Analysis of characteristic flavor compounds in coffee peels subjected to different thermal processing treatments based on GC–IMS and HS-SPME–GC–MS combined with chemical pattern recognition

Forced degradation analysis of recombinant adeno-associated virus serotype 8 based on analytical anion exchange chromatography coupled to orthogonal characterization

Combining elemental analysis and molecular techniques to characterize Ancient Egyptian mummification balms

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Watrex xTrovert XT Automatic DNA/RNA extractor

Watrex xTrovert XT is an automatic DNA/RNA extractor. It streamlines the extraction process, making it more efficient and reproducible.

Automatic extractor utilises a simple principle of two pipetting heads serving a 96-well plate.Thanks to easy replacement head options of pipetting with 300 μl tips or 1000 μl tips are available. Automatic optical sensor calibration enables the use of pipetting tips and 96-well plates from virtually any supplier. The device is equipped with two magnetic arms for protocols including paramagnetic SPR beads. It also allows for thermal incubation in the range of 25 to 85 °C. In addition to internal LED lighting, the station is equipped with an integrated UV-C sterilisation unit. Intuitive control from TFT touch panel user interface facilitates easy set-up. xTrovert™ XT is a fully programmable open system where user can create their own workflow scripts downloadable as text files from USB flas disk.<h3>APPLICATIONS</h3><ul><li>Sample prep for PCR testing</li><li>NGS library prep</li><li>Sanger DNA sequencing</li></ul><h3>CURRENT PROTOCOLS</h3><ul><li>DNA/RNA extraction</li><li>DNA purification</li><li>PCR clean-up/desalting</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Watrex_x_Trovert_XT_Automatic_DNA_RNA_extractor_Compatible_with_any_magnetic_bead_based_kit_23886eea52.jpg" alt="Watrex: Watrex xTrovert XT Automatic DNA/RNA extractor - Compatible with any magnetic bead based kit"><h3>TECHNICAL SPECIFICATION</h3><ul><li>Capacity: 16 or 32 samples/run</li><li>Volume range: 10 – 600 μl</li><li>Temperature: 25 – 85 °C</li><li>Sterilization: UV-C</li><li>Display: 3,5“ TFT touch display</li><li>Connectivity: USB 1.0/2.0</li><li>Power source: 24 V adaptor</li><li>Dimensions in cm: 65(w) x 65(h) x 34(d)</li><li>Weight: 25 kg</li></ul><h3>Ordering information:</h3><h5>PN/Component:</h5><ul><li>XTR000-001 xTrovert™ XT Universal robotic pipettor for DNA/RNA extraction &amp; handling</li><li>XTR000-003 Replaceable 8-channel 1000uL pipetting head for xTrovert™ XT</li><li>XTR000-004 Replaceable 8-channel 300uL pipetting head for xTrovert™ XT</li><li>XTR000-100 Deepwell plates (empty) for xTrovert™ XT</li><li>XTR000-220 DNA/RNA extraction kit, mgn. beads, 16-rxns per plate</li></ul>The entire system was designed and developed by Watrex Praha and is manufactured in the Czech Republic and promises a shift in the performance and efficiency of laboratory processes worldwide!You can also visit this product on the <a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/laboratory-automation/xtrovert-xt/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty">Watrex website</a>.

Identification of the substance – what is in your sample? (Bioanalytic)

We are able to identify metabolites and products of chemical reactions, contaminants of biological and chemical substances and preparations, and perform profiling of a series of samples to study the kinetics of transformations.

<h4>Substance Identification - Looking for answers to what's in your sample? - we can help you.</h4>Substance identification based on LC-MS/MS techniques can involve single compounds or mixtures of substances. In our analyses, we rely primarily on the knowledge and skills we have acquired after many years of work. For analysis, we use full laboratory facilities for sample preparation and high-resolution mass spectrometry (<a target="_blank" rel="noopener noreferrer" href="https://labexperts.com.pl/o-nas/">equipment</a>).We use different work algorithms depending on the material we are dealing with and the analytical task to be solved. We use DDA (IDA) or DIA (SWATH) methods or targeted approaches (e.g. MRMhr) if we know what to expect. Thus, we are able to identify metabolites and products of chemical reactions, contaminants of biological and chemical substances and preparations, and perform profiling of a series of samples to study the kinetics of transformations.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Identification_of_the_substance_what_is_in_your_sample_2_0f425a648b.jpg" alt="Bioanalytic: Identification of the substance – what is in your sample" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Identification_of_the_substance_what_is_in_your_sample_2_0f425a648b.jpg 245w," sizes="100vw" width="1881" height="1156"><h4>COST OF SERVICE</h4>We determine the cost of the service individually. The final cost is influenced by many factors related to the nature of the sample itself and the client's expectations. Thus, the price depends, among other things, on the complexity of the sample preparation procedure, the scope and number of analyses, etc.The service includes (depending on individual arrangements):<h5>Development of sample preparation procedure</h5><ul><li>Depending on the matrix and test substances.</li></ul><h5>Development of analytical methods</h5><ul><li>Application of high-resolution LC-MS/MS analyses using DDA (IDA) or DIA (SWATH) experiments and software to support the development of targeted screening methods.</li></ul><h5>Analysis of results includes:</h5><ul><li>finding and identifying the substances of interest, interpreting MS/MS spectra and proposing a structural formula.</li><li>Comparative analysis of sample series, e.g. PCA,</li><li>profiling of analytes in sample series</li></ul><h5>Reporting of results</h5><ul><li>Compile results into easy-to-read reports.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Identification_of_the_substance_what_is_in_your_sample_3_c1b8adc1d0.jpg" alt="Bioanalytic: Identification of the substance – what is in your sample" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Identification_of_the_substance_what_is_in_your_sample_3_c1b8adc1d0.jpg 245w," sizes="100vw" width="1919" height="1208">

Characteristics of monoclonal antibodies (Bioanalytic)

At LabExperts, proteomic analyses are performed on a ZenoTOF 7600 high-resolution LC-MS/MS system (SCIEX). For MS/MS analyses, we use Collision induced dissociation (CID) and Electron-Activated Dissociation (EAD) fragmentation.

Antibodies are molecules made up of 4 polypeptide chains: two heavy (H - high-chain) linked to each other by a specific type of chemical bond - a disulfide bridge, and two light (L - light-chain) linked by an identical bond to the heavy chains. Monoclonal antibodies (mABs) are used in various forms - as free molecules, in the form of a drug-antibody combination, and in the form of an immunoconjugate, a drug-antibody-biopolymer combination.Their main use is in highly specific targeted therapy for cancer, cardiovascular, autoimmune and nervous system diseases. They are also used in the treatment of osteoporosis, Alzheimer's disease or transplantation. The range of applications of monoclonal antibodies is systematically expanded. The introduction of a new antibody to the market requires complex product characterization, which involves numerous proteomic analyses, which are performed by LC-MS/MS techniques, among others.<h4>LC-MS/MS</h4>The characterization of monoclonal antibodies involves many aspects, including, but not limited to, a suite of analyses related to confirmation of the structure, chain sequence and degree of modification of the antibody. Post-translational modifications (PTMs) are chemical changes that occur after the translation process. These changes generally result in an increase in the immunogenicity of the drug. For this reason, it is essential to monitor modifications as critical quality attributes (CQAs) during both the production and storage of the drug. To ensure monitoring of CQA points, the primary structure of the protein is characterized based on peptide mapping (peptide mapping) analysis. The protein under study is subjected to proteolytic digestion using enzymes such as trypsin, chymotrypsin, Lys-C, Glu-C. As a result of various proteolytic enzymes, a wide range of peptides is obtained. Ultimately, coverage of the entire sequence of the protein under study is obtained.<figure class="image"><img style="aspect-ratio:1536/947;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Characteristics_of_monoclonal_antibodies_3_d94fa1623b.jpg" alt="Bioanalytic: Characteristics of monoclonal antibodies." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Characteristics_of_monoclonal_antibodies_3_d94fa1623b.jpg 245w," sizes="100vw" width="1536" height="947"></figure>At LabExperts, proteomic analyses are performed on a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?manufacturerItems=52&amp;search=ZenoTOF+7600">ZenoTOF 7600</a> high-resolution LC-MS/MS system (SCIEX). For MS/MS analyses, we use Collision induced dissociation (CID) and Electron-Activated Dissociation (EAD) fragmentation. This provides us with data for unambiguous identification of protein-building amino acids. We process the results using Sciex OS, Skyline and Spectronaut software.<blockquote>We perform:<ul><li>Whole protein analyses - Intact - to determine the exact mass and confirm the theoretical mass;</li><li>N-glycosylation profile determination (based on MS spectrum reconstruction):<ul><li>Glycosylated form;</li><li>Deglycosylated;</li><li>Glycosylated with reduced disulfide bridges;</li><li>Deglycosylated with reduced disulfide bridges.</li></ul></li><li>Peptide maps:<ul><li>Protein digestion with various proteases so that sequence coverage is close to 100%<ul><li>Analysis of PTMs:</li><li>Location of modifications</li></ul></li><li>[%] of modifications compared to the unmodified sequence</li></ul></li><li>TiPOS testing - (technical proof of similarity) - technical confirmation of the similarity of the protein synthesized by the customer (from several LOTs) with the target protein (provided as a template).</li></ul>In order to maintain the highest possible analytical standards, we perform monoclonal antibody analyses under the regime of an internally developed and implemented laboratory quality system based on GLP principles.See also our other proteomics services: <a target="_blank" rel="noopener noreferrer" href="https://labexperts.com.pl/service/identyfikacja-bialek/">Protein identification</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1294">Protein quantitative analysis</a></blockquote><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Characteristics_of_monoclonal_antibodies_2_cb93143fe6.jpg" alt="Bioanalytic: Bioanalytic Characteristics of monoclonal antibodies." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Characteristics_of_monoclonal_antibodies_2_cb93143fe6.jpg 245w," sizes="100vw" width="1536" height="908"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Characteristics_of_monoclonal_antibodies_1_0e8ab7cdfa.jpg" alt="Bioanalytic: Bioanalytic Characteristics of monoclonal antibodies." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Characteristics_of_monoclonal_antibodies_1_0e8ab7cdfa.jpg 245w," sizes="100vw" width="1536" height="858">

Determination of nitrosamine content (Bioanalytic)

Our offer includes comprehensive development and validation of methods for determination of nitrosamines in pharmaceutical products. We perform the work according to the criteria and needs of the client based on the EMA note.

Determination of nitrosamines is one of the pharmaceutical industry's top priorities. After the presence of N-nitrosodimethylamine (NDMA) was first reported in an active pharmaceutical ingredient (API), the U.S. Food and Drug Administration (FDA) and the European Medicines Agency (EMA) mandated the identification and control of all APIs and finished products in which there is a risk of nitrosamines.The content of nitrosamines in drug products can be controlled primarily by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis. More than that, a reliable analysis result in our offer consists of a well-chosen sample preparation procedure, a selective and sensitive analytical method, and the experience of the analyst, who selects the best method parameters at the optimization stage.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Determination_of_nitrosamine_content_Bioanalytic_2_ce4f440837.jpg" alt="Bioanalytic: Determination of nitrosamine content." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Determination_of_nitrosamine_content_Bioanalytic_2_ce4f440837.jpg 234w," sizes="100vw" width="1536" height="1024">We have gained experience working with leading pharmaceutical companies. As part of our ongoing work, we have developed analytical methods for small-molecule nitrosamines of 70-150 g/mol. These compounds are mainly formed during manufacturing processes and depend on the presence and type of excipients (e.g. NDBA - N-nitroso-dibutylamine). We have also developed methods for determining molecules with higher nitrosamine drug substance related impurities (NDSRIs). In our work, we focus on achieving the highest possible sensitivity, selectivity and precision. To this end, we individually select the parameters of the methods depending on the composition of the drug and the expected nitrosamines. Due to the above criteria and the required detection limits, we perform nitrosamine impurity determinations using LC-MS/MS kits based on <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?manufacturerItems=52&amp;search=QTRAP+5500%2B">QTRAP 5500+</a> and <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?manufacturerItems=52&amp;search=QTRAP+6500%2B">QTRAP 6500+</a> mass spectrometers (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?mainauthorItems=55">SCIEX</a>).<blockquote>Our offerOur offer includes comprehensive development and validation of methods for determination of nitrosamines in pharmaceutical products. We perform the work according to the criteria and needs of the client based on the EMA note.At the stage of sample preparation, we focus on:<ul><li>obtaining the highest possible recovery of nitrosamines (matrix purification);</li><li>limiting additional synthesis of impurities (selection of appropriate equipment and reagents);</li><li>ensuring the stability of the sample prepared for LC-MS/MS analysis.</li></ul>During the optimization of the LC-MS/MS method, we solve such problems as:<ul><li>selection of an appropriate internal standard;</li><li>separation of the impurity from the API;</li><li>obtaining the expected sensitivity of the analytical method.</li></ul>Quantitative analysis of nitrosamines requires sensitivity, precision and accuracy, which we provide to our customers.</blockquote><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Determination_of_nitrosamine_content_Bioanalytic_1_d629e00e62.jpg" alt="Bioanalytic: Determination of nitrosamine content." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Determination_of_nitrosamine_content_Bioanalytic_1_d629e00e62.jpg 103w," sizes="100vw" width="1130" height="1710">

Watrex application and R&D laboratory

Watrex application and R&D laboratory is providing a range of analyses.

Watrex application and R&amp;D laboratory is providing a range of analyses with special focus in the following fields:<ul><li>Chemical analysis for construction industry</li><li>Environmental analysis</li><li>Sample preparation including online solid-phase extraction protocols</li><li>Development of methods for pharmaceutical analysis</li><li>Characterization of synthetic polymers by GPC</li><li>Column technology and methods for chiral separation</li><li>Parameters evaluation and testing in ion chromatography applications</li></ul><h5>Our ready-to-go tests include:</h5><ul><li>Analysis of carbohydrates</li><li>Characterization of polymers</li><li>Analysis of organic acids</li><li>Construction analysis</li><li>Analysis of ions, pH and water hardness</li></ul>

ECOM PREPBOX A3L8E Chromatography Separation System

PREPBOX is a compact alternative to modular preparative systems. It is assembled according to the customer's wishes.

<div style="display:block;" aria-labelledby="tab_item-0">Complete preparative modular system with one basic gradient pump and optionally secondary pump for repetitive sampling or with injection valve. Also switching valve for&nbsp;<abbr title="Countercurrent Chromatography">CCC</abbr> applications. PDA four channel detector up to 800&nbsp;nm.&nbsp;It is assembled according to the customer's wishes. It is used for high-capacity systems or with centrifugal chromatography systems like in pharmaceutical purification of cannabis extracts (<abbr title="Cannabidiol">CBD</abbr>, <abbr title="Cannabinol">CBN</abbr>, <abbr title="Cannabigerol">CBG</abbr>, <abbr title="Cannabichromene">CBC</abbr>, <abbr title="Tetrahydrocannabinol">THC</abbr>...).ECOMAC SW installed at internal PC controls the system. Fractions are collected by means of&nbsp;10 position valve&nbsp; It is necessary to purchase an operating system for the PREPBOX&nbsp;- in most cases we recommend Windows OS&nbsp;(P/N:&nbsp;ABB90000) or Linux&nbsp;(on request). The system also supports the Clarity software. It is also possible to purchase a wireless mouse and keyboard set&nbsp;(P/N:&nbsp;EPC-0028) and a computer monitor&nbsp;(P/N:&nbsp;EPC-0027).<h5>TOY18DAD 800L Detector</h5>It is UV-VIS diode array detector, which allow measuring absorbance of four wavelengths in range of 200-800&nbsp;nm simultaneously in one cell just as scanning of whole spectra.<h5>ECP201L Preparative HPLC Pump</h5>Preparative pump suited for preparative liquid chromatography with very high pressure up to 15&nbsp;MPa&nbsp;at flow rate of 1000&nbsp;ml/min. Advantage of this pump is a very stable run even at low flow rates.<h5>ECP2300 Preparative Gradient Pump</h5>Preparative HPLC pump suited for&nbsp;preparative&nbsp;applications in liquid chromatography with a pressure up to 15 MPa at flow rate of 300&nbsp;ml/min.&nbsp;New sophisticated unit allows achieving a precise&nbsp;low-pressure gradient, i.e. gradual changes of solvent composition by mixing&nbsp;up to four liquids&nbsp;at the pump entry.<h3>Parameters</h3><ul><li>Interface: 5x USB, HDMI, 2X RS232, LAN, VGA</li><li>Power supply: 100 - 240 V AC</li><li>Power input: 490 W</li><li>Dimensions W x H x D: 405x490x525 mm (15.94x19.29x20.67 in)</li><li>Weight: 55 kg</li></ul></div>

<div style="display:block;" aria-labelledby="tab_item-0">Complete preparative modular system with one basic gradient pump and optionally secondary pump for repetitive sampling or with injection valve. Also switching valve for&nbsp;<abbr title="Countercurrent Chromatography">CCC</abbr> applications. PDA four channel detector up to 800&nbsp;nm.&nbsp;It is assembled according to the customer's wishes. It is used for high-capacity systems or with centrifugal chromatography systems like in pharmaceutical purification of cannabis extracts (<abbr title="Cannabidiol">CBD</abbr>, <abbr title="Cannabinol">CBN</abbr>, <abbr title="Cannabigerol">CBG</abbr>, <abbr title="Cannabichromene">CBC</abbr>, <abbr title="Tetrahydrocannabinol">THC</abbr>...).ECOMAC SW installed at internal PC controls the system. Fractions are collected by means of&nbsp;10 position valve&nbsp; It is necessary to purchase an operating system for the PREPBOX&nbsp;- in most cases we recommend Windows OS&nbsp;(P/N:&nbsp;ABB90000) or Linux&nbsp;(on request). The system also supports the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/999">Clarity software</a>. It is also possible to purchase a wireless mouse and keyboard set&nbsp;(P/N:&nbsp;EPC-0028) and a computer monitor&nbsp;(P/N:&nbsp;EPC-0027).<h5>TOY18DAD 800L Detector</h5>It is UV-VIS diode array detector, which allow measuring absorbance of four wavelengths in range of 200-800&nbsp;nm simultaneously in one cell just as scanning of whole spectra.<h5>ECP2300 Preparative Gradient Pump</h5>Two peparative HPLC pump suited for&nbsp;preparative&nbsp;applications in liquid chromatography with a pressure up to 15 MPa at flow rate of 300&nbsp;ml/min.&nbsp;New sophisticated unit allows achieving a precise&nbsp;low-pressure gradient, i.e. gradual changes of solvent composition by mixing&nbsp;up to four liquids&nbsp;at the pump entry.<h4>Complete preparative modular system with:</h4><ul><li>one basic gradient pump</li><li>for sampling secondary pump or injection valve</li><li>optionally switching valve for CCC</li><li>PDA detector up to 800 nm</li><li>10 positions valve for fractions collecting</li><li>PC board with ECOMAC SW</li></ul><h3>Parameters</h3><ul><li>Interface: 5x USB, HDMI, 2X RS232, LAN, VGA</li><li>Power supply: 100 - 240 V AC</li><li>Power input: 4900 W</li><li>Dimensions W x H x D: 405x490x525 mm (15.94x19.29x20.67 in)</li><li>Weight: 50 kg</li></ul></div>

Wiley KnowItAll GC-MS Drug Classification Model Package

The KnowItAll GC-MS Drug Classification Model Package categorizes spectra by drug class—even without a match. Covers opioids, steroids, fentanyls, with 99% accuracy for reliable forensics.

<h4>Efficient Drug Classification with KnowItAll GC-MS Models</h4>In drug analysis, rapid identification is essential—especially when new substances outpace reference spectra. The KnowItAll GC-MS Drug Classification Model Package helps labs classify unknown spectra, even without exact spectral matches.With 99% accuracy, these models cover key drug classes like opioids, steroids, fentanyls, and more. Ideal for forensics, toxicology, and law enforcement, the package enhances your ability to:<ul><li>Classify spectra not found in libraries</li><li>Validate spectral search results</li><li>Make fast, first-pass identifications</li><li>Run batch or automated classifications using the KnowItAll Automate SDK</li></ul><h5>Included Drug Models:</h5><ul><li>Natural &amp; synthetic opioids</li><li>Fentanyls</li><li>Piperazines</li><li>Benzodiazepines</li><li>Steroids</li><li>Ketamine, cocaine, cathinones &amp; related compounds</li></ul>Requirements: KnowItAll software + AVX-compatible system. Learn more: <a target="_blank" rel="noopener noreferrer" href="https://sciencesolutions.wiley.com">sciencesolutions.wiley.com</a>

Wiley LIPIDS Mass Spectral Database

The Lipids Mass Spectral Database includes 430 GC spectra from pure standards across 11 lipid classes. It supports peak assignment in complex mixtures and is valuable for research in food analysis, clinical, and medical applications.

<h4>Validated Lipid Identification Made Simple</h4>The Lipids Mass Spectral Database features 430 GC-MS spectra of pure lipid standards across 11 lipid classes, supporting accurate peak identification in complex mixtures. It's an essential tool in food, clinical, and medical research.With over 1,400 Linear Retention Index (LRI) values determined on three different GC columns and using multiple reference mixtures, the library ensures reliable retention data. Each entry includes detailed metadata such as CAS numbers, formulas, and suppliers.Highlights:<ul><li>430 spectra, 428 unique compounds</li><li>11 lipid classes including FAMEs, sterols, and wax esters</li><li>Experimentally validated LRI on SLB-5ms, Equity-1, Supelcowax-10</li><li>Rich compound metadata for confident identification</li></ul>Explore compound classes at <a target="_blank" rel="noopener noreferrer" href="https://www.compoundsearch.com">www.compoundsearch.com</a>

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Combining elemental analysis and molecular techniques to characterize Ancient Egyptian mummification balms

Forced degradation analysis of recombinant adeno-associated virus serotype 8 based on analytical anion exchange chromatography coupled to orthogonal characterization

Analysis of characteristic flavor compounds in coffee peels subjected to different thermal processing treatments based on GC–IMS and HS-SPME–GC–MS combined with chemical pattern recognition