Determination of Galactosamine Containing Organic Impurities in Heparin by HPAE-PAD Using the CarboPac PA20 Column

Deanna Hurum and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Key Words AminoTrap, Chondroitin Sulfates, Electrochemical Detection , Eluent Generation, RFIC-EG, USP Introduction In early 2008, unusual reactions to heparin, including hypotension, swelling of the larynx, and in some…

TECHNICAL NOTE 72225 Glycoprotein monosaccharide analysis using HPAE-PAD with manually prepared eluent Authors Sachin Patil and Jeffrey Rohrer, Thermo Fisher Scientific, Sunnyvale, CA, USA Goal To present an accurate method of determining monosaccharides in glycoproteins using high-performance anion-exchange chromatography with…

APPLICATION NOTE 72580 An improved HPAE-PAD method for glycoprotein monosaccharide determination Authors Sachin Patil and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Goal To demonstrate an improved HPAE-PAD assay for determination of the monosaccharide composition of a glycoprotein Introduction…

Lipika Basumallick and Jeffrey Rohrer, Thermo Fisher Scientific, Sunnyvale, CA, USA Key Words Carbohydrate Analysis, High Throughput, Bovine Fetuin, Human Immunoglobulin (IgG), Gold on PTFE Disposable Electrode Goal This study is intended as a guide for investigators interested in validating…