Increasing the Productivity of Oligonucleotide Purification through Column Scaling and Method Optimization

[ APPLICATION NOTEBOOK ] APPLICATION SOLUTIONS FOR OLIGONUCLEOTIDES Table of Contents Introduction – UPLC Analysis of Synthetic Oligonucleotides......................................................................................................................... 4 [ HPLC & UPLC Separations ] Real-Time Analysis of RNAi Duplexes ....................................................................................................................................................................... 8 Semi-Preparative Scale Single-Stranded RNA Purification .......................................................................................................................... 11 Oligonucleotide…

An Introduction to Ion-Paired Reverse-Phase Oligonucleotide Separations: From Analysis to Purification Melissa Goodlad, PhD Columns and Supplies Applications Engineer April 15, 2025 1 April 15, 2025 An Introduction to Ion-Paired Reverse-Phase Oligonucleotide Separations DE-006001 Introduction to Ion-Paired Reverse-Phase (IP-RP) Separations…

An Introduction to Ion-Paired Reverse-Phase Oligonucleotide Separations: From Analysis to Purification Melissa Goodlad, PhD Columns and Supplies Applications Engineer April 15, 2025 1 April 15, 2025 An Introduction to Ion-Paired Reverse-Phase Oligonucleotide Separations DE-006001 Introduction to Ion-Paired Reverse-Phase (IP-RP) Separations…

White Paper Biopharma Maximize Your Resources and Simplify Scale-up with AdvanceBio Oligonucleotide Columns Competitive comparison from analytical to semipreparative purification Authors Introduction Andrea Angelo P. Tripodi, Andrew Coffey, and Connor Flannery Agilent Technologies, Inc. The Agilent AdvanceBio Oligonucleotide product family…