Analysis of Oligonucleotides Using an Ion-Pairing-Free Reversed-Phase Method with TOF LC/MS

Application Note Biopharma Analysis of Oligonucleotides Using Ion-Pairing Alternatives on the Agilent Pro iQ Plus Authors Lee Bertram and Brian Rivera Agilent Technologies, Inc. Abstract This application note presents a practical approach for medium- to high-throughput molecular confirmation of synthetic…

Application Note BioPharma MS/MS Oligonucleotide Sequencing Using LC/Q-TOF with HILIC Chromatography Authors Guannan Li and Peter Rye Agilent Technologies, Inc. Introduction Oligonucleotides are commonly analyzed by liquid chromatography/mass spectrometry (LC/MS) in negative ion polarity mode using ion-pairing reversed‑phase (IP‑RP) methods.…

Application Note Pharma & Biopharma Oligonucleotides Oligonucleotide Characterization by Agilent 1290 Infinity II Bio LC and 6545XT AdvanceBio LC/Q-TOF Oligonucleotide identity, impurities analysis and sequence determination using BioConfirm 12.0 target plus impurities (TPI) and sequence confirmation workflows Authors Introduction Bian…

Application Note High-throughput, Ion-Pairing-Free, HILIC Analysis of Oligonucleotides Using Agilent RapidFire Coupled to Quadrupole Time-of-Flight Mass Spectrometry Author Abstract Peter Rye, PhD Agilent Technologies, Inc. This application note describes a high-throughput, ion-pairing-free method for oligonucleotide characterization using the Agilent RapidFire…