Glycoprotein monosaccharide analysis using HPAE-PAD with manually prepared eluent

APPLICATION NOTE 72580 An improved HPAE-PAD method for glycoprotein monosaccharide determination Authors Sachin Patil and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Goal To demonstrate an improved HPAE-PAD assay for determination of the monosaccharide composition of a glycoprotein Introduction…

Technical Note 53 Determination of Glycoprotein Monosaccharide Composition by HPAE-PAD Using On-Line Electrolytically Generated Eluents INTRODUCTION EQUIPMENT High-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) at a gold electrode is a convenient and sensitive technique for carbohydrate analysis. HPAE-PAD provides…

Lipika Basumallick and Jeffrey Rohrer, Thermo Fisher Scientific, Sunnyvale, CA, USA Key Words Carbohydrate Analysis, High Throughput, Bovine Fetuin, Human Immunoglobulin (IgG), Gold on PTFE Disposable Electrode Goal This study is intended as a guide for investigators interested in validating…

Lipika Basumallick, Udayanath Aich, and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Key Words Glycoprotein Monosaccharide Analysis, Gold on PTFE Disposable Electrode, Peak Fronting, HPAE-PAD Introduction The recommended technique to determine the monosaccharide content of a glycoprotein is to…