A Single Injection LC-MS Analysis Scheme for Simultaneous Analysis of Biotherapeutics and Host-Cell Impurities via Online Digestion LC-MS/MS

Increasing sensitivity of high throughput host cell protein analysis on a novel highresolution accurate mass platform Eugen Damoc1; Tabiwang N. Arrey1; Anna Pashkova1; Eduard Denisov1; Kai Scheffler2; Kristina Srzentić3 1Thermo Fisher Scientific (Bremen) GmbH, Bremen, Germany; 2Thermo Fisher Scientific, Germering,…

DATA INDEPENDENT LC-MS ASSAYS FOR IDENTIFICATION, QUANTIFICATION AND MONITORING OF HOST CELL PROTEINS IN MONOCLONAL ANTIBODIES Catalin Doneanu, Michael Daly, Sean Wu, Scott Berger and Ying Qing Yu Waters Corporation, Milford, MA, USA OVERVIEW HCP Discovery Assay (3B) HCP Monitoring…

[ APPLICATION NOTE ] A Comprehensive Approach for HCP Identification, Quantification, and Monitoring Based on a Single Dimension (1D) LC Separation Catalin Doneanu, 1 Sarah Lennon, 2 Malcolm Anderson, 2 Ian Reah, 3 Mal Ross, 3 Steven Anderson, 3 Ian…

PO-CON1486E Online affinity and digestion: A flexible and robust tool for the characterization and quantification of proteins ASMS 2014 WP161 David Colquhoun1; Mohamed Nazim Boutaghou1; Rachel Lieberman1; Brian Feild1; Kevin W. Meyer2; Scott Kuzdzal1 1 Shimadzu Scientific Instruments, Columbia, MD;…