Single-shot quantitative plasma proteomics using the PASEF method

High throughput 4D-Proteomics – Application of dia-PASEF® and the Evosep One for short gradients The timsTOF Pro offers a combination of two unique technologies, namely a 4th dimension provided by Trapped Ion Mobility Spectrometry (TIMS) to enhance ion separation and…

High throughput proteomics – Application of dia-PASEF and Evosep One for short gradients ASMS 2020, MP122 Stephanie Kaspar-Schoenefeld1, Markus Lubeck1, Thomas Kosinski1, Scarlet Koch1, Oliver Raether1, Gary Kruppa1 1Bruker Daltonik GmbH, 28359 Bremen, Germany Introduction Data-independent acquisition (DIA) promises reproducible…

Plasma proteomics goes high throughput – timsTOF Pro with PASEF and 4D feature alignment to quantify 500 plasma proteins in 11.5 min The timsTOF Pro with PASEF and the Evosep One for biomarker discovery in large sample cohorts of human…

dia-PASEF® applied on different gradient lengths Data Independent Acquisition (DIA) workflows have gained in popularity as they overcome the issue of stochastic selection of peptide precursors encountered in typical data-dependent approaches (DDA) and thereby promise reproducible and accurate protein identification…