<p><strong>In the week of September 14, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>6 000 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Agilent Technologies: Using Targeted Methylation Sequencing to Bring Epigenetic Biomarkers to the Clinic</strong></h5><ul><li>Mo, 14.9.2026 18:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6497">Registration</a></li></ul><p>Discover how native-read nanopore sequencing and Agilent SureSelect targeting can translate epigenetic biomarker discoveries into high-depth methylation assays for clinical research.</p><h5><strong>2. Thermo Fisher Scientific: Polymeric, porous, monodisperse particle development and application to reversed-phase separations of biological molecules</strong></h5><ul><li>Tu, 15.9.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6473">Registration</a></li></ul><p>Learn how monodisperse polymeric particle architecture can optimize reversed-phase separations of biological molecules, including oligonucleotides and RNA.</p><h5><strong>3. Waters Corporation: LC Coffee Breaks: Strategies to Identify and Reduce Carryover</strong></h5><ul><li>Tu, 15.9.2026 14:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6418">Registration</a></li></ul><p>Learn practical strategies to identify and eliminate LC carryover, including common sources, troubleshooting techniques, and proven solutions for more robust and reproducible analyses.</p><h5><strong>4. C&amp;EN: Fast Track Closed-Loop Experimentation with AI-Driven Automation on Stuntman</strong></h5><ul><li>Tu, 15.9.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6472">Registration</a></li></ul><p>Discover how the Stuntman platform integrates AI and laboratory automation to enable closed-loop experimentation and accelerate research and discovery workflows.</p><h5><strong>5. Thermo Fisher Scientific: Rethinking what's possible</strong></h5><ul><li>Tu, 15.9.2026 18:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6536">Registration</a></li></ul><p>Explore how advances in proteomics, systems biology, and computational approaches are reshaping precision health and opening new ways to study disease, biology, and One Health.</p><h5><strong>6. Thermo Fisher Scientific: LC-MS/MS determination of PFAS in wild Baltic grey seal plasma for the assessment of health and developmental parameters</strong></h5><ul><li>We, 16.9.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6474">Registration</a></li></ul><p>Learn how Hypersil GOLD C18, delay columns, isotope dilution, and LC-MS/MS support PFAS biomonitoring in wildlife plasma and assessment of health and developmental parameters.</p><h5><strong>7. SelectScience: Moving beyond reactive compliance by building inspection-ready laboratories</strong></h5><ul><li>We, 16.9.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6501">Registration</a></li></ul><p>Learn how AI and cloud-based informatics can strengthen data integrity, compliance, and inspection readiness in modern analytical laboratories.</p><h5><strong>8. Thermo Fisher Scientific: Powering Discovery at Scale</strong></h5><ul><li>We, 16.9.2026 18:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6537">Registration</a></li></ul><p>Discover how high-throughput proteomics, advanced analytical performance, and computational biology enable larger translational studies and deeper biological insights.</p><h5><strong>9. Agilent Technologies: Increasing LC/MS Productivity: Deciding Which High Throughput Solution is Right for You</strong></h5><ul><li>We, 16.9.2026 18:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6494">Registration</a></li></ul><p>Learn how to select the right high-throughput LC/MS solution for your laboratory by balancing analytical performance, throughput, and scalability.</p><h5><strong>10. Agilent Technologies: Accelerating Biopharmaceutical Development with the Agilent Cary 3500 UV-Vis Spectrophotometer</strong></h5><ul><li>Th, 17.9.2026 07:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6507">Registration</a></li></ul><p>Explore how the Agilent Cary 3500 UV-Vis spectrophotometer supports biopharmaceutical characterization from development through routine quality control.</p><h5><strong>11. Thermo Fisher Scientific: EFOX: Smarter Environmental Workflows for PFAS and Emerging Contaminants</strong></h5><ul><li>Th, 17.9.2026 08:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6508">Registration</a></li></ul><p>Discover how the Thermo Scientific EFOX platform streamlines PFAS and emerging contaminant analysis for modern environmental laboratories.</p><h5><strong>12. Thermo Fisher Scientific: Practical aspects and advantages of coupling ion exchange chromatography to mass spectrometry for biotherapeutic analysis</strong></h5><ul><li>Th, 17.9.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6475">Registration</a></li></ul><p>Learn how direct IEX-MS coupling with pH-gradient elution and volatile buffers can streamline biotherapeutic characterization.</p><h5><strong>13. Thermo Fisher Scientific: Building the Modern Pharma QA/QC Laboratory</strong></h5><ul><li>Th, 17.9.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6510">Registration</a></li></ul><p>Discover a practical roadmap for moving pharmaceutical QA/QC laboratories from legacy systems toward connected digital environments with stronger compliance and efficiency.</p><h5><strong>14. Waters Corporation: Excipients in Biopharma – Polysorbate Analysis</strong></h5><ul><li>Th, 17.9.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6509">Registration</a></li></ul><p>Learn how HPLC with charged aerosol detection simplifies characterization of Polysorbate 20 and 80 in biopharmaceutical excipient analysis.</p><h5><strong>15. SelectScience: Master EV profiling with Leprechaun – A case study from North Texas Eye Research Institute</strong></h5><ul><li>Th, 17.9.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6506">Registration</a></li></ul><p>Explore advanced extracellular vesicle profiling and discover how the Leprechaun platform streamlines EV characterization for research applications.</p><h5><strong>16. Agilent Technologies: Comprehensive SEC Solutions: Performance and Application Insights Across Agilent’s SEC Columns</strong></h5><ul><li>Th, 17.9.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6350">Registration</a></li></ul><p>Optimize size exclusion chromatography and learn how Agilent SEC columns support high-resolution aggregate analysis and polymer profiling in R&amp;D and quality control.</p><h5><strong>17. Thermo Fisher Scientific: Turning Discovery into Precision Health</strong></h5><ul><li>Th, 17.9.2026 18:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6538">Registration</a></li></ul><p>Discover how translational proteomics bridges molecular discovery and clinical implementation, accelerating biomarker development and precision health research.</p><h5><strong>18. SelectScience: High-resolution oligonucleotide analysis with capillary gel electrophoresis</strong></h5><ul><li>Fr, 18.9.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6512">Registration</a></li></ul><p>Learn how capillary gel electrophoresis delivers single-nucleotide resolution for high-resolution oligonucleotide characterization.</p><h5><strong>19. SelectScience: Reliable biopharma QC starts with MAM system readiness</strong></h5><ul><li>Fr, 18.9.2026 17:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6513">Registration</a></li></ul><p>Learn how automated LC/MS system readiness checks support reliable multi-attribute method workflows and consistent results in GMP biopharmaceutical QC environments.</p>

Webinars LabRulezLCMS Week 38/2026

<p><strong>In the week from September 14, the following webinars await you in the field of atomic and molecular spectroscopy and spectrometry</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>6 000 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Technology Networks: Automating Microplastic Analysis To Support Water Quality Research</strong></h5><ul><li>Tu, 15.9.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6471">Registration</a></li></ul><p>Join this webinar on automated microplastic analysis and learn how automated sample preparation can improve workflow efficiency and support water quality research.</p><h5><strong>2. C&amp;EN: Fast Track Closed-Loop Experimentation with AI-Driven Automation on Stuntman</strong></h5><ul><li>Tu, 15.9.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6472">Registration</a></li></ul><p>Discover how the Stuntman platform integrates AI and laboratory automation to enable closed-loop experimentation and accelerate research and discovery workflows.</p><h5><strong>3. Agilent Technologies: ICP-OES Preventive Maintenance: What to Clean, How, and When</strong></h5><ul><li>Tu, 15.9.2026 21:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6413">Registration</a></li></ul><p>Learn key ICP-OES cleaning procedures and maintenance schedules to prevent signal drift, instability, and unnecessary instrument downtime.</p><h5><strong>4. Bruker: From Pit to Port: XRF Solutions for Base Metal Mining Operations</strong></h5><ul><li>We, 16.9.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6502">Registration</a></li></ul><p>Explore how EDXRF and WDXRF support base metal mining operations, from grade control and process monitoring to quality assurance across the production chain.</p><h5><strong>5. Bruker: AI-Assisted Analysis of Viscoelasticity with AFM</strong></h5><ul><li>We, 16.9.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6503">Registration</a></li></ul><p>Learn how machine learning and the ViscoDirect module accelerate AFM-based viscoelasticity analysis and cell nanorheology workflows.</p><h5><strong>6. Anton Paar: Extreme-Temperature Stability of MXenes Revealed by In-Situ High-Temperature XRD</strong></h5><ul><li>We, 16.9.2026 18:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6429">Registration</a></li></ul><p>Discover how non-ambient XRD chambers enable in-situ characterization of MXene structural dynamics at temperatures up to 1200 °C.</p><h5><strong>7. Agilent Technologies: Accelerating Biopharmaceutical Development with the Agilent Cary 3500 UV-Vis Spectrophotometer</strong></h5><ul><li>Th, 17.9.2026 07:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6507">Registration</a></li></ul><p>Explore how the Agilent Cary 3500 UV-Vis spectrophotometer supports biopharmaceutical characterization from development through routine quality control.</p><h5><strong>8. Agilent Technologies: Landscape of Standardization and Overview of Analytical Techniques Used for Microplastics Analysis</strong></h5><ul><li>Th, 17.9.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6539">Registration</a></li></ul><p>Explore current trends in microplastics analysis, including analytical standards, molecular spectroscopy, Py-GC/MS, TD-GC/MS, and future developments for reliable characterization.</p><h5><strong>9. SelectScience: Master EV profiling with Leprechaun – A case study from North Texas Eye Research Institute</strong></h5><ul><li>Th, 17.9.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6506">Registration</a></li></ul><p>Explore advanced extracellular vesicle profiling and discover how the Leprechaun platform streamlines EV characterization for research applications.</p><h5><strong>10. Agilent Technologies: Analysis of High Purity Titanium Using an Agilent 9500 ICP-QQQ</strong></h5><ul><li>Th, 17.9.2026 20:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6414">Registration</a></li></ul><p>Learn how the Agilent 9500 ICP-QQQ overcomes titanium matrix effects to enable trace impurity analysis in high-purity titanium.</p>

Webinars LabRulezICPMS Week 38/2026

ISC 2026 - Day 4

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The International Symposium on Chromatography (ISC) represents the oldest conference series focussing on separation science. ISC symposia have been organised since 1956 in each even year. ISC is internationally recognised as one of the premier meeting series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.

International Symposium on Chromatography - (ISC)

KNAUER Eurospher II 100 C8 & C8A (U)HPLC Columns (USP L7)

C8 is a silica gel based stationary phase in reversed phase mode with alternative selectivity compared to C18 phases. 

<p class="cke-user_style-important"><strong>Classical and hydrophilic/aqueous C8 columns for HPLC and UHPLC (USP L7)</strong></p><p>C8 is a <strong>silica gel based stationary phase</strong> in <strong>reversed phase</strong> mode with alternative selectivity compared to C18 phases. Compared to C18 phases, these columns show <strong>less retention</strong> due to the <strong>lower hydrophobicity</strong>.</p><ul><li>Ultra pure, spherical high performance HPLC phase based on silica gel</li><li>Monomeric C8 (Octyl) modification, standard endcapping, with 10 % carbon content (~ 50 % endcapping)</li></ul><h5><strong>Properties</strong></h5><ul><li><strong>Separation mechanism</strong>: Hydrophobic interaction(lower compared to C18 phases)</li></ul><h5><strong>Key features</strong></h5><ul><li>Classical C8 phase, standard 50 % endcapping and 10 % carbon load, outstanding mechanical and chemical stability, suited for analytical as well as semi preparative and preparative applications.</li></ul><h5><strong>Recommended application areas</strong></h5><ul><li>Similar selectivity to C18 phase but less retention due to the lower hydrophobicity; useful for determination of:<ul><li>Water soluble vitamins</li><li>Steroids</li><li>Catecholamines</li><li>Sedatives</li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_C8_rp_non_endcapped_3f7727d3fd.webp" alt="KNAUER: C8 RP Non-endcapped" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_C8_rp_non_endcapped_3f7727d3fd.webp 148w," sizes="100vw" width="304" height="321"></p><p><strong>Hydrophilic C8A phase</strong> offers an alternative, more polar selectivity. It is suited for highly polar reversed phase applications with up to 100 % aqueous mobile phases.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_C8_A_e1f371165e.webp" alt="KNAUER: C8A" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_C8_A_e1f371165e.webp 198w," sizes="100vw" width="473" height="373"></p><blockquote><p><strong>Tip: Caution! </strong>Even for short term storage all buffer solutions have to be rinsed from the column to prevent crystallization effects.</p></blockquote><p class="cke-user_style-important"><strong>Stationary phases Eurospher II 100 C8 and C8A are provided in these column types: </strong>analytical, semi-preparative and preparative.</p><p><strong>Eurospher II 100 C8 and C8A Analytical Columns </strong>are available with these parameters:</p><ul><li><strong>Column length (mm): </strong>50, 100, 150, 250</li><li><strong>Column ID (mm): </strong>2, 3, 4, 4.6</li><li><strong>Particle size (µm): </strong>2, 3, 5</li></ul><p><strong>Eurospher II 100 C8 and C8A Semi-Preparative Standard Columns </strong>are available with these parameters:</p><ul><li><strong>Column length (mm): </strong>30,<strong> </strong>50, 125, 150, 250</li><li><strong>Column ID (mm): </strong>8, 16</li><li><strong>Particle size (µm): </strong>5, 10</li></ul><h3><strong>KNAUER (U)HPLC Columns</strong></h3><p><strong>Widest range of HPLC and UHPLC columns for all separation modes and applications.</strong></p><ul><li><strong>Safe Investment</strong>: Robust and reliable columns for attractive price&nbsp;</li><li><strong>Separation Mode</strong>: Reversed Phase, HILIC, Normale</li><li><strong>Simplicity: </strong>Easy purchase via our online store with fast delivery</li><li><strong>Application: </strong>All-rounder for research or highly specialised for high-throughput analysis? We have the best column for your requirements.</li><li><strong>Flexibility:</strong> Wide selection of column and particle sizes, for (U)HPLC applications up to 1000 bar</li></ul><p><strong>KNAUER offers the perfect column for any small molecule separation.</strong> No matter which separation mode, molecule and the mobile phase, we offer a suitable (U)HPLC stationary phase. Choose from a wide range of modifications, particle sizes and column dimensions to suit your application. To elongate the lifetime of your column, you can choose to use a suitable precolumn.</p><figure class="image"><img style="aspect-ratio:787/674;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_KNAUER_U_HPLC_C8_and_C8_A_Columns_083c60f401.webp" alt="KNAUER: KNAUER (U)HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_KNAUER_U_HPLC_C8_and_C8_A_Columns_083c60f401.webp 182w," sizes="100vw" width="787" height="674"></figure>

KNAUER AppliChrom® ABOA Cat-Phil SEC Columns

Interaction-free pure GPC & SEC base material with focus on polycationic, polyelectrolytic or funktional amine analytes

<p class="cke-user_style-important"><strong>AppliChrom® SEC columns for neutral, anionic and additional cationic polymers</strong></p><h4><strong>Properties:</strong></h4><ul><li>Interaction-free pure GPC &amp; SEC base material with focus on polycationic, polyelectrolytic or funktional amine analytes</li><li>No salt addition is needed to surpress surface adsorption</li><li>No ion exclusion phenomena</li><li>Possible to be used in universal calibration &amp; accurate molecular weight determination even for small polycationic molecules</li><li><strong>Temperature stability between:</strong> 10–90 °C</li><li><strong>Stable with:</strong> pH 2–10</li><li><strong>Simple, reliable and robust GPC &amp; SEC calibration</strong> from dextrans, pullulans et al.</li><li><strong>Low column bleed </strong>for low detector gain for improved refractive index, light scattering or viscosity detection</li><li><strong>Large volume</strong> and optimized mass transfer for polymers with optimized resolution</li><li><strong>Low cost due to long column lifetime</strong> - result of the combination of optimized particle and packing technology</li><li><strong>Recommended eluents:</strong> H₂O, buffers</li></ul><h4><strong>Applications</strong></h4><p><strong>Water soluble analytes like:</strong></p><ul><li>polycations, polyamines (chitosanes), polyethylenoxides, polysaccharides, polyanions (heparins, pectins), polyethylene oxide (PEO), polyethylene glycol (PEG), Ppolyethyleneimins (PEI), polyvinylimidazole</li></ul><h3><strong>GPC &amp; SEC Columns</strong></h3><p><strong>GPC &amp; SEC systems offer solutions for various applications and laboratories</strong></p><p>Gel permeation chromatography (GPC) and size exclusion chromatography (SEC) separate analytes by size using special columns with a porous matrix. The basic separation principle is based on the fact that smaller molecules penetrate these pores more strongly and therefore move more slowly through the matrix than larger molecules. GPC/SEC is often used as gentle and powerful purification technique or for quality control in research and development. Typical target molecules are polymers, dissolved in organic solvents for GPC, and macro biomolecules like proteins, antibodies or polysaccharides, dissolved in aqueous eluents for SEC.</p><p>Separation is facilitated by selecting optimal particle and pore sizes. Therefore we offer a <strong>wide range of pore sizes</strong> (35, 100, 150, 250, 350, 500, 1000, 1500, 10E5, 10E6, 10E7 Å) and <strong>several particle sizes</strong> (5, 7, 10, 12, 20 µm) as a choice to customize for every application task.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Size_Exclusion_principle_2a432edcc1.webp" alt="KNAUER: Size Exclusion - principle" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Size_Exclusion_principle_2a432edcc1.webp 228w," sizes="100vw" width="1024" height="700"></p><h3><strong>KNAUER AppliChrom® ABOA columns for aqueous SEC, organic GPC, and special applications</strong></h3><ul><li>AppliChrom® ABOA StyDiViBe Series GPC-columns for GPC analysis of organic molecules using THF, toluene, chloroform.</li><li>AppliChrom ABOA SuperOH-P Series for aqueous GPC/SEC-analysis of mainly neutral and anionic polymers like heparins, polysaccharides, PEO,<br>PEG,....</li><li>AppliChrom® ABOA DMSO-Phil-P Series for DMSO GPC/SEC analysis (humic substances, UF/MUF-glue, p-NIPA, many polycondensates, protein from pea or leguminosae,...)</li><li>AppliChrom® ABOA DMAc-Phil-P Series for GPC/ SEC analysis in DMAc, DMF, NMP. Good for many medium polar substances, polyoxazolines, lignin,...</li><li>AppliChrom® ABOA Cat-Phil-P Series for aqueous GPC/SEC-analysis of special aminic and polycationic polymers like p-DADMAc, PEI/ polyethylenimin, chitosan, copolymers with polyaminofunctions, but also useful for polyanions or neutral polymers like heparin or PEO/PEG, dextran or pullulan.</li><li><strong>Seperation Ranges: </strong>100–50 000 000 Da</li></ul><h4><strong>You decide between classical GPC/SEC columns with conventional mixed pore technology or AppliChrom® BPT technology:</strong></h4><p>Separate oligomers and polymers with the new GPC media line from AppliChrom® BPT synthesis technology for large range, high resolution separations with increased accuracy in calibration (patent pending).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg" alt="KNAUER: AppliChrom® ABOA columns." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg 245w," sizes="100vw" width="1379" height="718"></p>

KNAUER AppliChrom® ABOA StyDiViBe GPC Columns

Organic polystyrene-divinylbenzene copolymer base material with high porosity for high resolution

<p class="cke-user_style-important"><strong>AppliChrom® GPC columns for GPC analysis of organic molecules with THF, toluene, chloroform</strong></p><h4><strong>Properties:</strong></h4><ul><li>Organic polystyrene-divinylbenzene copolymer base material with high porosity for high resolution</li><li><strong>High pressure stability: </strong>up to 150 bar</li><li><strong>Temperature stable:</strong> between 10-145 °C</li><li><strong>GPC range:</strong> from 0.1 up to 50 000 kDa</li><li><strong>Low column bleed</strong> for low detector noise</li><li><strong>High purity</strong> to eliminate adsorptive effects and combine pure GPC with long lifetime</li><li><strong>Large calibration ranges</strong> for several decades due to the unique process of BPT technology</li><li>Range of individual porosities that can be used individually or in combination</li><li><strong>Optimized for: </strong>1 ml/min flow rate for 8 mm ID columns</li><li><strong>High level of reproducibility</strong> and peak precision due to the advanced packing procedure</li><li><strong>Recommended eluents:</strong> THF, Toluene, CHCl₃, dichlorbenzene, hexane/EtOAc (10:90, V:V)</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Appli_Chrom_ABOA_Sty_Di_Vi_Be_P_Molecular_sizes_range_ca9a682d2e.jpg" alt="KNAUER: AppliChrom® ABOA StyDiViBe-P - Molecular sizes range" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Appli_Chrom_ABOA_Sty_Di_Vi_Be_P_Molecular_sizes_range_ca9a682d2e.jpg 235w," sizes="100vw" width="1072" height="712"></p><h4><strong>Applications</strong></h4><p><strong>Hydrophobic oligomers or polymers like:</strong></p><ul><li>PVC, PMMA, polystyrene, epoxy resins, polyesters, bisphenol A resins</li></ul><h3><strong>GPC &amp; SEC Columns</strong></h3><p><strong>GPC &amp; SEC systems offer solutions for various applications and laboratories</strong></p><p>Gel permeation chromatography (GPC) and size exclusion chromatography (SEC) separate analytes by size using special columns with a porous matrix. The basic separation principle is based on the fact that smaller molecules penetrate these pores more strongly and therefore move more slowly through the matrix than larger molecules. GPC/SEC is often used as gentle and powerful purification technique or for quality control in research and development. Typical target molecules are polymers, dissolved in organic solvents for GPC, and macro biomolecules like proteins, antibodies or polysaccharides, dissolved in aqueous eluents for SEC.</p><p>Separation is facilitated by selecting optimal particle and pore sizes. Therefore we offer a <strong>wide range of pore sizes</strong> (35, 100, 150, 250, 350, 500, 1000, 1500, 10E5, 10E6, 10E7 Å) and <strong>several particle sizes</strong> (5, 7, 10, 12, 20 µm) as a choice to customize for every application task.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Size_Exclusion_principle_2a432edcc1.webp" alt="KNAUER: Size Exclusion - principle" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Size_Exclusion_principle_2a432edcc1.webp 228w," sizes="100vw" width="1024" height="700"></p><h3><strong>KNAUER AppliChrom® ABOA columns for aqueous SEC, organic GPC, and special applications</strong></h3><ul><li>AppliChrom® ABOA StyDiViBe Series GPC-columns for GPC analysis of organic molecules using THF, toluene, chloroform.</li><li>AppliChrom ABOA SuperOH-P Series for aqueous GPC/SEC-analysis of mainly neutral and anionic polymers like heparins, polysaccharides, PEO,<br>PEG,....</li><li>AppliChrom® ABOA DMSO-Phil-P Series for DMSO GPC/SEC analysis (humic substances, UF/MUF-glue, p-NIPA, many polycondensates, protein from pea or leguminosae,...)</li><li>AppliChrom® ABOA DMAc-Phil-P Series for GPC/ SEC analysis in DMAc, DMF, NMP. Good for many medium polar substances, polyoxazolines, lignin,...</li><li>AppliChrom® ABOA Cat-Phil-P Series for aqueous GPC/SEC-analysis of special aminic and polycationic polymers like p-DADMAc, PEI/ polyethylenimin, chitosan, copolymers with polyaminofunctions, but also useful for polyanions or neutral polymers like heparin or PEO/PEG, dextran or pullulan.</li><li><strong>Seperation Ranges: </strong>100–50 000 000 Da</li></ul><h4><strong>You decide between classical GPC/SEC columns with conventional mixed pore technology or AppliChrom® BPT technology:</strong></h4><p>Separate oligomers and polymers with the new GPC media line from AppliChrom® BPT synthesis technology for large range, high resolution separations with increased accuracy in calibration (patent pending).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg" alt="KNAUER: AppliChrom® ABOA columns." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg 245w," sizes="100vw" width="1379" height="718"></p>

KNAUER AppliChrom® ABOA DMAc-Phil GPC Columns

Organic polymer base material with high porosity for high resolution

<p class="cke-user_style-important"><strong>AppliChrom® GPC columns for organic GPC analyses in DMAc or DMF</strong></p><h4><strong>Properties:</strong></h4><ul><li>Organic polymer base material with high porosity for high resolution</li><li>Wide range of porosities and particle sizes</li><li><strong>Pressure stability:</strong> up to 150 bar</li><li><strong>Stable between: </strong>35-145 °C</li><li><strong>Excellent reproducibility</strong> and resolution due to the advanced packing procedure</li><li><strong>High purity</strong> to eliminate adsorptive effects and combine pure GPC with long lifetime</li><li><strong>GPC range from:</strong> 100-1 000 000 Da</li><li><strong>Optimal for: </strong>0.5 ml/min flow rates with 8 mm ID colums</li><li><strong>Recommended eluents: </strong>DMAc, DMF, NMP, LiBr additive</li></ul><h4><strong>Applications</strong></h4><p><strong>Slightly polar analytes like:</strong></p><ul><li>PMMA, polyacryl nitril (PAN), acrylnitrile butadiene styrene (ABS), cellulose, and all DMF and DMAc soluble polymers</li></ul><h3><strong>GPC &amp; SEC Columns</strong></h3><p><strong>GPC &amp; SEC systems offer solutions for various applications and laboratories</strong></p><p>Gel permeation chromatography (GPC) and size exclusion chromatography (SEC) separate analytes by size using special columns with a porous matrix. The basic separation principle is based on the fact that smaller molecules penetrate these pores more strongly and therefore move more slowly through the matrix than larger molecules. GPC/SEC is often used as gentle and powerful purification technique or for quality control in research and development. Typical target molecules are polymers, dissolved in organic solvents for GPC, and macro biomolecules like proteins, antibodies or polysaccharides, dissolved in aqueous eluents for SEC.</p><p>Separation is facilitated by selecting optimal particle and pore sizes. Therefore we offer a <strong>wide range of pore sizes</strong> (35, 100, 150, 250, 350, 500, 1000, 1500, 10E5, 10E6, 10E7 Å) and <strong>several particle sizes</strong> (5, 7, 10, 12, 20 µm) as a choice to customize for every application task.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Size_Exclusion_principle_2a432edcc1.webp" alt="KNAUER: Size Exclusion - principle" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Size_Exclusion_principle_2a432edcc1.webp 228w," sizes="100vw" width="1024" height="700"></p><h3><strong>KNAUER AppliChrom® ABOA columns for aqueous SEC, organic GPC, and special applications</strong></h3><ul><li>AppliChrom® ABOA StyDiViBe Series GPC-columns for GPC analysis of organic molecules using THF, toluene, chloroform.</li><li>AppliChrom ABOA SuperOH-P Series for aqueous GPC/SEC-analysis of mainly neutral and anionic polymers like heparins, polysaccharides, PEO,<br>PEG,....</li><li>AppliChrom® ABOA DMSO-Phil-P Series for DMSO GPC/SEC analysis (humic substances, UF/MUF-glue, p-NIPA, many polycondensates, protein from pea or leguminosae,...)</li><li>AppliChrom® ABOA DMAc-Phil-P Series for GPC/ SEC analysis in DMAc, DMF, NMP. Good for many medium polar substances, polyoxazolines, lignin,...</li><li>AppliChrom® ABOA Cat-Phil-P Series for aqueous GPC/SEC-analysis of special aminic and polycationic polymers like p-DADMAc, PEI/ polyethylenimin, chitosan, copolymers with polyaminofunctions, but also useful for polyanions or neutral polymers like heparin or PEO/PEG, dextran or pullulan.</li><li><strong>Seperation Ranges: </strong>100–50 000 000 Da</li></ul><h4><strong>You decide between classical GPC/SEC columns with conventional mixed pore technology or AppliChrom® BPT technology:</strong></h4><p>Separate oligomers and polymers with the new GPC media line from AppliChrom® BPT synthesis technology for large range, high resolution separations with increased accuracy in calibration (patent pending).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg" alt="KNAUER: AppliChrom® ABOA columns." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg 245w," sizes="100vw" width="1379" height="718"></p>

KNAUER AppliChrom® ABOA DMSO-Phil GPC Columns

AppliChrom® DMSO-PHIL optimized for DMSO-GPC applications from an interaction-free pure GPC base material

<p class="cke-user_style-important"><strong>AppliChrom® DMSO-PHIL optimized for DMSO-GPC applications</strong></p><h4><strong>Properties:</strong></h4><ul><li>Interaction-free pure GPC base material</li><li><strong>Temperature stabel between:</strong> 40-90 °C (145 °C) depending on application</li><li><strong>Simple, reliable and robust </strong>GPC &amp; SEC calibration from dextrans, pullulans et al.</li><li><strong>Low column bleed</strong> for low detector gain for improved refractive index, light scattering or viscosity detection</li><li><strong>12 µm particle technology</strong> for low back pressure</li><li>Tolerates traces of water and NaNO₃ additve</li><li><strong>Large volume and optimized mass transfer</strong> for polymers with optimized resolution</li><li><strong>Low cost due to long column lifetime</strong> - result of the combination of optimized particle and packing technology</li><li><strong>Recommended eluents:</strong> DMSO, 0.075 M NaNO₃</li></ul><h4><strong>Applications</strong></h4><p><strong>Polar, not water soluble oligomers or polymers like:</strong></p><ul><li>polysaccharides, lignin, amylose, starch, humic acids, polyvenyl pyridin, PNIPA, polyvinyl alcohol (PVA), melamin-urea-formaldehyd resin (MUF) or urea-formaldehyd resin (UF)</li></ul><h3><strong>GPC &amp; SEC Columns</strong></h3><p><strong>GPC &amp; SEC systems offer solutions for various applications and laboratories</strong></p><p>Gel permeation chromatography (GPC) and size exclusion chromatography (SEC) separate analytes by size using special columns with a porous matrix. The basic separation principle is based on the fact that smaller molecules penetrate these pores more strongly and therefore move more slowly through the matrix than larger molecules. GPC/SEC is often used as gentle and powerful purification technique or for quality control in research and development. Typical target molecules are polymers, dissolved in organic solvents for GPC, and macro biomolecules like proteins, antibodies or polysaccharides, dissolved in aqueous eluents for SEC.</p><p>Separation is facilitated by selecting optimal particle and pore sizes. Therefore we offer a <strong>wide range of pore sizes</strong> (35, 100, 150, 250, 350, 500, 1000, 1500, 10E5, 10E6, 10E7 Å) and <strong>several particle sizes</strong> (5, 7, 10, 12, 20 µm) as a choice to customize for every application task.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Size_Exclusion_principle_2a432edcc1.webp" alt="KNAUER: Size Exclusion - principle" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Size_Exclusion_principle_2a432edcc1.webp 228w," sizes="100vw" width="1024" height="700"></p><h3><strong>KNAUER AppliChrom® ABOA columns for aqueous SEC, organic GPC, and special applications</strong></h3><ul><li>AppliChrom® ABOA StyDiViBe Series GPC-columns for GPC analysis of organic molecules using THF, toluene, chloroform.</li><li>AppliChrom ABOA SuperOH-P Series for aqueous GPC/SEC-analysis of mainly neutral and anionic polymers like heparins, polysaccharides, PEO,<br>PEG,....</li><li>AppliChrom® ABOA DMSO-Phil-P Series for DMSO GPC/SEC analysis (humic substances, UF/MUF-glue, p-NIPA, many polycondensates, protein from pea or leguminosae,...)</li><li>AppliChrom® ABOA DMAc-Phil-P Series for GPC/ SEC analysis in DMAc, DMF, NMP. Good for many medium polar substances, polyoxazolines, lignin,...</li><li>AppliChrom® ABOA Cat-Phil-P Series for aqueous GPC/SEC-analysis of special aminic and polycationic polymers like p-DADMAc, PEI/ polyethylenimin, chitosan, copolymers with polyaminofunctions, but also useful for polyanions or neutral polymers like heparin or PEO/PEG, dextran or pullulan.</li><li><strong>Seperation Ranges: </strong>100–50 000 000 Da</li></ul><h4><strong>You decide between classical GPC/SEC columns with conventional mixed pore technology or AppliChrom® BPT technology:</strong></h4><p>Separate oligomers and polymers with the new GPC media line from AppliChrom® BPT synthesis technology for large range, high resolution separations with increased accuracy in calibration (patent pending).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg" alt="KNAUER: AppliChrom® ABOA columns." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg 245w," sizes="100vw" width="1379" height="718"></p>

KNAUER AppliChrom® ABOA HFIP-Phil GPC Columns

AppliChrom® GPC columns for separations in HFIP with broad pore technology for GPC for analytes with challenging solution behavior

<p class="cke-user_style-important"><strong>AppliChrom® GPC columns for separations in HFIP</strong></p><h4><strong>Properties:</strong></h4><ul><li>Broad pore technology for GPC for analytes with challenging solution behavior</li><li><strong>Separation range beween:</strong> 0.1–50 000 kDa</li><li>Range of individual porosities that can be used individually or in combination</li><li><strong>Temperature stable between:</strong> 35–145 °C</li><li><strong>Long column lifetime</strong> - result of the combination of optimized particle and packing technology</li><li><strong>Low column bleed</strong> for low detector noise</li><li><strong>Excellent reproducibility and resolution</strong> due to the advanced packing procedure</li><li><strong>Calibration standard:</strong> PMMA or PEO &nbsp;</li><li><strong>Recommended eluents</strong>: Hexafluoroisopropanol (HFIP), 0.1 % KTFa as modifier</li></ul><h4><strong>Applications</strong></h4><p><strong>Crystalline highperformance polymers like:&nbsp;</strong></p><ul><li>polyamides (PA), polyethylen terepthalate (PET), polybutylen terepthalate (PBT), polylactic acid (PLA), polyamid 6 (PA6), polycaprolactam, paraformaldehyde, polyoxymethylene (POM), polyacetal, several polyoxazolines</li></ul><h3><strong>GPC &amp; SEC Columns</strong></h3><p><strong>GPC &amp; SEC systems offer solutions for various applications and laboratories</strong></p><p>Gel permeation chromatography (GPC) and size exclusion chromatography (SEC) separate analytes by size using special columns with a porous matrix. The basic separation principle is based on the fact that smaller molecules penetrate these pores more strongly and therefore move more slowly through the matrix than larger molecules. GPC/SEC is often used as gentle and powerful purification technique or for quality control in research and development. Typical target molecules are polymers, dissolved in organic solvents for GPC, and macro biomolecules like proteins, antibodies or polysaccharides, dissolved in aqueous eluents for SEC.</p><p>Separation is facilitated by selecting optimal particle and pore sizes. Therefore we offer a <strong>wide range of pore sizes</strong> (35, 100, 150, 250, 350, 500, 1000, 1500, 10E5, 10E6, 10E7 Å) and <strong>several particle sizes</strong> (5, 7, 10, 12, 20 µm) as a choice to customize for every application task.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Size_Exclusion_principle_2a432edcc1.webp" alt="KNAUER: Size Exclusion - principle" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Size_Exclusion_principle_2a432edcc1.webp 228w," sizes="100vw" width="1024" height="700"></p><h3><strong>KNAUER AppliChrom® ABOA columns for aqueous SEC, organic GPC, and special applications</strong></h3><ul><li>AppliChrom® ABOA StyDiViBe Series GPC-columns for GPC analysis of organic molecules using THF, toluene, chloroform.</li><li>AppliChrom ABOA SuperOH-P Series for aqueous GPC/SEC-analysis of mainly neutral and anionic polymers like heparins, polysaccharides, PEO,<br>PEG,....</li><li>AppliChrom® ABOA DMSO-Phil-P Series for DMSO GPC/SEC analysis (humic substances, UF/MUF-glue, p-NIPA, many polycondensates, protein from pea or leguminosae,...)</li><li>AppliChrom® ABOA DMAc-Phil-P Series for GPC/ SEC analysis in DMAc, DMF, NMP. Good for many medium polar substances, polyoxazolines, lignin,...</li><li>AppliChrom® ABOA Cat-Phil-P Series for aqueous GPC/SEC-analysis of special aminic and polycationic polymers like p-DADMAc, PEI/ polyethylenimin, chitosan, copolymers with polyaminofunctions, but also useful for polyanions or neutral polymers like heparin or PEO/PEG, dextran or pullulan.</li><li><strong>Seperation Ranges: </strong>100–50 000 000 Da</li></ul><h4><strong>You decide between classical GPC/SEC columns with conventional mixed pore technology or AppliChrom® BPT technology:</strong></h4><p>Separate oligomers and polymers with the new GPC media line from AppliChrom® BPT synthesis technology for large range, high resolution separations with increased accuracy in calibration (patent pending).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg" alt="KNAUER: AppliChrom® ABOA columns." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Appli_Chrom_ABOA_columns_58d5d380f6.jpg 245w," sizes="100vw" width="1379" height="718"></p>

KNAUER Sepapure® FPLC Columns for Antibody Affinity Chromatography

 The FPLC media is functionalized either with Protein A or Protein G ligands for the binding of antibodies or antibody fragments.

<p><strong>FPLC media</strong> based on cross-linked agarose beads with a mean diameter of 100 µm. &nbsp;The FPLC media is functionalized either with <strong>Protein A</strong> or<strong> Protein G ligands</strong> for the binding of antibodies or antibody fragments.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Antibody_Affinity_Protein_A_and_G_08afa2179e.webp" alt="KNAUER: FPLC Column for Antibody Affinity - Protein A and G" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Antibody_Affinity_Protein_A_and_G_08afa2179e.webp 236w," sizes="100vw" width="690" height="456"></p><h4><strong>Properties:</strong></h4><p>Sepapure® Affinity media for antibodies or antibody fragments is designed to be used with most aqueous buffer systems. It is long lasting when correctly handled and are compatible with common CIP strategies. All Sepapure® media is stored in 20 % ethanol upon delivery. Available as prepacked 1 ml or 5 ml cartridge or as bulk material.</p><h4><strong>Technical data:</strong></h4><p>Agarose beads with typical loading ranges of 1 - 30 mg/l (Protein A column) or 1 - 15 mg/ml (Protein G column). The maximum operating pressure the<br>Sepapure® columns should be used at is 3 bar, while the recommended flowrate is 1 CV/ml.</p><h4><strong>Recommended application areas:</strong></h4><p>Typically used in the first step („capture“) of an FPLC purification procedure.</p><h3><strong>KNAUER Sepapure®&nbsp;</strong></h3><p>In protein purification, a combination of different methods is needed for a successful separation. The purity of the wanted biomolecule is increased in three steps: <strong>Capture - Intermediate - Polishing</strong></p><p>In the “<strong>capture step</strong>” the crude biomolecule is extracted from major side products. In the “i<strong>ntermediate step</strong>” further contaminations are removed, and the highly pure biomolecule is gained in the “<strong>polishing step</strong>”. For each step a different method and therefore different columns are used.&nbsp;</p><p><strong>FPLC Sepapure® columns</strong> are dedicated for <strong>purification of biomolecules</strong>. Sepapure® <strong>media for affinity chromatography (AC) </strong>and<strong> Ion-Exchange chromatography (IEX)</strong> is based on Agarose and depending on the specific purification mode functionalized with ligands e.g. Protein A, Ni-NTA or quaternary ammonium (Q). These FPLC media are available packed in 1 ml and 5 ml cartridges or as bulk media.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_FPLC_Sepapure_columns_ad980345f3.jpg" alt="KNAUER: FPLC Sepapure® columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_FPLC_Sepapure_columns_ad980345f3.jpg 245w," sizes="100vw" width="1372" height="562"></p><h5><strong>Physical properties FPLC Sepapure® columns</strong></h5><ul><li>Resin 100 µm Agarose particles with 4 % or 6 % crosslinking</li><li><strong>Flowrate Recommended: </strong>1 CV/ml</li><li><strong>pH Stability</strong> 3 – 9 long term</li><li><strong>Max. pressure</strong> 3 bar</li></ul><p>Sepapure® Desalting columns are based on Dextran with an exclusion limit of 5 kDa and available in 1 ml and 5 ml cartridges.&nbsp;</p><h5><strong>Physical properties Sepapure® Desalting columns</strong></h5><ul><li>Resin 20 – 50 µm Dextran particles</li><li><strong>Flowrate Recommended:</strong> 1 CV/ml</li><li><strong>Exclusion limit</strong> 5 kDa</li><li><strong>Max. pressure</strong> 3 bar</li></ul>

KNAUER Sepapure® FPLC Columns for Tag-Affinity Chromatography

FPLC media based on cross-linked agarose beads with a mean diameter of 100 µm.  The FPLC media is functionalized with NTA ligands for His-Tag.

<p><strong>FPLC media</strong> based on cross-linked agarose beads with a mean diameter of 100 µm. &nbsp;The FPLC media is functionalized with NTA ligands for His-Tag.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Antibody_Affinity_Protein_A_and_G_08afa2179e.webp" alt="KNAUER: FPLC Column for Tag-Affinity Chromatography" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Antibody_Affinity_Protein_A_and_G_08afa2179e.webp 236w," sizes="100vw" width="690" height="456"></p><h4><strong>Properties:</strong></h4><p>Sepapure® Affinity media for recombinantly tagged proteins is designed to be used with most aqueous buffer systems. It is long lasting when correctly handled and compatible with common CIP strategies. All Sepapure® media is stored in 20 % ethanol upon delivery. Available as prepacked 1 ml or 5 ml cartridge or as bulk material.</p><h4><strong>Technical data:</strong></h4><p>Agarose beads with typical loading ranges of 1–40 mg/l (Ni-NTA). The maximum operating pressure the Sepapure® columns should be used at is 3 bar, while the recommended flowrate is 1 CV/ml. &nbsp;Available as prepacked 1 ml or 5 ml cartridge or as bulk material.</p><h4><strong>Recommended application areas:</strong></h4><p>Typically used in the first step („capture“) of an FPLC purification procedure.</p><h3><strong>KNAUER Sepapure®&nbsp;</strong></h3><p>In protein purification, a combination of different methods is needed for a successful separation. The purity of the wanted biomolecule is increased in three steps: <strong>Capture - Intermediate - Polishing</strong></p><p>In the “<strong>capture step</strong>” the crude biomolecule is extracted from major side products. In the “i<strong>ntermediate step</strong>” further contaminations are removed, and the highly pure biomolecule is gained in the “<strong>polishing step</strong>”. For each step a different method and therefore different columns are used.&nbsp;</p><p><strong>FPLC Sepapure® columns</strong> are dedicated for <strong>purification of biomolecules</strong>. Sepapure® <strong>media for affinity chromatography (AC) </strong>and<strong> Ion-Exchange chromatography (IEX)</strong> is based on Agarose and depending on the specific purification mode functionalized with ligands e.g. Protein A, Ni-NTA or quaternary ammonium (Q). These FPLC media are available packed in 1 ml and 5 ml cartridges or as bulk media.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_FPLC_Sepapure_columns_ad980345f3.jpg" alt="KNAUER: FPLC Sepapure® columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_FPLC_Sepapure_columns_ad980345f3.jpg 245w," sizes="100vw" width="1372" height="562"></p><h5><strong>Physical properties FPLC Sepapure® columns</strong></h5><ul><li>Resin 100 µm Agarose particles with 4 % or 6 % crosslinking</li><li><strong>Flowrate Recommended: </strong>1 CV/ml</li><li><strong>pH Stability</strong> 3 – 9 long term</li><li><strong>Max. pressure</strong> 3 bar</li></ul><p>Sepapure® Desalting columns are based on Dextran with an exclusion limit of 5 kDa and available in 1 ml and 5 ml cartridges.&nbsp;</p><h5><strong>Physical properties Sepapure® Desalting columns</strong></h5><ul><li>Resin 20 – 50 µm Dextran particles</li><li><strong>Flowrate Recommended:</strong> 1 CV/ml</li><li><strong>Exclusion limit</strong> 5 kDa</li><li><strong>Max. pressure</strong> 3 bar</li></ul>

KNAUER Sepapure® FPLC Columns for Ion-Exchange Chromatography

The FPLC media is functionalized with different linkers ranging from strong anion exchange ligands to weak cation exchange ligands. 

<p>FPLC media based on cross-linked agarose beads with a mean diameter of 100 µm. &nbsp;The FPLC media is functionalized with different linkers ranging from strong anion exchange ligands to weak cation exchange ligands.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Ion_Exchange_Chromatography_640e6f5ba7.jpg" alt="KNAUER: Sepapure FPLC Columns for Ion Exchange Chromatography" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Ion_Exchange_Chromatography_640e6f5ba7.jpg 236w," sizes="100vw" width="1920" height="1270"></p><h4><strong>Properties:</strong></h4><p>Sepapure® Ion-Exchange media is designed to be used with most aqueous buffer systems. It is long lasting when correctly handled and is compatible with common CIP strategies. All Sepapure® media is stored in 20 % ethanol upon delivery. &nbsp;</p><h4><strong>Technical data:</strong></h4><p>The agarose beads are functionalized with a quaternary ammonium for the strong anion exchanger (Q) and with diethylaminoethyl for the weak anion exchanger (DEAE). The strong cation exchanger is functionalized with sulphopropyl (SP) and the weak cation exchanger with carboxymethyl (CM).&nbsp;</p><p>The maximum operating pressure the Sepapure® columns should be used at is 3 bar, while the recommended flowrate is 1 CV/ml.&nbsp;</p><p>Available as prepacked 1 ml or 5 ml cartridge or as bulk material.&nbsp;</p><h4><strong>Recommended application areas:</strong></h4><p>Typically used in the intermediate step of an FPLC purification procedure.</p><h3><strong>KNAUER Sepapure®&nbsp;</strong></h3><p>In protein purification, a combination of different methods is needed for a successful separation. The purity of the wanted biomolecule is increased in three steps: <strong>Capture - Intermediate - Polishing</strong></p><p>In the “<strong>capture step</strong>” the crude biomolecule is extracted from major side products. In the “i<strong>ntermediate step</strong>” further contaminations are removed, and the highly pure biomolecule is gained in the “<strong>polishing step</strong>”. For each step a different method and therefore different columns are used.&nbsp;</p><p><strong>FPLC Sepapure® columns</strong> are dedicated for <strong>purification of biomolecules</strong>. Sepapure® <strong>media for affinity chromatography (AC) </strong>and<strong> Ion-Exchange chromatography (IEX)</strong> is based on Agarose and depending on the specific purification mode functionalized with ligands e.g. Protein A, Ni-NTA or quaternary ammonium (Q). These FPLC media are available packed in 1 ml and 5 ml cartridges or as bulk media.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_FPLC_Sepapure_columns_ad980345f3.jpg" alt="KNAUER: FPLC Sepapure® columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_FPLC_Sepapure_columns_ad980345f3.jpg 245w," sizes="100vw" width="1372" height="562"></p><h5><strong>Physical properties FPLC Sepapure® columns</strong></h5><ul><li>Resin 100 µm Agarose particles with 4 % or 6 % crosslinking</li><li><strong>Flowrate Recommended: </strong>1 CV/ml</li><li><strong>pH Stability</strong> 3 – 9 long term</li><li><strong>Max. pressure</strong> 3 bar</li></ul><p>Sepapure® Desalting columns are based on Dextran with an exclusion limit of 5 kDa and available in 1 ml and 5 ml cartridges.&nbsp;</p><h5><strong>Physical properties Sepapure® Desalting columns</strong></h5><ul><li>Resin 20 – 50 µm Dextran particles</li><li><strong>Flowrate Recommended:</strong> 1 CV/ml</li><li><strong>Exclusion limit</strong> 5 kDa</li><li><strong>Max. pressure</strong> 3 bar</li></ul>

KNAUER Sepapure® FPLC Desalting Columns

Desalting columns based on dextran for group separations of large molecular weight biomolecules from small molecular weight compounds and salts.

<p>FPLC media based on dextran beads with a mean diameter of 20–50 µm. Size exclusion chromatography (SEC) separates molecules on the basis of size differences. KNAUER offers <strong>desalting columns</strong> based on <strong>dextran</strong> for <strong>group separations</strong> of large molecular weight <strong>biomolecules</strong> <strong>from small molecular</strong> weight compounds and <strong>salts.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Size_Exclusion_chromatography_fb9d43e34b.jpg" alt="KNAUER: Sepapure FPLC - Desalting or Buffer Exchange" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Size_Exclusion_chromatography_fb9d43e34b.jpg 156w," sizes="100vw" width="1500" height="1500"></p><h4><strong>Properties:</strong></h4><p>Sepapure® Desalting media is designed to be used for removal of small molecules below the exclusion limit and for covenient rebuffering. It is long lasting when correctly handled and compatible with common CIP strategies. All Sepapure® media is stored in 20 % ethanol upon delivery. Available as prepacked 5 ml cartridge or as bulk material.&nbsp;</p><h4><strong>Technical data:</strong></h4><p>The Sepapure® dextran beads have an exclusion limit of 5 kDa. The maximum operating pressure of the Sepapure® columns is 3 bar, while the recommended flowrate is 1 CV/ml.</p><h4><strong>Recommended application areas:</strong></h4><p>Typically used in the final step of an FPLC purification procedure or inbetween steps for fast buffer exchange.</p><h3><strong>KNAUER Sepapure®&nbsp;</strong></h3><p>In protein purification, a combination of different methods is needed for a successful separation. The purity of the wanted biomolecule is increased in three steps: <strong>Capture - Intermediate - Polishing</strong></p><p>In the “<strong>capture step</strong>” the crude biomolecule is extracted from major side products. In the “i<strong>ntermediate step</strong>” further contaminations are removed, and the highly pure biomolecule is gained in the “<strong>polishing step</strong>”. For each step a different method and therefore different columns are used.&nbsp;</p><p><strong>FPLC Sepapure® columns</strong> are dedicated for <strong>purification of biomolecules</strong>. Sepapure® <strong>media for affinity chromatography (AC) </strong>and<strong> Ion-Exchange chromatography (IEX)</strong> is based on Agarose and depending on the specific purification mode functionalized with ligands e.g. Protein A, Ni-NTA or quaternary ammonium (Q). These FPLC media are available packed in 1 ml and 5 ml cartridges or as bulk media.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_FPLC_Sepapure_columns_ad980345f3.jpg" alt="KNAUER: FPLC Sepapure® columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_FPLC_Sepapure_columns_ad980345f3.jpg 245w," sizes="100vw" width="1372" height="562"></p><h5><strong>Physical properties FPLC Sepapure® columns</strong></h5><ul><li>Resin 100 µm Agarose particles with 4 % or 6 % crosslinking</li><li><strong>Flowrate Recommended: </strong>1 CV/ml</li><li><strong>pH Stability</strong> 3 – 9 long term</li><li><strong>Max. pressure</strong> 3 bar</li></ul><p>Sepapure® Desalting columns are based on Dextran with an exclusion limit of 5 kDa and available in 1 ml and 5 ml cartridges.&nbsp;</p><h5><strong>Physical properties Sepapure® Desalting columns</strong></h5><ul><li>Resin 20 – 50 µm Dextran particles</li><li><strong>Flowrate Recommended:</strong> 1 CV/ml</li><li><strong>Exclusion limit</strong> 5 kDa</li><li><strong>Max. pressure</strong> 3 bar</li></ul>

Products focused on LC columns distributed by KNAUER - page 4

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