ISC 2026 - Day 2

<h4>What if you could compress 179 days of grueling laboratory workflow into just 14 hours?</h4>In this episode of ‪ChromatographyTalk‬, ‪Organomation‬ General Manager David Oliva sits down with analytical chemist Laura Penabad to discuss her groundbreaking new research in biocatalysis and high-throughput screening. Evolving engineered enzymes typically requires checking thousands of variants—a massive bottleneck when traditional Liquid Chromatography-Mass Spectrometry (LC-MS) runs take minutes per sample. Laura reveals how her team built a modular screening platform that shatters this limitation, running samples 128 times faster than standard methods without sacrificing analytical accuracy.Laura breaks down the "orchestra" of technologies making this possible, including coupling nanoliter droplet microfluidics with Cyclic Ion Mobility-Mass Spectrometry (cIM-MS) to separate complex chemical isomers in milliseconds. She also shares the chaotic but triumphant behind-the-scenes stories of her PhD journey—from presenting a "comically bad" early poster at ASMS to recruiting an undergraduate mechanical engineer to build custom automation software from scratch.If you are an early-career researcher, a mass spec enthusiast, or curious about the future of synthetic biology and machine learning in chemistry, this episode is packed with invaluable intuition and advice.<h3>Video Transcription</h3><h4>From Months to Hours: Droplet Ion Mobility–MS for Biocatalysis Screening</h4>High-throughput screening is central to directed enzyme evolution, but conventional analytical workflows can create a difficult compromise: extremely fast screens often provide limited structural information, while techniques such as LC-MS offer much richer chemical insight at the cost of throughput. In a recent interview, analytical chemist Laura described how her work with droplet-based cyclic ion mobility mass spectrometry is helping bridge that gap—providing rapid analysis while retaining the ability to distinguish structurally similar products.Her research grew from an initially modest proof of concept into a screening platform capable of analyzing a 96-well plate in roughly ten minutes. The resulting workflow was approximately 128 times faster than a conventional three-minute LC-MS analysis, opening new possibilities for directed evolution campaigns in which thousands of enzyme variants need to be evaluated.<h4>Directed evolution needs better analytical screening</h4>Biocatalysis takes advantage of enzymes as highly selective catalysts capable of performing reactions under relatively mild conditions. Directed evolution extends those capabilities by deliberately modifying enzyme sequences, expressing large numbers of variants, screening their activity, and using the best-performing mutants as starting points for subsequent rounds of evolution.The process can be repeated multiple times, progressively steering an enzyme toward improved activity, altered substrate specificity, or even chemistry that does not occur naturally.But the effectiveness of directed evolution depends heavily on the screening step. The more variants that can be analyzed—and the more useful chemical information that can be collected for each one—the more effectively researchers can explore the enormous sequence space available to an enzyme.For Laura, this created a clear analytical challenge: develop a screening platform that was substantially faster than conventional chromatographic analysis without sacrificing the structural discrimination needed for chemically similar products.<h4>From an imperfect first experiment to a working platform</h4>The project did not begin with a polished high-throughput workflow.Laura recalled looking back at her 2023 ASMS poster, where the early system was still working with standards rather than true isomeric samples. Droplet introduction was slow—around six seconds per droplet—and the signals were far from the ideal reproducible, square, flat-topped profiles expected from replicate droplets.Nevertheless, simply producing a droplet signal represented an important first milestone.The decisive change came when the project stopped being treated merely as a demonstration that droplet ion mobility–MS could work and instead became a deliberate effort to build a robust screening platform.Laura approached the problem in modules. First came reliable droplet introduction. Then the ion mobility separation method. Quantification became another independent component. Matrix effects were introduced later, gradually making the system resemble the conditions expected with real screening samples.Each layer was tested before another was added.This stepwise strategy became one of the central principles of the project: rather than attempting to optimize the entire system simultaneously, individual components were developed, challenged, and then assembled into an increasingly realistic workflow.<h4>Finding the middle ground between speed and structural information</h4>One of the biggest analytical problems in high-throughput screening is distinguishing products with the same nominal or exact mass.Constitutional isomers may contain the same atoms and therefore generate the same mass-to-charge ratio, while differing only in how functional groups are arranged within the molecular structure. Very rapid optical or mass-based screens may therefore struggle to distinguish them.Traditional LC-MS can solve many of these problems through chromatographic separation, but a several-minute analysis rapidly becomes impractical when thousands of enzyme variants must be screened.Ion mobility offered Laura’s team another option.Rather than separating compounds on a chromatographic timescale, ion mobility can differentiate ions according to their gas-phase behavior on a much shorter timescale. In the workflow described in the interview, this created a useful compromise: not the kilohertz throughput possible with some simple screening technologies, but dramatically faster structural differentiation than conventional LC-MS.The goal was therefore not simply to achieve the fastest possible screen, but to find a middle-throughput analytical regime with enough resolving power to answer chemically meaningful questions.<h4>A 96-well plate in about ten minutes</h4>That strategy eventually produced one of the most striking results of the project.The droplet cyclic ion mobility–MS platform processed a 96-well plate in roughly ten minutes, while maintaining quantitative results comparable with the conventional LC-MS workflow used for comparison. According to the interview, this represented an approximately 128-fold increase in analysis speed relative to a three-minute LC-MS run.Laura described the successful plate experiment less as a surprise than as confirmation that the modular development strategy had worked.Before reaching that point, the workflow had already been challenged through a sequence of increasingly demanding experiments: droplet formation, ion mobility separation, calibration, matrix-containing calibration, and cross-testing analytes at different concentrations. By the time the complete plate was analyzed, each individual part of the analytical system had already been tested under progressively more realistic conditions.<h4>What does that speed mean for directed evolution?</h4>The impact becomes even clearer when the workflow is considered at the scale of an entire directed-evolution campaign.Laura’s manuscript estimated that a five-round campaign involving 5,000 variants would require approximately 179 days using the conventional LC-MS workflow but only about 14 hours using the new platform.Compressing months of analytical work into less than a day has consequences beyond laboratory productivity.It allows researchers to screen more enzyme variants and therefore sample a larger portion of sequence space. But the resulting datasets may also become increasingly valuable for computational approaches.Rather than retaining information only about the best-performing enzyme variant, researchers can preserve data describing variants that performed poorly as well as those that succeeded. When genotype and phenotype information remain linked through the well-plate workflow, these larger datasets can potentially provide richer input for machine-learning models designed to guide subsequent rounds of enzyme evolution.Faster analysis therefore does more than accelerate an existing experiment—it can change the scale and design of the experiment itself.<h4>Balancing ion mobility resolution and signal</h4>Increasing cyclic ion mobility separation time can improve resolution, but it also introduces an important trade-off: additional passes can reduce signal.Laura optimized this balance experimentally by continuously infusing mixtures containing both isomers and progressively adjusting the ion mobility conditions. After tuning parameters such as wave height and velocity, increasing the separation to three passes provided baseline resolution at the high analyte concentrations used during method development.<blockquote>Concentration, however, also mattered.</blockquote>At higher ion populations, space-charge effects could broaden peaks and reduce the baseline separation. This meant that the optimal number of passes could not be selected independently of the concentration range required by the final assay.Her broader advice for optimization is therefore application-driven: decide first what the analytical method actually needs to accomplish.More speed will almost always require sacrificing something—resolution, signal intensity, quantitative precision, or another performance parameter. The key is to identify which compromise the application can tolerate and then optimize the system around that requirement.<h4>Solving droplet carryover with a practical compromise</h4>Droplet-based workflows present their own engineering challenges, including carryover between neighboring samples.In Laura’s system, analytes showed little tendency to partition into the fluorinated oil separating aqueous droplets within the Teflon tubing. The more troublesome carryover occurred closer to electrospray ionization, where a small amount of sample could remain associated with the interface and appear when the next droplet arrived.Rather than adding excessive engineering complexity to an already elaborate platform, the researchers adopted a pragmatic solution: a sacrificial wash droplet between analytical samples.It is an example of a recurring theme throughout the project. The objective was not to construct the theoretically most sophisticated droplet system, but to create a screening workflow that remained sufficiently robust, reproducible, and practical for large numbers of samples.<h4>High-throughput analysis also requires high-throughput data processing</h4>Accelerating the mass spectrometry experiment created another bottleneck: the data.The instrument produced a continuous intensity-versus-time trace rather than a neatly organized well-plate dataset. With small experiments, Laura could manually identify individual droplets and process the results in spreadsheets. That approach quickly became unrealistic as sample numbers increased.The solution emerged through collaboration with an undergraduate mentee, Aiden, who helped develop a dedicated software tool for processing the continuous traces.Laura defined how the software should behave and how the analytical information should be organized, while Aiden developed the computational implementation. The workflow itself was subsequently adjusted to make automated data processing more reliable—for example, by introducing marker columns that allowed the software to recognize the end of each plate row.The resulting tool can accept the acquired traces together with information about plate dimensions and replicates and automatically return parameters including peak identity, duration, and the corresponding well position. A later version also generated an automatically populated heat map.The experience illustrates how improving analytical throughput often requires simultaneous development of instrumentation, experimental design, and informatics. Making the measurement faster is useful only if the resulting data can be processed at a comparable speed.<h4>Mentoring became part of the scientific process</h4>The software project also reflects another theme Laura emphasized throughout the interview: the value of mentoring.Several younger researchers contributed directly to the development of the final platform. Laura described mentees not simply as additional hands in the laboratory but as scientists capable of proposing their own hypotheses and experimental ideas.Working with undergraduate researchers also influenced the way she organized experiments.Because their available laboratory time was limited, each task needed to have a clear purpose. That encouraged a more disciplined approach to experimental planning: instead of performing experiments simply because they could be done, the team had to ask which experiment would most efficiently answer the next important question.In that sense, mentoring became another mechanism for improving the research itself.<h4>The next step: continuous, automated screening</h4>Despite the dramatic increase in analytical speed, the current workflow still includes manual operations such as loading tubing and connecting the sample stream to the mass spectrometer.The next engineering challenge is therefore automation.Laura described ongoing work using the Venturi effect to pull samples directly from wells into the droplet system. Building on previous work in the laboratory with 96-well plates, she has already tested this approach with as many as 2,000 samples.The long-term direction is clear: continuous and robust droplet generation, automated handling of thousands of samples, rapid ion mobility separation, mass spectrometric detection, and automated reconstruction of the resulting data into plate-based outputs.Combining those elements could move the system closer to the type of large-scale screening required for increasingly ambitious biocatalysis campaigns.<h4>Learning mass spectrometry one problem at a time</h4>Laura also reflected on the broader experience of developing the platform during her PhD.Ion mobility and mass spectrometry initially appeared intimidating, particularly because they combine concepts from analytical chemistry, physical chemistry, separations, instrumentation, and electrical engineering. What eventually changed was not simply learning more facts, but developing the ability to move repeatedly between theory and experiment.An unexpected result sends the researcher back to the underlying theory. A better understanding suggests a modified experiment. That experiment creates another observation, gradually building intuition about how the system behaves.Her advice to early-career scientists is therefore to accept that this process takes time.Advanced analytical techniques may initially appear overwhelming, but understanding develops incrementally through repeated interaction between theory, experimentation, failure, and refinement. Equally important, she emphasized the role of the supportive ion mobility–mass spectrometry community in making a technically demanding field more approachable for young researchers.From the first inconsistent droplets to a platform capable of processing thousands of samples, that iterative mindset ultimately shaped the entire project.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Droplet Cyclic Ion Mobility Mass Spec: Compressing Months of Enzyme Screening Into Just 14 Hours

ISC 2026 - Day 3

Article

Events

Video

Scientists

Interview

The International Symposium on Chromatography (ISC) represents the oldest conference series focussing on separation science. ISC symposia have been organised since 1956 in each even year. ISC is internationally recognised as one of the premier meeting series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.

International Symposium on Chromatography - (ISC)

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Thermo Scientific Dionex IonPac AS19 IC Columns

Measure trace bromate and other oxyhalides in drinking water, ground water, wastewater, and other diverse sample matrices with Thermo Scientific Dionex IonPac AS19 Columns.

Measure trace bromate and other oxyhalides in drinking water, ground water, wastewater, and other diverse sample matrices with Thermo Scientific™ Dionex™ IonPac™ AS19 Capillary, Analytical &amp; Guard Columns. The column is specifically designed and recommended for determination of trace bromate, a toxic byproduct of ozone disinfection. The column's high capacity and selectivity facilitate the determination of bromate in drinking water at the low-μg/L level. The Dionex IonPac AS19 columns meet the performance requirements of U.S. EPA Methods 300.0 and 300.1.<h5>Reduce Operating Costs With our New Capillary Column</h5>The Dionex IonPac AS19 Capillary column is packed with the same material as the equivalent standard bore version (providing the same performance as a 4 mm column), but requires only 1/100th the eluent flow rate. The capillary format provides the advantage of significantly reduced eluent consumption, which helps reduce operating costs.<h5>Superior Chromatographic Performance</h5><ul><li>High capacity: 240μeq per column (4x250mm)</li><li>Operates at ambient or elevated temperatures with column selectivity optimized for 30°C</li><li>Compatible with organic solvents</li><li>Low background signal and enhanced analyte sensitivity when using the Thermo Scientific™</li><li>Dionex™ Anion Self-Regenerating Suppressor (ASRS™ 300) and a Reagent-Free™ (RFIC™) system</li><li>Analyze a Wide Range of Oxyhalides and Inorganic Anions</li><li>Oxyhalides and common inorganic anions including fluoride, chlorite, bromate, chloride, nitrite, bromide, chlorate, nitrate, phosphate, and sulfate in drinking water, groundwater, wastewater, and other diverse sample matrices.</li><li>Analyzes most drinking water without the use of sample pretreatment or preconcentration.</li><li>Ideal when used with a Reagent-Free™ Ion Chromatography (RFIC)™ system for automatic eluent generation</li></ul><h5>Designed for Detection of Trace Bromate</h5>The key application for this column is the determination of trace bromate in drinking water matrices using a potassium hydroxide gradient with suppressed conductivity detection. The column's selectivity ensures that bromate can be quantified at low μg/L concentrations using suppressed conductivity detection even in the presence of very high concentrations of chloride, sulfate, and carbonate.<h4>Specifications</h4><ul><li>Anion Exchange Capacity: 240 μeq</li><li>Crosslinking: 55% DVB</li><li>Column Capacity: 240 μeq</li><li>Column Chemistry: Hydroxide</li><li>For Use With (Application): Analysis of Trace Bromate in Drinking Water</li><li>Ion Exchange Group: Alkanol Quaternary Ammonium Ion</li><li>Max. Pressure: 3000 psi (207 bar)</li><li>Solvent Compatibility: pH 0 to 14, 0 to 100% HPLC Solvents</li><li>Stationary phase: IonPac AS19</li><li>Format: Standard Bore</li><li>Packing Material: Polymeric</li></ul>

Thermo Scientific Dionex IonPac AS20 IC Columns

Simplify trace analysis of perchlorate in drinking water, surface water, and groundwater matrices using the Thermo Scientific Dionex IonPac AS20 Columns.

Simplify trace analysis of perchlorate in drinking water, surface water, and groundwater matrices using the Thermo Scientific™ Dionex™ IonPac™ AS20 Capillary, Analytical &amp; Guard Columns. The capacity and selectivity of this column ensures that perchlorate can be quantified at low μg/L concentrations using suppressed conductivity detection, even in the presence of very high concentrations of chloride, carbonate, and sulfate. This column is specified in U.S. EPA Method 314.1 for the determination of trace perchlorate in drinking water (Confirmatory Method) after sample preconcentration.<h5>Reduce Operating Costs With our New Capillary Column</h5>The Dionex IonPac AS20 Capillary column is packed with the same material as the equivalent standard bore version (providing the same performance as a 4mm column) but requires only 1% of the eluent flow rate. The capillary format provides the advantage of significantly reduced eluent consumption, which helps reduce operating costs.<h5>Superior Chromatographic Performance</h5><ul><li>Recommended hydroxide-selective, anion-exchange column for trace perchlorate in environmental water samples</li><li>High Capacity: 310μeq per column. (4 x 250mm)</li><li>Increased mass sensitivity, reduced eluent consumption, and reduced operating costs</li><li>Operates at ambient or elevated temperature; column selectivity is optimized for 35°C operating temperature</li><li>Compatible with organic solvents</li></ul><h5>For Best Results</h5><ul><li>Use this column with a Reagent-Free™ IC (RFIC™) system and an eluent generator for optimum detection limits.</li><li>Use the Thermo Scientific™ Dionex™ Anion Electrolytically Regenerated Suppressor (AERS™ 500) or the Thermo Scientific™ Dionex™ Anion Capillary Electrolytic Suppressor (ACES™ 300) with the Dionex IonPac AS20 column for eluent suppression and enhanced analyte sensitivity.</li><li>Use the Thermo Scientific™ Cryptand C1 Concentrator column for sample preconcentration for analysis of trace perchlorate with the analytical column.</li></ul><h4>Specifications</h4><ul><li>Anion Exchange Capacity: 310 μeq</li><li>Crosslinking: 55% DVB</li><li>Column Capacity: 310 μeq</li><li>Column Chemistry: Hydroxide</li><li>For Use With (Application): Anion Exchange</li><li>Ion Exchange Group: Alkanol Quaternary Ammonium Ion</li><li>Max. Pressure: 4000 psi (275 bar)</li><li>Solvent Compatibility: pH 0 to 14, 0 to 100% HPLC Solvents</li><li>Stationary phase: IonPac AS20</li><li>Format: Standard Bore</li><li>Packing Material: Polymeric</li></ul>

Thermo Scientific Dionex IonPac AS22 IC Columns

Thermo Scientific Dionex IonPac AS22 IC Columns provide fast analysis of drinking water, ground water and wastewater.

Designed specifically for compliance monitoring of inorganic anions in accordance with U.S. EPA Methods 300.0 (A), the Thermo Scientific™ Dionex™ IonPac™ AS22 IC Columns provide fast analysis of drinking water, ground water and wastewater. With the Dionex IonPac AS22 column, using a simple isocratic carbonate/bicarbonate eluent, common inorganic anions can be quickly separated in a variety of sample matrices including drinking water, wastewater, process streams, and scrubber solutions.<h5>Analysis of Common Inorganic Anions and Low Molecular Weight Organic Acids in Diverse Matrices</h5><ul><li>Compliance monitoring of common anions in drinking water as specified in U.S. EPA Method 300.0 (A)</li><li>Fast isocratic separation of common inorganic anions in simple matrices in 8 min.</li><li>Isocratic separation of common inorganic anions in complex matrices in 12 min.</li><li>Excellent retention of fluoride from the water dip and resolution of fluoride, acetate, and formate</li><li>Excellent resolution of the carbonate peak away from peaks for common inorganic anions</li><li>Ideal alternative, using carbonate/bicarbonate eluents, for IonPac AS4A-SC, AS12A, AS14 and AS14A inorganic anion applications.</li><li>Solvent compatibility permits easy column cleanup after the analysis of samples with hydrophobic components.</li></ul><h5>Multiple Formats Available for Application Flexibility</h5><ul><li>4x250mm, 2x250mm analytical columns</li><li>4x50mm, 2x50mm guard columns</li><li>Longer 250mm columns facilitate higher resolution separations of more complex sample matrices</li><li>Smaller i.d. columns provide higher mass sensitivity, eluent and reagent conservation and lower operating costs</li></ul>Note: For simple, accurate eluent preparation, use the IonPac AS22 Eluent Concentrate.<h4>Specifications</h4><ul><li>Anion Exchange Capacity: 210 μeq</li><li>Crosslinking: 55% DVB</li><li>Column Capacity: 210 μeq</li><li>Column Chemistry: Carbonate/Bicarbonate</li><li>For Use With (Application): Anion Exchange</li><li>Ion Exchange Group: Alkanol Quaternary Ammonium Ion</li><li>Max. Pressure: 3000 psi (207 bar)</li><li>Solvent Compatibility: pH 0 to 14, 0 to 100% HPLC Solvents</li><li>Stationary phase: IonPac AS22</li><li>Format: Standard Bore</li><li>Packing Material: Polymeric</li></ul>

Thermo Scientific Dionex IonPac AS23 IC Columns

Measure trace bromate and other oxyhalides in drinking water, ground water with Thermo Scientific Dionex IonPac AS23 carbonate eluent anion-exchange column. 

Measure trace bromate and other oxyhalides in drinking water, ground water, wastewater, and other diverse sample matrices with the Thermo Scientific™ Dionex™ IonPac™ AS23 carbonate eluent anion-exchange column. The column is specifically designed and recommended for the determination of trace bromate, a toxic byproduct of ozone disinfection, in drinking water matrices. The column's high capacity and selectivity facilitate the determination of bromate in drinking water at the low-μg/L level in the presence of high concentration of chloride, sulfate and carbonate. The Dionex IonPac AS23 columns meet the performance requirements of U.S. EPA Methods 300.0 and 300.1.<h5>Analysis of Trace Level Bromate in Drinking Water</h5><ul><li>Compliance monitoring of common anions and oxyhalides in drinking water as specified in U.S. EPA Method 300.0 and 300.1</li><li>Simple analysis of trace bromate in drinking water matrices using an isocratic carbonate/bicarbonate eluent with suppressed conductivity detection</li><li>Optimized selectivity to ensure low ppb determination of bromate in the presence of very high concentrations of chloride, sulfate, and carbonate</li><li>Separates oxyhalides and common inorganic anions in drinking water, groundwater, wastewater and other diverse sample matrices</li><li>High capacity for the analysis of most drinking water without the use of sample pretreatment or preconcentration</li><li>Solvent compatibility for easy column cleanup after the analysis of samples with hydrophobic components</li></ul><h5>Multiple Formats Available for Application Flexibility</h5><ul><li>4x250mm, 2x250mm analytical columns</li><li>4x50mm, 2x50mm guard columns</li><li>Longer 250mm columns facilitate higher resolution separations of more complex sample matrices</li><li>Smaller i.d. columns provide higher mass sensitivity, eluent and reagent conservation and lower operating costs</li></ul>Note: For simple, accurate eluent preparation, use the Dionex IonPac AS23 Eluent Concentrate.<h4>Specifications</h4><ul><li>Anion Exchange Capacity: 320 μeq</li><li>Crosslinking: 55% DVB</li><li>Column Capacity: 320 μeq</li><li>Column Chemistry: Carbonate/Bicarbonate</li><li>For Use With (Application): Anion Exchange</li><li>Ion Exchange Group: Alkanol Quaternary Ammonium Ion</li><li>Max. Pressure: 3000 psi (207 bar)</li><li>Solvent Compatibility: 0 to 100% HPLC Solvents</li><li>Stationary phase: IonPac AS23</li><li>Format: Standard Bore</li><li>Packing Material: Polymeric</li></ul>

Thermo Scientific Dionex IonPac AS27 IC Columns

Achieve high resolution separations of trace oxyhalides and inorganic anions in drinking water using Thermo Scientific Dionex IonPac AS27 Analytical and Guard columns.

Achieve high resolution separations of trace oxyhalides and inorganic anions in drinking water using Thermo Scientific™ Dionex™ IonPac™ AS27 Analytical and Guard columns. These hydroxide-selective anion-exchange columns allows analysis of most drinking water preserved with ethylenediamine (EDA) without sample pretreatment or preconcentration. The Dionex IonPac AS27 columns meets the performance requirements of US EPA Methods 300.0 and 300.1.<h5>Analyzes Oxyhalides and Inorganic Anions in Drinking Water</h5><ul><li>Optimized for analysis of trace bromate in drinking water preserved with EDA</li><li>High capcity: 220μeq per column (4x250mm)</li><li>Meets or exceeds performance requirements of US EPA Methods 300.0 and 300.1</li><li>Column selectivity is optimized for 30°C operating temperature</li><li>Compatible with organic solvents</li></ul><h5>Choose from Multiple Formats for Application Flexibility</h5><ul><li>4x250mm, 2x250mm, 0.4x250mm analytical columns</li><li>4x50mm, 2x50mm, 0.4x50mm guard columns</li></ul><h4>Specifications</h4><ul><li>Anion Exchange Capacity: 220 μeq</li><li>Crosslinking: 55% DVB</li><li>Column Capacity: 220 μeq</li><li>Column Chemistry: Hydroxide</li><li>For Use With (Application): Anion Exchange</li><li>Ion Exchange Group: Alkanol Quaternary Ammonium Ion</li><li>Max. Pressure: 3000 psi (207 bar)</li><li>Solvent Compatibility: pH 0 to 14, 0 to 100% HPLC Solvents</li><li>Stationary phase: Super Macroporous Resin</li><li>Format: Standard Bore</li><li>Packing Material: Polymeric</li></ul>

Thermo Scientific Dionex IonPac AS28-Fast-4μm IC Columns

Analyze trace inorganic anions and low molecular weight organic acids using the Thermo Scientific Dionex IonPac AS28-Fast-4μm ion chromatography (IC) columns. 

Analyze trace inorganic anions and low molecular weight organic acids using the Thermo Scientific™ Dionex™ IonPac™ AS28-Fast-4μm ion chromatography (IC) columns. Designed specifically for trace level analysis of high purity water from semiconductor and power industries, this column combines the selectivity of the Thermo Scientific™ Dionex™ IonPac™ AS15 column, with fast, high efficiency, and high resolution separations. The Dionex IonPac AS28-Fast-4μm columns uses smaller 4μm resin particles for more efficient separations resulting in more accurate peak integration and more reliable results.<h5>Applications for Dionex IonPac AS28-Fast-4μm IC Columns</h5><ul><li>Separation of common anions and low molecular weight organic acids</li><li>For both high purity water matrices and complex matrices including chemical and petrochemical samples</li><li>Compatible with organic solvents to enhance analyte solubility, modify column selectivity, and facilitate effective column clean-up</li></ul><h5>Superior Performance</h5><ul><li>Improved efficiencies and resolution compared to the Dionex IonPac AS15 column</li><li>Easily transfer applications from the Dionex IonPac AS15 column to the AS28-Fast-4μm column due to the similar selectivity</li></ul><h5>For Use With a High-Pressure IC System</h5>Higher resolution is achieved through the use of smaller resin particles, which produce a column backpressure over 3000 psi at the standard flow rates. A high-pressure IC system, such as the Thermo Scientific™ Dionex™ ICS-5000+ HPIC™ or the Thermo Scientific Dionex ICS-4000 Capillary HPIC system is required to operate this column under standard conditions and take advantage of elevated flow rates and faster run times.<h5>Reduce Operating Costs With Lower Eluent Requirements</h5>The Dionex IonPac AS28-Fast-4μm capillary columns provide the same performance as the standard bore and microbore Dionex IonPac AS28-Fast-4μm columns because they are packed with the same material. Operating costs are reduced because the capillary system operates at only 1% of the eluent flow rate of the standard bore system, requiring less eluent and producing less waste.<h5>Multiple Formats Available</h5><ul><li>4 x 150mm and 2 x 150mm analytical columns</li><li>4 x 30mm and 2 x 30mm analytical guard columns</li><li>0.4 x 150mm capillary columns</li><li>0.4 x 35mm capillary guard columns</li><li>Smaller i.d. columns provide higher mass sensitivity, eluent and reagent conservation, and lower operating costs</li></ul><h4>Specifications</h4><ul><li>Anion Exchange Capacity: 230 μeq</li><li>Crosslinking: 55% DVB</li><li>Column Capacity: 230 μeq</li><li>Column Chemistry: Hydroxide</li><li>For Use With (Application): Anion Exchange</li><li>Ion Exchange Group: Alkanol Quaternary Ammonium Ion</li><li>Max. Pressure: 5000 psi (345 bar)</li><li>Solvent Compatibility: pH 0 to 14, 0 to 100% Acetonitrile, Methanol, and Isopropanol</li><li>Stationary phase: Super Macroporous Ethylvinylbenzene Polymer Cross-Linked with Divinylbenzene</li><li>Format: Standard Bore</li><li>Packing Material: Ethylvinylbenzene/Divinylbenzene</li></ul>

Thermo Scientific Dionex IonPac AS29-Fast-4μm IC Columns

Analyze inorganic anions in high ionic strength matrices using Thermo Scientific™ Dionex™ AS29-Fast-4μm ion chromatography (IC) columns.

Analyze inorganic anions in high ionic strength matrices using Thermo Scientific™ Dionex™ AS29-Fast-4μm ion chromatography (IC) columns. Specifically designed to handle high ionic strength samples, Dionex IonPac AS29-Fast-4μm columns can easily separate the common inorganic anions in less than 10 minutes and can tolerate samples with high or low pH without requiring any pretreatment. They are ideal for analzying drinking water, wastewater, process streams, and scrubber solutions. Use with the Dionex AS29 Eluent Concentrate for fast, convenient, and reliable eluent preparation.<h5>Fast separation of common inorganic anions with excellent resolution</h5><ul><li>Achieve fast, isocratic separation of common inorganic anions in less than 10 minutes.</li><li>Analyze high ionic strength samples without compromising the separation of other analytes.</li><li>Excellent resolution of carbonate away from the common inorganic anions.</li><li>Robust stationary phase can tolerate acidic or basic samples without the need for pretreatment.</li><li>Similar peak efficiency to Dionex IonPac AS22-Fast-4μm column with the same elution order.</li><li>Solvent compatibility permits easy column cleanup after the analysis of samples with hydrophobic components.</li><li>Use with Dionex AS29 Eluent Concentrate for simple and accurate eluent preparation.</li></ul><h5>Use with a High Pressure IC system</h5>Higher resolution is achieved through the use of smaller resin particles, which produce a column backpressure over 3000 psi at the standard flow rates. A high-pressure IC system, such as the Thermo Scientific™ Dionex™ ICS-5000+ HPIC™ system or Thermo Scientific™ Dionex™ Integrion™ HPIC system is required to operate this column under standard conditions.<h4>Specifications</h4><ul><li>Anion Exchange Capacity: 126 μeq</li><li>Crosslinking: 55% DVB</li><li>Column Capacity: 126 μeq</li><li>Column Chemistry: Carbonate/Bicarbonate</li><li>For Use With (Application): Anion Exchange</li><li>Ion Exchange Group: Alkanol Quaternary Ammonium Ion</li><li>Max. Pressure: 5000 psi (345 bar)</li><li>Solvent Compatibility: pH 0 to 14, 0 to 100% Organic HPLC Solvents</li><li>Stationary phase: Super Macroporous Resin</li><li>Format: Standard Bore</li><li>Packing Material: Ethylvinylbenzene/Divinylbenzene</li></ul>

Thermo Scientific Dionex IonPac AS30 IC Columns

Achieve high resolution separations of trace oxyhalides and inorganic anions in drinking water with Thermo Scientific Dionex IonPac AS30 columns.

Achieve high resolution separations of trace oxyhalides and inorganic anions in drinking water with Thermo Scientific™ Dionex™ IonPac™ AS30 columns. The column selectivity has been optimized for trace level analysis of bromate in drinking water preserved with ethylenediamine (EDA). EDA is used as a preservative in some drinking water samples and can cause interference with early eluting peaks. The high capacity of this hydroxide-selective anion-exchange column allows large loop injections for trace analysis (μg/L) without sample pre-treatment or preconcentration.<h5>Superior Chromatographic Performance</h5><ul><li>Optimized for analysis of trace bromate in drinking water preserved with EDA</li><li>High capacity: 477 μeq/column (4 x 250 mm) allows large loop injections for trace analysis</li><li>Column selectivity has been optimized at an operating temperature of 30°C</li><li>Compatible with organic solvents</li></ul><h5>Unique Selectivity for Samples Preserved with Ethylenediamine</h5>The selectivity of the Dionex IonPac AS30 column has been optimized to facilitate quantitation of trace levels of bromate in drinking water containing up to 50 ppm ethylenediamine (EDA). Ethylenediamine is sometimes added to drinking water samples as a preservative and can react with carbonate to produce artifacts that interfere with early eluting analytes such as fluoride. The Dionex IonPac AS30 column minimizes this interference by increasing the separation between fluoride and the EDA carbamate artifact, allowing better integration of the fluoride peak. In comparison to the Dionex IonPac AS19 and IonPac AS27 columns, the Dionex IonPac AS30 column features unique selectivity and much higher column capacity to enable separation of fluoride from the EDA carbamate artifact as well as separation of carbonate and sulfate for better quantification of sulfate.The Dionex IonPac AS30 column is suitable for determining bromate at 5 ppb in a matrix containing up to 50 ppm each of chloride, carbonate, and sulfate. For trace bromate analysis below 5 ppb in higher ionic strength matrices (where chloride levels exceed 50 ppm), the Dionex IonPac AS19 column is recommended. Alternatively, the Dionex IonPac AS27 column is recommended for higher ionic strength matrices preserved with EDA.<h5>Use with a High-Pressure IC system</h5>A high-pressure IC system such as the Thermo Scientific™ Dionex™ ICS-5000+, Dionex ICS-6000, or Dionex Integrion HPIC™ system is required to use this column under standard operating conditions.<h4>Specifications</h4><ul><li>Anion Exchange Capacity: 6 μeq</li><li>Crosslinking: 55% DVB</li><li>Column Capacity: 477 μeq</li><li>Column Chemistry: Hydroxide</li><li>For Use With (Application): Anion Exchange</li><li>Ion Exchange Group: Alkanol Quaternary Ammonium Ion</li><li>Max. Pressure: 5000 psi (345 bar)</li><li>Solvent Compatibility: pH 0 to 14, 0 to 100% HPLC Solvents</li><li>Stationary phase: Super Macroporous Resin</li><li>Format: Standard Bore</li><li>Packing Material: Ethylvinylbenzene/Divinylbenzene</li></ul>

Thermo Scientific Dionex IonPac AS32-Fast-4μm IC Columns

Resolve polythionates, persulfate, polysulfonated aromatics, and aromatic dyes and pigments with the Thermo Scientific Dionex IonPac AS32-Fast-4μm column.

Resolve polythionates, persulfate, polysulfonated aromatics, and aromatic dyes and pigments with the Thermo Scientific™ Dionex™ IonPac™ AS32-Fast-4μm column. The unique selectivity of the Dionex IonPac AS32-Fast-4μm column allows it to resolve trace amounts of perchlorate in matrices containing high levels of chloride and sulfate within ten minutes using simple, isocratic conditions. Compared to similar anion-exchange columns packed with 7-9μm resin particles, Dionex IonPac AS32-Fast-4μm columns produce more efficient peaks, resulting in more accurate integration and more reliable results.<h5>Superior Chromatographic Performance</h5><ul><li>High capacity allows injection of complex matrices without overloading the column while maintaining excellent peak shapes for lower concentration analytes.</li><li>Ultralow hydrophobicity and reduced 150mm column length allow for fast analysis of highly polarizable anions such as perchlorate and iodide with a simple, isocratic eluent.</li><li>Quickly separate disparate concentration ratios (1:1000) of perchlorate in high sulfate matrices using simple, isocratic conditions.</li><li>Separate polythionates such as dithionate, trithionate, and tetrathionate.</li><li>Separate color additives including FD&amp;C Yellow #5, FD&amp;C Yellow #6, FD&amp;C Blue #1, and FD&amp;C Red #40 using a hydroxide gradient.</li></ul><h5>Use with a High-Pressure IC system</h5>A high-pressure IC system, such as the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/410">Thermo Scientific™ Dionex™ ICS-6000 HPIC system</a> or <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/408">Thermo Scientific™ Dionex™ Integrion™ HPIC system</a> is required to operate this column under standard conditions.<h4>Specifications</h4><ul><li>Anion Exchange Capacity: 126 μeq</li><li>Crosslinking: 55% DVB</li><li>Column Capacity: 126 μeq</li><li>Column Chemistry: Hydroxide</li><li>For Use With (Application): Anion Exchange</li><li>Ion Exchange Group: Alkanol Quaternary Ammonium Ion</li><li>Max. Pressure: 5000 psi (345 bar)</li><li>Solvent Compatibility: pH 0 to 14, 0 to 100% HPLC Solvents</li><li>Stationary phase: Super Macroporous Resin</li><li>Format: Standard Bore</li><li>Packing Material: Ethylvinylbenzene/Divinylbenzene</li></ul>

Thermo Scientific Dionex IonPac AS4A-SC IC Columns

For fast, isocratic separation of inorganic anions in environmental waters, the Thermo Scientific Dionex IonPac AS4A-SC column was the preferred column for many years. 

For fast, isocratic separation of inorganic anions in environmental waters, the Thermo Scientific™ Dionex™ IonPac™ AS4A-SC column was the preferred column for many years. It was first introduced in 1984 and is the specified column in U.S. EPA Method 300.0 (A). However, the advanced, high-capacity Dionex IonPac AS22 Carbonate Eluent Anion-Exchange column is recommended for fast analysis of inorganic anions in diverse matrices when using carbonate eluents.<h5>Fast Isocratic Separation of Inorganic Anions in Simple Matrices</h5><ul><li>Compliance monitoring of common anions in drinking water as specified in U.S. EPA Methods 300.0 (A)</li><li>Separation of common inorganic anions in 10 minutes in water, food and beverages, and other diverse sample matrices</li><li>Compatible with sodium tetraborate gradient to optimize difficult separations</li><li>Solvent compatibility for easy column cleanup after the analysis of samples with hydrophobic components</li></ul><h5>Multiple Formats Available for Application Flexibility</h5><ul><li>4×250mm, 2x250mm analytical columns</li><li>4×50mm, 2x50mm guard columns</li><li>Longer 250mm columns facilitate higher resolution separations of more complex sample matrices</li><li>Smaller i.d. columns provide higher mass sensitivity, eluent and reagent conservation and lower operating costs</li></ul>Note: Use with the Dionex IonPac AS4A Eluent Concentrate for simple, accurate eluent preparation.<h4>Specifications</h4><ul><li>Column Capacity: 20 μeq</li><li>Column Chemistry: Carbonate/Bicarbonate</li><li>Crosslinking: 0.5% DVB</li><li>For Use With (Application): Separation of Inorganic Anions, Anion Exchange</li><li>Ion Exchange Group: Alkanol Quaternary Ammonium Ion</li><li>Max. Pressure: 4000 psi (275 bar)</li><li>Solvent Compatibility: pH 0 to 14, 0 to 100% HPLC Solvents</li><li>Stationary phase: IonPac AS4A-SC</li><li>Packing Material: Ethylvinylbenzene/Divinylbenzene</li></ul>

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