Development of a systematic approach for improving tryptic digestion, recovery and chromatographic performance for mAb analysis

CUSTOMER APPLICATION NOTE 73826 Peptide mapping of challenging monoclonal antibodies Authors: Dan Bach Kristensen1, Trine Meiborg Sloth1, Martin Ørgaard1, Krisztina Radi2 Symphogen, Ballerup, Denmark Thermo Fisher Scientific, Hemel Hempstead, UK 1 2 Keywords: Peptide mapping, monoclonal antibody, mAb, post-translational modifications,…

APPLICATION NOTE 21834 Automated chymotrypsin peptide mapping of proteins by LC-MS Authors Amy Farrell1, Jonathan Bones1, Ken Cook 2 1 Characterisation and Comparability Laboratory, NIBRT – The National Institute for Bioprocessing Research and Training, Dublin, Ireland 2 Thermo Fisher Scientific,…

APPLICATION NOTE 21682 High-precision, automated peptide mapping of proteins Authors Application benefits Amy Farrell1, Jonathan Bones1, Ken Cook 2, Suraj Patel2, Alexander Schwahn2, Jon Bardsley2 1 Characterisation and Comparability Laboratory, NIBRT – The National Institute for Bioprocessing Research and Training,…

APPLICATION NOTE 21782 Comparison of alternative approaches to trypsin protein digestion for reproducible and efficient peptide mapping analysis of monoclonal antibodies Authors Silvia Millán-Martín, Craig Jakes, Noemi Dorival-García, Nicola McGillicuddy, Sara Carillo, Amy Farrell, Jonathan Bones National Institute for Bioprocessing…