Increased sensitivity and throughput for native intact mass analysis of mAb and ADCs using an online buffer exchange column

Application note | 004331 Biopharma Automated workflow for intact antibody drug conjugates (ADCs) and DAR analysis in Chromeleon CDS Authors Application benefits Ulrik H. Mistarz1, Marcus Hoffmann2, • Automated, high-throughput capable analysis of intact antibody-drug conjugates (ADCs) and drug-to-antibody ratio…

Application note | 004167 Biopharma Charge variant analysis of cysteine-linked antibody-drug conjugates using an online multiple heart-cut 2DSCX-SEC-LC HRAM MS approach Authors Application benefits Xuepu Li¹, Xiaoxi Zhang¹, Maria Grübner², • The online multiple heart-cut two-dimensional liquid chromatography (2D-LC) system…

Imaged capillary isoelectric focusing – Mass Spectrometry (iCIEF- MS) online coupling for polatuzumab vedotin charge heterogeneity analysis using native MS Xiaoxi Zhang1, Tony Chen2, Tao Bo2, Tiemin Huang2, Min Du3. 1 Thermo Fisher Scientific, Shanghai, China, 2 Advanced Electrophoresis Solutions…

Biopharma High-sensitive multi-attribute analysis of ADCs under native conditions by using an online multiple heart-cut 2D-LC-HRAM mass spectrometry system Frank Steiner1, Maria Gruebner1, Xuepu Li2, Xiaoxi Zhang2, Min Du3 1Thermo Fisher Scientific, Germering, Germany, 2Thermo Fisher Scientific, Shanghai, China, 3Thermo…