Disulfide Bond Identification of Biotherapeutic Proteins Using Various Fragmentation Techniques Available on an Orbitrap Fusion Tribrid Mass Spectrometer

evaluated traditional non-cleavable and MS-cleavable crosslinkers for crosslinked peptide analysis using an Orbitrap Fusion Lumos mass spectrometer. For MS-cleavable crosslinkers, we also compared different types of fragmentation (CID, ETD) and levels of tandem mass spectrometry (MS2 vs. MS3). Our data…

Go beyond today’s discovery Orbitrap Eclipse Tribrid mass spectrometer Go beyond today’s discovery When complex analytical questions require a definitive answer, you need a powerful and versatile solution that will allow you to accurately resolve subtle differences, distinguish the right…

APPLICATION NOTE 74160 Proton transfer charge reduction (PTCR) improves spectral matching and sequence coverage in middle-down analysis of monoclonal antibodies Luca Fornelli,1 Ryan N. Oates,1 Kristina Srzentić,2 Christopher Mullen,3 John E. P. Syka,3 and Romain Huguet3 University of Oklahoma, Norman,…

Thermo Scientific™ Proteome Discoverer™ using a XlinkX software node. Methods: Different amine-reactive, homobifuctional crosslinkers including disuccinimidyl Results: For both DSSO and BuUrBu, we identified over 40 BSA inter-crosslinked peptides 2 CID for DSSO. We also to less than(DSS), 20 using…