Evaluation of FAIMS Technology for Mass Spec Analysis of Chemical Cross-linked Peptides

Optimization of FAIMS-XL-MS Workflow for Phospho-Enrichable Crosslinkers Yuqi Shi1, Brand D. Groppe2, Leigh Foster3, Ryan Bomgarden3, Rosa Viner1, Thermo Fisher Scientific, 1San Jose, CA, 2Kansas City, MO, 3Rockford, IL Previously we described optimized FAIMS XL-MS workflow for instruments with ion-funnel…

Thermo Scientific™ Proteome Discoverer™ using a XlinkX software node. Methods: Different amine-reactive, homobifuctional crosslinkers including disuccinimidyl Results: For both DSSO and BuUrBu, we identified over 40 BSA inter-crosslinked peptides 2 CID for DSSO. We also to less than(DSS), 20 using…

evaluated traditional non-cleavable and MS-cleavable crosslinkers for crosslinked peptide analysis using an Orbitrap Fusion Lumos mass spectrometer. For MS-cleavable crosslinkers, we also compared different types of fragmentation (CID, ETD) and levels of tandem mass spectrometry (MS2 vs. MS3). Our data…

Technical note | TN003979 Omics Proteome wide interactomics analysis using MS-cleavable crosslinkers and the Orbitrap Astral Zoom mass spectrometer Authors Goal Yi He , Tabiwang N. Arrey , Eugen Develop an end-to-end crosslinking mass spectrometry (XL-MS) workflow for MS-cleavable Damoc2,…