Extended Analysis of mAbs Using Ion- Exchange Chromatography on the Alliance™ iS Bio HPLC System

[ TECHNOLOGY BRIEF ] Charge Variant Analysis of Infliximab on the Alliance, ACQUITY Arc Bio, and ACQUITY UPLC H-Class Bio Liquid Chromatography Systems Using a Quaternary pH Gradient Corey E. Reed, Stephan M. Koza, and Weibin Chen, Waters Corporation, Milford,…

ADVANCING ANALYTICAL APPROACHES FOR ROBUST EVALUATION OF LARGE MOLECULE BIOLOGICS USING LIQUID CHROMATOGRAPHY Corey E. Reed, Andrew Steere, Paula Hong Waters Corporation, Milford, MA 01757 (USA) INTRODUCTION • RESULTS & DISCUSSION Size-Exclusion Chromatography (SEC) and Ion-Exchange Chromatography (IEX) are prominent…

Application Note Advancing Charge Variant Analysis with pHGradient Ion-Exchange Chromatography: Optimizing Monoclonal Antibody Characterization Using the High-pH Kit for the Alliance™ iS Bio HPLC System Corey E. Reed, Jennifer Simeone, Paula Hong Waters Corporation, United States Published on August 04,…

Characterization and Monitoring of mAb Charge Variants via Online IEX-MS Samantha Ippoliti Waters MS User Meeting ASMS 2019 Atlanta, GA June 1, 2019 ©2019 Waters Corporation 1 Agenda 1. mAb CQA Characterization 2. Reverse Phase vs Ion Exchange Chromatography 3.…