The KNAUER RF-20A Fluorescence Detector is a highly sensitive fluorescence detector designed for demanding HPLC applications requiring exceptional selectivity and trace-level detection. Covering a wavelength range from 200 to 650 nm, the RF-20A delivers outstanding sensitivity, excellent reproducibility, and reliable operation for both routine and advanced chromatographic analyses.
With a signal-to-noise ratio of at least 1200 for the water Raman band, the detector is ideally suited for trace-level determinations in pharmaceutical, environmental, food, and biochemical applications. A fast 10 ms response time supports high-speed chromatography without compromising chromatographic resolution. Time-programmed wavelength switching allows optimal excitation and emission settings throughout the run, enabling highly sensitive multi-component analysis.
Key Features
- High-sensitivity fluorescence detector for HPLC applications
- Wavelength range from 200 to 650 nm
- Signal-to-noise ratio ≥1200 (water Raman band)
- Excellent performance for trace-level analysis
- Fast 10 ms response time
- Time-programmable wavelength switching
- Multi-component analysis at optimal wavelengths
- Long-life xenon lamp (approx. 2,000 hours)
- Front-accessible lamp and flow cell
- No optical adjustment required after lamp replacement
- Minimal maintenance requirements
- Stand-alone operation via keypad and display
- Compatible with ClarityChrom®, OpenLAB®, and PurityChrom® software
- Easy integration into existing LC systems
Technical Specifications
Detection System
- Detection channels: 1
- Number of signals: 1
- Light source: Xenon lamp
- Wavelength range: 200–650 nm
- Spectral bandwidth: 20 nm
- Wavelength accuracy: ±2 nm
- Wavelength precision: ±0.2 nm
Sensitivity
- Signal-to-noise ratio: ≥1200 (water Raman band)
- Sensitivity settings:
- HIGH (×1)
- MED (×32)
- LOW (×1024)
Flow Cell
- Standard flow cell included
- Flow cell volume: 12 µL
- Wetted materials:
- SUS316L stainless steel
- PTFE
- Quartz
Signal Processing
- Response time: 10 ms
- Gain settings: ×1, ×4, ×16
Functions
- Autozero function
- Baseline shift function
- Time-programmed wavelength switching
Communication
- RS-232 interface
- Analog output
- Software control
- Stand-alone operation via keypad and display
Environmental Conditions
- Operating temperature: 4–35 °C
- Relative humidity: 20–85% (non-condensing)
Power and Dimensions
- Power supply: AC 220–240 V
- Frequency: 50/60 Hz
- Power consumption: 400 VA
- Dimensions (W × H × D): 260 × 210 × 420 mm
- Weight: 16 kg
Applications
- Pharmaceutical analysis
- Impurity profiling
- Environmental monitoring
- Food and beverage testing
- Amino acid analysis
- Vitamin analysis
- PAH analysis
- Biomolecule detection
- Trace contaminant determination
- Research and quality control laboratories
