<p><span><strong>Commercial and public spectral libraries provide broad coverage for LC-MS/MS identification, but they cannot contain every compound that matters to a specific laboratory. Proprietary products, metabolites, intermediates, impurities, degradation products, biomarkers, and internally characterized compounds may be highly relevant even if they are absent from standard reference collections. NIST26 allows laboratories to build their own MS/MS user libraries and integrate them directly into routine search workflows, creating a focused resource tailored to their analytical needs.</strong></span></p><h4><span><strong>Why Create a User MS/MS Library?</strong></span></h4><p><span>User libraries are usually much smaller than commercial or public spectral databases, but their value is not determined by size alone.</span></p><p><span>A laboratory-specific library may contain exactly the compounds most important for a particular application. These can include:</span></p><ul><li><span>proprietary products,</span></li><li><span>known metabolites,</span></li><li><span>synthesis intermediates,</span></li><li><span>impurities,</span></li><li><span>degradation products,</span></li><li><span>biomarkers,</span></li><li><span>compounds associated with specific industrial or environmental samples.</span></li></ul><p><span>Such libraries can therefore provide highly relevant identifications in pharmaceutical development, industrial R&amp;D, medical laboratories, environmental analysis, and other specialized fields. A relatively small collection of carefully characterized compounds may be more useful for a particular laboratory than a much larger general-purpose database.&nbsp;</span></p><h4><span><strong>What Information Can Be Stored with Each Spectrum?</strong></span></h4><p><span>Creating a useful library entry involves more than importing an MS/MS spectrum.</span></p><p><span>A complete record can combine several types of information, including the experimental spectrum, chemical structure, instrumental parameters, ionization information, sample history, and additional user-defined metadata.</span></p><p><span>Spectra can be transferred from vendor software into NIST Library Search, or they can be sent from the Chromatogram tab directly to the Library Search environment in NIST26. Chemical structures can be imported through the Windows clipboard from a molecular drawing program, while standard experimental parameters and sample-history information can be prepared separately and added to the entry.&nbsp;</span></p><p><span>The ability to keep analytical metadata together with the spectrum is particularly important for internally generated libraries because it preserves the context in which the reference spectrum was acquired.</span></p><h4><span><strong>Using Tags to Organize LC-MS/MS Metadata</strong></span></h4><p><span>NIST26 supports a range of structured tags for LC-MS/MS spectra.</span></p><p><span>These can describe parameters such as:</span></p><ul><li><span>spectrum type,</span></li><li><span>compound type,</span></li><li><span>ion name,</span></li><li><span>precursor type,</span></li><li><span>precursor </span><i><span>m/z</span></i><span>,</span></li><li><span>collision energy,</span></li><li><span>instrument type,</span></li><li><span>sample identifier,</span></li><li><span>ionization mode,</span></li><li><span>ion polarity,</span></li><li><span>collision gas,</span></li><li><span>pressure,</span></li><li><span>mass range,</span></li><li><span>maximum intensity,</span></li><li><span>cone voltage,</span></li><li><span>charge,</span></li><li><span>molecular weight,</span></li><li><span>known impurities,</span></li><li><span>related CAS numbers,</span></li><li><span>peptide sequence and peptide modifications.</span></li></ul><p><span>This provides a convenient way to attach searchable and standardized experimental information to spectra rather than leaving all metadata in an unstructured comment field.&nbsp;</span></p><p><span>Tags can be entered individually through the <strong>Additional Info</strong> section in the Librarian interface, but when many spectra are being added, a grouped approach can be considerably faster.&nbsp;</span></p><h4><span><strong>Adding Groups of Tags Efficiently</strong></span></h4><p><span>For repetitive workflows, metadata can be prepared in advance and pasted into NIST26 as a group.</span></p><p><span>The presentation recommends maintaining frequently used tag sets in a simple text document, then copying them into the synonym field and using <strong>Add to List</strong> to convert them into structured information.</span></p><p><span>This is especially useful when the vendor software already transfers some parameters automatically. Users can then add only the missing information, such as ion adduct or polarity, rather than duplicating metadata already imported with the spectrum.&nbsp;</span></p><p><span>Care is required because entering information that is already present may create duplicate metadata.</span></p><p><span>This workflow can substantially accelerate library creation when many spectra share the same instrument configuration or acquisition parameters.</span></p><h4><span><strong>Preserving Sample History</strong></span></h4><p><span>A spectral match is more useful when the origin of the reference spectrum is known.</span></p><p><span>NIST26 therefore allows detailed sample information to be stored in the comment field. For batches of related samples, the text can be prepared in Notepad or another editor and pasted into multiple records.</span></p><p><span>Examples of useful information include sampling location, sampling date and time, analyst name, organization, internal sample identifier, project information, or other relevant context.&nbsp;</span></p><p><span>For environmental, clinical, pharmaceutical, and industrial laboratories, this information can become an important part of data traceability.</span></p><h4><span><strong>Adding Molecular Structures</strong></span></h4><p><span>A user library becomes considerably more useful when spectra are linked to molecular structures.</span></p><p><span>Structures can be created from scratch in a chemical drawing program. Alternatively, a similar structure can be located in NIST Search and sent to the default structure editor, where it can be modified as needed.</span></p><p><span>Once completed, the structure can be copied to the Windows clipboard and inserted into the NIST Librarian spectrum-information window. NIST26 can then calculate the molecular formula and molecular weight from the imported structure.&nbsp;</span></p><p><span>This linkage between spectrum and structure supports more informative library displays and structure-based searching.</span></p><h4><span><strong>Creating Custom User-Defined Tags</strong></span></h4><p><span>In addition to the standard LC-MS/MS tags, laboratories can define their own metadata fields.</span></p><p><span>Examples shown include identifiers such as:</span></p><ul><li><span>chemist,</span></li><li><span>laboratory notebook number,</span></li><li><span>analyst.</span></li></ul><p><span>The information can be prepared in a text editor and added using the appropriate tag syntax. Once configured in the <strong>Comment Field Display</strong> settings, these values are displayed as separate structured lines rather than remaining embedded in the general comments.&nbsp;</span></p><p><span>This flexibility makes the library more suitable for laboratories with established internal documentation systems or quality-management requirements.</span></p><p><span>NIST can also automatically add information such as the InChIKey and an estimated retention index when appropriate structural information is available.&nbsp;</span></p><h4><span><strong>High-Resolution and Low-Resolution Libraries Should Be Kept Separate</strong></span></h4><p><span>User libraries do not have to contain only high-resolution spectra.</span></p><p><span>Low-resolution MS/MS spectra may also be valuable, including spectra generated by:</span></p><ul><li><span>in-source collision on single-quadrupole instruments,</span></li><li><span>triple-quadrupole MS/MS,</span></li><li><span>other nominal-mass workflows.</span></li></ul><p><span>The recommendation is to maintain <strong>separate high-resolution and low-resolution user libraries</strong>, because the appropriate search strategies and handling can differ.&nbsp;</span></p><p><span>Low-resolution spectra can still generate strong library-search results and may be particularly useful when laboratories want to preserve historical data or incorporate spectra from multiple instrument types.</span></p><h4><span><strong>Important Software Considerations When Importing Spectra</strong></span></h4><p><span>The presentation also highlights several practical software issues that users should be aware of.</span></p><p><span>One concerns the <strong>Intensity Threshold</strong> setting in the Spectrum Import Options window. When an intensity threshold is applied during import from vendor software, high-resolution spectral values may be converted to nominal mass rather than preserving the expected decimal precision.</span></p><p><span>For laboratories building high-resolution user libraries, this is an important detail because accurate-mass information is one of the main advantages of HRMS data.</span></p><p><span>Another issue affects some library-search modes after high-resolution spectra are added to a user library.</span></p><h4><span><strong>HiRes No Precursor and Hybrid Search Limitations</strong></span></h4><p><span>After adding high-resolution spectra, certain <strong>HiRes NoPrecursor</strong> and <strong>Similarity Hybrid MS/MS</strong> searches may not function correctly.</span></p><p><span>A workaround involves exporting the affected library using <strong>Lib2NIST</strong> into MSP ASCII files together with the associated molecular structure files, then importing the data back into NIST library format.&nbsp;</span></p><p><span>The presentation specifically warns against attempting the problematic MS/MS Hybrid Search under these conditions because it may cause the software to crash.</span></p><p><span>An EI Normal search can mimic some of the functionality of HiRes No Precursor and may work effectively when high- and low-resolution libraries are searched together. HiRes NoPrecursor can also operate with presearch disabled, although this can make searching considerably slower.&nbsp;</span></p><h4><span><strong>Display Issues in the Chromatogram Window</strong></span></h4><p><span>Another software behavior concerns how spectral values are displayed in the Chromatogram tab.</span></p><p><span>Values from Spectrum Import Options are reflected in the head-to-head spectral display. If settings are adjusted for low-resolution spectra, the displayed masses may appear truncated.</span></p><p><span>Importantly, this visual behavior does <strong>not</strong> affect the results in the accurate-mass deconvolution and library-searching table; it affects only the spectral display at the top of the Chromatogram window.&nbsp;</span></p><p><span>Distinguishing display behavior from actual processing results is important to avoid misinterpreting valid search results as corrupted data.</span></p><h4><span><strong>Updating the Library After Adding New Entries</strong></span></h4><p><span>User-library maintenance does not end when a spectrum has been added.</span></p><p><span>Whenever a new entry containing a molecular structure is introduced, the <strong>structure index should be updated</strong> so that the new compound can be included properly in structure searching.</span></p><p><span>The InChIKey appears to update with the structure, but if duplicate records are not being handled correctly, the InChIKey index may also need to be rebuilt.&nbsp;</span></p><p><span>If a new library folder is added to the NIST26 MSSEARCH directory while the software is already running, users should also update the library list so that the newly added collection becomes available without restarting the entire environment.&nbsp;</span></p><h4><span><strong>Using Lib2NIST to Repair High-Resolution User Libraries</strong></span></h4><p><span>The <strong>Lib2NIST</strong> utility plays an important role in resolving some high-resolution library-search issues.</span></p><p><span>The workflow involves exporting the problematic high-resolution library to an MSP file together with its molecular structure files and then re-importing the data into NIST library format.</span></p><p><span>The corrected library can then be placed back into the NIST26 MSSEARCH directory. It is advisable to preserve the original library under a different name until the repaired version has been tested successfully.&nbsp;</span></p><p><span>The presentation also recommends closing NIST Search while performing Lib2NIST conversions.</span></p><h4><span><strong>Small Libraries Can Deliver High Analytical Value</strong></span></h4><p><span>The central message is straightforward: a user library does not need to contain hundreds of thousands of compounds to be useful.</span></p><p><span>A carefully curated collection of spectra from compounds that repeatedly appear in a laboratory's own samples can dramatically improve identification relevance. This is especially valuable for compounds that are proprietary, newly synthesized, application-specific, or otherwise poorly represented in public and commercial databases.</span></p><p><span>By combining MS/MS spectra with structures, acquisition metadata, sample history, tags, and internally defined information, NIST26 can become more than a search engine for commercial databases. It can serve as a laboratory-specific knowledge base built around compounds that genuinely matter to the user's work.</span></p><p><span>For specialized LC-MS/MS workflows, that may be one of the most valuable ways to extend the capabilities of spectral library searching.</span></p>

Creating MS/MS User Libraries in NIST26

<p><strong>Wiley announces the expansion of its spectral libraries across IR and Raman, adding empirical and computed reference data spanning forensics, pigments and dyes, mineralogy, materials, polymers, and pharmaceuticals. This release adds over a thousand new empirical reference spectra, plus more than 429,000 new SmartSpectra records, across dozens of updated and new databases. This brings the combined collections to over 1.4 million IR spectra and 230,000 Raman spectra.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Wiley_Wiley_continues_to_expand_its_IR_Raman_and_Smart_Spectra_spectral_libraries_806b34cdc1.jpg" alt="Wiley: Wiley continues to expand its IR, Raman, and SmartSpectra spectral libraries" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Wiley_Wiley_continues_to_expand_its_IR_Raman_and_Smart_Spectra_spectral_libraries_806b34cdc1.jpg 245w," sizes="100vw" width="1600" height="838"></p><p>“<i>Scientific discoveries and business decisions depend on trustworthy analytical results,</i>” said <strong>Graeme Whitley</strong>, senior director, data science solutions at Wiley. “<i>The value of a spectral library expansion is not simply that it adds more records. It gives analysts more relevant reference candidates when they encounter unfamiliar materials, whether they are verifying a polymer, investigating a pharmaceutical sample, examining a mineral, or screening a forensic specimen.</i>”</p><h4><strong>Highlights include:</strong></h4><ul><li><strong>Forensics:</strong> A new Raman forensics database adds reference spectra for colorant and dye identification on textiles, drawn from real fiber-evidence research, useful for investigators working on dye and textile analysis.</li><li><strong>Pigments and dyes:</strong> New Raman and IR data significantly broadens pigment and dye coverage, including historic artists' pigments, supporting art conservation and cultural-heritage authentication work.</li><li><strong>Minerals: </strong>New Raman and IR data extends coverage to minerals from different localities and Martian mineral analogs. This release includes new Raman additions from our ongoing collaboration with the American Museum of Natural History, now cross-referenced with DANA Classification Numbers and Mindat IDs, giving mineralogists and gemologists an easier way to identify entries against established references.</li><li><strong>Materials and semiconductors: </strong>New Raman and IR data covers crystal growth relevant to battery materials, rare-earth-doped magnetic crystals, and flexible and 2D semiconductor materials, useful for researchers in battery, magnetic materials, and electronics work.</li><li><strong>Polymers: </strong>New Raman and IR data spans 3D-printed, MOF-integrated microlattices, environmental plastic aging and recycled PET materials, and food packaging plastics. New Raman reference spectra also cover more than two dozen synthetic polymer types for identifying microplastics in environmental samples.</li><li><strong>Clinical and pharma:</strong> New Raman and IR data covers antibiotic classes, including beta-lactams, and the WHO's essential antibiotic list, plus bone mineralization studies in clinical models. A new IR dataset also adds spectra for common over-the-counter medications, including aspirin, ibuprofen, and paracetamol, helping quality control teams confirm formulations faster.</li><li><strong>SmartSpectra: </strong>Our SmartSpectra IR, Raman, and vapor-phase IR collections continue to grow, adding<strong> 243,000+ IR</strong>, <strong>67,000+ Raman</strong>, and <strong>119,000+ vapor-phase IR</strong> computed reference spectra to support broad, high-throughput identification.</li></ul><blockquote><p class="cke-user_style-important"><a target="_blank" rel="noopener noreferrer" href="https://sciencesolutions.wiley.com/spectral-databases/"><strong>Learn more about Wiley Spectral Databases</strong></a></p><p class="cke-user_style-important"><a target="_blank" rel="noopener noreferrer" href="https://sciencesolutions.wiley.com/how-to-update-knowitall-databases/"><strong>Existing subscribers can find guidance on updating their databases here</strong></a></p></blockquote><h4><strong>Wiley Spectral Databases</strong></h4><p>Wiley's spectral databases are consistently evolving to meet today's research demands, covering multiple spectral techniques across a wide range of applications including polymers, materials, environmental, forensics, toxicology, pharmaceutical, biotech, automotive, aerospace, food, cosmetics, and more. Built on rigorous quality protocols, Wiley delivers industry-leading spectral data and intelligent tools to accelerate analysis and deliver deeper insights — making it the authoritative, trusted source that analytical labs worldwide rely on.&nbsp;</p>

Wiley continues to expand its IR, Raman, and SmartSpectra spectral libraries

<p><strong>Per- and polyfluoroalkyl substances (PFAS)&nbsp;analysis is no longer&nbsp;defined by&nbsp;a fixed list of known compounds.</strong></p><p>As regulatory frameworks evolve and scientific understanding deepens, laboratories are being asked to go beyond routine testing. In addition to quantifying known PFAS, they must also investigate unknown, unexpected, and emerging compounds that fall outside predefined target lists.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_509ce49fc2.webp" alt="Thermo Fisher Scientific: PFAS Analysis Beyond the Target List: Connecting Quantitation and Discovery in a Single Workflow" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_509ce49fc2.webp 245w," sizes="100vw" width="1024" height="526"></p><p>This shift introduces a fundamental analytical challenge.</p><p>Targeted quantitation provides robust and validated concentration data for known PFAS. However, by design, it only answers questions about compounds already included in the method. In contrast, non-targeted analysis,&nbsp;using high-resolution accurate-mass&nbsp;(HRAM)&nbsp;data,&nbsp;enables scientists to explore the broader chemical landscape, revealing&nbsp;additional&nbsp;PFAS&nbsp;that were not included in a targeted quantitation method.</p><p>For PFAS, where thousands of structurally related compounds and transformation products may be present, neither approach&nbsp;alone provides a complete picture.</p><p>The&nbsp;real challenge&nbsp;lies in connecting these workflows. Discovery findings must be translated into actionable results that can be reviewed, followed up, and reported within a controlled analytical framework.</p><p><i>In a previous<strong>&nbsp;</strong></i><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/article/7167"><i><strong>blog post</strong></i></a><i>, we introduced how </i><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1759"><i><strong>Thermo Scientific Compound Discoverer software </strong></i></a><i>and </i><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/76"><i><strong>Thermo Scientific Chromeleon Chromatography Data System&nbsp;(CDS)</strong>&nbsp;</i></a><i>connect discovery and routine workflows.&nbsp;Here, we explore what that connection looks like in practice using PFAS analysis as an example.</i></p><h3><strong>Why PFAS&nbsp;requires&nbsp;more than one analytical question</strong></h3><p>PFAS&nbsp;represent a&nbsp;large&nbsp;and diverse class of compounds,&nbsp;with&nbsp;some&nbsp;estimates exceeding<strong>&nbsp;40,000 individual substances</strong>. Regulatory methods typically focus on a limited subset, yet environmental and biological samples often&nbsp;contain&nbsp;many more&nbsp;PFAS, including precursors and transformation products.</p><p><strong>This creates two&nbsp;essential and&nbsp;complementary analytical questions:</strong></p><p style="text-align:center;"><i>How much of these known PFAS are present?</i></p><p style="text-align:center;">and</p><p style="text-align:center;"><i>What other PFAS-related compounds might be present?</i></p><p>A complete workflow needs to support both&nbsp;questions.</p><h3><strong>Starting with targeted quantitation</strong></h3><p>For known PFAS, targeted quantitation&nbsp;remains&nbsp;the&nbsp;backbone of routine analysis.</p><p>Chromeleon CDS provides&nbsp;a&nbsp;controlled environment for&nbsp;data acquisition, processing, calibration, and reporting. In a PFAS workflow,&nbsp;this supports routine quantitation of predefined targets using laboratory-specific method and review criteria.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_2_7ff8eda8fe.jpg" alt="Thermo Fisher Scientific: PFAS Analysis Beyond the Target List: Connecting Quantitation and Discovery in a Single Workflow: Figure 1. Targeted quantitation of known PFAS in Chromeleon CDS.&nbsp;This example is&nbsp;from the analysis of PFAS using a&nbsp;Thermo Scientific&nbsp;Orbitrap Exploris&nbsp;240. Here&nbsp;we are showing&nbsp;the&nbsp;calibration curve (right) and extracted&nbsp;ion chromatogram&nbsp;(left)&nbsp;of 7:3 FTCA&nbsp;at 6 ng/L.&nbsp;The bottom&nbsp;half&nbsp;shows the processing method that&nbsp;contains&nbsp;many target PFAS and&nbsp;isotopically-labelled&nbsp;internal standards." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_2_7ff8eda8fe.jpg 208w," sizes="100vw" width="794" height="596"></p><p>This targeted workflow is essential, but it is only part of the story. A sample may contain PFAS-related compounds not included in the original method.</p><h3><strong>Looking beyond known PFAS</strong></h3><p>Once HRAM data have been acquired, the same dataset can be explored beyond the target list using Compound Discoverer software. This enables comprehensive analysis of complex datasets, including:</p><ul><li>Detection and alignment of unknown features</li><li>Statistical comparison across samples and blanks</li><li>Spectral library searching and database annotation</li><li>Evaluation of isotope patterns, fragmentation, and homologous series</li></ul><p>For PFAS, this is particularly valuable. The software can leverage characteristic properties such as negative mass defect, CF₂-based homologous series, and diagnostic fragment ions to prioritize PFAS-related candidates and assign confidence to putative identifications.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_3_e523e1cdc9.jpg" alt="Thermo Fisher Scientific: PFAS Analysis Beyond the Target List: Connecting Quantitation and Discovery in a Single Workflow: Figure 2. Discovery of additional PFAS-related components in Compound Discoverer software. Here we show an example of using PFAS fragment libraries, one of many PFAS-specific resources available within Compound Discoverer software, to annotate fragments within acquired MS2 spectra of two unknown compounds and then identify them as PFAS that were outside the targeted quantitation method." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_3_e523e1cdc9.jpg 233w," sizes="100vw" width="1520" height="1016"></p><p>The goal of discovery is not simply to generate more data. It is to reduce complexity and identify the most relevant candidates for further investigation with a defined level of confidence.</p><h3><strong>From discovery to follow-up</strong></h3><p>One of the most important questions in non-targeted analysis is what happens after candidate identification.</p><p>Discovery workflows can identify interesting candidates, but those candidates need to move into a practical follow-up process. Scientists may want to monitor them across additional samples, estimate relative or semi-quantitative abundance, trend their presence over time, or include them in a reviewable report.</p><p>This is where the connection between Compound Discoverer software and Chromeleon CDS becomes especially valuable.</p><p>After PFAS candidates have been prioritized in Compound Discoverer software, selected components can be transferred back into Chromeleon CDS and incorporated into the Chromeleon processing methods for follow-up analysis. This allows scientists to evaluate these compounds within the same controlled environment used for targeted quantitation.</p><p>For PFAS analysis, this may support semi-quantitative follow-up for compounds where authentic standards are not yet available. While fully quantitative results require appropriate standards and validated methods, semi-quantitative workflows can provide useful estimates or relative comparisons to help prioritize further investigation.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_4_8ee8ebc2df.jpg" alt="Thermo Fisher Scientific: PFAS Analysis Beyond the Target List: Connecting Quantitation and Discovery in a Single Workflow: Figure 3. Prioritized PFAS candidates brought back into Chromeleon CDS for follow-up. Prioritized PFAS candidates from Compound Discoverer software can be brought back into Chromeleon CDS for follow-up review, semi-quantitative evaluation, and controlled reporting." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_PFAS_Analysis_Beyond_the_Target_List_Connecting_Quantitation_and_Discovery_in_a_Single_Workflow_4_8ee8ebc2df.jpg 245w," sizes="100vw" width="1512" height="751"></p><p>This is the key workflow advantage: discovery does not stop at identification. It can feed directly into a practical analytical process.</p><h3><strong>Why this matters</strong></h3><p>PFAS analysis increasingly requires laboratories to balance routine testing with exploratory investigation.</p><p>A connected workflow between Chromeleon CDS and Compound Discoverer software supports this by:</p><ul><li>Combining targeted quantitation and non-targeted discovery to characterize samples more completely</li><li>Reducing manual data transfer and workflow fragmentation</li><li>Enabling discovery results to be reviewed and reported within a controlled CDS environment</li><li>Supporting investigation of emerging PFAS investigations without immediate method redevelopment</li><li>Bridging research-driven insights with routine analytical workflows</li></ul><p>This reflects a broader shift in analytical science, where the value lies not only in generating data, but in connecting workflows and turning data into actionable information.</p><h3><strong>Moving PFAS analysis forward</strong></h3><p>PFAS analysis is evolving from a fixed target list toward a more comprehensive understanding of complex chemical mixtures.</p><p>Laboratories must quantify known compounds while remaining prepared to identify and evaluate what lies beyond them.</p><p>By connecting <strong>Compound Discoverer software </strong>with<strong> Chromeleon CDS</strong>, laboratories can move seamlessly from targeted quantitation to discovery and into structured follow-up. The result is a more complete view of PFAS contamination and a more efficient path from data to insight.</p><h3><strong>Learn more</strong></h3><p>Explore how <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1759"><strong>Compound Discoverer software</strong></a> and<a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/76"><strong> Chromeleon CDS </strong></a>can help connect targeted quantitation and non-targeted discovery workflows for <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/environmental/environmental-contaminant-analysis/emerging-contaminants/pfas-testing.html"><strong>PFAS analysis</strong></a>.</p><blockquote><p><strong>Visit us on&nbsp;</strong><a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/"><strong><u>LinkedIn:</u></strong></a><strong>&nbsp;</strong>#PFAS #Chromeleon #CompoundDiscoverer #Environmental</p></blockquote>

PFAS Analysis Beyond the Target List: Connecting Quantitation and Discovery in a Single Workflow

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Waters Sample & Collection Plate

Waters 96-well and 384-well plates are adherent to ANSI and SBS standards and are suitable for LC autosamplers and liquid handling robots.

<div><div><h4><strong>Broad selection of high-quality sample and collection plates</strong></h4></div></div><div><div><p><strong>Waters 96-well and 384-well plates are adherent to ANSI and SBS standards and are suitable for LC autosamplers and liquid handling robots. Waters plates are made from polypropylene (PP) for chemical resistance, and specialty 96-well plates with glass inserts are available for samples that are not suitable for polypropylene containers.</strong></p><h3><strong>Overview</strong></h3><div><div><div><div><div><div><ul><li>96- and 384-well collection plates and sample plates are SBS/ANSI compliant for robotic compatible systems&nbsp; &nbsp; &nbsp; &nbsp;</li><li>&nbsp;Constructed of polypropylene (PP) for chemical resistance</li><li>&nbsp;Glass inserts available for samples requiring glass surfaces</li><li>&nbsp;Heat seals and cap mats for plates and glass inserts</li></ul></div></div><div><div><h4><strong>Recommended Use</strong></h4><p>For general high-throughput LC and LC-MS work and for integration in (semi)-automated sample handling workflows.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/img_Waters_Sample_and_Collection_Plate_709693fdab.jpg" alt="imgWaters Sample &amp; Collection Plate." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_img_Waters_Sample_and_Collection_Plate_709693fdab.jpg 245w," sizes="100vw" width="1024" height="493"></p><h3><strong>Cap Mats</strong></h3><h4><strong>Silicone/PTFE &amp; Polypropylene Cap Mats</strong></h4><ul><li>Non-slit storage mats for long-term sample storage</li><li>Pre-slit silicone/PTFE and polypropylene cap mats for use in autosamplers for proper venting and sample draw accuracy with excellent resealing characteristics</li><li>Silicone/PTFE &amp; Polypropylene Cap Mats</li></ul><h3><strong>Heat Seals</strong></h3><h4><strong>Clear Polyester Heat Seals</strong></h4><ul><li>Can be used with most solvents &amp; buffers over a -80 °C to 80 °C temperature range</li><li>Requires a heat sealer to apply</li></ul><h4><strong>Aluminum Foil Heat Seals</strong></h4><ul><li>Made from a polyester/aluminum laminate</li><li>Ideal choice for long term storage to reduce evaporative loss</li><li>Aluminum layer reduces gas permeability of the seal</li><li>Can be used at temperatures from -200 °C to 90 °C</li><li>Requires a heat sealer to apply</li></ul><h4><strong>Adhesive Seals</strong></h4><ul><li>A polyolefin film with synthetic rubber adhesive</li><li>Ideal for use with protein and peptide samples and buffers</li><li>Resistant to small concentrations (0% to 30%) of polar organic solvents</li><li>Can be used at temperatures from -80 °C to 80 °C</li><li>Requires a heat sealer to apply</li></ul></div></div></div></div></div></div></div></div>

Waters CERTIFIED HPLC Vials

Reducing failed assays from unknown contaminant peaks and limiting instrument downtime from out of specification vials is a priority for your lab. Waters sample vials can help you do just that.

<div><div><h4><strong>Meet your analytical challenges with the right vial.</strong></h4></div></div><div><div><p><strong>The right vial.&nbsp;</strong><span><strong>A world of difference.</strong></span></p><p>Reducing failed assays from unknown contaminant peaks and limiting instrument downtime from out of specification vials is a priority for your lab. Waters sample vials can help you do just that.</p><p>All Waters sample vials are held to the tightest dimensional tolerances. Manufactured via a 2-, 3-, or 4-step certification process, we ensure that Waters Certified Vials meet and maintain specified vial dimensions, levels of cleanliness and adsorption properties from batch to batch.&nbsp;Find the right vial for your application and autosampler - use our <a target="_blank" rel="noopener noreferrer" href="https://find.waters.com/VialSelector/"><strong>Vial Selector</strong></a> to find your perfect match.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CERTIFIED_Vials_118649175c.jpg" alt="Waters CERTIFIED Vials: Table 1. Vials for Routine and Advanced Analyses." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CERTIFIED_Vials_118649175c.jpg 245w," sizes="100vw" width="1226" height="754"></p><h3><strong>Waters LCMS CERTIFIED</strong></h3><p><span><strong>✅ </strong></span>Dimensional Test</p><p><span><strong>✅ </strong></span>Septum GC Test</p><p><span><strong>✅ </strong></span>HPLC UV Test</p><p><span><strong>✅ </strong></span>MS Scan</p><ul><li>Low MS interferences from known residues &amp; polymers</li><li>For routine QC and LC-MS analysis with lower analyte concentrations from 10-100s ng/ml.</li></ul><h3><strong>Waters TruView<sup>TM</sup> LCMS CERTIFIED</strong></h3><p><span><strong>✅ </strong></span>Dimensional Test</p><p><span><strong>✅ </strong></span>Septum GC Test</p><p><span><strong>✅ </strong></span>HPLC UV Test</p><p><span><strong>✅ </strong></span>MS Scan</p><p><span><strong>✅&nbsp;</strong></span>Low Adsorption Test</p><div><div><ul><li>Lowest MS interferences from known residues &amp; polymers</li><li>For high-end LC-MS analysis and quantification of analytes with a concentration of below 1 ng/ml with reduced adsorption of polar analytes.</li></ul><h3><strong>Waters CERTIFIED</strong></h3><p><span><strong>✅ </strong></span>Dimensional Test</p><p><span><strong>✅ </strong></span>Septum GC Test</p><p><span><strong>✅ </strong></span>HPLC UV Test</p><div><div><ul><li>No UV interferences caused caused by manufacturing and packaging residues</li><li>For routine QC LC-UV analysis&nbsp;of compounds in µg/mL&nbsp;concentrations.&nbsp;</li></ul><h3><strong>MAXPEAK QuanRecovery</strong></h3><ul><li>For high recovery peptide and protein quantification workflows. Reduces sample losses due to non-specific binding (NSB) and ionic interactions.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CERTIFIED_Vials_2_4fba532335.jpg" alt="Waters CERTIFIED Vials: Vials Shapes." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CERTIFIED_Vials_2_4fba532335.jpg 245w," sizes="100vw" width="1261" height="617"></p><div><div><h3><strong>Control data quality risks and reduce unexpected or ghost peaks</strong></h3><p>Don’t let the presence of background noise impact your results, causing data quality risks and unexpected or ghost peaks. Background noise can come from many factors.&nbsp;One of the most important factors are vial and septa cleanliness, which introduces contamination peaks. Ghost peaks are introduced by analyte degradation that can be triggered by significant pH changes in the diluent. In this chromatogram, the top shows normal behavior, and the bottom chromatogram shows ghost peaks.</p><p>Waters Certified vials are cleanliness tested so you don’t have to worry about contaminant peaks. The TruView pH Control Vials are certified to minimize and control a pH shift away from the neutral.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CERTIFIED_Vials_4_7749301ba1.jpg" alt="Waters CERTIFIED Vials: Prevent the appearance of unexpected peaks during LC/UV analysis with Waters LC/GC Certified Vials as a control strategy." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CERTIFIED_Vials_4_7749301ba1.jpg 245w," sizes="100vw" width="1024" height="535"></p><h3><strong>More time on development. Less on troubleshooting</strong></h3><p>Stop losing valuable development time on troubleshooting due to analyte loss from non-specific adsorption. Waters TruView pH Control LCMS Certified Vials have the lowest adsorptive losses on the market, and you can rely on them to provide consistency from batch to batch and vial to vial. This chromatogram shows a comparison of adsorptive loss of chlorhexidine in a variety of vials.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CERTIFIED_Vials_5_d3e42fa7f2.jpg" alt="Waters CERTIFIED Vials: Comparison of adsorptive loss of chlorhexidine acetate in a variety of vials." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CERTIFIED_Vials_5_d3e42fa7f2.jpg 245w," sizes="100vw" width="1024" height="633"></p><div><div><h3><strong>Ideal for demanding MS work</strong></h3><p>LCMS Certified Vials are suited for analyte concentrations in the 100s ng/mL concentration. Waters LCMS Certified Vials are tested by MS scans from 250 to 1000 <i>m/z</i> with specifications set on the total ion count and presence of clusters in the high mass range. This chromatogram shows a comparison of typical MS scans of Waters LCMS Certified Vials to vials of other sources and clean solvents.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CERTIFIED_Vials_3_4869fbf57a.jpg" alt="Waters CERTIFIED Vials: Comparing typical MS scans of Waters LCMS Certified Vials, with vials from other sources." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CERTIFIED_Vials_3_4869fbf57a.jpg 245w," sizes="100vw" width="1024" height="406"></p><div><div><h3><strong>Stay in control of your samples' pH</strong></h3><p>The analysis of low abundance species, pH sensitive analytes or not yet well understood compound mixtures benefit from consistent and well controlled vial surfaces. The glass surface of TruView pH Control LCMS Certified Vials minimizes pH shifts away from the neutral and thus eliminates unwanted reactions and resulting ghost peaks.&nbsp;Ghost peaks are very difficult to troubleshoot.</p><p>Waters TruView pH Control LCMS Certified vials were used to analyze two compounds, drospirenone and lovastatin. The TruView pH Control LCMS Certified Vials show little to no degradant formed over the time tested. Standard glass vials showed considerable degradant formation over the same testing time with irreproducible formation of the degradant across different vials.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CERTIFIED_Vials_6_4dc330d849.jpg" alt="Waters CERTIFIED Vials: UV peak areas of lovastatin degradant across replicate vials and different vial types. Error bars indicate standard deviation for the replicate injection peak area." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CERTIFIED_Vials_6_4dc330d849.jpg 245w," sizes="100vw" width="1024" height="605"></p><div><div><h3><strong>Reducing adsorption starts here</strong></h3><p>Inconsistent analytical results from small molecule analyte adsorption onto the surface of glass vials can be a problem at today’s low analyte concentrations. Some scientists have even experienced total analyte loss with samples stored in vials for an extended time. The vial chemistry, specifically the&nbsp;glass surface, is the major contributor to this problem.</p><p>Polar analytes&nbsp;may adsorb to the glass vial surface by polar-polar interaction and/or ion exchange mechanisms, thereby reducing the overall amount of sample in solution. TruView Certified LCMS Vials&nbsp;reduce this problem dramatically due to a stringent manufacturing process. The process yields a glass surface that has a lower concentration of free ions on the surface.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CERTIFIED_Vials_8_064003b933.jpg" alt="Waters CERTIFIED Vials: Nortriptyline at 1 ppb concentration in several vials. The tall peak is the initial injection; the smaller peak (overlaid) is the four-hour injection. The first two chromatograms are competitor’s vials. The third chromatogram is for the Waters TruView LCMS Certified vial. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CERTIFIED_Vials_8_064003b933.jpg 245w," sizes="100vw" width="1912" height="1182"></p><div><div><h3><strong>No analyte left behind</strong></h3><p>Instrument development is yielding more sensitive mass spectrometers. With more sensitive instruments, analytes can be detected at pg/mL levels. At these concentrations, understanding adsorption issues is critical for good quantitative results. We tested adsorption loss for the major manufacturers and saw high sample loss across manufacturers and clear and amber glass types – with significantly less loss with Waters TruView Autosampler Vials.</p><h4><strong>Average Analyte Loss</strong></h4><p><strong>Manufacturer: Clear Glass / Amber Glass</strong></p><ul><li><strong>Vendor A</strong>: 55% / 69%</li><li><strong>Vendor B</strong>: 25% / 25%</li><li><strong>Vendor C</strong>: 54% / 74%</li><li><strong>Vendor D</strong>: 18% / 59%</li><li><strong>Vendor E</strong>: 75% / Not tested</li><li><strong>Waters TruView LCMS Certified Vial</strong>: 1% / 6%</li></ul></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div>

Waters QuanRecovery HPS Vials & Plates

 QuanRecovery Vials and Plates minimize sample losses from NSB and ionic interactions, ensuring greater confidence in results and improved assay repeatability.

<div><div id="title-b8cf3c562d" data-locator="title-with-text"><h3><strong>Reduce sample losses due to non-specific binding (NSB)</strong></h3></div></div><div><div id="text-c1d5077d21" data-locator="text"><p><strong>Successfully quantitating proteins and peptides with LC–MS is often more challenging than doing so for small molecules. Their large and complex structure requires a different approach to LC–MS method development and may also cause other unique problems like nonspecific adsorption (or nonspecific binding). Proteins and peptides in solution tend to stick to the surfaces they encounter.</strong></p><p>Autosampler-ready QuanRecovery Vials and Plates with MaxPeak High Performance Surfaces (HPS)&nbsp;are designed to enable scientists like you to meet recovery, sensitivity and reproducibility challenges in LC-MS peptide and protein quantification. By reducing sample losses from non-specific binding (NSB) and ionic interactions, QuanRecovery Vials and Plates provide your lab greater confidence in analytical results and fewer long-term assay repeatability problems.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Quan_Recovery_HPS_Vials_and_Plates_Quan_Recovery_Vials_and_Plates_with_Max_Peak_HPS_ed65a9e3fe.jpg" alt="Waters QuanRecovery HPS Vials &amp; Plates: QuanRecovery Vials &amp; Plates with MaxPeak HPS." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Quan_Recovery_HPS_Vials_and_Plates_Quan_Recovery_Vials_and_Plates_with_Max_Peak_HPS_ed65a9e3fe.jpg 234w," sizes="100vw" width="1024" height="682"></p><div data-locator="section-container-body"><div><div><div data-locator="layoutcontainer-"><div><div id="text-b5ef0a0cdd" data-locator="text"><div><div id="text-62116091dc" data-locator="text"><h3 data-locator="modal-title"><strong>Improved LC-MS analysis of peptides and proteins</strong></h3><p>It can be difficult to predict peptide losses due to non-specific binding and ionic interactions, as losses can be dependent on many characteristics, including pI and hydrophobicity. Demonstrated here are the recovery of four different peptides.&nbsp;The recovery of desmopressin is not affected by the sample container, while glucagon, insulin, and melittin suffer varying losses due to non-specific binding and ionic interactions in the glass and standard polypropylene containers.&nbsp;</p><p>Only QuanRecovery Vials and Plates with MaxPeak HPS protect these peptides from loss, enabling you to achieve higher recovery and improved repeatability.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Quan_Recovery_vialky_a_platicka_pro_lepsi_analyzy_proteinu_a_peptidu_caa551b7f1/Waters-QuanRecovery-vialky-a-platicka-pro-lepsi-analyzy-proteinu-a-peptidu_l.webp" alt="Waters QuanRecovery HPS Vials &amp; Plates: A comparison of the recovery of four peptides prepared in a typical LC-MS diluent and stored in various sample containers." width="1074" height="466"></p></div></div></div></div></div></div></div></div></div></div><div><div id="text-455dc5902f" data-locator="text"><h3 data-locator="modal-title"><strong>Enhanced recovery and sensitivity at high and low sample concentrations</strong></h3><p>Biomolecule sample losses are a problem at low concentrations. However, non-specific binding and ionic interactions can also cause losses at higher concentrations. Such losses not only negatively impact the assay sensitivity, they also compromise the reproducibility, precision, and accuracy of the analysis. QuanRecovery Vials and Plates with MaxPeak HPS protect your sample, ensuring improved analytical results.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Quan_Recovery_HPS_Vials_and_Plates_Improved_recovery_and_sensitivity_at_high_and_low_sample_concentrations_with_Quan_Recovery_Plates_2816689528.jpg" alt="Waters QuanRecovery HPS Vials &amp; Plates: Improved recovery and sensitivity at high and low sample concentrations with QuanRecovery Plates." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Quan_Recovery_HPS_Vials_and_Plates_Improved_recovery_and_sensitivity_at_high_and_low_sample_concentrations_with_Quan_Recovery_Plates_2816689528.jpg 221w," sizes="100vw" width="1024" height="724"></p><h3><strong>Overview</strong></h3><div data-locator="section-container-body"><div><div><div data-locator="layoutcontainer-"><div><div id="text-9f8f97fb02" data-locator="text"><ul><li>For large molecule LC-MS bioanalysts who need the ultimate sensitivity, reproducibility and sample stability over multiple analyses.</li><li>Improve detection limits and analysis reproducibility for peptide and protein quantification with these low adsorption plates and vials.</li><li>Designed for LC-MS analysis in autosampler ready formats with low residual volumes in 700 µL 96-well plate and 300 µL sample vial formats.</li><li>Increase analyte recovery, sensitivity and reproducibility by minimizing analyte/surface interactions that can lead to sample losses with MaxPeak High Performance Surfaces (HPS).</li><li>Suitable for workflows with ACQUITY Premier Systems and Columns.</li></ul></div></div><div><div id="text-b5ef0a0cdd" data-locator="text"><h3><strong>Recommended Use</strong></h3><p>To minimize sample losses due to non-specific binding (NSB) and ionic interactions.</p></div></div></div></div></div></div></div></div>

Waters Oasis SPE products

Waters Oasis SPE products come in a broad range of sorbents and an array of formats. With Oasis products, you can meet a wide variety of sample preparation needs.

<h4><strong>Faster, cleaner, and simpler SPE starts here</strong></h4><p>Analytical chemists worldwide use SPE to remove interfering matrix compounds and enrich the analyte of interest to ensure the generation of high-quality data.</p><p>Waters Oasis SPE products come in a broad range of sorbents and an array of formats. With Oasis products, you can meet a wide variety of sample preparation needs, from simple and fast sample matrix clean-up to more complex sample preparation challenges.</p><p>Oasis SPE products deliver unmatched purity, consistency, and quality for sample analysis. Along with seven different SPE chemistries, Oasis SPE products use universal to customized protocols to handle your unique sample.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Oasis_SPE_kolonky_2_967d461f2e/Waters-Oasis-SPE-kolonky-2.jpg" alt="Waters: Oasis SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Oasis_SPE_kolonky_2_967d461f2e/thumbnail_Waters-Oasis-SPE-kolonky-2.jpg 245w," sizes="100vw" width="950" height="416"></p><h4><strong>Overview</strong></h4><ul><li>Fully water-wettable sorbents increase reproducibility and enable elimination of condition and equilibration steps</li><li>Co-polymer pH stable from 0–14 with no secondary silanol activity</li><li>Optimal sensitivity for small molecule and therapeutic peptide analysis</li><li>Reduced matrix effects for LC-MS/MS analyses</li><li>Unsurpassed selectivity</li><li>Consistent retention and capacity, yielding the highest SPE recovery</li><li>Reduces variability in analytical results and increases robustness of analysis</li><li><strong>Recommended Use: </strong>For the fastest, cleanest, and simplest approach to SPE-LC-MS/MS method development.</li></ul><h4><strong>Solid-Phase Extraction Chemistries</strong></h4><h5><strong>Oasis PRiME HLB</strong></h5><ul><li>Multi-purpose hydrophilic-lipophilic balanced reversed phase sorbent for acids, bases, and neutrals</li><li>Cleaner samples in fewer steps with simple three-and two-step protocols, no SPE expertise required</li><li>Removes 95% of common matrix interferences, such as proteins, salts, phospholipids, and pigments</li><li>No sorbent conditioning or equilibration steps required</li><li>Faster, more predictable analysis time</li><li>Ideal for reversed-phase SPE clean-up of samples in routine analysis.</li></ul><h5><strong>Oasis PRiME MCX</strong></h5><ul><li>Mixed-mode Cation eXchange sorbent to selectively retain, concentrate and elute basic compounds</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Cleaner samples in fewer steps with simple and fast 3 and 4-step protocols</li><li>Removes 99% of phospholipids from complex sample matrices</li><li>No sorbent conditioning or equilibration steps required</li><li>Faster, more predictable analysis time</li><li>Highly reproducible target analyte recoveries</li><li>Ideal for basic compound specificity while removing matrix interferences in a simple clean-up.</li></ul><h5><strong>Oasis HLB</strong></h5><ul><li>Multi-purpose hydrophilic-lipophilic balanced reversed phase sorbent</li><li>Provides high capacity for acids, bases, and neutrals</li><li>Ideal for ultra-high capacity of very polar compounds.</li></ul><h5><strong>Oasis MCX</strong></h5><ul><li>Mixed-mode Cation eXchange sorbent for bases</li><li>Increased analyte specificity and sensitivity</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Ideal for selective retention of basic analytes.</li></ul><h5><strong>Oasis MAX</strong></h5><ul><li>Mixed-mode Anion eXchange for acids</li><li>Increased analyte specificity and sensitivity</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Ideal for selective retention of acidic analytes.</li></ul><h5><strong>Oasis WAX</strong></h5><ul><li>Weak Anion eXchange mixed-mode sorbent for strong acidic compounds</li><li>Increased analyte specificity and sensitivity</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Ideal for improved retention of strong acidic compounds.</li></ul><h5><strong>Oasis WCX</strong></h5><ul><li>Weak Cation eXchange mixed-mode sorbent for strong basic compounds</li><li>Increased analyte specificity and sensitivity</li><li>Reversed-phase and ion-exchange functionality for orthogonal sample preparation</li><li>Ideal for improved retention of all basic analytes, especially strong bases.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Sorbenty_Oasis_SPE_na_bazi_kopolymeru_Oasis_HLB_c8ffee39b3/Waters-Sorbenty-Oasis-SPE-na-bazi-kopolymeru-Oasis-HLB.png" alt="Waters: Oasis Sorbents" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Sorbenty_Oasis_SPE_na_bazi_kopolymeru_Oasis_HLB_c8ffee39b3/thumbnail_Waters-Sorbenty-Oasis-SPE-na-bazi-kopolymeru-Oasis-HLB.png 211w," sizes="100vw" width="1511" height="1119"></p><h4><strong>Oasis Formats</strong></h4><h5><strong>Oasis μElution Plate</strong></h5><ul><li>Patented μElution plate design</li><li>No evaporation and reconstitution necessary due to elution volumes as low as 25 µL</li><li>Ideal for SPE clean-up and analyte enrichment of small sample volumes</li><li>Compatible with most liquid-handling robotic systems for automated, reliable high-throughput SPE (HT-SPE)</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_m_Elution_Plate_aa7e36a4c1.jpg" alt="Waters: Oasis µElution Plate" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_m_Elution_Plate_aa7e36a4c1.jpg 245w," sizes="100vw" width="502" height="260"></p><h5><strong>Oasis 96-Well Sample Extraction Plates</strong></h5><ul><li>Innovative, award-winning two-stage well design</li><li>High throughput and high recovery</li><li>Compatible with most liquid-handling robotic systems for automated, reliable high-throughput SPE (HT-SPE)</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_96_Well_Sample_Extraction_Plates_9528d532b9.jpg" alt="Waters: Oasis 96-Well Sample Extraction Plates" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_96_Well_Sample_Extraction_Plates_9528d532b9.jpg 245w," sizes="100vw" width="525" height="329"></p><h5><strong>Oasis Syringe-Barrel Cartridges</strong></h5><ul><li>Ultra-clean syringe barrel and frits</li><li>Available in cartridges ranging from 1 cc to 60 cc</li><li>Flangeless syringe-barrel cartridges available in 1 cc, 3 cc, and 6 cc sizes</li><li>Plus-style cartridges with Luer inlet hub and outlet tip, compatible with hand-held syringes</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_Syringe_Barrel_Cartridges_961aa06b8a.jpg" alt="Waters Corporation: Oasis Syringe-Barrel Cartridges" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_Syringe_Barrel_Cartridges_961aa06b8a.jpg 245w," sizes="100vw" width="503" height="311"></p><h5><strong>Oasis Glass Cartridges</strong></h5><ul><li>Ultra-clean glass syringe with Teflon® frit</li><li>For trace level detection and analysis at part-per-trillion (PPT) levels</li><li>Available in 5 cc (200 mg) configuration</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_Glass_Cartridges_5e96319c7f.jpg" alt="Waters: Oasis Glass Cartridges" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_Glass_Cartridges_5e96319c7f.jpg 188w," sizes="100vw" width="409" height="339"></p><h5><strong>Oasis On-Line Columns and Cartridges</strong></h5><ul><li>For rugged, reproducible, and ultra-fast on-line analysis</li><li>Wide choice of configurations, particle sizes, and sorbent chemistries</li><li>Available with all six patented Oasis sorbents—PRiME HLB, HLB, MCX, MAX, WCX, and WAX</li><li>High recovery and reproducible results for a wide range of compounds</li><li>Cartridge format for use with Spark Holland Prospekt-2™/Symbiosis™ systems also available</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_On_Line_Columns_and_Cartridges_ba02114c92.jpg" alt="Waters: Oasis On-Line Columns and Cartridges" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_On_Line_Columns_and_Cartridges_ba02114c92.jpg 168w," sizes="100vw" width="415" height="386"></p><h4><strong>Sample preparation, improved</strong></h4><p>Improving various aspects of chromatography and mass spectrometry analyses often starts with sample preparation. &nbsp;Solid-phase extraction (SPE) or "liquid-solid phase extraction" is a sample preparation technology that uses particles of chromatographic packing material, usually in a cartridge or 96-well plate format, to chemically separate different analytes and aid in removing matrix components in a sample. &nbsp;</p><p>SPE is a proven sample preparation tool for simple sample matrix removal, including common interferences like proteins, salts, fats and phospholipids. This matrix removal provides many benefits, including matrix effect reduction and an increase in sample concentration and analyte specificity. Matrix removal also significantly simplifies chromatographic complexity by reducing the number of interfering peaks in the LC and reducing signal suppression by providing clean samples for the MS.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_SPE_LC_MS_MS_chromatogram_of_1_pgm_L_of_alprazolam_from_rat_plasma_8b7275b25b.jpg" alt="Waters: Oasis SPE - LC-MS-MS chromatogram of 1 pgmL of alprazolam from rat plasma" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_SPE_LC_MS_MS_chromatogram_of_1_pgm_L_of_alprazolam_from_rat_plasma_8b7275b25b.jpg 169w," sizes="100vw" width="1024" height="946"></p><h4><strong>Get the cleanest extracts with the Oasis 2x4 method</strong></h4><p>Having a variety of tools for method development allows separation scientists to optimize protocols for maximum sensitivity and selectivity. The Oasis 2x4 method for SPE sorbent and protocol selection for acids, bases, and neutrals produces the cleanest extracts – all by eliminating matrix interferences and sample variability. Get the flexibility to extract acids, bases, and neutrals with high SPE recoveries while removing matrix components that may interfere with analysis with our two protocols and four sorbents. By following the simple steps outlined in the Oasis 2x4 method, you can achieve high recoveries and the cleanest extracts.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Oasis_2x4_protokol_pro_vyvoj_metody_c417be9006/Waters-Oasis-2x4-protokol-pro-vyvoj-metody.jpg" alt="Waters: Oasis SPE - Oasis 2 x 4 method development protocol." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Oasis_2x4_protokol_pro_vyvoj_metody_c417be9006/thumbnail_Waters-Oasis-2x4-protokol-pro-vyvoj-metody.jpg 242w," sizes="100vw" width="1519" height="979"></p><h4><strong>Isolate your compounds with the Oasis HLB method</strong></h4><p>Solid-phase extraction (SPE) method development starts with knowledge of the sample matrix—the nature and relative concentration of the analytes of interest and potentially interfering endogenous compounds. The right sorbent and solvents are selected for load, wash, and elute steps, resulting in the creation of rugged, reliable, and selective protocols. The Oasis HLB method is a generic protocol used to isolate a broad range of acidic, basic, and neutral compounds from a wide spectrum of matrices.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Oasis_SPE_Generic_Method_Protocol_with_Oasis_HLB_SPE_83d684704d.jpg" alt="Waters: Oasis SPE - Generic Method Protocol with Oasis HLB SPE" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Oasis_SPE_Generic_Method_Protocol_with_Oasis_HLB_SPE_83d684704d.jpg 139w," sizes="100vw" width="1024" height="1153"></p><h4><strong>Multiple contaminant analysis with the 2-step pass-through protocol</strong></h4><p>If you are doing multiple residue screening of veterinary drugs, pesticides and mycotoxins in various food matrices, you may be facing a high concentration of organic solvents. With our simple 2-step protocol (pass-through SPE), you can remove matrix interferences quickly if your samples contain a high concentration of organic solvent(s) and concentration and/or salt removal is not required. It can be used as the clean-up method following QuEChERS extraction, saving you significant time compared to the traditional dispersive SPE method - all while achieving superior clean-up.</p><p><strong>To do pass-through SPE, Waters offers 2 convenient and flexible formats:</strong></p><ul><li><strong>Traditional-style cartridges</strong> compatible with vacuum and positive pressure manifolds&nbsp;</li><li><strong>"Plus" style cartridges</strong> that can be used without manifolds when connected to a displacement syringe</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Oasis_SPE_kolonky_3_b4da6242de/Waters-Oasis-SPE-kolonky-3.jpg" alt="Waters: Oasis Plus Light and Plus Short Cartridges for 2-step pass through protocol." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Oasis_SPE_kolonky_3_b4da6242de/thumbnail_Waters-Oasis-SPE-kolonky-3.jpg 152w," sizes="100vw" width="1308" height="1342"></p>

Products from category Consumables distributed by Waters Corporation (Europe) - page 1

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