Optimization of Crosslinked Peptide Analysis on an Orbitrap Fusion Lumos Mass Spectrometer

Thermo Scientific™ Proteome Discoverer™ using a XlinkX software node. Methods: Different amine-reactive, homobifuctional crosslinkers including disuccinimidyl Results: For both DSSO and BuUrBu, we identified over 40 BSA inter-crosslinked peptides 2 CID for DSSO. We also to less than(DSS), 20 using…

Evaluation of FAIMS Technology for Mass Spec Analysis of Chemical Cross-linked Peptides Rosa Viner1; Leigh A Foster2; Ryan D. Bomgarden2; Michael W. Belford1; Satendra Prasad1; Romain Huguet1; Eloy R. Wouters1 , 1Thermo Fisher Scientific, San Jose, CA; 2Rockford, IL ABSTRACT…

proteomics WHITE PAPER 65343 Crosslinking mass spectrometry (XL-MS) goes mainstream Mass spectrometrists and structural biologists guide to using XL-MS to understand the structure and function of molecular machines Author Executive summary Julian Saba, Thermo Fisher Scientific, San Jose, CA, USA…

An optimized enrichment strategy for improved mass spec analysis of chemically crosslinked peptides Leigh Foster1, Ryan Bomgarden1, Erum Raja1, Chris Etienne1, Rosa Viner2, John Rogers1 1Thermo Fisher Scientific, Rockford, IL; 2Thermo Fisher Scientific, San Jose, CA Heat Protein Recovery (Background…