Expanding the Antibody-Oligo Conjugate (AOC) Characterization Toolbox: Part 3- Partial and Complete AOC Conjugation Site Determination

Comprehensive LC-MS Analytical Assays for Antibody-Oligonucleotide Conjugate (AOC) and siRNA Linker-Payload Characterization Samantha Ippoliti1; Brad Williams1; Ying Qing Yu1; Connor Brandenburg; Tara MacCulloch; Michelle Chen2; Sophia Kenrick2 1 Waters Corporation, Milford, MA; 2Waters Corporation, Goleta, California INTRODUCTION METHODS Antibody-oligo conjugates…

Application Note Expanding the Antibody-Oligo Conjugate (AOC) Characterization Toolbox: Part 1- OAR Analysis and Conjugation Process Monitoring Samantha Ippoliti, Ying Qing Yu, Connor Brandenburg, Tara MacCulloch, Michelle Chen Waters Corporation, United States Note: This is the first application note in…

Application Note Expanding the Antibody-Oligo Conjugate (AOC) Characterization Toolbox: Part 2Denaturing and Non-denaturing Analysis of siRNA Payload Samantha Ippoliti, Ying Qing Yu, Connor Brandenburg, Tara MacCulloch Waters Corporation, United States Note: This is the second application note in a three-part…

Agilent BioHPLC columns Characterization of Antibody-Drug Conjugate Critical Quality Attributes Application compendium Contents Introduction3 Featured application notes 2 PLRP-S Polymeric Reversed-Phase Column for LC/MS Separation of mAbs and ADC (5991-7163EN) 5 Intact- and Peptide-Level Characterization of Trastuzumab Emtansine In Vitro…