Glycoprotein Monosaccharide Analysis Using HPAE-PAD with Eluent Generation

TECHNICAL NOTE 72225 Glycoprotein monosaccharide analysis using HPAE-PAD with manually prepared eluent Authors Sachin Patil and Jeffrey Rohrer, Thermo Fisher Scientific, Sunnyvale, CA, USA Goal To present an accurate method of determining monosaccharides in glycoproteins using high-performance anion-exchange chromatography with…

APPLICATION NOTE 72580 An improved HPAE-PAD method for glycoprotein monosaccharide determination Authors Sachin Patil and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Goal To demonstrate an improved HPAE-PAD assay for determination of the monosaccharide composition of a glycoprotein Introduction…

Technical Note 53 Determination of Glycoprotein Monosaccharide Composition by HPAE-PAD Using On-Line Electrolytically Generated Eluents INTRODUCTION EQUIPMENT High-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) at a gold electrode is a convenient and sensitive technique for carbohydrate analysis. HPAE-PAD provides…

Lipika Basumallick, Udayanath Aich, and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA, USA Key Words Glycoprotein Monosaccharide Analysis, Gold on PTFE Disposable Electrode, Peak Fronting, HPAE-PAD Introduction The recommended technique to determine the monosaccharide content of a glycoprotein is to…