Too Polar for Reversed Phase – What Do You Do?

Polar Analytes: C18 Didn’t Work, Now What? Mark Powell Agilent Technologies Technical Support Page 1 C18 Doesn’t Always Work… Page 2 Pore Dewetting or Phase Collapse •Alkyl phases such as C8 or C18 can exhibit poor retention or reproducibility of…

Thinking Outside the C18 Box 1 Modes of HPLC • Reversed-phase • C18, C8, other non-polar phases • Phenyl, PFP, other more polar phases • Ion Pairing • HILIC • Silica • Amide • Zwitterionic • Hydroxyl-based • Chiral •…

LC_CSG_00_Cover_LC Column Selection Guide 2012 5/8/12 5:43 PM Page 1 Agilent HPlC Column Selection guide SOLUTIONS FOR SMALL MOLECULE SEPARATIONS For more information Buy online: www.agilent.com/chem/store u.S. and Canada 1-800-227-9770 [email protected] europe [email protected] Asia Pacific [email protected] this information is subject…

LC_CSG_00_Cover_LC Column Selection Guide 2012 5/8/12 5:43 PM Page 1 Agilent HPlC Column Selection guide SOLUTIONS FOR SMALL MOLECULE SEPARATIONS For more information Buy online: www.agilent.com/chem/store u.S. and Canada 1-800-227-9770 [email protected] europe [email protected] Asia Pacific [email protected] this information is subject…