Analysis of siRNA Duplexes at Non- Denaturing UPLC Conditions Using MaxPeak Premier Column Technology

Application Note Analysis of siRNA Drugs at Denaturing UPLC Conditions Using MaxPeak Premier Column Technology Irene Suarez Marina, Willy Verluyten, Evelien Dejaegere, Leslie Napoletano, J-P. Boon, Mario Hellings, Martin Gilar Janssen Pharmaceutical Companies of Johnson & Johnson, Waters Corporation Abstract…

Application Note Reproducible Hydrophilic Interaction Chromatography for Denaturing and NonDenaturing Analyses of Oligonucleotides Using GTxResolve Premier BEH Amide Columns Abraham S. Finny, Tatiana Johnston, Brent Sauner, Balasubrahmanyam Addepalli, Matthew A. Lauber Waters Corporation For research use only. Not for use…

[ APPLICATION NOTEBOOK ] APPLICATION SOLUTIONS FOR OLIGONUCLEOTIDES Table of Contents Introduction – UPLC Analysis of Synthetic Oligonucleotides......................................................................................................................... 4 [ HPLC & UPLC Separations ] Real-Time Analysis of RNAi Duplexes ....................................................................................................................................................................... 8 Semi-Preparative Scale Single-Stranded RNA Purification .......................................................................................................................... 11 Oligonucleotide…

Application Note Studying Mobile Phases for HILIC UV/MS Analysis of Oligonucleotide Therapeutics including an siRNA Duplex, a Lipid Conjugated ASO, and a CRISPR sgRNA Szabolcs Fekete, Makda Araya, Balasubrahmanyam Addepalli, Matthew A. Lauber Waters Corporation Abstract Hydrophilic interaction liquid chromatography…