Sampling the human population – ultra-high- throughput plasma protein profiling (uHTPPP) sample preparation for translational proteomics

An Ultra High-throughput Plasma Protein Profiling (uHTPPP) Workflow Using a Modified Quadrupole-Orbitrap Mass Spectrometer Michelle L. Dubuke1, Sarah Trusiak1, Sitara Chauhan1, Ryan D. Bomgarden2, Sergei Snovida2, Bhavin Patel2, Emily I. Chen1. 1Thermo Fisher Scientific, Precision Medicine Science Center, Cambridge, MA.…

APPLICATION NOTE No. 73194 High-throughput plasma proteomics: A standardized and scalable workflow for quantitative protein profiling in large sample cohorts Keywords Q Exactive, Orbitrap, protein biomarker, plasma workflow, serum workflow, Evosep, high throughput proteomics workflow Authors Jing Wang1; Sarah Trusiak1;…

CASE STUDY 66002 Orbitrap Exploris 240 mass spectrometer delivers significantly increased lab productivity and reproducibility of human plasma proteome profiling from large human cohort studies “For large clinical cohort studies where hundreds of plasma samples are to be analyzed, a…

Proof of performance Orbitrap Exploris 240 mass spectrometer Plasma profiling–reliable large cohort analysis with a fully automated workflow Summary Here we present an Ultra-High Throughout Plasma Protein Profiling (uHTPPP) workflow (Figure 1), from automated sample preparation to high-resolution, accurate-mass (HRAM)…