Quantification of a Lipid Conjugated Antisense Oligonucleotide (ASO) Extracted From Rat Plasma Using the OligoWorks™ SPE Microplate Kit on a HRMS System

BIOANALYTICAL QUANTIFICATION OF A LIPID CONJUGATED ANTI-SENSE OLIGONUCLEOTIDE ON A HRMS SYSTEM Authors: Nikunj Tanna, Joe Fredette and Mary Trudeau Affiliations INTRODUCTION METHODS SPE METHOD OPTIMIZATION Oligonucleotide therapeutics (ONTs) are a key focus area for many drug developers today given…

Application Note Improved Recovery of a Lipid Conjugated Antisense Oligonucleotide from Human Plasma using the OligoWorks™ SPE Microplate Kit and an Optimized Protocol Mary Trudeau, Matthew A. Lauber Waters Corporation This is an Application Brief and does not contain a…

IMPROVED TISSUE HOMOGENIZATION AND SPE-BASED SAMPLE PREPARATIONS FOR THE QUANTITATIVE LC-MS ANALYSIS OF OLIGONUCLEOTIDE THERAPEUTICS Makda Araya; Kim Tran; Mary Trudeau; Joe Fredette; Balasubrahmanyam Addepalli; Matthew Lauber Waters Corporation, Milford, MA RESULTS INTRODUCTION Efficient extraction of drug analytes is a…

M0930-06-36 ELEMENTS OF ROBUST MODIFIED OLIGONUCLEOTIDE EXTRACTION THROUGH SPE Makda Araya, Abraham Finny, Balasubrahmanyam Addepalli and Matthew Lauber Waters Corporation CONTACT INFORMATION: [email protected] PURPOSE RESULTS Extraction and Quantification of Therapeutic Oligonucleotides (OTx) during Bioanalysis Robust extraction of modified and unmodified…