Classical and hydrophilic/aqueous C8 columns for HPLC and UHPLC (USP L7)
C8 is a silica gel based stationary phase in reversed phase mode with alternative selectivity compared to C18 phases. Compared to C18 phases, these columns show less retention due to the lower hydrophobicity.
- Ultra pure, spherical high performance HPLC phase based on silica gel
- Monomeric C8 (Octyl) modification, standard endcapping, with 10 % carbon content (~ 50 % endcapping)
Properties
- Separation mechanism: Hydrophobic interaction(lower compared to C18 phases)
Key features
- Classical C8 phase, standard 50 % endcapping and 10 % carbon load, outstanding mechanical and chemical stability, suited for analytical as well as semi preparative and preparative applications.
Recommended application areas
- Similar selectivity to C18 phase but less retention due to the lower hydrophobicity; useful for determination of:
- Water soluble vitamins
- Steroids
- Catecholamines
- Sedatives
KNAUER: C8 RP Non-endcapped
Hydrophilic C8A phase offers an alternative, more polar selectivity. It is suited for highly polar reversed phase applications with up to 100 % aqueous mobile phases.
KNAUER: C8A
Tip: Caution! Even for short term storage all buffer solutions have to be rinsed from the column to prevent crystallization effects.
Stationary phases Eurospher II 100 C8 and C8A are provided in these column types: analytical, semi-preparative and preparative.
Eurospher II 100 C8 and C8A Analytical Columns are available with these parameters:
- Column length (mm): 50, 100, 150, 250
- Column ID (mm): 2, 3, 4, 4.6
- Particle size (µm): 2, 3, 5
Eurospher II 100 C8 and C8A Semi-Preparative Standard Columns are available with these parameters:
- Column length (mm): 30, 50, 125, 150, 250
- Column ID (mm): 8, 16
- Particle size (µm): 5, 10
KNAUER (U)HPLC Columns
Widest range of HPLC and UHPLC columns for all separation modes and applications.
- Safe Investment: Robust and reliable columns for attractive price
- Separation Mode: Reversed Phase, HILIC, Normale
- Simplicity: Easy purchase via our online store with fast delivery
- Application: All-rounder for research or highly specialised for high-throughput analysis? We have the best column for your requirements.
- Flexibility: Wide selection of column and particle sizes, for (U)HPLC applications up to 1000 bar
KNAUER offers the perfect column for any small molecule separation. No matter which separation mode, molecule and the mobile phase, we offer a suitable (U)HPLC stationary phase. Choose from a wide range of modifications, particle sizes and column dimensions to suit your application. To elongate the lifetime of your column, you can choose to use a suitable precolumn.
KNAUER: KNAUER (U)HPLC Columns
