CEITEC_mohou_urychlit_vyrobu_piva_i_kontrolu_kvality_potravin_2_75c7fed958.jpeg 117w," sizes="100vw" width="1600" height="2128"></p>

 CEITEC Microrobots Could Speed Up Beer Production and Food Quality Control 

<p><strong>In this episode of ‪ChromatographyTalk‬, ‪Organomation‬ General Manager David Oliva sits down with Michelle Taylor, Editor-in-Chief of LabCompare, to go behind the scenes of their groundbreaking two-part documentary series on PFAS (per- and polyfluoroalkyl substances).</strong></p><p>The conversation kicks off with a startling revelation: many of the world’s leading PFAS experts have completely stopped eating seafood due to high contamination levels discovered during testing. From there, we dive deep into the technical and regulatory challenges facing today's analytical laboratories.</p><h4><strong>In this episode, we discuss:</strong></h4><ul><li><strong>The Laboratory Throughput Crisis:</strong> Why labs are currently "overwhelmed with samples" and how tiered testing—using single quad instruments for screening before moving to triple quads—is helping to manage capacity without sacrificing sensitivity.</li><li><strong>The Battle Against Background Contamination:</strong> Why PTFE-free (PFAS-free) laboratory equipment is no longer optional when trying to detect "forever chemicals" at the parts-per-trillion and parts-per-quadrillion levels.</li><li><strong>Complex Matrices (Seafood &amp; Packaging): </strong>The technical hurdles of analyzing high-lipid samples like fish and the rising demand for migration testing as states like Rhode Island begin to phase out PFAS in food packaging.</li><li><strong>Global Regulatory Shifts:</strong> A look at the "dual compliance model" required for labs supporting international exporters, especially with the European Union’s stricter enforcement compared to the U.S. monitoring approach.</li><li><strong>The "Grand Challenge" of Unknowns: </strong>How EPA Method 1621 (Total Organic Fluorine) is helping labs close the mass balance gap and why the next generation of bench chemists must embrace *AI and data science* to manage the massive datasets generated by high-resolution mass spectrometry.</li><li><strong>PFAS Destruction vs. Remediation:</strong> &nbsp;The promising (and problematic) methods for breaking the carbon-fluorine bond, and why incineration may inadvertently be spreading shorter-chain PFAS into the air.</li></ul><p>Whether you are a lab manager looking to future-proof your workflow or a chemist navigating the ever-evolving landscape of EPA Method 1633, this episode provides essential insights into the most pressing environmental crisis of our time.</p><h3><strong>Video Transcription</strong></h3><h4><strong>Michelle Taylor: PFAS Testing Is Becoming a Much Bigger Analytical Challenge</strong></h4><p><strong>PFAS analysis has rapidly evolved from a specialized environmental testing problem into a much broader challenge involving drinking water, food, packaging, laboratory contamination, international regulation, data analysis, and even destruction technologies. In an interview with Michelle Taylor, Editor-in-Chief of Labcompare, the discussion explored what laboratories are learning from the latest PFAS research—and why the analytical demands are likely to continue growing.</strong></p><p>Taylor leads editorial content for <strong>Labcompare</strong>, an online resource covering analytical laboratory instrumentation, products, and scientific developments. Among the projects she considers particularly important are two long-form documentaries produced by Labcompare examining per- and polyfluoroalkyl substances (PFAS), first through drinking water and subsequently through food.</p><p>The documentaries brought together subject-matter experts working across PFAS analysis and exposed some of the practical realities laboratories now face: rapidly changing regulations, extremely low detection limits, complex matrices, contamination risks, and sample volumes that can overwhelm laboratory capacity.</p><h4><strong>From drinking water to seafood</strong></h4><p>One of the most memorable findings for Taylor came during production of the second documentary, which focused on PFAS in food.</p><p>Several experts interviewed for the project told her they had stopped eating seafood, in some cases many years earlier, because of concerns surrounding contamination. For Taylor, the consistency and immediacy of those responses illustrated how closely analytical research can intersect with everyday decisions.</p><p>Seafood also demonstrates why PFAS testing can become analytically difficult.</p><p>Fish and other high-lipid samples require effective extraction to release the analytes, followed by rigorous cleanup to remove fats. According to the experts Taylor interviewed, the cleanup step is particularly critical because residual lipids can damage LC columns and create severe matrix interferences.</p><p>Laboratories are therefore using approaches such as enhanced matrix removal and carbon- or silica-based sorbents to reduce the lipid content of extracts before instrumental analysis. The goal is not simply obtaining sufficient recovery, but producing an extract clean enough to maintain analytical performance and laboratory throughput.</p><h4><strong>How laboratories are handling the PFAS sample burden</strong></h4><p>The analytical sensitivity now required for PFAS testing presents another challenge: laboratories may need to measure compounds at <strong>parts-per-trillion levels</strong> while processing growing numbers of samples.</p><p>Automation has therefore become increasingly important, particularly during sample preparation, where repetitive manual procedures can otherwise create a major bottleneck.</p><p>Taylor also described a <strong>tiered analytical strategy</strong> being used by some laboratories. A less demanding measurement can first be used for screening, while samples generating a positive result are subsequently transferred to a more sensitive platform, such as triple-quadrupole mass spectrometry, for confirmation and quantitation.</p><p>This type of workflow can help laboratories concentrate their highest-performance instrumentation on the samples that actually require it rather than applying the most intensive analysis to every incoming sample.</p><p>The broader principle is likely to become increasingly important as PFAS testing expands: laboratories must find ways to increase throughput without sacrificing the sensitivity demanded by regulatory methods.</p><h4><strong>When the laboratory itself becomes a source of PFAS</strong></h4><p>At trace concentrations, contamination introduced during sample preparation or measurement can become comparable to the concentration being determined.</p><p>This makes the materials used throughout the analytical workflow particularly important.</p><p>Taylor emphasized that PFAS laboratories must maintain continuous awareness of potential contamination from <strong>solvents, water, tubing, containers, and other laboratory components</strong>. Equipment containing fluoropolymers such as PTFE may be problematic in certain PFAS workflows because background contamination can contribute to false positives or elevated blanks.</p><p>As detection limits move lower, dedicated PFAS-compatible or fluoropolymer-free laboratory equipment becomes increasingly valuable.</p><p>Taylor described manufacturers offering clearly identified PFAS-free configurations as an important response to this problem. Rather than forcing laboratories to investigate the material composition of each component individually, dedicated configurations can simplify method implementation and reduce uncertainty about potential background sources.</p><p>For laboratories already dealing with demanding workflows and large sample loads, eliminating potential contamination before it occurs can save considerable troubleshooting time.</p><h4><strong>Food packaging could drive the next major wave of testing</strong></h4><p>PFAS concerns are not limited to the food itself.</p><p>The interview also examined <strong>food-contact materials</strong>, including takeout containers and other packaging from which PFAS may migrate into food. State-level restrictions in the United States are already beginning to affect the materials manufacturers can use.</p><p>For contract laboratories, this creates the possibility of another significant increase in demand for compliance and migration testing.</p><p>Taylor’s recommendation is straightforward: laboratories should follow proposed regulations before they become final.</p><p>Waiting until a new requirement takes effect can leave a laboratory simultaneously trying to acquire instrumentation, establish methods, train staff, and respond to customer demand. Monitoring regulatory agencies, scientific meetings, and industry discussions earlier gives laboratories time to prepare analytical capabilities before the testing requirement becomes urgent.</p><p>She expects PFAS restrictions in food packaging to continue developing first through a patchwork of individual state regulations. Once a sufficient number of states adopt restrictions, however, the economics may increasingly encourage suppliers to move toward PFAS-free materials regardless of whether every jurisdiction requires them.</p><p>A national standard could eventually follow that transition.</p><h4><strong>One sample, two regulatory environments</strong></h4><p>International food exporters face another complication: <strong>PFAS requirements are not necessarily the same between the United States and European Union</strong>.</p><p>Taylor noted that laboratories serving both markets may effectively need to operate under a dual-compliance model.</p><p>In the interview, she highlighted EU requirements targeting specific PFAS—including <strong>PFOS, PFOA, PFNA, and PFHxS</strong>—at very low concentrations in certain foods, while the U.S. approach has historically involved a different combination of monitoring programs, evolving compound lists, and import-related requirements.</p><p>For laboratories supporting international customers, this means more than simply selecting a different reporting limit.</p><p>Different markets may require different target lists, methods, documentation, auditing, and compliance procedures. Laboratories therefore need analytical platforms capable of ultratrace measurements while also maintaining the operational flexibility to support multiple regulatory frameworks.</p><p>That additional complexity also carries a practical cost: performing different protocols for different jurisdictions means more staff time, additional workflow management, and potentially greater instrument demand.</p><h4><strong>Total fluorine screening can help laboratories decide what to analyze next</strong></h4><p>Targeted LC-MS/MS methods are highly sensitive, but they only measure compounds included in the analytical panel.</p><p>This creates an important question: <strong>what about fluorinated substances that are present but are not included in the targeted method?</strong></p><p>The interview discussed <strong>EPA Method 1621</strong>, which uses a broader fluorine-based screening approach. Taylor described this type of measurement as useful for helping laboratories assess the gap between compounds captured by targeted LC-MS/MS methods and the broader amount of organofluorine present in a sample.</p><p>Such screening can also become part of a tiered workflow.</p><p>For applications such as food packaging, laboratories could initially use a broader fluorine measurement to determine whether a sample warrants further investigation. Positive or suspicious samples could then move to more selective and sensitive targeted analysis for identification and quantitation.</p><p>Non-targeted high-resolution mass spectrometry can add another layer by revealing compounds outside conventional target lists.</p><p>Together, these approaches create a funnel from broad screening toward increasingly detailed characterization rather than attempting the most comprehensive analysis on every sample from the outset.</p><h4><strong>PFAS analysis is becoming a data problem too</strong></h4><p>High-resolution mass spectrometry can generate enormous datasets containing signals from large numbers of known and unknown compounds.</p><p>As a result, Taylor believes the role of the analytical chemist is changing.</p><p>Strong laboratory skills remain essential, but scientists increasingly need <strong>data literacy</strong> as well. Understanding complex datasets, working with advanced software, and interacting effectively with artificial intelligence and machine-learning tools are becoming important parts of modern analytical work.</p><p>Taylor does not see this change primarily as replacing scientific expertise.</p><p>Instead, she compares it with earlier laboratory automation: repetitive or technically demanding tasks can increasingly be handled by software, allowing scientists to spend more time interpreting results and making decisions.</p><p>AI may also make sophisticated analytical information more accessible. A less experienced mass spectrometry user could potentially receive explanations of complex data through software capable of presenting results in natural language, reducing dependence on a single specialist being immediately available.</p><p>There is another potential advantage beyond speed.</p><p>Machine-learning systems can recognize patterns across large datasets that may be difficult or impossible for an individual scientist to detect manually. As analytical datasets become larger and multidimensional, that pattern-recognition capability could become increasingly valuable.</p><h4><strong>Finding PFAS is only the first half of the problem</strong></h4><p>For years, much of the PFAS discussion has focused on answering two questions:</p><ul><li><strong>Are PFAS present, and how much is there?</strong></li></ul><p>The next challenge is considerably harder: <strong>how do we actually remove or destroy them?</strong></p><p>Taylor described this as an expanding research field that will increasingly involve analytical laboratories.</p><p>The strength of the carbon-fluorine bond makes PFAS destruction difficult. Some treatment approaches seek to break this bond and achieve complete defluorination, but incomplete destruction can create another analytical problem.</p><p>For example, thermal treatment may transform longer-chain PFAS into shorter-chain fluorinated compounds rather than eliminating them completely. Those products may then enter another environmental compartment, including atmospheric emissions.</p><p>Consequently, laboratories studying PFAS destruction must do more than measure disappearance of the original compound. They also need to determine whether <strong>fluorinated transformation products or other byproducts remain</strong>.</p><p>Analytical chemistry therefore has two roles: monitoring PFAS contamination and demonstrating whether remediation technologies truly destroy the substances they are intended to remove.</p><h4><strong>The PFAS problem is expanding beyond water and food</strong></h4><p>The first two Labcompare documentaries focused primarily on <strong>water and food</strong>, two matrices already receiving substantial regulatory and public attention.</p><p>But Taylor does not expect the story to end there.</p><p>If Labcompare produces another PFAS documentary, she sees <strong>PFAS in air</strong> as a likely subject. Discussions with experts during earlier filming repeatedly raised atmospheric contamination, including both direct environmental occurrence and emissions potentially associated with treatment or destruction processes.</p><p>The progression illustrates how quickly the PFAS field is expanding.</p><p>Drinking water regulation accelerated analytical development. Food testing introduced more difficult matrices and new exposure pathways. Packaging is creating another compliance challenge. Environmental remediation raises questions about destruction products. Atmospheric monitoring could become another significant frontier.</p><p>For laboratories already working with chromatography and mass spectrometry, Taylor’s message is essentially to be prepared—even organizations that do not currently consider themselves “PFAS laboratories” may eventually become involved.</p><h4><strong>An analytical field that is still changing rapidly</strong></h4><p>Perhaps the strongest theme running through the interview is that PFAS analysis has no fixed endpoint yet.</p><p>Methods continue to evolve. Regulations change. Target compound lists expand. Instrument sensitivity improves. Laboratories discover new sources of background contamination, while researchers identify additional exposure pathways and investigate new destruction technologies.</p><p>Taylor compares the situation to earlier periods of rapid analytical development, such as the evolution of forensic DNA analysis: each technological advance reveals more information, which in turn creates new questions and new analytical requirements.</p><p>That makes preparation particularly important.</p><p>Laboratories cannot predict every future regulation, but they can follow proposed requirements, develop flexible workflows, invest in contamination control and data capabilities, and understand how their existing chromatography and mass spectrometry expertise could be applied to emerging PFAS challenges.</p><p>The analytical problem is no longer simply detecting a group of “forever chemicals.”</p><p>It is becoming a much larger question of <strong>how we identify them, quantify them, prevent contamination during analysis, understand their movement through food and the environment, comply with evolving regulations, and ultimately prove that they have actually been destroyed.</strong></p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

PFAS in Water, Food & Packaging: Michelle Taylor on the "Forever Chemicals" Documentary Series

Article

Scientists

Environment

Food

Water

Interview

Science and research

Video

Since its establishment in 2011, CEITEC has quickly developed into a cutting-edge infrastructure for research which performs highly alongside the best institutes in Europe. Among the main priorities of CEITEC are the promotion of a motivating and dynamic international scientific environment, the provision of state-of-the-art research infrastructure, and the policy of open communication and equal opportunities.

CEITEC

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Waters ACQUITY APC Columns

Waters ACQUITY Advanced Polymer Chromatography (APC) Columns with novel capabilities for chromatographic efficiency, speed and resolution that cannot be matched by conventional GPC methods.

<h4><strong>ACQUITY APC AQ BEH Column</strong></h4><p>ACQUITY APC AQ columns are designed to provide highly efficient size-based separations for water soluble polymer characterization. The sorbent uses patented Ethylene Bridge Hybrid (BEH) technology to provide a mechanical strong packed bed that resists shinking and swelling for easy mobile phase solvent switching. The expected molecular weight range for this column is approximatley 1, 000 - 30, 000 g/mole.</p><h4><strong>Specifications</strong></h4><ul><li><strong>Separation Mode:</strong> SEC/GPC</li><li><strong>Particle Substrate: </strong>Hybrid</li><li><strong>pH Range Min:</strong> 1 pH</li><li><strong>pH Range Max: </strong>8 pH</li><li><strong>Endcapped: </strong>No</li><li><strong>Silanol Activity: </strong>Medium</li><li><strong>Molecular Weight Range Min:</strong> 1000</li><li><strong>Molecular Weight Range Max:</strong> 30000</li><li><strong>Particle Shape: </strong>Spherical</li><li><strong>Particle Size:</strong> 1,7 µm &nbsp;/ 2.5 µm&nbsp;</li><li><strong>Endfitting Type: </strong>Parker-style</li><li><strong>System:</strong> APC</li><li><strong>Particle Technology:</strong> BEH</li><li><strong>Technique: </strong>SEC</li></ul><h4><strong>ACQUITY APC XT BEH Column</strong></h4><p>ACQUITY APC XT columns are optimized to provide highly efficient size-based separations for organic solvent soluble polymer characterization. The sorbent uses patented Ethylene Bridge Hybrid (BEH) technology to provide a mechanical strong packed bed that resists shinking and swelling for easy mobile phase solvent switching.The expected molecular weight range for this column is approximatley 1,000 - 30,000 g/mole.</p><h4><strong>Specifications</strong></h4><ul><li><strong>Separation Mode:</strong> SEC/GPC</li><li><strong>Particle Substrate: </strong>Hybrid</li><li><strong>pH Range Min:</strong> 1 pH</li><li><strong>pH Range Max: </strong>12 pH</li><li><strong>Endcapped: </strong>No</li><li><strong>Silanol Activity: </strong>Low</li><li><strong>Molecular Weight Range Min:</strong> 1000</li><li><strong>Molecular Weight Range Max:</strong> 30000</li><li><strong>Particle Shape: </strong>Spherical</li><li><strong>Particle Size:</strong> 1,7 µm &nbsp;/ 2.5 µm&nbsp;</li><li><strong>Endfitting Type: </strong>Parker-style</li><li><strong>System:</strong> APC</li><li><strong>Particle Technology:</strong> BEH</li><li><strong>Technique: </strong>GPC</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_APC_AQ_BEH_Column_0c0d837b73.jpeg" alt="Waters: ACQUITY APC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_APC_AQ_BEH_Column_0c0d837b73.jpeg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>ACQUITY APC Columns</strong></h3><h4><strong>Achieve superior speed and resolution for polymer characterization</strong></h4><p>Achieving superior performance and reliability with high resolution polymer characterization can be a challenge. <strong>ACQUITY Advanced Polymer Chromatography (APC) Columns</strong> are designed for polymer scientists like you, with novel capabilities for chromatographic efficiency, speed and resolution that cannot be matched by conventional GPC methods. With these columns, you can quantify and characterize polymer samples with confidence and accuracy while maximizing productivity.</p><p>The <strong>Ethylene Bridged Hybrid (BEH) particles</strong> used in ACQUITY APC Columns enable rapid solvent switching and the ability to use multiple conditions for the same bank of columns. Conventional polymeric stationary phases are dedicated to a single set of analytical conditions and solvent because the chromatographic bed is vulnerable to shrinking and swelling. By eliminating this undesirable behavior, ACQUITY APC Columns allow for maximum robustness and method development flexibility.</p><h4><strong>Overview</strong></h4><ul><li>Eliminate the shrinking and swelling that often occurs with polymeric stationary phases upon solvent switching with a stable chromatographic column bed&nbsp;</li><li>Achieve unmatched separation speed, resolution, and chromatographic efficiency from optimized pore volumes and particle size</li><li>Meet workflow requirements with 1.7 and 2.5 μm columns for organic and aqueous size-based separation</li><li>Achieve industry-leading column-to-column reproducibility with exceptionally reliable columns from a tightly controlled column manufacturing process</li><li><strong>Recommended Use: </strong>For rapid and accurate chromatographic characterization of synthetic polymer and macromolecular species.&nbsp;</li></ul><h4><strong>ACQUITY APC Column Featured Technologies</strong></h4><h5><strong>BEH Technology</strong></h5><ul><li>Rugged hybrid organic/inorganic base particle</li><li>Improved peak shape for bases- low silanol activity</li><li>Wider operating pH range for method development flexibility</li><li>Mechanical strength for maximum column efficiency at UHPLC operating pressures</li></ul><h4><strong>ACQUITY APC Column Characteristics</strong></h4><h5><strong>ACQUITY APC XT Columns</strong></h5><ul><li>Designed to provide high efficiency sized-based separations for organic soluble polymer characterization</li><li>Uses BEH particle technology</li><li>Stable column bed that resists shrinking and swelling for easy mobile-phase solvent switching</li><li>Features an extended temperature range up to 90 °C</li><li>Designed for organic solvent-based separations</li></ul><h5><strong>ACQUITY APC AQ Columns</strong></h5><ul><li>Designed to provide high efficiency sized-based separations for water soluble polymer characterization</li><li>Uses BEH particle technology</li><li>Robust separations up to 45 °C</li><li>Designed for aqueous-soluble polymer separations</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_APC_Columns_2268ceb5f5.jpeg" alt="Waters: ACQUITY APC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_APC_Columns_2268ceb5f5.jpeg 234w," sizes="100vw" width="1024" height="682"></p><h4><strong>Unprecedented capacity for rapid solvent switching</strong></h4><p>Use multiple conditions for the same column bank with rapid solvent switching using the rigid hybrid particles in ACQUITY APC Chromatography Columns. Conventional polymeric phases are often dedicated to single analysis conditions because the chromatographic bed is vulnerable to shrinking and swelling that can be potentially harmful to the column. By eliminating this constraint, ACQUITY APC Chromatography Columns provide more freedom for method development and deliver advanced polymer characterization for a diverse range of samples.</p><h4><strong>Reduce calibration time by a factor of 3-5x and increase resolution</strong></h4><p>With long conventional Gel Permeation Chromatography (GPC) run times of up to one hour or more, analyses of standards and samples can be quite time-consuming. In addition to long GPC run times, the large extra-column volume of conventional GPC systems can result in peak bandspreading, reducing the resolution and thus accuracy of the calibration points. Combined with the lower dispersion ACQUITY APC System, small particle ACQUITY APC Columns deliver less bandspreading and narrower, sharper peaks compared to the conventional GPC system. Additionally, robust sub-3-µm APC Column Technology supports higher flow rate and backpressures, thereby improving resolution.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_A_comparison_of_run_times_and_resolution_for_polystyrene_standards_Mp_100_K_10_K_and_1_K_on_a_conventional_GPC_system_and_the_ACQUITY_APC_System_37cfb15ed5.jpeg" alt="Waters: A comparison of run times and resolution for polystyrene standards (Mp: 100K, 10K, and 1K) on a conventional GPC system and the ACQUITY APC System. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_A_comparison_of_run_times_and_resolution_for_polystyrene_standards_Mp_100_K_10_K_and_1_K_on_a_conventional_GPC_system_and_the_ACQUITY_APC_System_37cfb15ed5.jpeg 234w," sizes="100vw" width="1024" height="682"></p><h4><strong>Ensure reproducible column performance</strong></h4><p>Reproducible column performance is one of the most critical factors for achieving long-term method reliability during polymer characterization. Each step of our column manufacturing process is monitored and tightly controlled to maintain unmatched product reproducibility. Every test, from raw material characterization to final batch analysis, is designed to ensure that ACQUITY APC Columns perform the same from column-to-column, batch-to-batch, and year-to-year.</p>

Waters SEC Columns

Get unmatched batch-to-batch reproducibility with the wide range of Waters Size-Exclusion Chromatography (SEC) Columns and standards for protein and peptide characterization.

<h4><strong>Size-based separations of peptides, proteins, nucleic acids, LNPs, and viral vectors</strong></h4><p>Get unmatched batch-to-batch reproducibility with the wide range of <strong>Waters Size-Exclusion Chromatography (SEC) Columns</strong> and standards for protein and peptide characterization.</p><p><strong>SEC</strong> is a powerful technique for aggregate and fragment analysis in the <strong>research, development</strong>, and <strong>manufacturing of biotherapeutic proteins</strong>, such as <strong>insulin</strong> and <strong>monoclonal antibodies</strong> (mAb). With SEC, also known as gel filtration, you can separate proteins based on their size (hydrodynamic radii versus absolute molecular weight) in solution, with larger sized species eluting before smaller proteins.</p><p>The <strong>pore size and volume</strong> of the SEC particles used in the packed column drives this <strong>isocratic based technique</strong>, so the column you choose matters. As the molecular weight of the proteins of interest increases, SEC columns with comparatively larger pore size particles are selected.</p><h4><strong>Overview</strong></h4><ul><li>Gain efficiency with this “platform based” method for <strong>proteins ranging from 10,000 to 650,000 Daltons</strong>, leveraging <strong>MaxPeak Premier Technology</strong> coupled with <strong>BEH-PEO Particle Technology&nbsp;</strong> &nbsp;&nbsp;</li><li><strong>Minimize non-desired secondary interactions </strong>for improved separation reproducibility and results with Ethylene Bridged Hybrid (BEH) SEC particles</li><li>Ensure reliable <strong>column-to-column and batch-to-batch performance</strong> with columns quality control tested with relevant peptide, protein, and mAb samples &nbsp;</li><li>Achieve reliable <strong>high throughput analysis on UPLC</strong> systems with our <strong>1.7 µm particle size</strong> offering and reproducible separations on any <strong>HPLC, UHPLC, or UPLC system</strong> to address different application needs with <strong>2.5 µm&nbsp;</strong></li><li><strong>Extend analytical column life</strong> and<strong> lower cost per analyses </strong>with SEC Guards&nbsp;</li><li>Confirm on-site SEC column and LC system performance with available BEH125, BEH200, BEH450, and mAb Size Variant Standards &nbsp;&nbsp;</li><li><strong>Manufactured in a cGMP, ISO 9001</strong> certified plant using stringent manufacturing protocols and ultra-pure reagents</li></ul><p><strong>Recommended Use: </strong>For size-based peptide, protein, and viral vector analyses.</p><h4><strong>System Dispersion Specifications for Column Recommendations</strong></h4><h5><strong>LC System Dispersions &lt;20 µL (UPLC)</strong></h5><ul><li>ACQUITY Premier Protein SEC 250 Å Guards and Columns</li><li>ACQUITY UPLC Protein BEH SEC 125 Å, 200 Å, and 450 Å Guards and Columns</li><li>1.7 µm guards and columns for low dispersion UPLC systems</li></ul><h5><strong>LC System Dispersions &gt;20 µL – ≤35 µL (UHPLC)</strong></h5><ul><li>XBridge Premier Protein SEC 250 Å Guards and Columns</li><li>XBridge Protein BEH SEC 125 Å, 200 Å, and 450 Å Guards and Columns</li><li>BioResolve mAb SEC Guards and Columns</li><li>2.5 µm guards and columns for UHPLC systems</li></ul><h5><strong>LC System Dispersions &gt;35 µL (HPLC)</strong></h5><ul><li>XBridge Protein BEH SEC 125 Å, 200 Å, and 450 Å Guards and Columns</li><li>XBridge Premier Protein SEC 250 Å Guards and Columns</li><li>BioResolve mAb SEC Guards and Columns</li><li>BioSuite SEC Guards and Columns</li><li>3.5 µm guards and columns for HPLC systems</li></ul><h4><strong>GTxResolve SEC Columns</strong></h4><h5><strong>GTxResolve Premier BEH SEC 450 Å Columns</strong></h5><ul><li>2.5 µm particle size columns for UHPLC and UPLC separations</li><li>Lower secondary interactions and lower noise suitable for platform methods</li><li>Low adsorption hardware necessary for analysis of biologics samples</li><li>450 Å pore size with up to 50% higher resolving power reducing need for sample consumption, resulting in faster analyses<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/nextgen/us/en/products/columns/GTxResolve-sec-columns.html"><strong>Explore GTxResolve SEC Columns</strong></a></li></ul></li></ul><h5><strong>GTxResolve Premier SEC 1000 Å Columns</strong></h5><ul><li>3 µm columns for UHPLC and UPLC separations</li><li>1000 Å columns with high efficiency for aggregate analysis of GTx drugs with diameter of 80-200 nm</li><li>Low secondary interaction &amp; improved compatibility with universally applied detector, such as MALS</li><li>Low adsorption hardware necessary for analysis of biologics samples<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/nextgen/us/en/products/columns/GTxResolve-sec-columns.html"><strong>Explore GTxResolve SEC Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_G_Tx_Resolve_SEC_Columns_1b8741a62b.jpg" alt="Waters: GTxResolve SEC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_G_Tx_Resolve_SEC_Columns_1b8741a62b.jpg 234w," sizes="100vw" width="1024" height="682"></p><h4><strong>Protein SEC Columns</strong></h4><h5><strong>ACQUITY Premier Protein SEC 250 Å Guards and Columns</strong></h5><ul><li>Sub-2-μm particle columns for UPLC separations</li><li>250 Å pore size</li><li>High throughput &amp; resolution SEC separations of biomolecules ranging from 10,000 to 650,000 Daltons on low dispersion UPLC Systems</li><li>Available SEC Guard Columns to extend analytical SEC Column life</li><li>Significantly reduce non-desired ionic and hydrophobic secondary interaction for desired SEC platform methods with MaxPeak Premier Technology and BEH-PEO SEC particle technology<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/nextgen/us/en/products/columns/acquity-premier-columns.html"><strong>Explore ACQUITY Premier Protein BEH SEC Guards and Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_Premier_Columns_featuring_Max_Peak_HPS_Technology_11a541350e.jpg" alt="Waters: ACQUITY Premier Columns featuring MaxPeak HPS Technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_Premier_Columns_featuring_Max_Peak_HPS_Technology_11a541350e.jpg 234w," sizes="100vw" width="1024" height="682"></p><h5><strong>XBridge Premier Protein SEC 250 Å Guards and Columns</strong></h5><ul><li>2.5 μm particle columns for HPLC and UHPLC separations</li><li>250 Å pore size</li><li>High resolution SEC separations of biomolecules ranging from 10,000 to 650,000 Daltons on any LC system</li><li>Available SEC Guard Columns to extend analytical SEC Column life</li><li>Significantly reduce non-desired ionic and hydrophobic secondary interaction for desired SEC platform methods with MaxPeak Premier Technology and BEH-PEO SEC particle technology</li><li>Specifically designed and QC tested with NIST-based, mAb Size Variant Standard to help ensure batch-to-batch consistency<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/global/Waters-XBridge-Premier-Columns/nav.htm"><strong>Explore XBridge Premier Protein BEH SEC Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_Premier_Columns_0230c90278.jpg" alt="Waters: XBridge Premier Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_Premier_Columns_0230c90278.jpg 193w," sizes="100vw" width="950" height="768"></p><h5><strong>BioResolve mAb SEC Guards and Columns</strong></h5><ul><li>2.5 μm particle columns for UPLC, UHPLC, and HPLC separations</li><li>200 Å pore size</li><li>Obtain reproducible, accurate quantitation of monoclonal antibodies (mAbs), associated high molecular weight aggregates (&gt;300,000 Da), and lower molecular weight fragments (e.g., &lt;100,000 Da)</li><li>Each packed column is tested with NIST-based, mAb size variant standard to help ensure batch-to-batch and column-to-column performance consistency<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/global/BioResolve-SEC-mAb-Consumables-%7C-SEC-Guards/nav.htm"><strong>Explore BioResolve mAb SEC Guards and Columns</strong></a></li></ul></li></ul><h5><strong>ACQUITY UPLC Protein BEH SEC Guards and Columns</strong></h5><ul><li>Sub-2-μm particle columns for UPLC separations (2.5 μm for 450 Å)</li><li>Pore size options include: 125 Å, 200 Å, and 450 Å</li><li>Available SEC Guard Columns to extend analytical SEC Column life</li><li>Specifically designed and QC tested with BEH125, BEH200, or BEH450 protein standards to help ensure batch-to-batch consistency</li><li>10x faster than traditional HPLC-based SEC<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/nextgen/us/en/products/columns/acquity-uplc-columns.html"><strong>Explore ACQUITY UPLC Protein BEH SEC Guards and Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Acquity_UPLC_kolony_1dc5c25d3e/Waters-Acquity-UPLC-kolony.jpg" alt="Waters: Acquity UPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Acquity_UPLC_kolony_1dc5c25d3e/thumbnail_Waters-Acquity-UPLC-kolony.jpg 245w," sizes="100vw" width="807" height="407"></p><h5><strong>XBridge Protein BEH SEC Columns</strong></h5><ul><li>2.5 μm particle columns for use on any UPLC, UHPLC, or HPLC system</li><li>Pore size options include: 125 Å, 200 Å, and 450 Å</li><li>Available SEC Guard Columns to extend analytical SEC Column life</li><li>Specifically designed and QC tested with BEH125, BEH200, or BEH450 protein standards to help ensure batch-to-batch consistency<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/global/XBridge-BEH-Columns-for-High-pH-Chromatography/nav.htm"><strong>Explore XBridge Protein BEH SEC Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_Columns_b0b55de501/Waters-XBridge-Columns.jpg" alt="Waters: XBridge Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_Columns_b0b55de501/thumbnail_Waters-XBridge-Columns.jpg 203w," sizes="100vw" width="900" height="691"></p><h4><strong>Peptide SEC Columns</strong></h4><h5><strong>BioSuite SEC Columns</strong></h5><ul><li>Silica-based diol SEC columns</li><li>Molecular weight exclusion limits are determined primarily by the selected pore size</li><li>Available in analytical and semi-preparative column sizes<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/global/BioSuite-Columns/nav.htm"><strong>Explore BioSuite SEC Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Bio_Suite_Columns_bf2bb8249f.jpg" alt="Waters: BioSuite Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Bio_Suite_Columns_bf2bb8249f.jpg 184w," sizes="100vw" width="307" height="260"></p><h5><strong>Protein-Pak SEC Columns</strong></h5><ul><li>Based on a 10 μm diol-bonded silica</li><li>Resolve proteins that differ in molecular weight by a factor of two</li><li>Distinguish proteins differing by as little as 15% in molecular weight<ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/global/Protein-Pak-HPLC-and-UPLC-Columns/nav.htm"><strong>Explore Protein-Pak SEC Columns</strong></a></li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Protein_Pak_Columns_be6a669326.jpg" alt="Waters: Protein-Pak Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Protein_Pak_Columns_be6a669326.jpg 245w," sizes="100vw" width="500" height="315"></p><h4><strong>Choose the right SEC Column for your lab</strong></h4><p>SEC is ideal for aggregate and fragment analysis in the research, development, and manufacturing of biotherapeutic peptides, proteins, and viral vectors, but choosing the right column for your analysis is important. Use the following criteria to choose a column that provides reliable, high quality SEC analyses of various biomolecules, including insulin, monoclonal antibodies, and adeno associated viruses (AAVs).</p><h5><strong>What to consider when choosing an SEC Column:</strong></h5><ul><li>What is the molecular weight of your sample?</li><li>What is the dispersion of your LC system?</li><li>Are you separating components &lt;2 fold difference in molecular weight (MW)?</li><li>Are you separating components &gt;2 fold difference in MW?</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Choose_the_right_Size_exclusion_chromatography_SEC_column_for_your_analysis_with_Waters_SEC_columns_selection_guide_b81f674f65.jpg" alt="Waters: Choose the right Size-exclusion chromatography (SEC) column for your analysis with Waters SEC columns selection guide." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Choose_the_right_Size_exclusion_chromatography_SEC_column_for_your_analysis_with_Waters_SEC_columns_selection_guide_b81f674f65.jpg 158w," sizes="100vw" width="1024" height="1013"></p><h4><strong>Addressing the complexity of SEC Separations</strong></h4><p>Experience the advancements in SEC-based technologies to address non-desired specific ionic and hydrophobic interactions for more reliable results with ACQUITY Premier Protein SEC and XBridge Premier Protein SEC Columns and Guards.</p><h5><strong>With MaxPeak Premier SEC Columns, you can:</strong></h5><ul><li>Reproducibly resolve injected sample components that are less than 2x different in size for challenging applications</li><li>Use a ‘platform SEC method’ to reduce method development time and cost</li><li>Ensure batch-to-batch and column-to-column performance</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Classes_of_therapeutic_proteins_separated_on_an_ACQUITY_Premier_Protein_SEC_Column_d3f74a8ea4.jpg" alt="Waters: Classes of therapeutic proteins separated on an ACQUITY Premier Protein SEC Column." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Classes_of_therapeutic_proteins_separated_on_an_ACQUITY_Premier_Protein_SEC_Column_d3f74a8ea4.jpg 234w," sizes="100vw" width="1024" height="682"></p>

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