Polar Analytes: C18 Didn’t Work, Now What?

Thinking Outside the C18 Box 1 Modes of HPLC • Reversed-phase • C18, C8, other non-polar phases • Phenyl, PFP, other more polar phases • Ion Pairing • HILIC • Silica • Amide • Zwitterionic • Hydroxyl-based • Chiral •…

Too Polar for Reversed Phase – What Do You Do? June 20, 2013 Mark Powell Columns and Consumables Technical Support Page 1 C8 or C18 Doesn’t Always Do the Job • Typical reversed phase conditions involve water/buffer with 5 to…

HILIC Chromatography: When and How? Golnar Javadi Applications Engineer LC Columns and Consumables Technical Support January 25, 2022 Agenda 1 What is HILIC and when should you consider it? 2 HILIC method development • • 3 Tips and tricks for…

Application Note Small Molecule Pharma Retaining and Separating Polar Molecules—A Detailed Investigation of When to Use HILIC Versus a Reversed-Phase LC Column Author Anne Mack Agilent Technologies, Inc. Abstract This work examines when to use hydrophilic interaction LC (HILIC) and…