High-Sensitivity Oligonucleotide Analysis Using Ion-Pairing-Free HILIC and TOF LC/MS

Application Note BioPharma MS1 Oligonucleotide Characterization Using LC/Q-TOF with HILIC Chromatography Authors Introduction Peter Rye and Cody Schwarzer Agilent Technologies, Inc. The synthesis of RNA and DNA oligonucleotides (oligos) is an iterative process that, despite highly optimized chemistry, results in…

Application Note Biopharmaceuticals Analysis of Oligonucleotides Using an Ion-Pairing-Free Reversed-Phase Method with TOF LC/MS Authors Lee Bertram and Jordy Hsiao Agilent Technologies, Inc. Abstract This application note details a reversed-phase separation methodology for oligonucleotides without the use ion pairing reagents.…

Application Note BioPharma MS/MS Oligonucleotide Sequencing Using LC/Q-TOF with HILIC Chromatography Authors Guannan Li and Peter Rye Agilent Technologies, Inc. Introduction Oligonucleotides are commonly analyzed by liquid chromatography/mass spectrometry (LC/MS) in negative ion polarity mode using ion-pairing reversed‑phase (IP‑RP) methods.…

Poster Reprint ASMS 2026 MP 528 Improved Oligonucleotide LC–MS Performance through Inert Column Hardware and Alternative Separation Strategies Andrew Coffey1; Daniel Meston1; Chae-Young Ryu2; Anne Blackwell2; Jordy Hsiao4 1Agilent Technlogies, Church Stretton, United Kingdom; 2 Agilent Technologies, Seoul, South Korea;…