Crosslinking mass spectrometry (XL-MS) goes mainstream

Thermo Scientific™ Proteome Discoverer™ using a XlinkX software node. Methods: Different amine-reactive, homobifuctional crosslinkers including disuccinimidyl Results: For both DSSO and BuUrBu, we identified over 40 BSA inter-crosslinked peptides 2 CID for DSSO. We also to less than(DSS), 20 using…

Technical note | TN003979 Omics Proteome wide interactomics analysis using MS-cleavable crosslinkers and the Orbitrap Astral Zoom mass spectrometer Authors Goal Yi He , Tabiwang N. Arrey , Eugen Develop an end-to-end crosslinking mass spectrometry (XL-MS) workflow for MS-cleavable Damoc2,…

evaluated traditional non-cleavable and MS-cleavable crosslinkers for crosslinked peptide analysis using an Orbitrap Fusion Lumos mass spectrometer. For MS-cleavable crosslinkers, we also compared different types of fragmentation (CID, ETD) and levels of tandem mass spectrometry (MS2 vs. MS3). Our data…

Relative Quantification of TMT-labeled, cross-linked proteins using XlinkX node in Proteome Discoverer Rosa Viner1, Kai Fritzemeier2, Frank Berg2, Torsten Ueckert2, Leigh Foster3, Ryan Bomgarden3, Richard A. Scheltema4, Albert J.R. Heck4, Clinton Yu5, Lan Huang5; 1Thermo Fisher Scientific, San Jose, CA;…