Retaining and Separating Polar Molecules—A Detailed Investigation of When to Use HILIC Versus a Reversed-Phase LC Column

HILIC Chromatography: When and How? Golnar Javadi Applications Engineer LC Columns and Consumables Technical Support January 25, 2022 Agenda 1 What is HILIC and when should you consider it? 2 HILIC method development • • 3 Tips and tricks for…

Why Use HILIC and HIC Chromatography? Theory and method development strategies Mohit Patel, PhD LC Applications Engineer October 8, 2024 1 10/08/2024 DE-001530 AGILENT CONFIDENTIAL Agenda 1 What is HILIC and when should you consider it? 2 How to approach…

How Do I Choose? A guide to HPLC column selection Mark Powell Columns and Supplies Technical Support 16 December 2021 Overview How do I choose? • Efficiency • Particle size • Column length • Selectivity • Bonded phase • Mobile…

Thinking Outside the C18 Box 1 Modes of HPLC • Reversed-phase • C18, C8, other non-polar phases • Phenyl, PFP, other more polar phases • Ion Pairing • HILIC • Silica • Amide • Zwitterionic • Hydroxyl-based • Chiral •…