Automated chymotrypsin peptide mapping of proteins by LC-MS

APPLICATION NOTE 21682 High-precision, automated peptide mapping of proteins Authors Application benefits Amy Farrell1, Jonathan Bones1, Ken Cook 2, Suraj Patel2, Alexander Schwahn2, Jon Bardsley2 1 Characterisation and Comparability Laboratory, NIBRT – The National Institute for Bioprocessing Research and Training,…

Table of Contents Introduction Faster and More Sensitive Protein Characterization and Quantitation Easier Digestion Faster Digestion Highly Reproducible Digestion Automation of Digestion Improving Sensitivity and Speed SMART Digest Peptide Mapping and Quantitation Compendium Peptide Mapping Peptide Quantitation Product and Method…

CUSTOMER APPLICATION NOTE 73826 Peptide mapping of challenging monoclonal antibodies Authors: Dan Bach Kristensen1, Trine Meiborg Sloth1, Martin Ørgaard1, Krisztina Radi2 Symphogen, Ballerup, Denmark Thermo Fisher Scientific, Hemel Hempstead, UK 1 2 Keywords: Peptide mapping, monoclonal antibody, mAb, post-translational modifications,…

Protein digestion for peptide mapping and quantitation Overview of SMART Digest and SMART Digest ImmunoAffinity Kits SMART Digest and SMART Digest ImmunoAffinity kits Thermo Scientific™ SMART Digest™ and the SMART Digest™ ImmunoAffinity (IA) kits are designed for biomarker and bio-therapeutic…