One Pot, Mildly Acidic Digestion Protocols for Peptide Mapping Using Lys-C and RapiZyme™ Trypsin

Peptide Mapping for Biotherapeutics Strategies for Simplifying Protein Digestion A sponsored publication from Peptide Mapping Doesn’t Need to Be Complex Introducing PeptideWorks™ Tryptic Protein Digestion Kits PeptideWorks Tryptic Protein Digestion Kits uniquely deliver automatable, high efficiency, reproducible peptide maps in…

Application Note Quick and Robust Sample Preparation for Tryptic Peptide Mapping With the PeptideWorks™ Kit Using Simple, Automatable Workflows Caitlin M. Hanna, Jonathan P. Danaceau, Stephan M. Koza, Steve Shiner, Mary Trudeau Waters Corporation Abstract Here, we present an automatable…

Application Note Versatile and Rapid Digestion Protocols for Biopharmaceutical Characterization Using RapiZyme™ Trypsin Samantha Ippoliti, Nick Zampa, Ying Qing Yu, Matthew A. Lauber Waters Corporation Abstract Complete and clean trypsin digestion is achieved with an enzyme that provides a balance…

IMPROVED BIOPHARMACEUTICAL PEPTIDE MAPPING WORKFLOWS USING A NOVEL AUTOLYSIS-RESISTANT TRYPSIN ENZYME Samantha Ippoliti1, Nick Zampa1, Ying Qing Yu1, Matthew A Lauber1, Leslie Napoletano1, Scott Berger1 Waters Corporation, Milford, MA METHODS Peptide mapping provides a wealth of information about a protein…