Improved Recovery of a Lipid Conjugated Antisense Oligonucleotide from Human Plasma using the OligoWorks™ SPE Microplate Kit and an Optimized Protocol

Application Note Improved Bioanalytical Extraction of Therapeutic Antisense Oligonucleotides, Including a Lipid Conjugated ASO From Liver and Brain Tissue Using the OligoWorks™ SPE Microplate Kit Makda Araya, Mary Trudeau, Balasubrahmanyam Addepalli, Matthew A. Lauber Waters Corporation Abstract To develop and…

Application Note Quantification of a Lipid Conjugated Antisense Oligonucleotide (ASO) Extracted From Rat Plasma Using the OligoWorks™ SPE Microplate Kit on a HRMS System Nikunj Tanna, Mary Trudeau Waters Corporation Abstract Discovery bioanalysis laboratories play a critical role in the…

IMPROVED TISSUE HOMOGENIZATION AND SPE-BASED SAMPLE PREPARATIONS FOR THE QUANTITATIVE LC-MS ANALYSIS OF OLIGONUCLEOTIDE THERAPEUTICS Makda Araya; Kim Tran; Mary Trudeau; Joe Fredette; Balasubrahmanyam Addepalli; Matthew Lauber Waters Corporation, Milford, MA RESULTS INTRODUCTION Efficient extraction of drug analytes is a…

[ CONSUMABLES GUIDE BOOK ] Quantifying Oligonucleotides Sample Extraction and LC Tools for Reliable LC-MS Quantitation of Oligonucleotides A Changing Industry There has been a resurgence in synthetic oligonucleotide therapeutics in the past decade, as seen with burgeoning pipelines across…